Sebastian S. Cocioba
@atinygreencell.bsky.social
Amateur 🌿 Biologist • Aspiring 🌻 Designer
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
Pinned
Sebastian's Biotech Bazaar
Designer of fine, hand-crafted, bespoke, and fully open source biotechnology tools for educators and researchers. Plasmids, lab equipment, cell lines, and seeds. An ever-growing collection of high qua...
ATinyGreenCell.com
Excited to announce some new plasmid offerings in my shop at atinygreencell.com! Here's a thread of the current catalog. All proceeds directly support my lab and my continued effort to build useful open-source tools. Feel free to get in touch if you want to know more. Please share! 💚
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I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
November 11, 2025 at 5:33 AM
I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
Reposted by Sebastian S. Cocioba
Excited to share this perspective article out now! Come read about Streptocarpus and other great model systems!
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration
ABSTRACT. The ability of organisms to replace and regenerate anatomical structures following their loss or damage has piqued the curiosity of biologists for centuries. In addition to Development's ‘Mo...
journals.biologists.com
October 28, 2025 at 1:23 PM
Excited to share this perspective article out now! Come read about Streptocarpus and other great model systems!
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
November 10, 2025 at 6:20 PM
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
November 10, 2025 at 5:35 PM
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
November 10, 2025 at 3:52 PM
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
November 10, 2025 at 4:17 AM
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.
November 10, 2025 at 2:53 AM
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.
S-stock solution prepped 🤢
November 10, 2025 at 12:40 AM
S-stock solution prepped 🤢
Another reason petunia so good is that it can tell you the pollination worked in 24hrs or less. Flower starts to wilt if pollen took. So convenient for crossing.
November 9, 2025 at 9:14 PM
Another reason petunia so good is that it can tell you the pollination worked in 24hrs or less. Flower starts to wilt if pollen took. So convenient for crossing.
So many varieties out of this mutagenesis run!
November 9, 2025 at 8:57 PM
So many varieties out of this mutagenesis run!
YOU DO NOT NEED A GROWERS LICENSE IN NYS TO SELL SEEDS!!!
OMG I CAN GO TO MARKET TODAY
OMG I CAN GO TO MARKET TODAY
Registering my home lab so I can grow my mutant plants for sale later next year! It's so nice working with non-gm plants since you can just go to market immediately. Gonna try to make some revenue to fund the harder projects I have baking.
Yay, bootstrapped flowers!
Yay, bootstrapped flowers!
November 9, 2025 at 7:18 PM
YOU DO NOT NEED A GROWERS LICENSE IN NYS TO SELL SEEDS!!!
OMG I CAN GO TO MARKET TODAY
OMG I CAN GO TO MARKET TODAY
Registering my home lab so I can grow my mutant plants for sale later next year! It's so nice working with non-gm plants since you can just go to market immediately. Gonna try to make some revenue to fund the harder projects I have baking.
Yay, bootstrapped flowers!
Yay, bootstrapped flowers!
November 9, 2025 at 6:28 PM
Registering my home lab so I can grow my mutant plants for sale later next year! It's so nice working with non-gm plants since you can just go to market immediately. Gonna try to make some revenue to fund the harder projects I have baking.
Yay, bootstrapped flowers!
Yay, bootstrapped flowers!
Designed a quick mod to my gene gun's petri dish caddy to hold my 60mm autoclavable bead box dishes. It also supports standard 100mm, with a modest 2mm rise in elevation. Yay, rapid prototyping!
November 9, 2025 at 4:50 PM
Designed a quick mod to my gene gun's petri dish caddy to hold my 60mm autoclavable bead box dishes. It also supports standard 100mm, with a modest 2mm rise in elevation. Yay, rapid prototyping!
Nothing like a heap of unoptimized variables to keep my neurodivergent brain entertained, lol.
Trying to decide which to follow first. I'm thinking to eliminate the really basic ones first like:
Does plasmolyzing leaf disks cause necrosis in culture later?
binomicalabs.notion.site/Whisker-Tran...
Trying to decide which to follow first. I'm thinking to eliminate the really basic ones first like:
Does plasmolyzing leaf disks cause necrosis in culture later?
binomicalabs.notion.site/Whisker-Tran...
Whisker Transformation Pilot in Alice | Notion
Aim
binomicalabs.notion.site
November 9, 2025 at 3:53 PM
Nothing like a heap of unoptimized variables to keep my neurodivergent brain entertained, lol.
Trying to decide which to follow first. I'm thinking to eliminate the really basic ones first like:
Does plasmolyzing leaf disks cause necrosis in culture later?
binomicalabs.notion.site/Whisker-Tran...
Trying to decide which to follow first. I'm thinking to eliminate the really basic ones first like:
Does plasmolyzing leaf disks cause necrosis in culture later?
binomicalabs.notion.site/Whisker-Tran...
Oooh, exciting results! Seems the whiskering and/or (most likely) vortexing caused significant trauma to the leaf disks. Its not the bleach bc disks from same leaf plated immediately are perfectly green. Cooooool, got something to optimize for. Next up is to see if vortex alone.
November 9, 2025 at 3:18 PM
Oooh, exciting results! Seems the whiskering and/or (most likely) vortexing caused significant trauma to the leaf disks. Its not the bleach bc disks from same leaf plated immediately are perfectly green. Cooooool, got something to optimize for. Next up is to see if vortex alone.
Reposted by Sebastian S. Cocioba
I am trying to find everyone working on plant genomics:
go.bsky.app/NqARJ7V
Please let me know who is already on @bsky.app, but missing in this #PlantGenomics starter pack
go.bsky.app/NqARJ7V
Please let me know who is already on @bsky.app, but missing in this #PlantGenomics starter pack
February 28, 2025 at 7:39 AM
I am trying to find everyone working on plant genomics:
go.bsky.app/NqARJ7V
Please let me know who is already on @bsky.app, but missing in this #PlantGenomics starter pack
go.bsky.app/NqARJ7V
Please let me know who is already on @bsky.app, but missing in this #PlantGenomics starter pack
I'm gonna throw some tungsten at petunia leaf disks tomorrow! Will document and share. Shooting in pCAMBIA2300-RUBYRED, my standard candle for transformation.
November 9, 2025 at 5:10 AM
I'm gonna throw some tungsten at petunia leaf disks tomorrow! Will document and share. Shooting in pCAMBIA2300-RUBYRED, my standard candle for transformation.
How much do y'all pay for syringe filters? I can't really find a supplier selling new sterile PES filters for less than ~$1/filter. Thoughts?
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
November 9, 2025 at 3:40 AM
How much do y'all pay for syringe filters? I can't really find a supplier selling new sterile PES filters for less than ~$1/filter. Thoughts?
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
Would be cool to see vertical slice glimpses of what a culture we'd have across different countries if what currently makes the most money was not Ai.
What if biotech was invested in at the pace of Ai saasworks? What if material science was?
Fusion?
water catalysts?
Solar+30%?
What if biotech was invested in at the pace of Ai saasworks? What if material science was?
Fusion?
water catalysts?
Solar+30%?
November 8, 2025 at 10:04 PM
Would be cool to see vertical slice glimpses of what a culture we'd have across different countries if what currently makes the most money was not Ai.
What if biotech was invested in at the pace of Ai saasworks? What if material science was?
Fusion?
water catalysts?
Solar+30%?
What if biotech was invested in at the pace of Ai saasworks? What if material science was?
Fusion?
water catalysts?
Solar+30%?
At 2g psilocybin (B+), I grappled with my ego, stepped outside myself, felt more connected to the living world, and cried my eyes out for a few hours over mistakes, failings, and carelessness ive done in my life. It changed my world view and brought me peace.
Powerful medicine.
Powerful medicine.
November 8, 2025 at 5:02 PM
At 2g psilocybin (B+), I grappled with my ego, stepped outside myself, felt more connected to the living world, and cried my eyes out for a few hours over mistakes, failings, and carelessness ive done in my life. It changed my world view and brought me peace.
Powerful medicine.
Powerful medicine.
So much gauche disrespect for a very serious medicine. So much spectacle and downplaying. Imagine doing this for a chemotheraputic or last line antibiotic. Psilocybin can rewire neurons. It ***changes*** you. Stop this techbro hype nonsense and respect the chemistry ffs.
November 8, 2025 at 4:36 PM
So much gauche disrespect for a very serious medicine. So much spectacle and downplaying. Imagine doing this for a chemotheraputic or last line antibiotic. Psilocybin can rewire neurons. It ***changes*** you. Stop this techbro hype nonsense and respect the chemistry ffs.
I switched agar to save some cash and the snapdragons hate it, lol. Too dense. Gonna try for 6g/L instead of 7, at pH 5.70.
November 8, 2025 at 4:26 PM
I switched agar to save some cash and the snapdragons hate it, lol. Too dense. Gonna try for 6g/L instead of 7, at pH 5.70.
My mutant tricot is bloomin!!!
November 8, 2025 at 3:37 PM
My mutant tricot is bloomin!!!
Although I respect Mendel, I much prefer a tweezer over a brush. Way more efficient. Just pluck off the anther and dab it on the stigma directly.
November 8, 2025 at 3:20 PM
Although I respect Mendel, I much prefer a tweezer over a brush. Way more efficient. Just pluck off the anther and dab it on the stigma directly.