Lazare Saladin
@lazaresaladin.bsky.social
Post Doctoral Researcher at EPFL (LCBM). Synthesis of fluorescent probes for cell imaging.
Pinned
Lazare Saladin
@lazaresaladin.bsky.social
· Nov 12
Targeted Photoactivatable Green‐Emitting BODIPY Based on Directed Photooxidation‐Induced Activation and its Application to Live Dynamic Super‐Resolution Microscopy
PFB is an efficient, green-emitting, photoactivatable probe based on BODIPY. Upon irradiation with visible light (488 nm), directed photooxidation oxidizes the furanyl group and eliminates the quench....
chemistry-europe.onlinelibrary.wiley.com
Happy to see our latest work in ChemEurJ🎉. Here we present a new way to get a photoactivatable BODIPY for cell imaging. This probe allows super-resolutive images in the green channel. Special thanks to Valentine, Maxence, Pascal and Mayeul.
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Lazare Saladin
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
Reposted by Lazare Saladin
This work is now out @pnas.org. Result of fantastic collaborations with @so-lets-kilab70.bsky.social and Jason vevea @St Jude. Look out for two cool fluorescent ligands.
November 16, 2025 at 5:11 AM
This work is now out @pnas.org. Result of fantastic collaborations with @so-lets-kilab70.bsky.social and Jason vevea @St Jude. Look out for two cool fluorescent ligands.
Reposted by Lazare Saladin
Excited to share the second chapter of my PhD work - Photoclickable HaloTag Ligands for Spatiotemporal Multiplexed Protein Labeling on Living Cells! 🥳 A big thank you to @clairedeo.bsky.social and all co-authors who contributed to this work.
➡️ www.biorxiv.org/content/10.1...
➡️ www.biorxiv.org/content/10.1...
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 5:53 PM
Excited to share the second chapter of my PhD work - Photoclickable HaloTag Ligands for Spatiotemporal Multiplexed Protein Labeling on Living Cells! 🥳 A big thank you to @clairedeo.bsky.social and all co-authors who contributed to this work.
➡️ www.biorxiv.org/content/10.1...
➡️ www.biorxiv.org/content/10.1...
Reposted by Lazare Saladin
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Lazare Saladin
A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...
Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature
Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...
www.nature.com
November 6, 2025 at 2:24 PM
A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...
Reposted by Lazare Saladin
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
November 1, 2025 at 5:56 AM
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Reposted by Lazare Saladin
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Lazare Saladin
Reposted by Lazare Saladin
Happy to share our new preprint, a fantastic collaboration with @prevedel-lab.bsky.social ! We developped a probe that can switch between fluorescence and photoacoustic contrast upon illumination. Congrats to @kaydanych.bsky.social and Magda who led this project.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Bridging Light and Sound: a Spironaphtopyran-Rhodamine Dyad with High-Contrast Photoswitching Between Fluorescence and Photoacoustic Signal
Fluorescence and photoacoustic imaging are complementary modalities that provide distinct advantages for biological imaging: fluorescence microscopy offers high sensitivity and resolution, while photo...
www.biorxiv.org
October 25, 2025 at 4:09 PM
Happy to share our new preprint, a fantastic collaboration with @prevedel-lab.bsky.social ! We developped a probe that can switch between fluorescence and photoacoustic contrast upon illumination. Congrats to @kaydanych.bsky.social and Magda who led this project.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Lazare Saladin
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....
onlinelibrary.wiley.com
October 25, 2025 at 8:56 AM
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
Reposted by Lazare Saladin
Reposted by Lazare Saladin
It’s finally happening!🎉 The Reisenbauer Lab will open in February at ISTA (www.reisenbauerlab.com
).
We’re starting to build our team and are looking for enthusiastic scientists to join us.
I feel deeply grateful to my mentors, colleagues, and family for all their support. Excited for what’s next!
).
We’re starting to build our team and are looking for enthusiastic scientists to join us.
I feel deeply grateful to my mentors, colleagues, and family for all their support. Excited for what’s next!
Home | Reisenbauer Lab
The Reisenbauer Lab is a research group in biocatalysis and enzyme engineering at the IST Austria.
www.reisenbauerlab.com
October 24, 2025 at 11:43 AM
It’s finally happening!🎉 The Reisenbauer Lab will open in February at ISTA (www.reisenbauerlab.com
).
We’re starting to build our team and are looking for enthusiastic scientists to join us.
I feel deeply grateful to my mentors, colleagues, and family for all their support. Excited for what’s next!
).
We’re starting to build our team and are looking for enthusiastic scientists to join us.
I feel deeply grateful to my mentors, colleagues, and family for all their support. Excited for what’s next!
Reposted by Lazare Saladin
What can HaloTag do for you?
Triggered fluorogenic labelling?
Molecular recording?
Photo-Chemically Induced Dimerisation?
Philipp Mauker's CHalo motif lets reagents do all three, and more.
Read the preprint here -
doi.org/10.1101/2025...
#chembio #fluorescence #cellbio #SLP
Triggered fluorogenic labelling?
Molecular recording?
Photo-Chemically Induced Dimerisation?
Philipp Mauker's CHalo motif lets reagents do all three, and more.
Read the preprint here -
doi.org/10.1101/2025...
#chembio #fluorescence #cellbio #SLP
October 1, 2025 at 2:11 PM
What can HaloTag do for you?
Triggered fluorogenic labelling?
Molecular recording?
Photo-Chemically Induced Dimerisation?
Philipp Mauker's CHalo motif lets reagents do all three, and more.
Read the preprint here -
doi.org/10.1101/2025...
#chembio #fluorescence #cellbio #SLP
Triggered fluorogenic labelling?
Molecular recording?
Photo-Chemically Induced Dimerisation?
Philipp Mauker's CHalo motif lets reagents do all three, and more.
Read the preprint here -
doi.org/10.1101/2025...
#chembio #fluorescence #cellbio #SLP
Reposted by Lazare Saladin
It's official, the Young French ChemBio group has just been launched 💫 !
Our mission ? To bring together the next generation of chemical biologists 🧪🧬, foster exchange opportunities, and promote interdisciplinary science in France and beyond !
@scf-chembio.bsky.social
Our mission ? To bring together the next generation of chemical biologists 🧪🧬, foster exchange opportunities, and promote interdisciplinary science in France and beyond !
@scf-chembio.bsky.social
October 1, 2025 at 10:26 AM
It's official, the Young French ChemBio group has just been launched 💫 !
Our mission ? To bring together the next generation of chemical biologists 🧪🧬, foster exchange opportunities, and promote interdisciplinary science in France and beyond !
@scf-chembio.bsky.social
Our mission ? To bring together the next generation of chemical biologists 🧪🧬, foster exchange opportunities, and promote interdisciplinary science in France and beyond !
@scf-chembio.bsky.social
Reposted by Lazare Saladin
We are hiring!
If you are looking for a postdoc position and you are interested in signaling lipids and proteomics, come join us in beautiful Switzerland!
More information on www.epfl.ch/labs/gr-schu...
If you are looking for a postdoc position and you are interested in signaling lipids and proteomics, come join us in beautiful Switzerland!
More information on www.epfl.ch/labs/gr-schu...
Open positions
GR-SCHUHMACHER
www.epfl.ch
August 12, 2025 at 9:32 AM
We are hiring!
If you are looking for a postdoc position and you are interested in signaling lipids and proteomics, come join us in beautiful Switzerland!
More information on www.epfl.ch/labs/gr-schu...
If you are looking for a postdoc position and you are interested in signaling lipids and proteomics, come join us in beautiful Switzerland!
More information on www.epfl.ch/labs/gr-schu...
Reposted by Lazare Saladin
In our new paper in JACS Au, with the Sagara group from the Institute of Science Tokyo, we introduce flippers than respond to stretching rather than compression and applicability to the materials rather than the life sciences (GOA).
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
August 6, 2025 at 6:10 AM
In our new paper in JACS Au, with the Sagara group from the Institute of Science Tokyo, we introduce flippers than respond to stretching rather than compression and applicability to the materials rather than the life sciences (GOA).
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by Lazare Saladin
Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.
August 5, 2025 at 2:09 PM
Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.
Reposted by Lazare Saladin
#PhDposition #OrganicChemistry #ChemBio - deadline 03.08 - please RT ❤️ Application online stellenangebote.uni-marburg.de/jobposting/d...
July 29, 2025 at 12:29 PM
#PhDposition #OrganicChemistry #ChemBio - deadline 03.08 - please RT ❤️ Application online stellenangebote.uni-marburg.de/jobposting/d...
Reposted by Lazare Saladin
New paper online:
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology
By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...
www.nature.com
July 28, 2025 at 9:44 AM
New paper online:
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Reposted by Lazare Saladin
Check it out at PNAS today. Thank you Alexandre for the collaboration!!
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
July 16, 2025 at 7:42 AM
Check it out at PNAS today. Thank you Alexandre for the collaboration!!
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
Reposted by Lazare Saladin
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...
www.biorxiv.org
July 9, 2025 at 7:58 PM
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
Reposted by Lazare Saladin
The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...
June 27, 2025 at 3:41 PM
The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...
Reposted by Lazare Saladin
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted by Lazare Saladin
July 3, 2025 at 8:05 AM
Reposted by Lazare Saladin
🔬👨💻📰 #SReD is out!
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
July 2, 2025 at 7:09 AM
🔬👨💻📰 #SReD is out!
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...