Sadie Antine
@sadieantine.bsky.social
PhD in Virology Harvard Kranzusch Lab Alum
Reposted by Sadie Antine
Bacteria can sense when a virus starts shredding their genome — by detecting methylated mononucleotides.
Here’s the story of how we discovered the Metis defense system 👇
www.biorxiv.org/content/10.1...
Here’s the story of how we discovered the Metis defense system 👇
www.biorxiv.org/content/10.1...
November 6, 2025 at 5:00 AM
Bacteria can sense when a virus starts shredding their genome — by detecting methylated mononucleotides.
Here’s the story of how we discovered the Metis defense system 👇
www.biorxiv.org/content/10.1...
Here’s the story of how we discovered the Metis defense system 👇
www.biorxiv.org/content/10.1...
Reposted by Sadie Antine
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
October 24, 2025 at 8:11 AM
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
Reposted by Sadie Antine
🚨 Systems Virology Journal Club – 8th Series! 🚨
#BillSchneider and I are delighted to announce another round of cutting-edge talks in #SystemsVirology! 🦠💡
Join us and an outstanding lineup of speakers, starting Oct 30.
Free registration: shiraweingartengabbay.com/systems-viro... 🔬✨
#BillSchneider and I are delighted to announce another round of cutting-edge talks in #SystemsVirology! 🦠💡
Join us and an outstanding lineup of speakers, starting Oct 30.
Free registration: shiraweingartengabbay.com/systems-viro... 🔬✨
October 20, 2025 at 2:55 PM
🚨 Systems Virology Journal Club – 8th Series! 🚨
#BillSchneider and I are delighted to announce another round of cutting-edge talks in #SystemsVirology! 🦠💡
Join us and an outstanding lineup of speakers, starting Oct 30.
Free registration: shiraweingartengabbay.com/systems-viro... 🔬✨
#BillSchneider and I are delighted to announce another round of cutting-edge talks in #SystemsVirology! 🦠💡
Join us and an outstanding lineup of speakers, starting Oct 30.
Free registration: shiraweingartengabbay.com/systems-viro... 🔬✨
Reposted by Sadie Antine
Thank you to the Blavatnik Family Foundation and The New York Academy of Sciences for honoring our research uniting human innate immunity and bacterial anti-phage defense at @danafarber.bsky.social @harvardmicro.bsky.social @harvard.edu
bit.ly/4pZUDkF
blavatnikawards.org/news/items/t...
bit.ly/4pZUDkF
blavatnikawards.org/news/items/t...
October 9, 2025 at 4:44 PM
Thank you to the Blavatnik Family Foundation and The New York Academy of Sciences for honoring our research uniting human innate immunity and bacterial anti-phage defense at @danafarber.bsky.social @harvardmicro.bsky.social @harvard.edu
bit.ly/4pZUDkF
blavatnikawards.org/news/items/t...
bit.ly/4pZUDkF
blavatnikawards.org/news/items/t...
Reposted by Sadie Antine
Our story describing the Panoptes bacterial immune defense system is now finally peer-reviewed and published today! www.nature.com/articles/s41...
The Panoptes system uses decoy cyclic nucleotides to defend against phage - Nature
The Panoptes antiphage system defends bacteria by detecting phage-encoded counter-defences that sequester cyclic nucleotide signals, triggering membrane disruption and highlighting a broader strategy of sensing immune evasion through second-messenger surveillance.
www.nature.com
October 1, 2025 at 4:05 PM
Our story describing the Panoptes bacterial immune defense system is now finally peer-reviewed and published today! www.nature.com/articles/s41...
Reposted by Sadie Antine
Are viruses capable of regulating protein synthesis in the nuanced way of cellular organisms? Kinda! I’m excited to share some of my postdoc work that leveraged giant DNA viruses to address this question.
Giant DNA viruses encode a hallmark translation initiation complex of eukaryotic life
In contrast to living organisms, viruses were long thought to lack protein synthesis machinery and instead depend on host factors to translate viral transcripts. Here, we discover that giant DNA virus...
www.biorxiv.org
October 2, 2025 at 12:32 AM
Are viruses capable of regulating protein synthesis in the nuanced way of cellular organisms? Kinda! I’m excited to share some of my postdoc work that leveraged giant DNA viruses to address this question.
Reposted by Sadie Antine
Huntingtin binds and bundles F-actin!
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
September 25, 2025 at 3:01 PM
Huntingtin binds and bundles F-actin!
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Reposted by Sadie Antine
You’ve heard of ubiquitination, meet deazaguanylation: Doug Wassarman in our lab discovered phage defense pathways have co-opted Q nucleobase biosynthetic enzymes to catalyze a new form of protein conjugation chemistry @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
September 25, 2025 at 7:16 PM
You’ve heard of ubiquitination, meet deazaguanylation: Doug Wassarman in our lab discovered phage defense pathways have co-opted Q nucleobase biosynthetic enzymes to catalyze a new form of protein conjugation chemistry @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sadie Antine
Very happy to share that I will be starting my lab at AITHYRA in October! My lab will use structural bioinformatics and functional genomics to understand the function of viral proteins, with a special emphasis on understanding how viruses subvert innate immunity.
Welcome JASON NOMBURG! With great pleasure we announce that Jason Nomburg is one of the Starting Principal Investigators LS who will join AITHYRA in October 2025. Jason wants to understand the function of proteins in the virome.
More information: www.oeaw.ac.at/aithyra/rese... #AITHYRA #StartingPI
More information: www.oeaw.ac.at/aithyra/rese... #AITHYRA #StartingPI
August 15, 2025 at 7:54 PM
Very happy to share that I will be starting my lab at AITHYRA in October! My lab will use structural bioinformatics and functional genomics to understand the function of viral proteins, with a special emphasis on understanding how viruses subvert innate immunity.
Reposted by Sadie Antine
How can we understand the earliest events in evolution of eukaryotic immunity? @yao-li.bsky.social reports incredible molecular fossils of complete bacterial-like operons in eukaryotes that illuminate how animal immunity was first acquired from anti-phage defense
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
September 5, 2025 at 10:13 AM
How can we understand the earliest events in evolution of eukaryotic immunity? @yao-li.bsky.social reports incredible molecular fossils of complete bacterial-like operons in eukaryotes that illuminate how animal immunity was first acquired from anti-phage defense
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sadie Antine
Excited to share our new @natmicrobiol.nature.com paper revealing the most complete arenavirus glycoprotein complex (GPC) structures to date 🤩 www.nature.com/articles/s41...
Molecular organization of the New World arenavirus spike glycoprotein complex - Nature Microbiology
Cryo-EM structures of the full-length Junin virus and Machupo virus spike glycoprotein complexes stabilized in the prefusion conformation. Analyses reveal features that regulate glycoprotein pH-depend...
www.nature.com
August 9, 2025 at 9:51 AM
Excited to share our new @natmicrobiol.nature.com paper revealing the most complete arenavirus glycoprotein complex (GPC) structures to date 🤩 www.nature.com/articles/s41...
Reposted by Sadie Antine
First preprint from the Nemudryi Lab! 🍾
In this work, we link antiviral immunity in bacteria and humans by showing that homologs of human Schlafen nucleases protect bacteria from phages.
www.biorxiv.org/content/10.1...
In this work, we link antiviral immunity in bacteria and humans by showing that homologs of human Schlafen nucleases protect bacteria from phages.
www.biorxiv.org/content/10.1...
Bacterial Schlafens mediate anti-phage defense
Human Schlafen proteins restrict viral replication by cleaving tRNA, thereby suppressing protein synthesis. Although the ribonuclease domain of Schlafen proteins is conserved across all domains of lif...
www.biorxiv.org
July 25, 2025 at 2:52 PM
First preprint from the Nemudryi Lab! 🍾
In this work, we link antiviral immunity in bacteria and humans by showing that homologs of human Schlafen nucleases protect bacteria from phages.
www.biorxiv.org/content/10.1...
In this work, we link antiviral immunity in bacteria and humans by showing that homologs of human Schlafen nucleases protect bacteria from phages.
www.biorxiv.org/content/10.1...
Reposted by Sadie Antine
Many components of human immunity evolved from ancient pathways in bacteria. A beautiful story from @audeber.bsky.social @enzopoirier.bsky.social leverages this evolutionary connection to discover SIRal as a signaling protein in animal TLR-mediated innate immunity.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
A human homolog of SIR2 antiphage proteins mediates immunity via the Toll-like receptor pathway
Key actors of mammalian immunity originated from bacterial antiphage systems. The full extent of immune system conservation between bacteria and eukaryotes is unknown. Here, we show that the silent in...
www.science.org
July 24, 2025 at 6:22 PM
Many components of human immunity evolved from ancient pathways in bacteria. A beautiful story from @audeber.bsky.social @enzopoirier.bsky.social leverages this evolutionary connection to discover SIRal as a signaling protein in animal TLR-mediated innate immunity.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sadie Antine
A new preprint led by Sonomi Yamaguchi in our lab describes a bacterial anti-phage defense system named Clover that uses nucleotide signals to both activate and inhibit host immunity.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
July 17, 2025 at 8:38 PM
A new preprint led by Sonomi Yamaguchi in our lab describes a bacterial anti-phage defense system named Clover that uses nucleotide signals to both activate and inhibit host immunity.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Welcome to the biochemical world of anti-phage nuclease-NTPases!🤩🤩
Starting the lab Bluesky account to share a preprint from @aragucci.bsky.social and @sadieantine.bsky.social that reveals molecular principles shared across diverse nuclease-NTPase anti-phage defense systems in bacterial immunity (1/7)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Nuclease-NTPase systems use shared molecular features to control bacterial anti-phage defense
Bacteria encode an enormous diversity of defense systems including restriction-modification and CRISPR-Cas that cleave nucleic acid to protect against phage infection. Bioinformatic analyses demonstra...
www.biorxiv.org
July 17, 2025 at 4:46 PM
Welcome to the biochemical world of anti-phage nuclease-NTPases!🤩🤩
Reposted by Sadie Antine
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
April 30, 2025 at 6:44 PM
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
DNA-gated ion channel🤯 Congrats Joel!!
Excited to share my PhD work in the Kranzusch Lab published in @nature.com!
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
A DNA-gated molecular guard controls bacterial Hailong anti-phage defence - Nature
Nature - A DNA-gated molecular guard controls bacterial Hailong anti-phage defence
www.nature.com
May 1, 2025 at 1:12 AM
DNA-gated ion channel🤯 Congrats Joel!!
Reposted by Sadie Antine
Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
April 25, 2025 at 4:15 PM
Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Reposted by Sadie Antine
🧪Exciting news out today in the world of Mendelian genetics (a statement for a niche crowd if ever there was one). Read my piece at @nature.com if, like me, you are surprised to hear we hadn't figured this out a long time ago.
www.nature.com/articles/d41...
www.nature.com/articles/d41...
Century-old genetics mystery of Mendel’s peas finally solved
Researchers pinpoint the genes responsible for the final three pea traits studied by the famed citizen scientist.
www.nature.com
April 23, 2025 at 4:35 PM
🧪Exciting news out today in the world of Mendelian genetics (a statement for a niche crowd if ever there was one). Read my piece at @nature.com if, like me, you are surprised to hear we hadn't figured this out a long time ago.
www.nature.com/articles/d41...
www.nature.com/articles/d41...
Reposted by Sadie Antine
It was an honor for my lab and myself to host the meeting on the Immune Systems of Bacteria. Such a high from all the incredible science and sharing with scientists from the whole world the greatness of Paris in the spring.
Vive la science, et vive Paris!
Vive la science, et vive Paris!
April 11, 2025 at 3:46 PM
It was an honor for my lab and myself to host the meeting on the Immune Systems of Bacteria. Such a high from all the incredible science and sharing with scientists from the whole world the greatness of Paris in the spring.
Vive la science, et vive Paris!
Vive la science, et vive Paris!
Reposted by Sadie Antine
I’m excited to share my first paper in the Farnung lab, in which we report four cryo-EM structures of the human DNA replication machinery engaging with and progressing into a nucleosome.
🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome
April 6, 2025 at 10:43 AM
I’m excited to share my first paper in the Farnung lab, in which we report four cryo-EM structures of the human DNA replication machinery engaging with and progressing into a nucleosome.
Check out Doug’s awesome work!!
Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity https://www.biorxiv.org/content/10.1101/2025.04.06.647259v1
April 7, 2025 at 8:39 AM
Check out Doug’s awesome work!!
Reposted by Sadie Antine
1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
March 17, 2025 at 11:56 AM
1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯
Our new study reveals how retrotransposons invade the germline directly from somatic cells.
www.biorxiv.org/content/10.1...
A short thread 🧵👇
Reposted by Sadie Antine
Foldseek-Multimer—now published in @naturemethods.bsky.social—our fast multimer search tool, enables complex comparisons against the full PDB in seconds. It comes with BFMD, a collection of 300K+ predictions gathered from community projects.
📄 nature.com/articles/s41...
🌐 search.foldseek.com
📄 nature.com/articles/s41...
🌐 search.foldseek.com
February 6, 2025 at 8:25 AM
Foldseek-Multimer—now published in @naturemethods.bsky.social—our fast multimer search tool, enables complex comparisons against the full PDB in seconds. It comes with BFMD, a collection of 300K+ predictions gathered from community projects.
📄 nature.com/articles/s41...
🌐 search.foldseek.com
📄 nature.com/articles/s41...
🌐 search.foldseek.com
Reposted by Sadie Antine
🚨 🧬 Updated preprint on the completed BASEL collection - what's new? Some fun with bacterial immunity and a new P22 relative infecting E. coli K-12. A thread 🧵
February 6, 2025 at 6:05 AM
🚨 🧬 Updated preprint on the completed BASEL collection - what's new? Some fun with bacterial immunity and a new P22 relative infecting E. coli K-12. A thread 🧵