Karousis Lab
@karousislab.bsky.social
Group leader in Bern, Cell-free translation enthusiast enjoying teaching, once upon @RNAKook on x-Twitter
https://scholar.google.com/citations?user=24B_5toAAAAJ&hl=en
https://www.ekarousis.net/
https://scholar.google.com/citations?user=24B_5toAAAAJ&hl=en
https://www.ekarousis.net/
Pinned
Karousis Lab
@karousislab.bsky.social
· Oct 13
A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal peptidyl transferase inhibitor
Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...
www.biorxiv.org
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal peptidyl transferase inhibitor
Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...
www.biorxiv.org
October 13, 2025 at 10:35 AM
🚀 New preprint!
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
We used a human cell-free translation screen (~28 000 compounds) to discover NT-2, a #Fusarium -derived #mycotoxin that blocks human ribosomes.
#Cryo-EM at 1.72 Å reveals a link between chemical inhibition and ribosome dormancy.
www.biorxiv.org/content/10.1...
It was a joy hosting @mandymuller.bsky.social in Bern!
I had a great time yesterday visiting the Department of Chemistry, Biochemistry, and Pharmaceutical Sciences at the University of Bern - thanks @karousislab.bsky.social for the invite!
September 30, 2025 at 8:47 PM
It was a joy hosting @mandymuller.bsky.social in Bern!
Reposted by Karousis Lab
Nature Reviews Methods Primers (2025): "Flow biocatalysis"
https://paradisiresearch.com/research-highlights/nature-reviews-methods-primers-2025-flow-biocatalysis/
https://paradisiresearch.com/research-highlights/nature-reviews-methods-primers-2025-flow-biocatalysis/
September 20, 2025 at 12:06 PM
Nature Reviews Methods Primers (2025): "Flow biocatalysis"
https://paradisiresearch.com/research-highlights/nature-reviews-methods-primers-2025-flow-biocatalysis/
https://paradisiresearch.com/research-highlights/nature-reviews-methods-primers-2025-flow-biocatalysis/
Reposted by Karousis Lab
Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm
Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm
Gade et al. report that polysomes and mRNA levels impact the biophysical properties of the cytoplasm. Using passive rheology probes and single-particle tracking, they demonstrate that intracellular diffusion is enhanced when polysomes are disassembled or when mRNA levels are reduced in eukaryotic cells.
dlvr.it
September 7, 2025 at 7:50 PM
Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm
*️⃣ Step-by-step STAR protocol for Cell-free translation and mRNA decay *️⃣
What to do (and what not to do 😅) guide based on our work on Nsp1 published in @cp-cellreports.bsky.social.
www.sciencedirect.com/science/arti...
@unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social @snf-fns.ch
What to do (and what not to do 😅) guide based on our work on Nsp1 published in @cp-cellreports.bsky.social.
www.sciencedirect.com/science/arti...
@unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social @snf-fns.ch
Protocol for monitoring mRNA translation and degradation in human cell-free lysates
Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation u…
www.sciencedirect.com
September 11, 2025 at 2:40 PM
*️⃣ Step-by-step STAR protocol for Cell-free translation and mRNA decay *️⃣
What to do (and what not to do 😅) guide based on our work on Nsp1 published in @cp-cellreports.bsky.social.
www.sciencedirect.com/science/arti...
@unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social @snf-fns.ch
What to do (and what not to do 😅) guide based on our work on Nsp1 published in @cp-cellreports.bsky.social.
www.sciencedirect.com/science/arti...
@unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social @snf-fns.ch
Excellent science, impeccable organisation and breathtaking views in and out the #RNA turnover #BiochemEvent in Brighton, UK 🌊🧬 @biochemsoc.bsky.social
August 30, 2025 at 8:56 AM
Excellent science, impeccable organisation and breathtaking views in and out the #RNA turnover #BiochemEvent in Brighton, UK 🌊🧬 @biochemsoc.bsky.social
Reposted by Karousis Lab
Very excited that our most significant work, a collaboration w/ Dr. Can Cenik at UT Austin on translational gene regulation, was finally published in Nature Biotechnology in a dual set of studies:
Paper 1 -- an AI model trained to predict translation rates from mRNA sequences: rdcu.be/exN1l
Paper 1 -- an AI model trained to predict translation rates from mRNA sequences: rdcu.be/exN1l
Predicting the translation efficiency of messenger RNA in mammalian cells
Nature Biotechnology - A deep convolutional neural network model predicts the influence of the full-length mRNA sequence on translation efficiency.
url.de.m.mimecastprotect.com
July 25, 2025 at 1:37 PM
Very excited that our most significant work, a collaboration w/ Dr. Can Cenik at UT Austin on translational gene regulation, was finally published in Nature Biotechnology in a dual set of studies:
Paper 1 -- an AI model trained to predict translation rates from mRNA sequences: rdcu.be/exN1l
Paper 1 -- an AI model trained to predict translation rates from mRNA sequences: rdcu.be/exN1l
Reposted by Karousis Lab
Translation efficiency covariation identifies conserved coordination patterns across cell types go.nature.com/3TXvmZy
Translation efficiency covariation identifies conserved coordination patterns across cell types - Nature Biotechnology
Matched ribosome profiling and RNA sequencing data quantify translation efficiency patterns across 140 human and mouse cell lines.
go.nature.com
July 25, 2025 at 7:10 PM
Translation efficiency covariation identifies conserved coordination patterns across cell types go.nature.com/3TXvmZy
🌊 Potential lab logo 🌊
July 24, 2025 at 12:39 PM
🌊 Potential lab logo 🌊
Reposted by Karousis Lab
New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...
July 12, 2025 at 12:10 AM
New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...
Reposted by Karousis Lab
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay - Nature Communications
Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...
www.nature.com
July 13, 2025 at 3:59 AM
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
What if I told you, there is a way to pause the time?
July 3, 2025 at 11:16 PM
What if I told you, there is a way to pause the time?
Great read from Akis and colleagues! @akispapantonis.bsky.social
New online! RNA polymerase II transcription compartments — from factories to condensates
RNA polymerase II transcription compartments — from factories to condensates
Nature Reviews Genetics, Published online: 19 June 2025; doi:10.1038/s41576-025-00859-6In this Review, Rippe and Papantonis describe advances in understanding the role of transcription compartments in gene regulation, specifically by collating and contrasting historical work on transcription factories with more recent work on transcriptional condensates.
www.nature.com
June 19, 2025 at 4:20 PM
Great read from Akis and colleagues! @akispapantonis.bsky.social
Reposted by Karousis Lab
A protein produced by the pathogenic bacteria Helicobacter pylori can block amyloid proteins from clustering, new #ScienceAdvances research finds.
Helicobacter pylori CagA protein is a potent and broad-spectrum amyloid inhibitor
Helicobacter pylori CagA protein strongly inhibits functional and pathogenic amyloid fibril formation in both bacteria and humans.
scim.ag
June 19, 2025 at 4:09 PM
A protein produced by the pathogenic bacteria Helicobacter pylori can block amyloid proteins from clustering, new #ScienceAdvances research finds.
Our method paper to produce efficient cell-free translation lysates from diverse human cell types is out 🎉 www.jbc.org/article/S002... @unibe.ch @mcid-unibe.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
May 28, 2025 at 4:13 PM
Our method paper to produce efficient cell-free translation lysates from diverse human cell types is out 🎉 www.jbc.org/article/S002... @unibe.ch @mcid-unibe.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
@muehlemann.bsky.social @nickkouvelas.bsky.social
I am very excited and grateful for the opportunity to present our work and exchange ideas with the research community at LMU Munich hosted by Jonathan Bohlen! @jonathanbohlen.bsky.social @jonathanbohlen.bsky.social @unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social
We're happy to welcome Evangelos Karousis next Monday! 🎉
He’ll be talking about “Decoding viral secrets using cell-free translation” 🧬
📅 May 12 🕐 1:15 PM ➡️Lynen Lecture Hall
Don’t miss it! 🤗✨
@karousislab.bsky.social @jonathanbohlen.bsky.social
@unibe.ch
He’ll be talking about “Decoding viral secrets using cell-free translation” 🧬
📅 May 12 🕐 1:15 PM ➡️Lynen Lecture Hall
Don’t miss it! 🤗✨
@karousislab.bsky.social @jonathanbohlen.bsky.social
@unibe.ch
May 8, 2025 at 8:17 AM
I am very excited and grateful for the opportunity to present our work and exchange ideas with the research community at LMU Munich hosted by Jonathan Bohlen! @jonathanbohlen.bsky.social @jonathanbohlen.bsky.social @unibe.ch @mcid-unibe.bsky.social @nccr-rna-disease.bsky.social
April 6, 2025 at 9:32 PM
Reposted by Karousis Lab
🦠😷 Researchers at the #unibern have gained new insights into an early mechanism of #coronavirus infection. 👉 mediarelations.unibe.ch/media_releas...
@karousislab.bsky.social @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social
Image: © Adrian Bothe
@karousislab.bsky.social @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social
Image: © Adrian Bothe
April 1, 2025 at 11:30 AM
🦠😷 Researchers at the #unibern have gained new insights into an early mechanism of #coronavirus infection. 👉 mediarelations.unibe.ch/media_releas...
@karousislab.bsky.social @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social
Image: © Adrian Bothe
@karousislab.bsky.social @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social
Image: © Adrian Bothe
I think I study more #Biochemistry on semester weekends than my students 😅
Gluconeogenesis feat. Greek language lessons 🍭
Gluconeogenesis feat. Greek language lessons 🍭
March 30, 2025 at 3:29 PM
I think I study more #Biochemistry on semester weekends than my students 😅
Gluconeogenesis feat. Greek language lessons 🍭
Gluconeogenesis feat. Greek language lessons 🍭
Reposted by Karousis Lab
The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition
The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition
Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA degradation independently of translation, whereas MERS-CoV Nsp1 inhibits translation without degradation, revealing distinct mechanisms and co-evolutionary protection of viral mRNAs.
dlvr.it
March 29, 2025 at 12:36 PM
The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition
Reposted by Karousis Lab
A Perspective in Nature Reviews Neuroscience focuses on neural resonance theory in music, which summarizes how the dynamics of fundamental neural mechanisms can explain various aspects of music perception and performance. https://go.nature.com/3DXLLJ2 🔒
March 29, 2025 at 7:17 PM
A Perspective in Nature Reviews Neuroscience focuses on neural resonance theory in music, which summarizes how the dynamics of fundamental neural mechanisms can explain various aspects of music perception and performance. https://go.nature.com/3DXLLJ2 🔒
Our latest research on #Coronavirus Nsp1 found its home (and a new name) at @cp-cellreports.bsky.social www.cell.com/cell-reports... Thankful to the first (dream) team of my lab and funding for making this happen. @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social @unibe.ch
The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition
Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition
and mRNA degradation using a human cell-free translation system. They demonstrate
that SARS-CoV-2 Nsp1 mediates mRNA ...
www.cell.com
March 28, 2025 at 5:00 PM
Our latest research on #Coronavirus Nsp1 found its home (and a new name) at @cp-cellreports.bsky.social www.cell.com/cell-reports... Thankful to the first (dream) team of my lab and funding for making this happen. @nccr-rna-disease.bsky.social @mcid-unibe.bsky.social @unibe.ch
Reposted by Karousis Lab
Cellular responses to RNA damage: Cell www.cell.com/cell/fulltex...
Cellular responses to RNA damage
RNA plays a central role in protein biosynthesis and performs diverse regulatory and
catalytic functions, making it essential for all processes of life. Like DNA, RNA
is constantly subjected to damage...
www.cell.com
February 22, 2025 at 3:23 PM
Cellular responses to RNA damage: Cell www.cell.com/cell/fulltex...