Maurice Lee
@mauriceylee.bsky.social
PhD in BioPhysics.
Scientist who worked at the Genome Institute of Singapore. Worked on 3D lightsheet and confocal microscopy for 3D spatial transcriptomics via multiplexed error robust fluorescence in situ hybridisation (MERFISH).
Scientist who worked at the Genome Institute of Singapore. Worked on 3D lightsheet and confocal microscopy for 3D spatial transcriptomics via multiplexed error robust fluorescence in situ hybridisation (MERFISH).
Reposted by Maurice Lee
The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out.
It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.
We used it to study cancer-immune cell interactions.
www.nature.com/articles/s41...
It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.
We used it to study cancer-immune cell interactions.
www.nature.com/articles/s41...
Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy
Nature Methods - A self-driving multiresolution light-sheet microscope enables the simultaneous observation and quantification of cellular and subcellular dynamics in the context of intact and...
www.nature.com
February 12, 2025 at 1:45 PM
The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out.
It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.
We used it to study cancer-immune cell interactions.
www.nature.com/articles/s41...
It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.
We used it to study cancer-immune cell interactions.
www.nature.com/articles/s41...
Goodbye Mr Snouty. I had a lot of fun working with you. It has been a blast. Hopefully the next scientist/engineer who works with you will enjoy the process too.🥹
February 3, 2025 at 6:47 AM
Goodbye Mr Snouty. I had a lot of fun working with you. It has been a blast. Hopefully the next scientist/engineer who works with you will enjoy the process too.🥹
Reposted by Maurice Lee
Science bio-imaging question:
What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?
What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?
January 14, 2025 at 7:56 PM
Science bio-imaging question:
What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?
What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?
Hello science friends on bluesky!
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.
January 13, 2025 at 4:34 AM
Hello science friends on bluesky!
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.
Reposted by Maurice Lee
Also in today's preprint trawling: imaging at depth is difficult, so let's just... squish cells? arxiv.org/abs/2411.12656
November 20, 2024 at 10:40 AM
Also in today's preprint trawling: imaging at depth is difficult, so let's just... squish cells? arxiv.org/abs/2411.12656
Reposted by Maurice Lee
Does anyone know any good publications/databases to find non-coding variant loci for neurodevelopmental disorders? (anything, but particularly disorders of brain size if possible)
November 18, 2024 at 12:53 PM
Does anyone know any good publications/databases to find non-coding variant loci for neurodevelopmental disorders? (anything, but particularly disorders of brain size if possible)
Reposted by Maurice Lee
The pSABER paper is now online!
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods
pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...
doi.org
November 15, 2024 at 10:27 PM
The pSABER paper is now online!
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
Caring for your friends in science means lending them your neutral density filter wheel to see if it will help to alleviate their microscope problems
November 15, 2024 at 5:30 AM
Caring for your friends in science means lending them your neutral density filter wheel to see if it will help to alleviate their microscope problems
please don't do this.
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm
November 14, 2024 at 11:10 AM
please don't do this.
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm
Reposted by Maurice Lee
Calling all super-resolution microscopy enthusiasts!
Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.
Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!
Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.
Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!
November 14, 2024 at 10:57 AM
Calling all super-resolution microscopy enthusiasts!
Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.
Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!
Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.
Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!
Hello everyone! So many of you have followed me over the past several days. I'm guessing most are interested in my science research work (i.e., fluorescence microscopy, image analysis, spatial transcriptomics). So I decided to wind down my bird app account and slowly post a little more on Bluesky.
November 14, 2024 at 7:58 AM
Hello everyone! So many of you have followed me over the past several days. I'm guessing most are interested in my science research work (i.e., fluorescence microscopy, image analysis, spatial transcriptomics). So I decided to wind down my bird app account and slowly post a little more on Bluesky.
Reposted by Maurice Lee
Made a starter pack for microscopy and image analysis - please (self-)nominate additions!
go.bsky.app/Nwa91pC
go.bsky.app/Nwa91pC
November 6, 2024 at 4:32 PM
Made a starter pack for microscopy and image analysis - please (self-)nominate additions!
go.bsky.app/Nwa91pC
go.bsky.app/Nwa91pC
Reposted by Maurice Lee
For folks just arriving in #AcademicBsky or #ScienceBsky, here are a couple of Biophysics starter packs. Biophysicists, pls tag yourself and others and I will add you to the new starter packs. go.bsky.app/4AUjqVz
November 7, 2024 at 1:02 PM
For folks just arriving in #AcademicBsky or #ScienceBsky, here are a couple of Biophysics starter packs. Biophysicists, pls tag yourself and others and I will add you to the new starter packs. go.bsky.app/4AUjqVz
I had the opportunity to conduct a hands-on workshop on basic optical alignment today!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
November 9, 2023 at 9:12 AM
I had the opportunity to conduct a hands-on workshop on basic optical alignment today!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
The 21st Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications hosted by the CRL Molecular Imaging Center at UC Berkeley on 23-25 Jan 2024.
www.eventbrite.com/e/21st-annua...
www.eventbrite.com/e/21st-annua...
21st Annual Advanced Imaging Methods Workshop
The Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications.
www.eventbrite.com
November 1, 2023 at 8:23 AM
The 21st Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications hosted by the CRL Molecular Imaging Center at UC Berkeley on 23-25 Jan 2024.
www.eventbrite.com/e/21st-annua...
www.eventbrite.com/e/21st-annua...
I think these are my favorite forever stamps ever!🖤🤍♥️💚🩵🧡
October 4, 2023 at 9:29 AM
I think these are my favorite forever stamps ever!🖤🤍♥️💚🩵🧡
AI powered microscope to spot cancer? Built by Google and DOD?
www.cnbc.com/2023/09/18/g...
www.cnbc.com/2023/09/18/g...
Google and the Department of Defense are building an AI-powered microscope to help doctors spot canc...
The AI-powered tool is called an Augmented Reality Microscope, or ARM, and Google and the Department of Defense have been quietly working on it for years.
www.cnbc.com
September 19, 2023 at 9:09 AM
AI powered microscope to spot cancer? Built by Google and DOD?
www.cnbc.com/2023/09/18/g...
www.cnbc.com/2023/09/18/g...
Singapore's a blue zone city!?!?! I guess my life expectancy is ~81
finance.yahoo.com/news/longevi...
finance.yahoo.com/news/longevi...
The longevity secrets of Singapore, the 6th blue zone city where people are living the longest, happ...
The five health and happiness secrets of Singapore, "blue zone 2.0—the next frontier of aging."
finance.yahoo.com
August 31, 2023 at 2:41 PM
Singapore's a blue zone city!?!?! I guess my life expectancy is ~81
finance.yahoo.com/news/longevi...
finance.yahoo.com/news/longevi...
We have started to work on 3D spatial transcriptomics of the whole larval zebrafish brain and I'm excited to share an image that we recently acquired in the lab!
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
August 19, 2023 at 1:47 AM
We have started to work on 3D spatial transcriptomics of the whole larval zebrafish brain and I'm excited to share an image that we recently acquired in the lab!
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
"By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain."
https://www.nature.com/articles/d41586-023-02559-9
https://www.nature.com/articles/d41586-023-02559-9
The quest to map the mouse brain
By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain. By combining single-cell ...
www.nature.com
August 16, 2023 at 4:01 PM
"By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain."
https://www.nature.com/articles/d41586-023-02559-9
https://www.nature.com/articles/d41586-023-02559-9
Dr Waters is searching for an advanced microscopy fellow!
https://microfellows.hms.harvard.edu/apply/
https://microfellows.hms.harvard.edu/apply/
microfellows.hms.harvard.edu
August 8, 2023 at 6:06 AM
Dr Waters is searching for an advanced microscopy fellow!
https://microfellows.hms.harvard.edu/apply/
https://microfellows.hms.harvard.edu/apply/
Yes! I feel like I'm working on a technology of the future now.
"we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)"
https://www.science.org/doi/10.1126/science.abq4964
"we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)"
https://www.science.org/doi/10.1126/science.abq4964
Just a moment...
www.science.org
August 7, 2023 at 3:16 AM
Yes! I feel like I'm working on a technology of the future now.
"we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)"
https://www.science.org/doi/10.1126/science.abq4964
"we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)"
https://www.science.org/doi/10.1126/science.abq4964
I'm still amazed that my Pixel 7 can see stars with the astrophotography mode amidst all that light pollution in Singapore.
August 4, 2023 at 2:49 PM
I'm still amazed that my Pixel 7 can see stars with the astrophotography mode amidst all that light pollution in Singapore.
It was a sunny day at #BiopolisSingapore
August 4, 2023 at 1:51 PM
It was a sunny day at #BiopolisSingapore