Mahipal Ganji
@ganjilab.bsky.social
Assistant professor at IISc Bangalore. Interested in DNA nanotechnology, single-molecule biophysics, super-resolution imaging, chromatin organization.
Lab webpage: www.ganjilab.org
Lab webpage: www.ganjilab.org
Reposted by Mahipal Ganji
Excited to share a new preprint from a team of researchers I am part of (funded by an NIH grant). The paper helps us understand a question I have been interested in for a while: why are bacteria so resilient?
www.biorxiv.org/content/10.1...
@meyerroc.bsky.social
@juliebiteenlab.bsky.social
www.biorxiv.org/content/10.1...
@meyerroc.bsky.social
@juliebiteenlab.bsky.social
October 22, 2025 at 7:21 PM
Excited to share a new preprint from a team of researchers I am part of (funded by an NIH grant). The paper helps us understand a question I have been interested in for a while: why are bacteria so resilient?
www.biorxiv.org/content/10.1...
@meyerroc.bsky.social
@juliebiteenlab.bsky.social
www.biorxiv.org/content/10.1...
@meyerroc.bsky.social
@juliebiteenlab.bsky.social
Reposted by Mahipal Ganji
(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen!
Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵
Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵
August 13, 2025 at 2:19 PM
(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen!
Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵
Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵
Thank you for having me here, Mike. Big thanks to all the organizers. It was fantastic meeting. Looking forward to the next editions of #SMLMS2025
Next on stage is Mahipal Ganji from the IIT Bangalore @ganjilab.bsky.social shares great work on stacking interactions in DNA/RNA and multplexed-DNA PAINT to measure nuclear ultrastructure #SMLMS2025
August 30, 2025 at 12:53 AM
Thank you for having me here, Mike. Big thanks to all the organizers. It was fantastic meeting. Looking forward to the next editions of #SMLMS2025
Reposted by Mahipal Ganji
📢 Dear lovers of prokaryotic chromosomes:
Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳.
Join us!
@biophychrom.bsky.social
@danielabarilla.bsky.social
@damelab.bsky.social
@graingerlab.bsky.social
@biochemsoc.bsky.social
#microsky
Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳.
Join us!
@biophychrom.bsky.social
@danielabarilla.bsky.social
@damelab.bsky.social
@graingerlab.bsky.social
@biochemsoc.bsky.social
#microsky
July 30, 2025 at 10:20 AM
📢 Dear lovers of prokaryotic chromosomes:
Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳.
Join us!
@biophychrom.bsky.social
@danielabarilla.bsky.social
@damelab.bsky.social
@graingerlab.bsky.social
@biochemsoc.bsky.social
#microsky
Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳.
Join us!
@biophychrom.bsky.social
@danielabarilla.bsky.social
@damelab.bsky.social
@graingerlab.bsky.social
@biochemsoc.bsky.social
#microsky
Reposted by Mahipal Ganji
Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫
www.eventsforce.net/biochemsoc/f...
@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social
www.eventsforce.net/biochemsoc/f...
@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social
July 25, 2025 at 12:14 PM
Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫
www.eventsforce.net/biochemsoc/f...
@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social
www.eventsforce.net/biochemsoc/f...
@vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social
Reposted by Mahipal Ganji
And the story is published now!🍾
www.nature.com/articles/s41...
Open access link: rdcu.be/exnOc
Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social
Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏
#microsky #archaeasky
www.nature.com/articles/s41...
Open access link: rdcu.be/exnOc
Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social
Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏
#microsky #archaeasky
July 22, 2025 at 9:06 PM
And the story is published now!🍾
www.nature.com/articles/s41...
Open access link: rdcu.be/exnOc
Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social
Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏
#microsky #archaeasky
www.nature.com/articles/s41...
Open access link: rdcu.be/exnOc
Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social
Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏
#microsky #archaeasky
Very much looking forward for #SMLMS2025
June 27, 2025 at 4:49 PM
Very much looking forward for #SMLMS2025
Reposted by Mahipal Ganji
🔬 Are you working with single-molecule super-resolution methods (SMLM, MINFLUX) and would like to meet experts in the field and discuss your work? 👉🏻 consider attending the SMLMS 2025 Meeting in Bonn 👉🏻 abstract submission is open until June 27th 👉🏻 www.smlms.org @smlm-symposium.bsky.social
June 12, 2025 at 5:57 AM
🔬 Are you working with single-molecule super-resolution methods (SMLM, MINFLUX) and would like to meet experts in the field and discuss your work? 👉🏻 consider attending the SMLMS 2025 Meeting in Bonn 👉🏻 abstract submission is open until June 27th 👉🏻 www.smlms.org @smlm-symposium.bsky.social
Such fantastic news! I had the privilege of working with Cees — an incredibly inspiring scientist. His research truly deserves this recognition. Huge congratulations, Cees!
Wow, I feel greatly honored by being awarded the 2026 Biochemical Society Award for Significant Breakthrough or Achievement, as was just announced!
www.biochemistry.org/about-us/new...
It is wonderful that the Biochemical Society honors our contributions to single-molecule biophysics in this way!
www.biochemistry.org/about-us/new...
It is wonderful that the Biochemical Society honors our contributions to single-molecule biophysics in this way!
The 2026 Biochemical Society Award for Significant Breakthrough or Achievement is presented to Cees Dekker @ceesdekker.bsky.social! Celebrating his ground-breaking contributions to nanotechnology, Cees has pioneered research on solid-state nanopores and nanopore-based DNA sequencing.
April 8, 2025 at 9:17 AM
Such fantastic news! I had the privilege of working with Cees — an incredibly inspiring scientist. His research truly deserves this recognition. Huge congratulations, Cees!
Reposted by Mahipal Ganji
Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!
www.cell.com/current-biol...
www.cell.com/current-biol...
Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus
Adhikashreni et al. use quantitative live-cell imaging to track cell-cycle dynamics
in hundreds of individual bacteria. They observe nutrient-dependent regulation of
replication elongation rates that ...
www.cell.com
April 4, 2025 at 3:57 PM
Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!
www.cell.com/current-biol...
www.cell.com/current-biol...
Reposted by Mahipal Ganji
Latest #CDlab paper online in @labonachip.rsc.org: pubs.rsc.org/en/content/a...
Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!
Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!
March 3, 2025 at 9:13 AM
Latest #CDlab paper online in @labonachip.rsc.org: pubs.rsc.org/en/content/a...
Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!
Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!
Reposted by Mahipal Ganji
Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?
March 1, 2025 at 1:57 PM
Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?
Reposted by Mahipal Ganji
In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...
Future Directions of the Prokaryotic Chromosome Field
In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of ....
onlinelibrary.wiley.com
February 20, 2025 at 8:14 PM
In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...
Reposted by Mahipal Ganji
ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬
The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy
www.biorxiv.org/content/10.1...
The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy
www.biorxiv.org/content/10.1...
February 10, 2025 at 8:21 AM
ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬
The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy
www.biorxiv.org/content/10.1...
The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy
www.biorxiv.org/content/10.1...
Reposted by Mahipal Ganji
The kindest of gentle reminders. We have one week to go on one #PhDPosition and one #PostdocPosition in single-molecule dielectrophoresis. Do you like single molecules , advanced optics and nanotechnology? More details in the thread👇 Dear Bluesky poplulation, please help with reposting
Hi all, I am very excited to share two positions (PhD and PostDoc) in my group on a EU-funded project ProAct.
More about the positions below.
Please share with your colleagues! This will be a great project to work on and also we offer a great environment at the BIOS group
More about the positions below.
Please share with your colleagues! This will be a great project to work on and also we offer a great environment at the BIOS group
February 6, 2025 at 10:53 AM
The kindest of gentle reminders. We have one week to go on one #PhDPosition and one #PostdocPosition in single-molecule dielectrophoresis. Do you like single molecules , advanced optics and nanotechnology? More details in the thread👇 Dear Bluesky poplulation, please help with reposting
Reposted by Mahipal Ganji
Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1...
▶️40-fold flanking sequence effect on TF binding
▶️Motif recognition beats kinetics
▶️Minutes-long residence times
▶️Models of TF-nucleosome competiton
▶️40-fold flanking sequence effect on TF binding
▶️Motif recognition beats kinetics
▶️Minutes-long residence times
▶️Models of TF-nucleosome competiton
February 1, 2025 at 5:58 PM
Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1...
▶️40-fold flanking sequence effect on TF binding
▶️Motif recognition beats kinetics
▶️Minutes-long residence times
▶️Models of TF-nucleosome competiton
▶️40-fold flanking sequence effect on TF binding
▶️Motif recognition beats kinetics
▶️Minutes-long residence times
▶️Models of TF-nucleosome competiton
This is a fantastic community to be part of. Don’t miss the chance!
If you're interested in chromosome biology (DNA folding, gene regulation, replication, polymer modelling and more) we're organising the below this autumn. It'd be great to see you there. Please share if you can. :-)
January 22, 2025 at 1:56 AM
This is a fantastic community to be part of. Don’t miss the chance!
Amazing!
New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today:
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
January 17, 2025 at 7:39 AM
Amazing!
Reposted by Mahipal Ganji
🔬 Ever wondered how therapeutic antibodies work?
3D LLS-TDI-DNA-PAINT and live-cell LLS microscopy allow decoding of the molecular interplay of endogenous CD20 on B cells with therapeutic antibodies.
📄 Read more: www.science.org/doi/10.1126/...
3D LLS-TDI-DNA-PAINT and live-cell LLS microscopy allow decoding of the molecular interplay of endogenous CD20 on B cells with therapeutic antibodies.
📄 Read more: www.science.org/doi/10.1126/...
January 10, 2025 at 9:00 AM
🔬 Ever wondered how therapeutic antibodies work?
3D LLS-TDI-DNA-PAINT and live-cell LLS microscopy allow decoding of the molecular interplay of endogenous CD20 on B cells with therapeutic antibodies.
📄 Read more: www.science.org/doi/10.1126/...
3D LLS-TDI-DNA-PAINT and live-cell LLS microscopy allow decoding of the molecular interplay of endogenous CD20 on B cells with therapeutic antibodies.
📄 Read more: www.science.org/doi/10.1126/...
Really excited to share our latest preprint in collaboration with Sandeep Choubey's lab at IMSc. Mycobacterium tuberculosis Nucleoid Associated protein Lsr2 and DNA undergo sequence-dependent co-condensation. Check it out here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosis
The xenogeneic silencer protein Lsr2 from Mycobacterium tuberculosis plays a critical role in its survival and pathogenesis. Lsr2 is a nucleoid-associated protein that interacts with DNA in vivo and r...
www.biorxiv.org
January 7, 2025 at 10:18 AM
Really excited to share our latest preprint in collaboration with Sandeep Choubey's lab at IMSc. Mycobacterium tuberculosis Nucleoid Associated protein Lsr2 and DNA undergo sequence-dependent co-condensation. Check it out here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Mahipal Ganji
From the Henriques lab comes NanoPyx, which optimizes bioimage analysis by dynamically selecting code variations based on input data and hardware to ensures maximum computational efficiency.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Efficiently accelerated bioimage analysis with NanoPyx, a Liquid Engine-powered Python framework - Nature Methods
NanoPyx optimizes bioimage analysis workflows by dynamically selecting code variations based on input data and hardware. This ensures maximum computational efficiency, enabling fast analysis of large ...
www.nature.com
January 3, 2025 at 4:21 PM
From the Henriques lab comes NanoPyx, which optimizes bioimage analysis by dynamically selecting code variations based on input data and hardware to ensures maximum computational efficiency.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
High-speed 10-plex DNA-PAINT with a larger sequence repertoire
DNA-Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) enables multiplexed super-resolution imaging of biological samples. We expand the repertoire of speed-optimized DNA sequences fo...
www.biorxiv.org
January 3, 2025 at 5:57 AM
Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Mahipal Ganji
Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇
May 20, 2024 at 2:19 PM
Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇
Reposted by Mahipal Ganji
Our lab has an opening for a core member of our team: a talented senior scientist fascinated by single-molecule biophysics with a focus on novel instrumentation and experimentation. See the ad here: my.corehr.com/pls/uoxrecru...
Deadline for applications: February 14, 2025.
Deadline for applications: February 14, 2025.
Job Details
my.corehr.com
December 22, 2024 at 2:12 PM
Our lab has an opening for a core member of our team: a talented senior scientist fascinated by single-molecule biophysics with a focus on novel instrumentation and experimentation. See the ad here: my.corehr.com/pls/uoxrecru...
Deadline for applications: February 14, 2025.
Deadline for applications: February 14, 2025.