Lin Mei
@lmei93.bsky.social
structural biologist, protein engineer, biologics expert; Ph.D. in biochemistry and biophysics from Rockefeller University; B.S. from Peking University
Reposted by Lin Mei
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
September 26, 2025 at 2:28 AM
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
Reposted by Lin Mei
Some happy news. Pleased to see our paper showing how afadin strengthens cytoskeletal engagement by the cadherin-catenin cell adhesion complex out in Science Advances: bit.ly/3EFpD6h
Afadin mediates cadherin-catenin complex clustering on F-actin linked to cooperative binding and filament curvature
Afadin strengthens adherens junctions by stabilizing cadherin-catenin complex clusters on curved F-actin.
bit.ly
February 18, 2025 at 3:37 AM
Some happy news. Pleased to see our paper showing how afadin strengthens cytoskeletal engagement by the cadherin-catenin cell adhesion complex out in Science Advances: bit.ly/3EFpD6h
Reposted by Lin Mei
Jazzed to join the cool kids club and have our paper reporting the reconstitution of zyxin-mediated repair of contractile actin networks appear in @currentbiology.bsky.social ! bit.ly/41enkjo
Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair
Phua et al. report that LIM proteins form force-dependent assemblies that bridge broken
filament fragments. Zyxin assemblies coordinate F-actin nucleation by VASP and its
crosslinking into aligned bun...
bit.ly
February 13, 2025 at 4:15 PM
Jazzed to join the cool kids club and have our paper reporting the reconstitution of zyxin-mediated repair of contractile actin networks appear in @currentbiology.bsky.social ! bit.ly/41enkjo
Reposted by Lin Mei
My first post on this forum! 😄
I am happy to share that our #openaccess review on the #molecular mechanisms of #actin filament polymerization and dissasembly is now online @cp-trendscellbio.bsky.social
www.cell.com/trends/cell-...
Big thanks to my amazing colleagues in Dortmund
I am happy to share that our #openaccess review on the #molecular mechanisms of #actin filament polymerization and dissasembly is now online @cp-trendscellbio.bsky.social
www.cell.com/trends/cell-...
Big thanks to my amazing colleagues in Dortmund
January 23, 2025 at 9:08 AM
My first post on this forum! 😄
I am happy to share that our #openaccess review on the #molecular mechanisms of #actin filament polymerization and dissasembly is now online @cp-trendscellbio.bsky.social
www.cell.com/trends/cell-...
Big thanks to my amazing colleagues in Dortmund
I am happy to share that our #openaccess review on the #molecular mechanisms of #actin filament polymerization and dissasembly is now online @cp-trendscellbio.bsky.social
www.cell.com/trends/cell-...
Big thanks to my amazing colleagues in Dortmund
Reposted by Lin Mei
Yerba mate makes the third-most popular caffeinated drink in the world, but it’s unrelated to tea and coffee. The first reconstruction of its genome is helping us understand how multiple species have evolved to synthesise caffeine.
https://buff.ly/4jnWk82
https://buff.ly/4jnWk82
January 23, 2025 at 9:01 PM
Yerba mate makes the third-most popular caffeinated drink in the world, but it’s unrelated to tea and coffee. The first reconstruction of its genome is helping us understand how multiple species have evolved to synthesise caffeine.
https://buff.ly/4jnWk82
https://buff.ly/4jnWk82
Reposted by Lin Mei
Not entirely sure, but this is the first cryoEM structure of a full-length single-pass transmembrane protein I can think of with both intracellular and extracellular domains resolved. Either way it's nice work!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Architecture and activation of single-pass transmembrane receptor guanylyl cyclase - Nature Structural & Molecular Biology
Here the authors show the structures of single-pass transmembrane receptor guanylyl cyclase in both apo and hormone-bound states, advancing our understanding of cross-membrane signaling by single-pass...
www.nature.com
November 18, 2024 at 2:27 AM
Not entirely sure, but this is the first cryoEM structure of a full-length single-pass transmembrane protein I can think of with both intracellular and extracellular domains resolved. Either way it's nice work!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Lin Mei
To read more about Boltz-1 check out:
- Our short blog-post jclinic.mit.edu/boltz-1/
- Our technical report: gcorso.github.io/assets/boltz...
- Our code, model and instructions: github.com/jwohlwend/bo...
I'll share a separate thread with more details about the process and interesting findings!
- Our short blog-post jclinic.mit.edu/boltz-1/
- Our technical report: gcorso.github.io/assets/boltz...
- Our code, model and instructions: github.com/jwohlwend/bo...
I'll share a separate thread with more details about the process and interesting findings!
Introducing Boltz-1: Democratizing Biomolecular Interaction Modeling – MIT Jameel Clinic
jclinic.mit.edu
November 17, 2024 at 4:21 PM
To read more about Boltz-1 check out:
- Our short blog-post jclinic.mit.edu/boltz-1/
- Our technical report: gcorso.github.io/assets/boltz...
- Our code, model and instructions: github.com/jwohlwend/bo...
I'll share a separate thread with more details about the process and interesting findings!
- Our short blog-post jclinic.mit.edu/boltz-1/
- Our technical report: gcorso.github.io/assets/boltz...
- Our code, model and instructions: github.com/jwohlwend/bo...
I'll share a separate thread with more details about the process and interesting findings!