Scientist who worked at the Genome Institute of Singapore. Worked on 3D lightsheet and confocal microscopy for 3D spatial transcriptomics via multiplexed error robust fluorescence in situ hybridisation (MERFISH).
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.
The #1.5 glass coverslips that we have are all thicker than the 170 microns I expected. The range is supposed to be 150-190 microns. And different brands of coverslips have slightly different distributions too!
The #1.5 glass coverslips that we have are all thicker than the 170 microns I expected. The range is supposed to be 150-190 microns. And different brands of coverslips have slightly different distributions too!
The first two were 0.183 and 0.212 mm thick.🤷🏻♂️
The first two were 0.183 and 0.212 mm thick.🤷🏻♂️