Lorraine Montel
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lorrainemontel.bsky.social
Lorraine Montel
@lorrainemontel.bsky.social
38 followers 57 following 1 posts
CNRS Researcher at Institute of Light and Matter (Univ Lyon1). Insterested in mechanics and mechanotransduction in macrophages, particularly during phagocytosis.
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lorrainemontel.bsky.social
Internship opportunity for a Master Student
@symux.bsky.social and I are looking for someone to work with us to answer this tantalizing question: does a phagocyte regulates its cell volume during and after phagocytosis?

If you are interested, please do not hesitate to contact us in DM or by e-mail.
Volume control during phagocytosis Supervised by Sylvain Monnier and Lorraine Montel at Institute of Light and Matter, Lyon Scientific context: Phagocytosis is the internalization of large particles (>1μm) by immune cells to protect the organism from pathogens, foreign materials and dying cells. The cell changes shape rapidly to wrap itself around the target particle until the target is sealed in a membrane-bound compartment called the phagosome. As the internalization ends, the cell has to accommodate the volume of one or several phagosomes into its own cytoplasm, displacing organelles and squeezing the nucleus. Membrane availability and tension have been shown to be essential for this process, and phagocytosis can be stalled by the application of osmotic shocks that inflates the cell and thus decrease membrane availability. Yet the influence of the cytoplasmic volume and its regulation during and after phagocytosis is unknown. Volume is a natural feature of cells, controlled by passive and active mechanisms taking place at the plasma membrane. Cell volume is directly affecting cell density, molecular crowding, molecular concentrations and thus biochemical equilibria. Hence volume changes influence cell mechanics, progression through the cell cycle and cell decisions. In this project, we aim to measure the volume of phagocytes before, during and after phagocytosis of large particles to understand whether the cell actively regulates its own volume to accommodate larger objects. The objects will be emulsion droplets that can be modulated in size and functionalized for phagocytosis. These particles can then be presented to macrophages for phagocytosis. Measurements of the cell volume during phagocytosis will be done using FXm, using two dyes. One dye will be excluded from both the cells and the droplets and thus measure the increasing volume of a cell ingesting a particle. The other dye will match the color of the dye in the particles, and thus reveal the changes in volume of the cell itself, not accounting for the particle volume. The project will involve: • cell culture of immortalized macrophages • fabrication and functionalization of oil droplets as artificial particles for phagocytosis • microfabrication of FXm chambers • live microscopy of phagocytosis in FXm • image and data analysis The project will take place in the Biophysics team of the Institut Lumière Matière (iLM) in Lyon, under the supervision of Lorraine Montel, an expert in phagocytosis and the physico-chemistry of droplets, and Sylvain Monnier, an expert in cell volume regulation and its measurement. The institute has all the facilities required for the project (clean room, environmental microscopy, droplet production, etc.). Contacts: lorraine.montel@univ-lyon1.fr or sylvain.monnier@univ-lyon1.fr
December 2, 2025 at 4:17 PM
Reposted by Lorraine Montel
clairedessalles.bsky.social
📢Internships on cellular nematics!

🔬Come for the pretty images, stay for the cool physics: each project combines in vitro experiments with advanced microscopy and image analysis.

📧Feel free to DM or email me with any questions.
December 2, 2025 at 3:15 PM
Reposted by Lorraine Montel
juanmagararc.bsky.social
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
November 27, 2025 at 2:14 PM
Reposted by Lorraine Montel
jfattaccioli.bsky.social
We introduce a microfluidic device that can trap, analyze, and release individual microparticles on demand using membrane deformation driven by pneumatic pressure. The setup uses a deformable membrane suspended over an array of traps.

www.sciencedirect.com/science/arti...
Fully integrated automatic microfluidic setup for immobilization, analysis and non-selective release of particles
We present a microfluidic device that enables trapping, analysis and on-demand release of individual microparticles using membrane deformation induced…
www.sciencedirect.com
November 20, 2025 at 5:02 PM
Reposted by Lorraine Montel
jfattaccioli.bsky.social
We introduce a modular biosensing strategy using targeted lipid microparticles to monitor phagosomal acidification in real time. These biomimetic particles are engineered to couple receptor-specific uptake with fluorescence-based pH reporting in macrophages.

www.biorxiv.org/content/10.1...
November 20, 2025 at 5:17 PM
Reposted by Lorraine Montel
mmorrissey.bsky.social
We have TWO papers out in @jcb.org this month! 🎉

First, @kirstin-rollins.bsky.social showed that macrophages nibble adherent target cells, like the ones found in solid tumors, instead of phagocytosing. Amazing movies from @sareenfiaz.bsky.social

doi.org/10.1083/jcb....
October 17, 2025 at 5:07 PM
Reposted by Lorraine Montel
jcb.org
Target cell adhesion limits macrophage #phagocytosis and promotes #trogocytosis, say @kirstin-rollins.bsky.social, @mmorrissey.bsky.social et al.
@ucsantabarbara.bsky.social rupress.org/jcb/article/...

#Macrophages #Immunology
October 10, 2025 at 3:15 PM
Reposted by Lorraine Montel
a-berut.bsky.social
Notre article en collaboration avec des chimistes de l'IRCELyon vient d'être publié dans ACS Applied Materials and Interfaces, je peux donc maintenant officiellement écrire sur mon CV que je suis un "material scientist" 🤭
pubs.acs.org/doi/10.1021/...
Le "graphical abstract" de l'article "Copper(I)–Thiolate Coordination Polymers for In Situ Temperature Sensing in PDMS". Comme tout bon graphical abstract, il est incompréhensible si on ne sait pas déjà de quoi il s'agit. On y voit notamment : - des grandes molécules chimiques (des polyères de coordination) qui sont réduits en poudre rouge et verte respectivement - une plaque transparente éclairée par une lumière UV, sur laquelle on applique un fer à souder - deux caméras qui filment la plaque transparente et affichent le champs de température correspondant sur un écran
July 22, 2025 at 4:06 PM
Reposted by Lorraine Montel
pjsaez.bsky.social
Join us for this @dgz.bsky.social seminar online with very cool talks about the Cell biology of the immune system

There will be of course some #CellMigration of leukocytes ;)

Speakers: @liz-kurtz.bsky.social, #ZahraaA, @labliston.bsky.social and #StefanU
dgz.bsky.social DGZ.bsky.social @dgz.bsky.social · Apr 11
The next #DGZ Focus Workshop on #CellBiology of the #ImmuneSystem will take place on April 29, organized by @pjsaez.bsky.social & Evi Kiermaier – join us! 🧑‍🔬🧑‍🔬🧬
April 11, 2025 at 2:49 PM
Reposted by Lorraine Montel
florenceniedergang.bsky.social
Our latest publication on #macrophages congrats Anna Mularski and @manondepierre.bsky.social @institutcochin.bsky.social @actin-paris-saclay.bsky.social Martial Balland @grenoblealpes.bsky.social
@cnrsbiologie.bsky.social cnrs physique @anr
March 28, 2025 at 6:44 PM
Reposted by Lorraine Montel
phpuech.bsky.social
Hi to all, we have open positions in the frame of a funded ANR, nickname Criticality, one in Marseille with me, two in Paris (P. Pierobon, Cochin / J. Husson, LadHyX). Can you spread around the word to young persons who could be interested in joining our project ?
To contact me : phpuech.github.io
Immunobiophysics
What do we do ?
phpuech.github.io
March 27, 2025 at 8:36 AM
Reposted by Lorraine Montel
immunobiophysics.bsky.social
We are excited to announce our next speaker for the immunobiophysics seminar series: Renaud Poincloux, from IPBS Toulouse, France.

The seminar will take place on March 27th at 16:30 CET (3:30 PM UTC). The Zoom link will be sent to you the day before the event.
Renaud Poincloux - IPBS-Toulouse
Cellular biologist studying the actin cytoskeleton using advanced cell imaging methods. Podosomaholic. Current positionCNRS research[…]
www.ipbs.fr
March 26, 2025 at 8:56 PM
Reposted by Lorraine Montel
bladoux.bsky.social
Happy to share our new publication in
@naturematerials.bsky.social
on mechanical cell competition! Great collaboration between our CAM team, Chavrier's and Doostmohammadi's. Special congrats
@luanger.bsky.social, @andreasschoenit.bsky.social, Siavash Monfared! 🎉
www.nature.com/articles/s41...
March 14, 2025 at 12:10 PM