Chlanda lab
@chlandalab.bsky.social
Membrane Biology of Viral Infection
cryo-ET, cryo-CLEM, cryo-FIB, influenza, Ebola virus, SARS-CoV-2
cryo-ET, cryo-CLEM, cryo-FIB, influenza, Ebola virus, SARS-CoV-2
Reposted by Chlanda lab
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
November 4, 2025 at 5:20 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted by Chlanda lab
Our new preprint is out 🥳🥳🥳
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
November 3, 2025 at 12:26 PM
Our new preprint is out 🥳🥳🥳
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Reposted by Chlanda lab
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
October 29, 2025 at 5:11 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted by Chlanda lab
warwick.ac.uk/fac/cross_fa...
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
Dr Jeremy Keown
Dr Jeremy Keown
warwick.ac.uk
October 24, 2025 at 7:58 AM
warwick.ac.uk/fac/cross_fa...
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
October 24, 2025 at 8:11 AM
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
Reposted by Chlanda lab
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
October 16, 2025 at 7:03 PM
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...
Reposted by Chlanda lab
Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...
Link: www.biorxiv.org/content/10.1...
September 19, 2025 at 6:07 PM
Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...
Link: www.biorxiv.org/content/10.1...
Reposted by Chlanda lab
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026
September 23, 2025 at 10:42 AM
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026
Reposted by Chlanda lab
Since it was published at peak holiday season, I'm taking the liberty to re-introduce our recent review article. We hope it will be a useful overview of RNA virus replication strategies, and how in situ cryo-EM have helped us understand them:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Illuminating druggable dark matter in RNA virus replication using in situ cryo-EM
Viral proteins typically exist in the context of complex virions or in the even more complex host cells in which they replicate. Hence, meaningful ins…
www.sciencedirect.com
September 22, 2025 at 9:26 AM
Since it was published at peak holiday season, I'm taking the liberty to re-introduce our recent review article. We hope it will be a useful overview of RNA virus replication strategies, and how in situ cryo-EM have helped us understand them:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Chlanda lab
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Reposted by Chlanda lab
One week left to apply for a fully funded PhD position in my group! Please repost. jobrxiv.org/job/max-plan...
PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE
Post a job in 3min, or find thousands of job offers like this one at jobRxiv!
jobrxiv.org
August 21, 2025 at 9:02 AM
One week left to apply for a fully funded PhD position in my group! Please repost. jobrxiv.org/job/max-plan...
Reposted by Chlanda lab
Awesome malaria study out now in Nature Microbiology:
An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission
By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues!
@freddyfrischknecht.bsky.social
www.nature.com/articles/s41...
An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission
By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues!
@freddyfrischknecht.bsky.social
www.nature.com/articles/s41...
An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission - Nature Microbiology
A unique Arp2/3 complex in malaria parasites enables DNA segregation during the rapid formation of male gametes by safeguarding the attachment of chromosomes to the mitotic spindle.
www.nature.com
June 13, 2025 at 10:31 AM
Awesome malaria study out now in Nature Microbiology:
An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission
By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues!
@freddyfrischknecht.bsky.social
www.nature.com/articles/s41...
An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission
By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues!
@freddyfrischknecht.bsky.social
www.nature.com/articles/s41...
Reposted by Chlanda lab
Really enjoyed writing about one of my favorite topics (influenza filaments). There is so much exciting new filament research now, it is clearly no longer 'the graveyard of postdocs' Bob Lamb called the topic during my PhD. www.embopress.org/doi/full/10....
Influenza filaments: a graveyard of postdocs no longer? | The EMBO Journal
EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.
www.embopress.org
June 10, 2025 at 7:19 PM
Really enjoyed writing about one of my favorite topics (influenza filaments). There is so much exciting new filament research now, it is clearly no longer 'the graveyard of postdocs' Bob Lamb called the topic during my PhD. www.embopress.org/doi/full/10....
Very nice News&Views summary written by
@brucelab.bsky.social about our work and work by Ed Partlow et al. (NIH) that influenza A virus filamentous morphology plays an important role in infection (www.nature.com/articles/s41...) can.
Thank you!
(www.embopress.org/doi/full/10....)
@brucelab.bsky.social about our work and work by Ed Partlow et al. (NIH) that influenza A virus filamentous morphology plays an important role in infection (www.nature.com/articles/s41...) can.
Thank you!
(www.embopress.org/doi/full/10....)
June 11, 2025 at 12:00 PM
Very nice News&Views summary written by
@brucelab.bsky.social about our work and work by Ed Partlow et al. (NIH) that influenza A virus filamentous morphology plays an important role in infection (www.nature.com/articles/s41...) can.
Thank you!
(www.embopress.org/doi/full/10....)
@brucelab.bsky.social about our work and work by Ed Partlow et al. (NIH) that influenza A virus filamentous morphology plays an important role in infection (www.nature.com/articles/s41...) can.
Thank you!
(www.embopress.org/doi/full/10....)
We are happy this work by Sarah Peterl et al. is out in EMBO J! It was an exciting collaborative effort of several groups in the SFB1129 consortium on pathogen replication and spread. Check out the correlated light and scanning EM method to study virus morphology changes during spread!
June 11, 2025 at 11:42 AM
We are happy this work by Sarah Peterl et al. is out in EMBO J! It was an exciting collaborative effort of several groups in the SFB1129 consortium on pathogen replication and spread. Check out the correlated light and scanning EM method to study virus morphology changes during spread!
Reposted by Chlanda lab
Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis
Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...
www.science.org
May 15, 2025 at 7:51 PM
Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Chlanda lab
Please RT. Post-doc opportunity alert! 💥 closing 5th May.. Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
April 28, 2025 at 4:41 PM
Please RT. Post-doc opportunity alert! 💥 closing 5th May.. Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted by Chlanda lab
Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
April 25, 2025 at 10:25 AM
Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!
Reposted by Chlanda lab
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
April 23, 2025 at 8:49 AM
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Reposted by Chlanda lab
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
April 11, 2025 at 9:04 AM
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
Reposted by Chlanda lab
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
Reposted by Chlanda lab
I’m delighted that we could contribute the #cryoEM data to this incredible antiviral discovery project from the group of @neytsjohan.bsky.social 💊
"A coronavirus assembly inhibitor that targets the viral membrane protein"
www.nature.com/articles/s41...
A huge congrats to everyone involved! 🎉
"A coronavirus assembly inhibitor that targets the viral membrane protein"
www.nature.com/articles/s41...
A huge congrats to everyone involved! 🎉
March 26, 2025 at 4:31 PM
I’m delighted that we could contribute the #cryoEM data to this incredible antiviral discovery project from the group of @neytsjohan.bsky.social 💊
"A coronavirus assembly inhibitor that targets the viral membrane protein"
www.nature.com/articles/s41...
A huge congrats to everyone involved! 🎉
"A coronavirus assembly inhibitor that targets the viral membrane protein"
www.nature.com/articles/s41...
A huge congrats to everyone involved! 🎉
Reposted by Chlanda lab
Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1
March 15, 2025 at 7:17 AM
Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1
This is a great opportunity to establish a virology research group at CIID, Heidelberg University!
Applications are open for new Schaller Research Group Leader Positions in the field of Infectious Diseases at Heidelberg University. These generously funded positions of 5+2 years are a great opportunity for young virologists!
Find out more here:
www.klinikum.uni-heidelberg.de/fileadmin/in...
Find out more here:
www.klinikum.uni-heidelberg.de/fileadmin/in...
March 5, 2025 at 12:56 PM
This is a great opportunity to establish a virology research group at CIID, Heidelberg University!