Julien Béthune
@julienbethune.bsky.social
Professor @HAW Hamburg, interested in RNA & membrane biology, enzyme engineering & proteomics
Pinned
Julien Béthune
@julienbethune.bsky.social
· Feb 27
I am now a #HappyDepositor thanks to @addgene.bsky.social ! I really like this award because you don't get nominated for it, it just reflects how useful the reagents we created are for the community. More than 100 labs world-wide use our plasmids I hope they contribute to great discoveries!
Reposted by Julien Béthune
A carol for peace.
Together with the Embassies of Germany, the United Kingdom, and France, representatives of the Ministry of Culture, and Ukrainian artists from the National House of Music and Open Opera Ukraine, performed Shchedryk
(Carol of the Bells) in the heart of Kyiv❤️
Together with the Embassies of Germany, the United Kingdom, and France, representatives of the Ministry of Culture, and Ukrainian artists from the National House of Music and Open Opera Ukraine, performed Shchedryk
(Carol of the Bells) in the heart of Kyiv❤️
December 24, 2025 at 12:15 PM
A carol for peace.
Together with the Embassies of Germany, the United Kingdom, and France, representatives of the Ministry of Culture, and Ukrainian artists from the National House of Music and Open Opera Ukraine, performed Shchedryk
(Carol of the Bells) in the heart of Kyiv❤️
Together with the Embassies of Germany, the United Kingdom, and France, representatives of the Ministry of Culture, and Ukrainian artists from the National House of Music and Open Opera Ukraine, performed Shchedryk
(Carol of the Bells) in the heart of Kyiv❤️
Reposted by Julien Béthune
immaterielles Kulturerbe "Verspätung der Deutschen Bahn"
December 12, 2025 at 10:57 AM
immaterielles Kulturerbe "Verspätung der Deutschen Bahn"
Reposted by Julien Béthune
Confused by all the histones that are cropping up in organisms that are decidedly NOT eukaryotes? check out our review - fantastic work by team NucEvo in the #Lugerlab
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
December 9, 2025 at 3:14 PM
Confused by all the histones that are cropping up in organisms that are decidedly NOT eukaryotes? check out our review - fantastic work by team NucEvo in the #Lugerlab
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
Yesterday we celebrated the graduation of the first Erasmus Mundus cohort of our international master program EMMaH - Euro-Asian Master of Medical Technology and Healthcare Business in Lille.
Congratulations to all our new graduates and good luck for your future endeavors!
Congratulations to all our new graduates and good luck for your future endeavors!
November 22, 2025 at 9:26 AM
Yesterday we celebrated the graduation of the first Erasmus Mundus cohort of our international master program EMMaH - Euro-Asian Master of Medical Technology and Healthcare Business in Lille.
Congratulations to all our new graduates and good luck for your future endeavors!
Congratulations to all our new graduates and good luck for your future endeavors!
Reposted by Julien Béthune
"Wer wird eingeladen? Wen sehen wir als „sichtbar“ und „erfolgreich“? Und wessen Leistungen übersehen wir systematisch, weil sie mehr Zeit brauchten, um dorthin zu kommen?"Tolle Reflexion @mariehuber.bsky.social zu Karriere und Kindern in der Wissenschaft seriousjourneys.substack.com/p/alleinerzi...
Alleinerziehend in der Wissenschaft: Was Dauerstellen nicht lösen
Ein Kommentar, angeregt von "Ziel erkannt, Ziel verfehlt?" von Amrei Bahr und Kristin Eichhorn
seriousjourneys.substack.com
October 15, 2025 at 7:08 AM
"Wer wird eingeladen? Wen sehen wir als „sichtbar“ und „erfolgreich“? Und wessen Leistungen übersehen wir systematisch, weil sie mehr Zeit brauchten, um dorthin zu kommen?"Tolle Reflexion @mariehuber.bsky.social zu Karriere und Kindern in der Wissenschaft seriousjourneys.substack.com/p/alleinerzi...
Reposted by Julien Béthune
It’s clear we need alternatives or at least ways to improve the situation. So, together with a really special and talented team we worked to develop this idea into “qed” a platform where you can get CONSTRUCTIVE feedback on your own work or CRITICALLY assess other people’s papers.
October 15, 2025 at 12:22 PM
It’s clear we need alternatives or at least ways to improve the situation. So, together with a really special and talented team we worked to develop this idea into “qed” a platform where you can get CONSTRUCTIVE feedback on your own work or CRITICALLY assess other people’s papers.
Reposted by Julien Béthune
Die AfD Sachsen-Anhalt fragt gezielt nach Listen postkolonialer Seminare und Lehrstühle. Wie die Universitäten beginnen, sich gegen die Angriffe zu wappnen.
AfD hetzt gegen Hochschulen: Angriff auf die Freiheit der Wissenschaft
Die AfD Sachsen-Anhalt fragt gezielt nach Listen postkolonialer Seminare und Lehrstühle. Wie die Universitäten beginnen, sich gegen die Angriffe zu wappnen.
taz.de
October 14, 2025 at 7:50 PM
Die AfD Sachsen-Anhalt fragt gezielt nach Listen postkolonialer Seminare und Lehrstühle. Wie die Universitäten beginnen, sich gegen die Angriffe zu wappnen.
Reposted by Julien Béthune
In labs, hands-on expertise is often lost because it's not written down. We leverage multimodal AI agents to capture & share expertise by analyzing video and speech to generate protocols, detect errors, and guide researchers. #AI #TeamMassSpec
📄 Preprint: doi.org/10.1101/2025... 1/🧵
📄 Preprint: doi.org/10.1101/2025... 1/🧵
Multimodal AI agents for capturing and sharing laboratory practice
We present a multimodal AI laboratory agent that captures and shares tacit experimental practice by linking written instructions with hands-on laboratory work through the analysis of video, speech, an...
doi.org
October 8, 2025 at 7:59 PM
In labs, hands-on expertise is often lost because it's not written down. We leverage multimodal AI agents to capture & share expertise by analyzing video and speech to generate protocols, detect errors, and guide researchers. #AI #TeamMassSpec
📄 Preprint: doi.org/10.1101/2025... 1/🧵
📄 Preprint: doi.org/10.1101/2025... 1/🧵
Reposted by Julien Béthune
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
www.biorxiv.org/content/10.1...
For anybody doing proximity-dependent biotinylation have a look at this reference preprint from the @gingraslab.bsky.social . Ten enzymes benchmarked in various conditions, it appears #ultraID is the enzyme of choice for combined specificity, speed and low background🧪
For anybody doing proximity-dependent biotinylation have a look at this reference preprint from the @gingraslab.bsky.social . Ten enzymes benchmarked in various conditions, it appears #ultraID is the enzyme of choice for combined specificity, speed and low background🧪
Benchmarking of proximity-dependent biotinylation enzymes across cellular compartments and time windows
Proximity-dependent biotinylation has become a powerful approach for mapping protein interactions and subcellular organization in living cells. Although a growing number of engineered biotin ligases h...
www.biorxiv.org
September 18, 2025 at 1:03 PM
www.biorxiv.org/content/10.1...
For anybody doing proximity-dependent biotinylation have a look at this reference preprint from the @gingraslab.bsky.social . Ten enzymes benchmarked in various conditions, it appears #ultraID is the enzyme of choice for combined specificity, speed and low background🧪
For anybody doing proximity-dependent biotinylation have a look at this reference preprint from the @gingraslab.bsky.social . Ten enzymes benchmarked in various conditions, it appears #ultraID is the enzyme of choice for combined specificity, speed and low background🧪
Reposted by Julien Béthune
12 September 1924 | A Hungarian Jew, Tibor "Ted” Bolgar, was born in Sárospatak.
In June 1944, he was deported to #Auschwitz from where he was taken to other camps. He was liberated during a death march from Muhldorf.
Today he turns 101. Join us in wishing him a happy birthday.
In June 1944, he was deported to #Auschwitz from where he was taken to other camps. He was liberated during a death march from Muhldorf.
Today he turns 101. Join us in wishing him a happy birthday.
September 12, 2025 at 4:35 PM
12 September 1924 | A Hungarian Jew, Tibor "Ted” Bolgar, was born in Sárospatak.
In June 1944, he was deported to #Auschwitz from where he was taken to other camps. He was liberated during a death march from Muhldorf.
Today he turns 101. Join us in wishing him a happy birthday.
In June 1944, he was deported to #Auschwitz from where he was taken to other camps. He was liberated during a death march from Muhldorf.
Today he turns 101. Join us in wishing him a happy birthday.
Reposted by Julien Béthune
Academic authors, here's a peek into the black box of journal publishing from an journal editor if you can bear it:
September 6, 2025 at 11:09 PM
Academic authors, here's a peek into the black box of journal publishing from an journal editor if you can bear it:
Reposted by Julien Béthune
Well that's amazing.
September 3, 2025 at 4:14 PM
Well that's amazing.
First time in the fair city of Béthune since 1991, glad to be back in the town that bears my name and recommended visit if visiting north of France!
September 2, 2025 at 8:32 PM
First time in the fair city of Béthune since 1991, glad to be back in the town that bears my name and recommended visit if visiting north of France!
Reposted by Julien Béthune
This is cool. CryoEM showing COPI coat containing GOLPH3, together with functional data suggests the mechanism for cargo retention/transport.
The mechanistic basis of cargo selection during Golgi maturation
Taylor, Zubkov, Ciazynska et al.
doi.org/10.1101/2025...
The mechanistic basis of cargo selection during Golgi maturation
Taylor, Zubkov, Ciazynska et al.
doi.org/10.1101/2025...
The mechanistic basis of cargo selection during Golgi maturation
The multiple cisternae of the Golgi apparatus contain resident membrane proteins crucial for lipid and protein glycosylation. How Golgi residents remain in their designated compartments despite a cons...
doi.org
June 27, 2025 at 8:16 AM
This is cool. CryoEM showing COPI coat containing GOLPH3, together with functional data suggests the mechanism for cargo retention/transport.
The mechanistic basis of cargo selection during Golgi maturation
Taylor, Zubkov, Ciazynska et al.
doi.org/10.1101/2025...
The mechanistic basis of cargo selection during Golgi maturation
Taylor, Zubkov, Ciazynska et al.
doi.org/10.1101/2025...
Reposted by Julien Béthune
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 21, 2025 at 5:19 AM
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Julien Béthune
There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...
December 16, 2024 at 9:25 AM
There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...
🧪 this one is a nice one for anybody interested in the early secretory pathway!
Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites www.biorxiv.org/content/10.1...
Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites
Trafficking from the endoplasmic reticulum to the Golgi apparatus comprises the first steps toward the correct localization of 30% of eukaryotic proteins. Coat protein complexes COPII and COPI are inv...
www.biorxiv.org
July 31, 2025 at 6:55 PM
🧪 this one is a nice one for anybody interested in the early secretory pathway!
Reposted by Julien Béthune
💥 A gut microbe metabolite that drives #atherosclerosis?
Yes—Imidazole propionate (ImP) triggers vascular inflammation without changing cholesterol.
🧪 We show the pathway—and how to block it.
nature.com/articles/s41... #OpenAccess at
@Nature
@CNIC_CARDIO
#CVD #immunosky #cardiosky #microbiomesky
Yes—Imidazole propionate (ImP) triggers vascular inflammation without changing cholesterol.
🧪 We show the pathway—and how to block it.
nature.com/articles/s41... #OpenAccess at
@Nature
@CNIC_CARDIO
#CVD #immunosky #cardiosky #microbiomesky
Imidazole propionate is a driver and therapeutic target in atherosclerosis - Nature
Imidazole propionate produced by gut microbiota is associated with atherosclerosis in mouse models and in humans, and causes the development of atherosclerosis through activation of the imidazoline-1 ...
nature.com
July 16, 2025 at 5:37 PM
💥 A gut microbe metabolite that drives #atherosclerosis?
Yes—Imidazole propionate (ImP) triggers vascular inflammation without changing cholesterol.
🧪 We show the pathway—and how to block it.
nature.com/articles/s41... #OpenAccess at
@Nature
@CNIC_CARDIO
#CVD #immunosky #cardiosky #microbiomesky
Yes—Imidazole propionate (ImP) triggers vascular inflammation without changing cholesterol.
🧪 We show the pathway—and how to block it.
nature.com/articles/s41... #OpenAccess at
@Nature
@CNIC_CARDIO
#CVD #immunosky #cardiosky #microbiomesky
Reposted by Julien Béthune
Are bar plots without error bars or individual data really the future?
While I agree on many things said here, I don't think that the chosen examples of bar plots are a good way forward
While I agree on many things said here, I don't think that the chosen examples of bar plots are a good way forward
I gave in! After students asking for it, I now made a simple figure design checklist.
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
A checklist for designing and improving the visualization of scientific data
Nature Cell Biology - Creating clear and engaging scientific figures is crucial to communicate complex data. In this Comment, I condense principles from design, visual perception and data...
rdcu.be
June 19, 2025 at 12:06 PM
Are bar plots without error bars or individual data really the future?
While I agree on many things said here, I don't think that the chosen examples of bar plots are a good way forward
While I agree on many things said here, I don't think that the chosen examples of bar plots are a good way forward
The magician in Hamburg earlier this week
June 19, 2025 at 8:46 PM
The magician in Hamburg earlier this week
Reposted by Julien Béthune
Program now available for 3rd INTERNATIONAL TOP-DOWN PROTEOMICS SYMPOSIUM in Hamburg, Germany August 26-28 and DEADLINES approaching:
>Early Registration ends - JUNE 30
>For Oral Abstract Submissions – JUNE 30
>For Poster Abstract Submissions – JULY 15
Get the Program here >> publ.cc/UFWQwg
>Early Registration ends - JUNE 30
>For Oral Abstract Submissions – JUNE 30
>For Poster Abstract Submissions – JULY 15
Get the Program here >> publ.cc/UFWQwg
June 19, 2025 at 7:56 PM
Program now available for 3rd INTERNATIONAL TOP-DOWN PROTEOMICS SYMPOSIUM in Hamburg, Germany August 26-28 and DEADLINES approaching:
>Early Registration ends - JUNE 30
>For Oral Abstract Submissions – JUNE 30
>For Poster Abstract Submissions – JULY 15
Get the Program here >> publ.cc/UFWQwg
>Early Registration ends - JUNE 30
>For Oral Abstract Submissions – JUNE 30
>For Poster Abstract Submissions – JULY 15
Get the Program here >> publ.cc/UFWQwg
Reposted by Julien Béthune
We developed CrossFilt, a read-level, cross-species filtering approach that avoids these errors without sacrificing usable data. It outperforms all existing strategies and exposes just how much signal in comparative genomics has been misread.
doi.org/10.1101/2025...
doi.org/10.1101/2025...
CrossFilt: A Cross-species Filtering Tool that Eliminates Alignment Bias in Comparative Genomics Studies
Comparative functional genomic studies are often affected by biased read mapping across species due to inter-species differences in genome structure, sequence composition, and annotation quality. We d...
doi.org
June 7, 2025 at 12:07 AM
We developed CrossFilt, a read-level, cross-species filtering approach that avoids these errors without sacrificing usable data. It outperforms all existing strategies and exposes just how much signal in comparative genomics has been misread.
doi.org/10.1101/2025...
doi.org/10.1101/2025...