Javi
@structjavi.bsky.social
Post-doc at the @wilflinglab.bsky.social in @mpibp.bsky.social
Previously EMBL Hamburg
#StructuralBiology under liquid Nitrogen #cryoEM / #cryoET
Mountain-lover
He/him
Previously EMBL Hamburg
#StructuralBiology under liquid Nitrogen #cryoEM / #cryoET
Mountain-lover
He/him
Reposted by Javi
Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁♀️💎✨VIP section💅🪩🥂
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Prohibitin complexes associate with unique membrane microdomains in cells https://www.biorxiv.org/content/10.1101/2025.11.14.688579v1
November 17, 2025 at 12:22 AM
Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁♀️💎✨VIP section💅🪩🥂
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Reposted by Javi
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
November 6, 2025 at 7:38 AM
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Now that it’s November and Halloween is over, is it okay to put up the Christmas tree yet?
November 5, 2025 at 7:58 PM
Now that it’s November and Halloween is over, is it okay to put up the Christmas tree yet?
Reposted by Javi
Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts!
🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
📍 Frankfurt | 📆 April 12–20, 2026
🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
📍 Frankfurt | 📆 April 12–20, 2026
October 23, 2025 at 9:10 AM
Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts!
🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
📍 Frankfurt | 📆 April 12–20, 2026
🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
📍 Frankfurt | 📆 April 12–20, 2026
Reposted by Javi
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
October 15, 2025 at 6:26 AM
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
Reposted by Javi
Are you ready to lead ground-breaking research in Infection Biochemistry?
We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
September 17, 2025 at 8:32 AM
Are you ready to lead ground-breaking research in Infection Biochemistry?
We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
Reposted by Javi
Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de
t1p.de/cxt7a
t1p.de/cxt7a
September 4, 2025 at 8:03 AM
Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de
t1p.de/cxt7a
t1p.de/cxt7a
Reposted by Javi
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Reposted by Javi
More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, Tomáš Majtner, Beata Turoňová, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...
Optimal tilt-increment for cryo-ET
Cryo-electron tomography (cryo-ET) enables high-resolution, three-dimensional imaging of cellular structures in their native, frozen state. However, image quality is limited by a trade-off between ang...
www.biorxiv.org
August 23, 2025 at 9:18 AM
More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, Tomáš Majtner, Beata Turoňová, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...
Reposted by Javi
We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
August 18, 2025 at 11:29 AM
We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
New luminescent animal unlocked!
July's best science images, as chosen by the Nature photo team, include a glow-in-the-dark marsupial showing off its luminous furr, which earned photographer Ben Alldridge a spot as a finalist in the Beaker Street Science Photography Prize 2025. See more photos at the link. #SciArt 🧪 🔭 🏺
Glow-in-the-dark marsupial shows off its luminous fur — July’s best science images
The month’s sharpest science shots, selected by Nature’s photo team.
go.nature.com
August 6, 2025 at 2:02 PM
New luminescent animal unlocked!
Reposted by Javi
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
July 12, 2025 at 12:49 AM
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
Reposted by Javi
Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.
Exclusive: Famed protein structure competition nears end as NIH grant money runs out
Agency silent on funding renewal for contest that inspired creation of AIs that predicted how proteins would fold
www.science.org
July 4, 2025 at 12:07 PM
Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.
Reposted by Javi
This is Ana’s third ERC grant! We’re proud to celebrate her success and groundbreaking work here at the Max Planck Institute of Biophysics. #ERCAdG Read more here 🔗 www.biophys.mpg.de/2872321/garcia-saez-awarded-erc-grant
Ana García-Sáez Awarded Prestigious ERC Grant
www.biophys.mpg.de
June 17, 2025 at 12:17 PM
This is Ana’s third ERC grant! We’re proud to celebrate her success and groundbreaking work here at the Max Planck Institute of Biophysics. #ERCAdG Read more here 🔗 www.biophys.mpg.de/2872321/garcia-saez-awarded-erc-grant
Reposted by Javi
Our latest research is out 📄 !
This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.
@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social
This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.
@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social
May 12, 2025 at 8:15 AM
Our latest research is out 📄 !
This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.
@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social
This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.
@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social
Reposted by Javi
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
April 23, 2025 at 8:49 AM
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Reposted by Javi
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Look at those nucleosomes! 🧬 Impressive work on chromatin architecture from our colleagues, check it out below! ⬇️
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
April 11, 2025 at 1:01 PM
Look at those nucleosomes! 🧬 Impressive work on chromatin architecture from our colleagues, check it out below! ⬇️
Reposted by Javi
Swinging lever mechanism of myosin shown by time-resolved cryoEM www.nature.com/articles/s41... so glad to finally be able to share this with the community! @astburycentre.bsky.social @astbury-bsl.bsky.social @stemuench.bsky.social @snmcmillan.bsky.social @davidklebl.bsky.social
Swinging lever mechanism of myosin directly shown by time-resolved cryo-EM - Nature
A study using time-resolved cryogenic electron microscopy reveals the swinging lever mechanism of myosin, providing information on the molecular basis behind the production of force and movement by my...
www.nature.com
April 9, 2025 at 5:19 PM
Swinging lever mechanism of myosin shown by time-resolved cryoEM www.nature.com/articles/s41... so glad to finally be able to share this with the community! @astburycentre.bsky.social @astbury-bsl.bsky.social @stemuench.bsky.social @snmcmillan.bsky.social @davidklebl.bsky.social
Reposted by Javi
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
Reposted by Javi
Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:
February 6, 2025 at 11:02 AM
Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:
Reposted by Javi
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:20 PM
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
Interesting sample preparation method moving away from blotting
Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1
March 12, 2025 at 5:56 PM
Interesting sample preparation method moving away from blotting
Reposted by Javi
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
March 5, 2025 at 4:02 PM
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
I will think of this paper next time I am there... 😀
Nature research paper: Emergence of collective oscillations in massive human crowds
https://go.nature.com/414Ay28
https://go.nature.com/414Ay28
Emergence of collective oscillations in massive human crowds - Nature
Analysis of the confined crowds at the San Fermín festival in Spain shows that dense crowds can self-organize into macroscopic chiral oscillators, coordinating the orbital motion of hundreds of individuals without external guidance.
go.nature.com
February 7, 2025 at 10:44 AM
I will think of this paper next time I am there... 😀