@oleuns.bsky.social
Reposted
We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...
Downloads – CCP-EM
www.ccpem.ac.uk
November 4, 2025 at 5:38 PM
We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...
Reposted
Image of the Sun.
Taken at night.
Not looking up at sky but down through 8000 miles of rock to the other side of the Earth.
Not with light but neutrinos.
Taken at night.
Not looking up at sky but down through 8000 miles of rock to the other side of the Earth.
Not with light but neutrinos.
November 1, 2025 at 7:50 AM
Image of the Sun.
Taken at night.
Not looking up at sky but down through 8000 miles of rock to the other side of the Earth.
Not with light but neutrinos.
Taken at night.
Not looking up at sky but down through 8000 miles of rock to the other side of the Earth.
Not with light but neutrinos.
Reposted
my friend and wonderful colleague Stan is hiring at Genentech in South San Francisco! 🧬 🌉
Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility
www.linkedin.com/posts/stanis...
Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility
www.linkedin.com/posts/stanis...
#hiring #researchassociate #structuralbiology #proteinsciences #cryoem #biochemistry #biophysics #dnarepair #drugdiscovery | Stanislau Yatskevich
We’re hiring a 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐀𝐬𝐬𝐨𝐜𝐢𝐚𝐭𝐞 (Contractor) to join our 𝐒𝐭𝐫𝐮𝐜𝐭𝐮𝐫𝐚𝐥 𝐁𝐢𝐨𝐥𝐨𝐠𝐲 team in 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 𝐒𝐜𝐢𝐞𝐧𝐜𝐞𝐬 focused on the molecular mechanisms of DNA repair.
𝐖𝐡𝐲 𝐭𝐡𝐢𝐬 𝐫𝐨𝐥𝐞 𝐦𝐚𝐭𝐭𝐞𝐫𝐬
You’ll help purify and ...
www.linkedin.com
October 31, 2025 at 9:05 PM
my friend and wonderful colleague Stan is hiring at Genentech in South San Francisco! 🧬 🌉
Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility
www.linkedin.com/posts/stanis...
Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility
www.linkedin.com/posts/stanis...
Reposted
EMBL scientists have new detailed info about Asgard archaea DNA structures offering important clues to more complex cell evolution.
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
October 29, 2025 at 8:29 AM
EMBL scientists have new detailed info about Asgard archaea DNA structures offering important clues to more complex cell evolution.
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
Reposted
We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated.
mrc.tal.net/vx/appcentre...
mrc.tal.net/vx/appcentre...
Quick Check Needed
mrc.tal.net
October 29, 2025 at 9:17 AM
We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated.
mrc.tal.net/vx/appcentre...
mrc.tal.net/vx/appcentre...
Reposted
The German company that makes the mechanical ladder used in the Louvre heist has used the image to advertise, with the text 'When you need to move fast'
10/10 response, no notes
10/10 response, no notes
October 24, 2025 at 8:27 AM
The German company that makes the mechanical ladder used in the Louvre heist has used the image to advertise, with the text 'When you need to move fast'
10/10 response, no notes
10/10 response, no notes
Reposted
Finally, just about 2 years after finishing the postdoc the last part of my work is finally online! We determined the structure of HSV-1 Origin Binding Protein, the viral initiator of DNA replication, by cryo-EM 🧪🧬🧶🧵 #cryoEM #structuralbiology
October 23, 2025 at 6:37 AM
Finally, just about 2 years after finishing the postdoc the last part of my work is finally online! We determined the structure of HSV-1 Origin Binding Protein, the viral initiator of DNA replication, by cryo-EM 🧪🧬🧶🧵 #cryoEM #structuralbiology
Reposted
Using cryo-EM, we determined OBP structures at up to 2.8 Å resolution in multiple states:
1. bound to HSV-1 origin of replication (OriS) DNA
2. with a non-hydrolyzable ATP analog
3. as monomer, dimer, and higher-order assemblies
1. bound to HSV-1 origin of replication (OriS) DNA
2. with a non-hydrolyzable ATP analog
3. as monomer, dimer, and higher-order assemblies
October 23, 2025 at 6:40 AM
Using cryo-EM, we determined OBP structures at up to 2.8 Å resolution in multiple states:
1. bound to HSV-1 origin of replication (OriS) DNA
2. with a non-hydrolyzable ATP analog
3. as monomer, dimer, and higher-order assemblies
1. bound to HSV-1 origin of replication (OriS) DNA
2. with a non-hydrolyzable ATP analog
3. as monomer, dimer, and higher-order assemblies
Reposted
New cryo-EM structures of HSV-1 origin-binding protein reveal how herpesvirus initiates DNA replication, offering new antiviral targets beyond DNA polymerase. Study published in
@narjournal.bsky.social 🧪
academic.oup.com/nar/article/...
@narjournal.bsky.social 🧪
academic.oup.com/nar/article/...
The herpes simplex origin-binding protein: mechanisms for sequence-specific DNA binding and dimerization revealed by Cryo-EM
Abstract. Herpes simplex viruses 1 and 2 (HSV-1,2) present growing treatment challenges due to increasing resistance to antivirals targeting viral DNA poly
academic.oup.com
October 24, 2025 at 1:55 PM
New cryo-EM structures of HSV-1 origin-binding protein reveal how herpesvirus initiates DNA replication, offering new antiviral targets beyond DNA polymerase. Study published in
@narjournal.bsky.social 🧪
academic.oup.com/nar/article/...
@narjournal.bsky.social 🧪
academic.oup.com/nar/article/...
Reposted
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
October 24, 2025 at 5:44 PM
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
Reposted
📜 The Herpes simplex Origin Binding Protein: Mechanisms for sequence specific DNA binding and dimerization revealed by Cryo-EM
🧑🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social
📔 Nucleic Acids Research @narjournal.bsky.social
🔗 doi.org/10.1093/nar/...
🧑🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social
📔 Nucleic Acids Research @narjournal.bsky.social
🔗 doi.org/10.1093/nar/...
The herpes simplex origin-binding protein: mechanisms for sequence-specific DNA binding and dimerization revealed by Cryo-EM
Abstract. Herpes simplex viruses 1 and 2 (HSV-1,2) present growing treatment challenges due to increasing resistance to antivirals targeting viral DNA poly
doi.org
October 23, 2025 at 6:38 AM
📜 The Herpes simplex Origin Binding Protein: Mechanisms for sequence specific DNA binding and dimerization revealed by Cryo-EM
🧑🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social
📔 Nucleic Acids Research @narjournal.bsky.social
🔗 doi.org/10.1093/nar/...
🧑🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social
📔 Nucleic Acids Research @narjournal.bsky.social
🔗 doi.org/10.1093/nar/...
Reposted
Very excited that my final postdoctoral work is now online in Cell @cellpress.bsky.social.
www.sciencedirect.com/science/arti...
Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.
www.sciencedirect.com/science/arti...
Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.
October 13, 2025 at 9:04 AM
Very excited that my final postdoctoral work is now online in Cell @cellpress.bsky.social.
www.sciencedirect.com/science/arti...
Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.
www.sciencedirect.com/science/arti...
Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.
Reposted
Locating the missing chlorophylls f in far-red photosystem I pubmed.ncbi.nlm.nih.gov/41066538/ #cryoem
October 11, 2025 at 12:25 AM
Locating the missing chlorophylls f in far-red photosystem I pubmed.ncbi.nlm.nih.gov/41066538/ #cryoem
Reposted
How do SMC complexes load onto DNA to get ready for loop extrusion?
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
October 9, 2025 at 2:43 PM
How do SMC complexes load onto DNA to get ready for loop extrusion?
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
Reposted
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
September 26, 2025 at 2:28 AM
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
Reposted
These results are truly impressive! But I do worry that #cryoEM is moving into a phase where progress is made by optimizing parameter settings for black box programs. The devil is often in the details, and I am not sure how this could be implemented in #relion, as stated, without more info.
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 21, 2025 at 7:42 AM
Reposted
Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification https://www.biorxiv.org/content/10.1101/2025.09.14.676144v1
September 17, 2025 at 9:51 PM
Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification https://www.biorxiv.org/content/10.1101/2025.09.14.676144v1
Reposted
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
September 19, 2025 at 1:36 PM
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
May 30, 2025 at 7:58 PM