Reposted
1/ 🧵 I am excited to share a new preprint from our group:
Single-cell Dam&T-seq during mouse corticogenesis reveals how genome–lamina interactions regulate long neuronal genes.
doi.org/10.1101/2025...
#Neurodevelopment #Epigenetics @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social
Single-cell Dam&T-seq during mouse corticogenesis reveals how genome–lamina interactions regulate long neuronal genes.
doi.org/10.1101/2025...
#Neurodevelopment #Epigenetics @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social
MeCP2 binding and genome–lamina reorganization precede long gene activation during mouse corticogenesis
During corticogenesis, neural gene expression is tightly coordinated by chromatin and epigenetic changes, whose misregulation can lead to neurodevelopmental disorders[1][1]–[4][2]. The role of spatial...
doi.org
September 17, 2025 at 8:41 AM
1/ 🧵 I am excited to share a new preprint from our group:
Single-cell Dam&T-seq during mouse corticogenesis reveals how genome–lamina interactions regulate long neuronal genes.
doi.org/10.1101/2025...
#Neurodevelopment #Epigenetics @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social
Single-cell Dam&T-seq during mouse corticogenesis reveals how genome–lamina interactions regulate long neuronal genes.
doi.org/10.1101/2025...
#Neurodevelopment #Epigenetics @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social
Reposted
Agreed, truly a team effort - but a huge amount of credit to @kataruka.bsky.social for her leadership. Smarca5 is important for chromatin remodeling in DNA damage repair during male meiosis (among other things!)
Truly a team effort from the Lesch lab @ambystoma22.bsky.social Happy to share this paper where we study the role of Smarca5 in spermatogenesis.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
SMARCA5 restricts chromatin accessibility to promote male meiosis and fertility in mammals | PNAS
Establishment of correct chromatin configuration in male meiosis is essential for
sperm formation and male fertility. However, how chromatin remode...
www.pnas.org
August 2, 2025 at 4:17 AM
Agreed, truly a team effort - but a huge amount of credit to @kataruka.bsky.social for her leadership. Smarca5 is important for chromatin remodeling in DNA damage repair during male meiosis (among other things!)
Reposted
Our paper is now out in Molecular Cell!
Check the thread in this former post:
www.cell.com/molecular-ce...
Check the thread in this former post:
www.cell.com/molecular-ce...
July 17, 2025 at 5:41 PM
Our paper is now out in Molecular Cell!
Check the thread in this former post:
www.cell.com/molecular-ce...
Check the thread in this former post:
www.cell.com/molecular-ce...
Reposted
ICYMI: New Online! Degrons: defining the rules of protein degradation
Degrons: defining the rules of protein degradation
Nature Reviews Molecular Cell Biology, Published online: 14 July 2025; doi:10.1038/s41580-025-00870-zDegrons allow E3 ubiquitin ligases to identify their substrates, and thus have a central role in protein degradation by the ubiquitin–proteasome system. This Review discusses the latest insights into the mechanisms underlying degron function, the relevance of degrons in disease and how degrons can be harnessed for therapeutic protein degradation.
bit.ly
July 17, 2025 at 3:36 PM
ICYMI: New Online! Degrons: defining the rules of protein degradation
Reposted
Very excited to share our new work on gastruloids by the incredible Cat Triandafillou! We mapped gene expression across 26 individual gastruloids at single-cell resolution and discovered some pretty amazing patterns about how these "mini-embryos" organize themselves.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Single-cell spatial mapping reveals reproducible cell type organization and spatially-dependent gene expression in gastruloids
Gastruloids are three-dimensional stem-cell-based models that recapitulate key aspects of mammalian gastrulation, including formation of an anterior-posterior (AP) axis. However, we do not have detail...
www.biorxiv.org
July 15, 2025 at 3:43 PM
Very excited to share our new work on gastruloids by the incredible Cat Triandafillou! We mapped gene expression across 26 individual gastruloids at single-cell resolution and discovered some pretty amazing patterns about how these "mini-embryos" organize themselves.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted
Liquid-liquid phase separation of HP1? Not in our mouse fibroblasts… This paper by the Fabian Erdel group offers a fresh perspective on how this can be reconciled with seemingly conflicting reports: The phase separation propensity of HP1 decreases from yeast → fly → mouse. doi.org/10.1038/s414...
July 11, 2025 at 5:35 AM
Liquid-liquid phase separation of HP1? Not in our mouse fibroblasts… This paper by the Fabian Erdel group offers a fresh perspective on how this can be reconciled with seemingly conflicting reports: The phase separation propensity of HP1 decreases from yeast → fly → mouse. doi.org/10.1038/s414...
Reposted
I am very excited to share our latest work where we describe a new method to profile genome-wide chromatin transitions over time in single cells. Great collaborative effort with the van Oudenaarden group @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social www.nature.com/articles/s41....
Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation - Nature Cell Biology
Kefalopoulou, Rullens et al. develop Dam&ChIC to assay chromatin state at two different time points in the same cell. The method was used to study the reorganization of LADs during cell division a...
www.nature.com
July 10, 2025 at 2:36 PM
I am very excited to share our latest work where we describe a new method to profile genome-wide chromatin transitions over time in single cells. Great collaborative effort with the van Oudenaarden group @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social www.nature.com/articles/s41....
Reposted
Nature research paper: Maternal iron deficiency causes male-to-female sex reversal in mouse embryos
https://go.nature.com/45fG1G1
https://go.nature.com/45fG1G1
Maternal iron deficiency causes male-to-female sex reversal in mouse embryos - Nature
Iron-deficient conditions in pregnant mice can cause XY mouse embryos to develop female rather than male genitalia, revealing that iron metabolism has a role in determining male sex in mice.
go.nature.com
June 5, 2025 at 11:48 AM
Nature research paper: Maternal iron deficiency causes male-to-female sex reversal in mouse embryos
https://go.nature.com/45fG1G1
https://go.nature.com/45fG1G1
Reposted
Very excited to share our latest work in #MolCell showing NPM1 stabilizing the association of nucleolus associated domains (NADs) and recruiting G9a to establish their repressive chromatin states. www.sciencedirect.com/science/arti...
May 24, 2025 at 11:12 AM
Very excited to share our latest work in #MolCell showing NPM1 stabilizing the association of nucleolus associated domains (NADs) and recruiting G9a to establish their repressive chromatin states. www.sciencedirect.com/science/arti...