Haley Lab
@labohaley.bsky.social
We develop and apply advanced genetic manipulation technologies to make sense of biological complexity. Formerly @Genentech now @umontreal.ca. More about our lab and research here: labohaley.com
Pinned
Haley Lab
@labohaley.bsky.social
· Jul 24
A novel, high-density CRISPR activation platform for mapping cancer dependencies and resistance pathways ex vivo and in vivo
CRISPR activation (CRISPRa) enables precise, locus-specific upregulation of gene expression, offering potential for both ex vivo and in vivo applications. However, the lack of scalable, high-coverage ...
www.biorxiv.org
New preprint that we're super excited to share! With @ceo.onjcri.org.au and colleagues @wehi-research.bsky.social and @ONJCRI, along with teams @Genentech, we developed + demonstrate the use of Partita, a new suite of CRISPRa libraries for murine model systems.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Haley Lab
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - @jshendure.bsky.social @troymcdiarmid.bsky.social @uwgenome.bsky.social go.nature.com/49eTPCu
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - Nature Biotechnology
Prime editing in mammalian cells benefits from a comprehensive list of genetic parts.
go.nature.com
November 11, 2025 at 4:36 PM
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording - @jshendure.bsky.social @troymcdiarmid.bsky.social @uwgenome.bsky.social go.nature.com/49eTPCu
Reposted by Haley Lab
🌍Open call: Junior Group Leader positions!
Join a world-class biomedical research institute at the heart of the Vienna BioCenter, where curiosity drives discovery.
Lead your own lab, pursue bold ideas, and shape the future of science at the IMP: www.imp.ac.at/career/open-...
Join a world-class biomedical research institute at the heart of the Vienna BioCenter, where curiosity drives discovery.
Lead your own lab, pursue bold ideas, and shape the future of science at the IMP: www.imp.ac.at/career/open-...
November 10, 2025 at 1:26 PM
🌍Open call: Junior Group Leader positions!
Join a world-class biomedical research institute at the heart of the Vienna BioCenter, where curiosity drives discovery.
Lead your own lab, pursue bold ideas, and shape the future of science at the IMP: www.imp.ac.at/career/open-...
Join a world-class biomedical research institute at the heart of the Vienna BioCenter, where curiosity drives discovery.
Lead your own lab, pursue bold ideas, and shape the future of science at the IMP: www.imp.ac.at/career/open-...
Reposted by Haley Lab
I take this example from @ceronifranci.bsky.social to highlight that we, at CSH Protocols, are also always interested in review articles like this, centered around techniques.
So, if you have an idea, let's talk!
www.sciencedirect.com/science/arti...
So, if you have an idea, let's talk!
www.sciencedirect.com/science/arti...
Transgene integration in mammalian cells: The tools, the challenges, and the future
Robust and stable expression of genes of interest is crucial for studying and engineering biology. While expressing desired gene products in some micr…
www.sciencedirect.com
October 27, 2025 at 4:32 PM
I take this example from @ceronifranci.bsky.social to highlight that we, at CSH Protocols, are also always interested in review articles like this, centered around techniques.
So, if you have an idea, let's talk!
www.sciencedirect.com/science/arti...
So, if you have an idea, let's talk!
www.sciencedirect.com/science/arti...
Reposted by Haley Lab
Transcriptional interferences ensure one olfactory receptor per ant neuron
www.nature.com/articles/s41...
Caviar for RNA-Seq nerds! Check this out
www.nature.com/articles/s41...
Caviar for RNA-Seq nerds! Check this out
October 22, 2025 at 8:40 PM
Transcriptional interferences ensure one olfactory receptor per ant neuron
www.nature.com/articles/s41...
Caviar for RNA-Seq nerds! Check this out
www.nature.com/articles/s41...
Caviar for RNA-Seq nerds! Check this out
Reposted by Haley Lab
I am proud to be a member of the #ISSCR2026 Program Committee and to have helped develop the outstanding program, taking place in Montréal, Canada on 8-11 July 2026. Register to hear the excellent science presented! invt.io/1bxbi6gtbsl @isscr.org
I am a member of the ISSCR 2026 Program Committee
Register now
invt.io
October 19, 2025 at 8:13 AM
I am proud to be a member of the #ISSCR2026 Program Committee and to have helped develop the outstanding program, taking place in Montréal, Canada on 8-11 July 2026. Register to hear the excellent science presented! invt.io/1bxbi6gtbsl @isscr.org
Reposted by Haley Lab
I can't take any credit but I am still really excited about this LNP Review in @natbiomedeng.nature.com! Targeting and tracking mRNA lipid nanoparticles at the particle, transcript and protein level | Nature Biomedical Engineering share.google/vIhr3EOMUfke...
October 17, 2025 at 9:41 PM
I can't take any credit but I am still really excited about this LNP Review in @natbiomedeng.nature.com! Targeting and tracking mRNA lipid nanoparticles at the particle, transcript and protein level | Nature Biomedical Engineering share.google/vIhr3EOMUfke...
Reposted by Haley Lab
I’m very surprised that the frequently discussed topic of repeated adaptation through the same molecular pathways hadn’t been summarised in a review before. But that has now changed, and that’s a good thing. Thank you @siribirkeland.bsky.social
www.cell.com/trends/genet...
www.cell.com/trends/genet...
Pathway-level convergence: an underexplored aspect of convergent evolution
The extent to which evolution is repeatable at the genetic level remains a central
question in biology. Convergent adaptations can arise through mutations in homologous
genes, changes in different gen...
www.cell.com
October 16, 2025 at 8:58 PM
I’m very surprised that the frequently discussed topic of repeated adaptation through the same molecular pathways hadn’t been summarised in a review before. But that has now changed, and that’s a good thing. Thank you @siribirkeland.bsky.social
www.cell.com/trends/genet...
www.cell.com/trends/genet...
Reposted by Haley Lab
We are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
October 17, 2025 at 2:56 PM
We are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
Reposted by Haley Lab
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...
September 30, 2025 at 2:43 PM
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...
Reposted by Haley Lab
The @crick.ac.uk is recruiting Early Career Group Leaders
- Lab set-up, research costs, salaries for up to 5 researchers
- Support for up to 12 years
- Access to our core facilities
- Competitive salary
- Fantastic colleagues
- All areas of biology
Deadline 27 Nov
www.crick.ac.uk/careers-stud...
- Lab set-up, research costs, salaries for up to 5 researchers
- Support for up to 12 years
- Access to our core facilities
- Competitive salary
- Fantastic colleagues
- All areas of biology
Deadline 27 Nov
www.crick.ac.uk/careers-stud...
Early career group leaders
We appoint researchers from across biology and biomedicine to set up their first groups at the Crick.
www.crick.ac.uk
October 10, 2025 at 8:20 AM
The @crick.ac.uk is recruiting Early Career Group Leaders
- Lab set-up, research costs, salaries for up to 5 researchers
- Support for up to 12 years
- Access to our core facilities
- Competitive salary
- Fantastic colleagues
- All areas of biology
Deadline 27 Nov
www.crick.ac.uk/careers-stud...
- Lab set-up, research costs, salaries for up to 5 researchers
- Support for up to 12 years
- Access to our core facilities
- Competitive salary
- Fantastic colleagues
- All areas of biology
Deadline 27 Nov
www.crick.ac.uk/careers-stud...
Reposted by Haley Lab
**POSTDOC POSITION** My lab (www.goyallab.org) at Northwestern and CZ Biohub Chicago looking for a postdoctoc in quantitative biology. We work on a range of topics, including single-cell cancer plasticity, modeling single-cell perturbations, theory, and stem-embryo models. contact via email. Pls RT
Goyal Lab
www.goyallab.org
September 19, 2025 at 10:36 AM
**POSTDOC POSITION** My lab (www.goyallab.org) at Northwestern and CZ Biohub Chicago looking for a postdoctoc in quantitative biology. We work on a range of topics, including single-cell cancer plasticity, modeling single-cell perturbations, theory, and stem-embryo models. contact via email. Pls RT
Reposted by Haley Lab
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
September 18, 2025 at 2:34 PM
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Reposted by Haley Lab
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design
The continued development of high-dimensional CRISPR screen readouts, such as single-cell RNA sequencing and high-content imaging, necessitates compact libraries to enable functional interrogation at ...
www.biorxiv.org
September 7, 2025 at 5:02 PM
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Reposted by Haley Lab
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
August 28, 2025 at 6:34 AM
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
Reposted by Haley Lab
Final call: Applications close September 2! KI is recruiting 20 Assistant Professors in a wide range of subject areas. We're looking for early-career researchers with strong scientific merits and future potential. All positions: ki.se/en/about-ki/...
August 18, 2025 at 7:45 AM
Final call: Applications close September 2! KI is recruiting 20 Assistant Professors in a wide range of subject areas. We're looking for early-career researchers with strong scientific merits and future potential. All positions: ki.se/en/about-ki/...
Reposted by Haley Lab
Our paper is now out in final form at Nature Genetics! For those who missed the preprint, we used large-scale Perturb-seq targeting transcription factors to push primary fibroblasts into diverse transcriptional states, including those observed in cell atlas studies.
August 6, 2025 at 3:14 PM
Our paper is now out in final form at Nature Genetics! For those who missed the preprint, we used large-scale Perturb-seq targeting transcription factors to push primary fibroblasts into diverse transcriptional states, including those observed in cell atlas studies.
Reposted by Haley Lab
🧪 Registrations open for the #BarcelonaBiomed conference "When Development Meets #Cancer: Origins, Models & Therapies in #ChildhoodCancer."
Organised by Dr. Alexandra Avgustinova, Dr. Cayetano González (@irbbarcelona.org) & Dr. Jaume Mora (@sjdhospitalbarcelona.org)
➡️ https://f.mtr.cool/hpdbcpiant
Organised by Dr. Alexandra Avgustinova, Dr. Cayetano González (@irbbarcelona.org) & Dr. Jaume Mora (@sjdhospitalbarcelona.org)
➡️ https://f.mtr.cool/hpdbcpiant
August 5, 2025 at 9:02 AM
🧪 Registrations open for the #BarcelonaBiomed conference "When Development Meets #Cancer: Origins, Models & Therapies in #ChildhoodCancer."
Organised by Dr. Alexandra Avgustinova, Dr. Cayetano González (@irbbarcelona.org) & Dr. Jaume Mora (@sjdhospitalbarcelona.org)
➡️ https://f.mtr.cool/hpdbcpiant
Organised by Dr. Alexandra Avgustinova, Dr. Cayetano González (@irbbarcelona.org) & Dr. Jaume Mora (@sjdhospitalbarcelona.org)
➡️ https://f.mtr.cool/hpdbcpiant
Reposted by Haley Lab
📣Faculty Position Opening!
👩🏾🔬An exciting opportunity for an Assistant Professor position in aging research at @UMontreal
🗓️Application deadline 15 September
#FacultyJobs #CdnSci #Research #Aging
medicinoxy.com/assistant-pr...
👩🏾🔬An exciting opportunity for an Assistant Professor position in aging research at @UMontreal
🗓️Application deadline 15 September
#FacultyJobs #CdnSci #Research #Aging
medicinoxy.com/assistant-pr...
Assistant Professor in Aging (University of Montreal)
medicinoxy.com
July 31, 2025 at 11:54 AM
📣Faculty Position Opening!
👩🏾🔬An exciting opportunity for an Assistant Professor position in aging research at @UMontreal
🗓️Application deadline 15 September
#FacultyJobs #CdnSci #Research #Aging
medicinoxy.com/assistant-pr...
👩🏾🔬An exciting opportunity for an Assistant Professor position in aging research at @UMontreal
🗓️Application deadline 15 September
#FacultyJobs #CdnSci #Research #Aging
medicinoxy.com/assistant-pr...
Reposted by Haley Lab
To all post-docs: The Genome Biology dept @embl.org
has an Independent faculty position. Fantastic place to set up your lab –great package: core funding, fantastic Ph.D. students, cutting edge core facilities & great colleagues. Closing date Sept 19th
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
has an Independent faculty position. Fantastic place to set up your lab –great package: core funding, fantastic Ph.D. students, cutting edge core facilities & great colleagues. Closing date Sept 19th
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
Group Leader - Genome Biology Unit
Are you ready to lead groundbreaking research in Genome Biology? Join us at EMBL! We are seeking a motivated scientist to lead an independent research group addressing exciting and original biological...
embl.wd103.myworkdayjobs.com
July 30, 2025 at 1:41 PM
To all post-docs: The Genome Biology dept @embl.org
has an Independent faculty position. Fantastic place to set up your lab –great package: core funding, fantastic Ph.D. students, cutting edge core facilities & great colleagues. Closing date Sept 19th
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
has an Independent faculty position. Fantastic place to set up your lab –great package: core funding, fantastic Ph.D. students, cutting edge core facilities & great colleagues. Closing date Sept 19th
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
Reposted by Haley Lab
last call!
submit your abstract by tonight to be selected for one of the many oral presentations at the leading European Transposon Meeting. We are very much looking forward to your contribution and attendance!
submit your abstract by tonight to be selected for one of the many oral presentations at the leading European Transposon Meeting. We are very much looking forward to your contribution and attendance!
⏰ Abstract deadline for 'The mobile genome' is 29 July!
👉 https://s.embl.org/mge25-01-bl
Join us 4–7 Nov 2025 at EMBL Heidelberg (or online) to explore the impact of TEs across biology. 🧬🔍
⭐🧑🏼🔬 24 talks + 15 flash talks from posters – don't miss out!
#EMBOMobileGenome
👉 https://s.embl.org/mge25-01-bl
Join us 4–7 Nov 2025 at EMBL Heidelberg (or online) to explore the impact of TEs across biology. 🧬🔍
⭐🧑🏼🔬 24 talks + 15 flash talks from posters – don't miss out!
#EMBOMobileGenome
July 29, 2025 at 8:41 AM
last call!
submit your abstract by tonight to be selected for one of the many oral presentations at the leading European Transposon Meeting. We are very much looking forward to your contribution and attendance!
submit your abstract by tonight to be selected for one of the many oral presentations at the leading European Transposon Meeting. We are very much looking forward to your contribution and attendance!
New preprint that we're super excited to share! With @ceo.onjcri.org.au and colleagues @wehi-research.bsky.social and @ONJCRI, along with teams @Genentech, we developed + demonstrate the use of Partita, a new suite of CRISPRa libraries for murine model systems.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A novel, high-density CRISPR activation platform for mapping cancer dependencies and resistance pathways ex vivo and in vivo
CRISPR activation (CRISPRa) enables precise, locus-specific upregulation of gene expression, offering potential for both ex vivo and in vivo applications. However, the lack of scalable, high-coverage ...
www.biorxiv.org
July 24, 2025 at 12:47 AM
New preprint that we're super excited to share! With @ceo.onjcri.org.au and colleagues @wehi-research.bsky.social and @ONJCRI, along with teams @Genentech, we developed + demonstrate the use of Partita, a new suite of CRISPRa libraries for murine model systems.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Haley Lab
Going deep on 2A peptides:
www.cell.com/cell-reports...
(Trying to pick between GSG:T2A, GSG:P2A, and furin site:GSG:T2A)
www.cell.com/cell-reports...
(Trying to pick between GSG:T2A, GSG:P2A, and furin site:GSG:T2A)
Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences
Rao et al. identified thousands of previously unknown 2A peptides across both viruses
and eukaryotes using an HMMER analysis. The authors further identified a unique class
of 2A peptides, class B, who...
www.cell.com
July 23, 2025 at 2:19 AM
Going deep on 2A peptides:
www.cell.com/cell-reports...
(Trying to pick between GSG:T2A, GSG:P2A, and furin site:GSG:T2A)
www.cell.com/cell-reports...
(Trying to pick between GSG:T2A, GSG:P2A, and furin site:GSG:T2A)
Reposted by Haley Lab
We’re thrilled to announce IGI–Bakar Labs. This new building will allow the IGI to continue expanding our impact in health and agricultural applications of #CRISPR, and will be shared with the Bakar Labs incubator for growing biotech companies. Learn more: news.berkeley.edu/2025/07/17/c...
CRISPR researchers and startup entrepreneurs will share new building in UC Berkeley's Innovation Zone - Berkeley News
A planned addition to the campus's Innovation Zone will expand lab space for the Innovative Genomics Institute and build out the largest innovation hub — Bakar Labs — at any U.S. university.
news.berkeley.edu
July 17, 2025 at 9:05 PM
We’re thrilled to announce IGI–Bakar Labs. This new building will allow the IGI to continue expanding our impact in health and agricultural applications of #CRISPR, and will be shared with the Bakar Labs incubator for growing biotech companies. Learn more: news.berkeley.edu/2025/07/17/c...
Reposted by Haley Lab
Isoform-specific single-cell perturb-seq reveals distinct functions of alternative promoters in drug response https://www.biorxiv.org/content/10.1101/2025.07.14.664827v1
July 17, 2025 at 4:33 AM
Isoform-specific single-cell perturb-seq reveals distinct functions of alternative promoters in drug response https://www.biorxiv.org/content/10.1101/2025.07.14.664827v1
Reposted by Haley Lab
Excited to share our latest by my postdoc Ben KS: we use statistical physics & Bayesian inference to model genome-wide perturbation outcomes. Remarkably, perturbation responses are encoded in gene "chatter" even before the perturbation–a fundamental insight with broad implications
shorturl.at/2LHbw
shorturl.at/2LHbw
July 6, 2025 at 10:11 PM
Excited to share our latest by my postdoc Ben KS: we use statistical physics & Bayesian inference to model genome-wide perturbation outcomes. Remarkably, perturbation responses are encoded in gene "chatter" even before the perturbation–a fundamental insight with broad implications
shorturl.at/2LHbw
shorturl.at/2LHbw