Isabelle Busseau
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isab19.bsky.social
Isabelle Busseau
@isab19.bsky.social
77 followers 220 following 3 posts
French CNRS researcher - Drosophila genetics - gene regulation mediated by small RNA
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Reposted by Isabelle Busseau
crisprflydesign.bsky.social
High levels of Cas9 are toxic in sensory neurons. Reducing Cas9 levels with uORFs avoids toxicity and is compatible with efficient editing. From @thompsonpeerlab.bsky.social. Fly lines @vdrc-flies.bsky.social

#CRISPR #Drosophila

www.biorxiv.org/content/10.1...
Figure from the preprint showing sensory neuron morphology with different levels of Cas9 expression.
October 4, 2025 at 9:10 PM
Reposted by Isabelle Busseau
flybase.bsky.social
The FlyBase situation has made the national news www.nbcnews.com/science/scie...
Trump's Harvard cuts threaten a giant in biomedical research: A database about the tiny fruit fly
This spring, the Trump administration rescinded a grant that maintained FlyBase, a pillar of global basic research, as part of its broader $2.2 billion funding cuts at Harvard.
www.nbcnews.com
September 16, 2025 at 1:08 PM
Reposted by Isabelle Busseau
drglam.bsky.social
The situation with FlyBase has made the national news The FlyBase situation has made the national news www.nbcnews.com/science/scie...
Trump's Harvard cuts threaten a giant in biomedical research: A database about the tiny fruit fly
This spring, the Trump administration rescinded a grant that maintained FlyBase, a pillar of global basic research, as part of its broader $2.2 billion funding cuts at Harvard.
www.nbcnews.com
September 16, 2025 at 1:10 PM
Reposted by Isabelle Busseau
juliusbrennecke.bsky.social
the history describing the discovery of flamenco is nicely summarized here:
www.frontiersin.org/journals/gen...
August 17, 2025 at 7:42 AM
Reposted by Isabelle Busseau
flybase.bsky.social
FlyBase needs your help! We ask that European labs continue to contribute to Cambridge, UK FlyBase, whereas US and other non-European labs can contribute to US FlyBase. For more information and how to donate: wiki.flybase.org/wiki/FlyBase...
FlyBase:Contribute to FlyBase - FlyBase Wiki
wiki.flybase.org
August 15, 2025 at 12:45 PM
Reposted by Isabelle Busseau
lucksmith.bsky.social
thank you for all your efforts on behalf of the fly community!
August 14, 2025 at 2:00 PM
Reposted by Isabelle Busseau
hansonmark.bsky.social
A reminder you/your lab can support FlyBase at Cambridge through the following link. Every bit helps. Please share if you yourself can't donate.

www.philanthropy.cam.ac.uk/give-to-camb...
August 14, 2025 at 5:25 AM
Reposted by Isabelle Busseau
biorxiv-genetic.bsky.social
The ORB2 RNA-binding protein represses translation of its target transcripts during the Drosophila maternal-to-zygotic transition via its functionally conserved Zinc binding 'ZZ' domain https://www.biorxiv.org/content/10.1101/2025.07.10.664187v1
July 14, 2025 at 3:31 AM
Reposted by Isabelle Busseau
interactivefly.bsky.social
Chen, R., Stainier, W., Dufourt, J., Lagha, M., Lehmann, R. (2024). Direct observation of translational activation by a ribonucleoprotein granule. Nat Cell Biol, 26(8):1322-1335 www.nature.com/articles/s41...
Direct observation of translational activation by a ribonucleoprotein granule - Nature Cell Biology
Chen et al. perform single-molecule imaging of translation at ribonucleoprotein (RNP) granules. They show that RNP granule surfaces are sites of nanos mRNA translation, whereas the granule interior is...
www.nature.com
June 28, 2025 at 12:27 AM
Reposted by Isabelle Busseau
flybase.bsky.social
MassLive.com recently interviewed several Harvard FlyBase people and wrote this news article about the importance of FlyBase: tinyurl.com/4chpts9f
Why a database of bug genes could be one of Trump’s most devastating cuts at Harvard
FlyBase, the world's only fruit fly database, is in the crosshairs of Trump's attack on Harvard. Here's why it could matter for your health.
tinyurl.com
June 25, 2025 at 8:31 PM
Reposted by Isabelle Busseau
crisprflydesign.bsky.social
Why use #CRISPR in #Drosophila when RNAi works so well? Because for some genes well is not good enough.

Check out new preprint from the lab demonstrating a role for Vps15 in Wingless trafficking. Something that was missed in several RNAi screens.

www.biorxiv.org/content/10.1...
Images from the linked preprint showing Wingless stainings in the Drosophila wing imaginal disc. Targeting vps15 with two indepependent RNAi lines (paper also shows the same with a third line) does not result in a phenotype. In contrast, targeting vps15 with two independent sgRNA constructs and Cas9 results in strong accumulation of Wingless protein in perturbed cells.
May 26, 2025 at 7:29 AM
Reposted by Isabelle Busseau
poppi62.bsky.social
Tired of having to explain this again and again, I made an info- graphic of #Drosophila stock keeping. Any comments? @bdsc.bsky.social @flybase.bsky.social
January 13, 2025 at 9:01 AM
Reposted by Isabelle Busseau
cellpress.bsky.social
"We should include eugenics in our undergraduate classes, reminding students that scientists must speak out when others lie about science and use it to further their political views," says professor @peiferlabunc.bsky.social.

www.cell.com/trends/genet...

@cp-trendsgenetics.bsky.social
March 31, 2025 at 5:04 PM
Reposted by Isabelle Busseau
karinajouravleva.bsky.social
I am pleased to announce the upcoming thematic school on small regulatory RNAs. It will take place in Sète from October 6–10, 2025. Pre-registration is open until June 6. For further information, please refer to the attached flyer and visit: internat25.sciencesconf.org. @cnrsbiologie.bsky.social
March 30, 2025 at 9:57 AM
Reposted by Isabelle Busseau
flybase.bsky.social
FlyBase made a fly gene themed crossword puzzle for #Dros25. If you missed getting one (we have lots at the FlyBase booth), or you couldn’t be here, it’s also available online. puzzleme.amuselabs.com/pmm/crosswor...
Play this Crossword - Each answer is the full name of a fly gene
Each answer is the full name of a fly gene
puzzleme.amuselabs.com
March 20, 2025 at 9:32 PM
Reposted by Isabelle Busseau
standupforscifr.bsky.social
#StandUpForScience a rassemblé près de 15 000 personnes partout en France. Des scientifiques, équipes, laboratoires, ou des associations citoyennes ont affiché leur soutien par des photographies prises sur leur lieu de réunion ou de travail. Nous les avons assemblées 👇
standupforscience.fr/photos/
Stand Up For Science France
standupforscience.fr
March 10, 2025 at 11:44 AM
Reposted by Isabelle Busseau
hansonmark.bsky.social
This #InternationalWomensDay I want to tell the story of Lilian Vaughan Morgan. I learned of Lilian this past year - what a badass.

Lilian was a leading scientist when women weren't welcome in the lab, nor even the department. But she contributed immensely to the genetics of sex determination

1/n
A photo of a young Lilian Vaughan Morgan, sitting with a book on her lab staring into the distance. A photo of an older Lilian Vaughan Morgan, sitting at a lab bench with a microscope and dozens of glass milk bottles bunged with cotton. Lilian is in the middle of applying ether to anaesthetize a bottle of flies.
March 8, 2025 at 10:39 AM
Reposted by Isabelle Busseau
francoisleulier.bsky.social
💪🇺🇸🇫🇷
standupforscifr.bsky.social Stand Up For Science France @standupforscifr.bsky.social · Mar 4
Press release from Stand Up For Science France (cc @standupforscience.bsky.social)
Standing Up For Science in France In the face of Trump’s administration’s relentless attacks on democratic institutions, civil rights, science, public health agencies, environmental regulation, and climate data collection, and as universities and research institutions across Europe also come under increasing pressure, the French scientific community is taking action. On March 7, 2025, scientists, educators, students, and concerned citizens will march and rally in cities across France to defend science and the humanities as pillars of democracy. This mobilization is organized in solidarity with the "Stand Up For Science" movement in the US.
March 6, 2025 at 6:57 PM
Reposted by Isabelle Busseau
karinajouravleva.bsky.social
Our review on small endogenous RNAs in animals is online: www.nature.com/articles/s41.... We thank @martinjsimard.bsky.social, @svobodalab.bsky.social and the third, anonymous, reviewer for helping us improve our manuscript. @lbmcinlyon.bsky.social @cnrsbiologie.bsky.social
A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals - Nature Reviews Molecular Cell Biology
Many new small RNA species and their derived fragments have been recently identified in animals. This Review discusses the biogenesis and functions of both structural small RNAs, such as tRNAs and sma...
www.nature.com
January 24, 2025 at 9:38 PM
Reposted by Isabelle Busseau
dschneidermann.bsky.social
Wikipedia, trop libre pour le libertarien Musk.

J'ai donné, et je vais passer au don mensuel.

Il dépend de chacun de sauver Wikipedia.
joshuaholland.bsky.social Joshua Holland @joshuaholland.bsky.social · Dec 25
I’ve never donated to Wikipedia before but I set up a small monthly donation as a fuck you to the world’s richest psychopath.
Elon Musk takes aim at Wikipedia
Musk has denounced Wikipedia as "Wokepedia" on X and urged people not to donate to the platform.
www.newsweek.com
December 26, 2024 at 2:34 PM
Reposted by Isabelle Busseau
iamshef.bsky.social
Calling #Drosophila #grad students #postdoc and #pretenure #faculty to participate in FlyCROSS and be matched by a #mentor of your preferences to gain guidance to your unique needs !
#genetics #dros25 #phdlife

@flybase.bsky.social @bdsc.bsky.social
December 6, 2024 at 4:37 PM
Reposted by Isabelle Busseau
fly-jedi.bsky.social
We are thrilled to announce that IRL JEDI meetings will restart in 2025.
You can already register at flyjedi.sciencesconf.org where you'll find a preliminary program.
Looking forward to meeting you and restart this network of fly people!
Junior European Drosophila Investigator 10th meeting - Sciencesconf.org
flyjedi.sciencesconf.org
December 20, 2024 at 6:19 PM
Reposted by Isabelle Busseau
atinygreencell.bsky.social
Come with me as I troubleshoot this very high GC PCR toward clonable homology arms in an actinobacteria. Testing DMSO, Betaine 1M, and Propylene Glycol to see if we get amplification. Arms are 1000bp. Full length with arms and gene is 2800bp. Let's go!

🧵 1/n
December 11, 2024 at 5:26 PM
Reposted by Isabelle Busseau
joelmarthelot.bsky.social
Fresh off the press, our work on wing deployment in Drosophila 🪰:
www.nature.com/articles/s41...
Work by: Simon Hadjaje, Ignacio Andrade-Silva, Marie-Julie Dalbe and Raphaël Clément
December 11, 2024 at 2:00 PM
Reposted by Isabelle Busseau
aemonten.bsky.social
"We report that numerous “miRNAs” previously reported in EMT and cancer contexts are not incorporated into RISC and are not capable of endogenously silencing target genes, despite the fact that hundreds of publications in the cancer field describe their roles"

www.biorxiv.org/content/10.1...
www.biorxiv.org
December 12, 2024 at 10:21 PM