Sven Klumpe
@svenklumpe.bsky.social
CryoFIB Development and In situ structural biology. NPC and TE enthusiast. Drosophilist but ready to switch to wherever biology takes us
Getting to enjoy science with a group at IMBA/IMP of the Vienna BioCenter https://www.imp.ac.at/groups/sven-klumpe
Getting to enjoy science with a group at IMBA/IMP of the Vienna BioCenter https://www.imp.ac.at/groups/sven-klumpe
Pinned
Sven Klumpe
@svenklumpe.bsky.social
· Mar 5
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
Reposted by Sven Klumpe
Are you an early-stage graduate student (2nd or 3rd year) or early-stage postdoc based in the US or Canada, working primarily in Drosophila? Would you like to help improve the experience of all trainees working in Drosophila research? If so, read on.
(Please repost to reach a broad audience.)
(Please repost to reach a broad audience.)
November 12, 2025 at 4:49 AM
Are you an early-stage graduate student (2nd or 3rd year) or early-stage postdoc based in the US or Canada, working primarily in Drosophila? Would you like to help improve the experience of all trainees working in Drosophila research? If so, read on.
(Please repost to reach a broad audience.)
(Please repost to reach a broad audience.)
Join us at the Vienna BioCenter and @impvienna.bsky.social! Full disclaimer: I’m obviously biased but: mind-blowing facilities (!!!) and a great collaborative research culture make this an ideal place for anyone; especially suited to build up a lab. Plus Vienna is an amazing city to live in 😊
November 10, 2025 at 9:30 PM
Join us at the Vienna BioCenter and @impvienna.bsky.social! Full disclaimer: I’m obviously biased but: mind-blowing facilities (!!!) and a great collaborative research culture make this an ideal place for anyone; especially suited to build up a lab. Plus Vienna is an amazing city to live in 😊
Reposted by Sven Klumpe
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
November 6, 2025 at 7:38 AM
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Reposted by Sven Klumpe
Excited to share our new @narjournal.bsky.social paper!
Using ReLo and AlphaFold, we described a network of interactions between Drosophila piRNA pathway factors suggesting new links how they coordinate to ensure transposon silencing and genome integrity.
academic.oup.com/nar/article/...
Using ReLo and AlphaFold, we described a network of interactions between Drosophila piRNA pathway factors suggesting new links how they coordinate to ensure transposon silencing and genome integrity.
academic.oup.com/nar/article/...
October 18, 2025 at 6:59 AM
Excited to share our new @narjournal.bsky.social paper!
Using ReLo and AlphaFold, we described a network of interactions between Drosophila piRNA pathway factors suggesting new links how they coordinate to ensure transposon silencing and genome integrity.
academic.oup.com/nar/article/...
Using ReLo and AlphaFold, we described a network of interactions between Drosophila piRNA pathway factors suggesting new links how they coordinate to ensure transposon silencing and genome integrity.
academic.oup.com/nar/article/...
Really awesome findings, congrats!
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Antiviral reverse transcriptases reveal the evolutionary origin of telomerase
Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...
www.biorxiv.org
October 17, 2025 at 9:57 PM
Really awesome findings, congrats!
Congrats, Ulrich! Looking forward to see the amazing science your lab will do. Highly recommended for anyone searching for a PhD position!
Thrilled to share that I’ll be joining @imbmainz.bsky.social in February 2026 to start my own group!
We will explore new mechanisms in eukaryotic gene expression, leveraging ‘evolutionary play’ to uncover how regulation, repurposing, and hijacking shape RNA biology.
PhD positions available!
We will explore new mechanisms in eukaryotic gene expression, leveraging ‘evolutionary play’ to uncover how regulation, repurposing, and hijacking shape RNA biology.
PhD positions available!
October 13, 2025 at 8:07 PM
Congrats, Ulrich! Looking forward to see the amazing science your lab will do. Highly recommended for anyone searching for a PhD position!
So good I’m almost worried it is fake but anyhow an amazing message to start the week to 😊
Dr. Jane Goodall filmed an interview with Netflix in March 2025 that she understood would only be released after her death.
October 6, 2025 at 6:41 AM
So good I’m almost worried it is fake but anyhow an amazing message to start the week to 😊
Reposted by Sven Klumpe
🎉Congrats to our @vdeneke.bsky.social, postdoc in the @pauligroup.bsky.social, who has received the 2025 Life Sciences Research Award Austria from the ÖGMBT!
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
September 26, 2025 at 8:41 AM
🎉Congrats to our @vdeneke.bsky.social, postdoc in the @pauligroup.bsky.social, who has received the 2025 Life Sciences Research Award Austria from the ÖGMBT!
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
Reposted by Sven Klumpe
Congratulations to @vdeneke.bsky.social & Andreas Blaha @impvienna.bsky.social for their research on vertebrate fertilization, which was awarded this year's ÖGMBT Life Science Research Award in Austria for basic research!
🎉Congrats to our @vdeneke.bsky.social, postdoc in the @pauligroup.bsky.social, who has received the 2025 Life Sciences Research Award Austria from the ÖGMBT!
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
She's recognised for her groundbreaking work on the molecular mechanisms of fertilisation: www.imp.ac.at/news/article...
October 5, 2025 at 8:46 PM
Congratulations to @vdeneke.bsky.social & Andreas Blaha @impvienna.bsky.social for their research on vertebrate fertilization, which was awarded this year's ÖGMBT Life Science Research Award in Austria for basic research!
Reposted by Sven Klumpe
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
September 26, 2025 at 2:28 AM
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
Reposted by Sven Klumpe
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
September 23, 2025 at 5:08 PM
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
Reposted by Sven Klumpe
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Sven Klumpe
BIG NEWS!! We will be hiring a new professor with 25 years of endowed funding at the @biozentrum.unibas.ch @unibas.ch, focused on using biophysics and structural biology to explore climate-related fundamental science topics. Come start an innovative research cluster with us! 🧪🔬🧶🧬🌾🌍
The University of Basel is setting up a new endowed professorship at the @biozentrum to make fundamental discoveries at the interface between biophysics and climate-relevant environmental biology. This will be made possible by the Georg H. Endress Foundation.
New endowed professorship strengthens climate research at the University of Basel
The University of Basel is setting up a new endowed professorship at the Biozentrum to make fundamental discoveries at the interface between b...
www.unibas.ch
August 26, 2025 at 9:31 PM
BIG NEWS!! We will be hiring a new professor with 25 years of endowed funding at the @biozentrum.unibas.ch @unibas.ch, focused on using biophysics and structural biology to explore climate-related fundamental science topics. Come start an innovative research cluster with us! 🧪🔬🧶🧬🌾🌍
Reposted by Sven Klumpe
+1 would sign - I used FlyBase *regularly* for my undergrad thesis on Drosophila genetics!
August 23, 2025 at 11:21 PM
+1 would sign - I used FlyBase *regularly* for my undergrad thesis on Drosophila genetics!
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
August 23, 2025 at 11:21 PM
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
So awesome this is out in its final form. Congrats @jennysachweh.bsky.social , @becklab.bsky.social and team. Happy I could contribute a little to the story 😊
My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
The small GTPase Ran defines nuclear pore complex asymmetry
Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…
www.sciencedirect.com
August 18, 2025 at 8:32 PM
So awesome this is out in its final form. Congrats @jennysachweh.bsky.social , @becklab.bsky.social and team. Happy I could contribute a little to the story 😊
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
August 15, 2025 at 4:09 PM
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
Reposted by Sven Klumpe
FlyBase, a Drosophila database, will lose a third of its team in early October because the Harvard grant that covered the employees’ salaries was canceled. Scientists warn that losing FlyBase could devastate fly research.
By @claudia-lopez.bsky.social
www.thetransmitter.org/community/ha...
By @claudia-lopez.bsky.social
www.thetransmitter.org/community/ha...
Harvard University lays off fly database team
The layoffs jeopardize this resource, which has served more than 4,000 labs for about three decades.
www.thetransmitter.org
August 13, 2025 at 7:32 PM
FlyBase, a Drosophila database, will lose a third of its team in early October because the Harvard grant that covered the employees’ salaries was canceled. Scientists warn that losing FlyBase could devastate fly research.
By @claudia-lopez.bsky.social
www.thetransmitter.org/community/ha...
By @claudia-lopez.bsky.social
www.thetransmitter.org/community/ha...
Behind the paper article by Manel Llado Santaeularia on our manuscript on the in cell structure of a transposable element with @juliusbrennecke.bsky.social. Looking forward to exciting years @viennabiocenter.bsky.social Special thanks @sramundo.bsky.social for making this interaction possible!
July 18, 2025 at 6:37 AM
Behind the paper article by Manel Llado Santaeularia on our manuscript on the in cell structure of a transposable element with @juliusbrennecke.bsky.social. Looking forward to exciting years @viennabiocenter.bsky.social Special thanks @sramundo.bsky.social for making this interaction possible!
Reposted by Sven Klumpe
Exactly two weeks left to submit your abstract to the EMBO Mobile Genome Workshop.
☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th
☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th
⏰ Abstract deadline for 'The mobile genome' is 29 July!
👉 https://s.embl.org/mge25-01-bl
Join us 4–7 Nov 2025 at EMBL Heidelberg (or online) to explore the impact of TEs across biology. 🧬🔍
⭐🧑🏼🔬 24 talks + 15 flash talks from posters – don't miss out!
#EMBOMobileGenome
👉 https://s.embl.org/mge25-01-bl
Join us 4–7 Nov 2025 at EMBL Heidelberg (or online) to explore the impact of TEs across biology. 🧬🔍
⭐🧑🏼🔬 24 talks + 15 flash talks from posters – don't miss out!
#EMBOMobileGenome
July 15, 2025 at 12:35 PM
Exactly two weeks left to submit your abstract to the EMBO Mobile Genome Workshop.
☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th
☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th
Reposted by Sven Klumpe
Learn more about the collaboration possibilities offered at Vienna BioCenter as Victoria Deneke shares her insights as a Postdoc at the Research Institute of Molecular Pathology.
The VIP³ PostDoc Program call is open – apply now! training.vbc.ac.at/post-docs/vi...
#VBCPostDoc
The VIP³ PostDoc Program call is open – apply now! training.vbc.ac.at/post-docs/vi...
#VBCPostDoc
June 11, 2025 at 8:52 AM
Learn more about the collaboration possibilities offered at Vienna BioCenter as Victoria Deneke shares her insights as a Postdoc at the Research Institute of Molecular Pathology.
The VIP³ PostDoc Program call is open – apply now! training.vbc.ac.at/post-docs/vi...
#VBCPostDoc
The VIP³ PostDoc Program call is open – apply now! training.vbc.ac.at/post-docs/vi...
#VBCPostDoc
Reposted by Sven Klumpe
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
May 26, 2025 at 4:56 PM
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
Reposted by Sven Klumpe
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 6, 2025 at 4:27 PM
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Reposted by Sven Klumpe
Our work on HIV-1 matrix, led by James Stacey and @dhrebik.bsky.social, is in this week's @nature.com. Extended explanatory video from @margotriggi.bsky.social.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
April 4, 2025 at 1:03 PM
Our work on HIV-1 matrix, led by James Stacey and @dhrebik.bsky.social, is in this week's @nature.com. Extended explanatory video from @margotriggi.bsky.social.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...