Sebastian S. Cocioba
@atinygreencell.bsky.social
Flower Designer at Neoplants 🌺
Researcher at Binomica Labs
Degreeless Heathen
Petunia and Snapdragon Breeder
Open Lab Notebook: http://tinyurl.com/ATGCFFE
Researcher at Binomica Labs
Degreeless Heathen
Petunia and Snapdragon Breeder
Open Lab Notebook: http://tinyurl.com/ATGCFFE
Pinned
Sebastian's Biotech Bazaar
Designer of fine, hand-crafted, bespoke, and fully open source biotechnology tools for educators and researchers. Plasmids, lab equipment, cell lines, and seeds. An ever-growing collection of high qua...
ATinyGreenCell.com
Excited to announce some new plasmid offerings in my shop at atinygreencell.com! Here's a thread of the current catalog. All proceeds directly support my lab and my continued effort to build useful open-source tools. Feel free to get in touch if you want to know more. Please share! 💚
1/7
1/7
I have a lot less free time now but I still want to continue my projects. Help me decide what to work on in Q1 2026!
A: Sucrose Selection System
B: 24-Well Plate Reader
C: Carbide Whisker Plant Transformation
D: Other in Comments
TYIA! 💚
A: Sucrose Selection System
B: 24-Well Plate Reader
C: Carbide Whisker Plant Transformation
D: Other in Comments
TYIA! 💚
January 6, 2026 at 2:11 AM
I have a lot less free time now but I still want to continue my projects. Help me decide what to work on in Q1 2026!
A: Sucrose Selection System
B: 24-Well Plate Reader
C: Carbide Whisker Plant Transformation
D: Other in Comments
TYIA! 💚
A: Sucrose Selection System
B: 24-Well Plate Reader
C: Carbide Whisker Plant Transformation
D: Other in Comments
TYIA! 💚
Of course, why wouldn't agar also impact AMR genes too. Everything's a variable, lol. Good preprint:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Agar concentration affects the evolution of antibiotic resistance and population genomics in experimental populations Pseudomonas aeruginosa
Bacteria live in a diversity of spatially structured environments, which can impact their evolutionary dynamics via local interactions and environmental variation. Spatial structure is expected to slo...
www.biorxiv.org
January 6, 2026 at 1:12 AM
Of course, why wouldn't agar also impact AMR genes too. Everything's a variable, lol. Good preprint:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sebastian S. Cocioba
I'm so stoked I'm getting access to a 100+ year old sourdough starter culture and you KNOW what I'm going to do with it
January 5, 2026 at 6:23 AM
I'm so stoked I'm getting access to a 100+ year old sourdough starter culture and you KNOW what I'm going to do with it
Reposted by Sebastian S. Cocioba
I’m deeply moved by the search for life in the Universe. 8yr old me would be very proud of us. The fact that we might need 1000yrs to find them - if they’re even out there - is strong motivation to start looking!
I want to try something:
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
January 5, 2026 at 2:06 AM
I’m deeply moved by the search for life in the Universe. 8yr old me would be very proud of us. The fact that we might need 1000yrs to find them - if they’re even out there - is strong motivation to start looking!
Reposted by Sebastian S. Cocioba
Yeah the science is awesome and keeps me going and all, but what makes me proud? Def seeing my students learn/tackle complex problems/have their hard work pay off.
January 5, 2026 at 3:55 AM
Yeah the science is awesome and keeps me going and all, but what makes me proud? Def seeing my students learn/tackle complex problems/have their hard work pay off.
Feeling exceptionally motivated to reboot my carbide whisker transformation attempts this week. I want to squeeze in some more tests with two new minimal payloads with visual markers asap.
January 5, 2026 at 1:59 AM
Feeling exceptionally motivated to reboot my carbide whisker transformation attempts this week. I want to squeeze in some more tests with two new minimal payloads with visual markers asap.
Reposted by Sebastian S. Cocioba
my second proudest moment was publishing work disproving a nobel laureate's claims it couldnt happen
my proudest moment was getting the cover of Science as a student tech with my second ever SEM image
both stories would doxx me so maybe one day we can have a beer together ill relive the glory ✌️
my proudest moment was getting the cover of Science as a student tech with my second ever SEM image
both stories would doxx me so maybe one day we can have a beer together ill relive the glory ✌️
January 5, 2026 at 12:49 AM
my second proudest moment was publishing work disproving a nobel laureate's claims it couldnt happen
my proudest moment was getting the cover of Science as a student tech with my second ever SEM image
both stories would doxx me so maybe one day we can have a beer together ill relive the glory ✌️
my proudest moment was getting the cover of Science as a student tech with my second ever SEM image
both stories would doxx me so maybe one day we can have a beer together ill relive the glory ✌️
I want to try something:
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
January 5, 2026 at 12:40 AM
I want to try something:
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
Post what makes you proud when working as an academic. Why do you keep at your question(s) even when things get rough and/or dataless?
Would love to hear of valiant science in spite of and despite of the current everything. Even if WIP or failed attempts; lore must be told
Makin friends
January 5, 2026 at 12:10 AM
Makin friends
January 4, 2026 at 3:39 PM
Reposted by Sebastian S. Cocioba
Testing out my Pocket qPCR with another round of experiments. This time I am trying different concentrations of GelGreen in 25uL TAQ reactions to see which gives the maximum signal without clipping the maximum saturation of the well sensors.
December 31, 2025 at 5:56 PM
Testing out my Pocket qPCR with another round of experiments. This time I am trying different concentrations of GelGreen in 25uL TAQ reactions to see which gives the maximum signal without clipping the maximum saturation of the well sensors.
Has anyone sequenced Agrobacterium rhizogenes K599? If no I'll send mine to @plasmidsaurus.bsky.social for sequencing as my first experiment of the New Year and share the results + genbank upload. Gonna prepare cells today either way.
a computer generated image of a dna structure
ALT: a computer generated image of a dna structure
media.tenor.com
December 31, 2025 at 4:52 PM
Has anyone sequenced Agrobacterium rhizogenes K599? If no I'll send mine to @plasmidsaurus.bsky.social for sequencing as my first experiment of the New Year and share the results + genbank upload. Gonna prepare cells today either way.
Walkin my mom through my traditional cloning workflow so she can see how the parts fit and how the gel bands were predicted by SnapGene ahead of the run. She enjoys learning and is surprisingly quick to understand the nuance. I work all day so this is how we bond.
December 30, 2025 at 12:07 AM
Walkin my mom through my traditional cloning workflow so she can see how the parts fit and how the gel bands were predicted by SnapGene ahead of the run. She enjoys learning and is surprisingly quick to understand the nuance. I work all day so this is how we bond.
Reposted by Sebastian S. Cocioba
Though I do paid work as an academic, that role has nothing to do with plants.
This project definitely could be published as a research paper (especially once I produce blue versions of runner beans or lima beans), but that is only likely if some lab decides to collaborate with me on it.
This project definitely could be published as a research paper (especially once I produce blue versions of runner beans or lima beans), but that is only likely if some lab decides to collaborate with me on it.
A few years ago, through luck and vibes, I managed to produce this blue colored bean variety.
This was an early step in trying to answer the question, [paraphrased] "Why isn't blue as common as red in beans, even though they're both anthocyanins and the plant has the genes for both?"
This was an early step in trying to answer the question, [paraphrased] "Why isn't blue as common as red in beans, even though they're both anthocyanins and the plant has the genes for both?"
December 29, 2025 at 7:43 AM
Though I do paid work as an academic, that role has nothing to do with plants.
This project definitely could be published as a research paper (especially once I produce blue versions of runner beans or lima beans), but that is only likely if some lab decides to collaborate with me on it.
This project definitely could be published as a research paper (especially once I produce blue versions of runner beans or lima beans), but that is only likely if some lab decides to collaborate with me on it.
Testing to see if I can use InstantOcean aquarium salts with my sucrose selection system. Would be a much easier and cheap minimal media. Just add nitrogen and carbon. I washed a colony 2x in PBS and then spiked. ASW is artificial sea water. G is glucose. S is sucrose. 🍬🦠🌊
December 29, 2025 at 5:03 AM
Testing to see if I can use InstantOcean aquarium salts with my sucrose selection system. Would be a much easier and cheap minimal media. Just add nitrogen and carbon. I washed a colony 2x in PBS and then spiked. ASW is artificial sea water. G is glucose. S is sucrose. 🍬🦠🌊
I love my lil sucrose selection system! Its jank and slow but progress is progress. High copy cscA invertase expression is enough to confer growth of E. coli K-12 on M9 minimal media where sucrose is the sole carbon source. Acidification of media adds evidence atop growth. Yay!
December 29, 2025 at 1:56 AM
I love my lil sucrose selection system! Its jank and slow but progress is progress. High copy cscA invertase expression is enough to confer growth of E. coli K-12 on M9 minimal media where sucrose is the sole carbon source. Acidification of media adds evidence atop growth. Yay!
Reposted by Sebastian S. Cocioba
Just in case someone needs to check the integrity of their sucrose. It should be negative in a Benedict’s Test.
Benedict’s Test: Principle, Procedure & Practical Uses
Learn the principle, step-by-step procedure, and results of Benedict’s Test. Discover its uses in detecting reducing sugars in biological and chemical samples.
microbenotes.com
December 28, 2025 at 8:00 PM
Just in case someone needs to check the integrity of their sucrose. It should be negative in a Benedict’s Test.
The good stuff.
December 28, 2025 at 7:39 PM
The good stuff.
What do folks actually learn in a bioengineering major in undergrad? Do they teach good design practices? Deep dive into various parts and the fuzzy spaces between them? Do they teach troubleshooting?
I want to write a curriculum for practical bioengineering bc idk if it exists.
I want to write a curriculum for practical bioengineering bc idk if it exists.
December 27, 2025 at 4:36 PM
What do folks actually learn in a bioengineering major in undergrad? Do they teach good design practices? Deep dive into various parts and the fuzzy spaces between them? Do they teach troubleshooting?
I want to write a curriculum for practical bioengineering bc idk if it exists.
I want to write a curriculum for practical bioengineering bc idk if it exists.
I wish there was a game where I could simulate mixing chemicals in flasks and it have a realistic chemical physics engine to emulate a real world lab, but with no dumbing down of the processes. A real ChemSim. Would play it all day, lol.
Role play an industrial chemist :)
Role play an industrial chemist :)
December 27, 2025 at 3:20 PM
I wish there was a game where I could simulate mixing chemicals in flasks and it have a realistic chemical physics engine to emulate a real world lab, but with no dumbing down of the processes. A real ChemSim. Would play it all day, lol.
Role play an industrial chemist :)
Role play an industrial chemist :)
So delighted my tricot snapdragon seeds are ready for harvest. Let's see if I can stabilize this trait. Would be a very cool unique flower to offer in my shop!
December 27, 2025 at 8:32 AM
So delighted my tricot snapdragon seeds are ready for harvest. Let's see if I can stabilize this trait. Would be a very cool unique flower to offer in my shop!
Poking around various models to see where plant bioengineering hits a biosafety flag. Claude Opus 4.5 immediately says no to general overview. Working with someone at anthropic to help them smooth this out.
December 24, 2025 at 4:19 PM
Poking around various models to see where plant bioengineering hits a biosafety flag. Claude Opus 4.5 immediately says no to general overview. Working with someone at anthropic to help them smooth this out.
Reposted by Sebastian S. Cocioba
