Sven Klumpe
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svenklumpe.bsky.social
Sven Klumpe
@svenklumpe.bsky.social
CryoFIB Development and In situ structural biology. NPC and TE enthusiast. Drosophilist but ready to switch to wherever biology takes us

Getting to enjoy science with a group at IMBA/IMP of the Vienna BioCenter https://www.imp.ac.at/groups/sven-klumpe
In order to optimize parameters for such a probe, we used a spot burn that we image with the shaped probe in order to get an estimate of the beam shape (e.g. how the video in the first post is produced). We can then image the full ion knife shape using spot burns in silicon and FIBSEM volume imaging
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM