Sebastian S. Cocioba
@atinygreencell.bsky.social
Amateur 🌿 Biologist • Aspiring 🌻 Designer
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
November 11, 2025 at 5:33 AM
I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
November 10, 2025 at 6:20 PM
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
November 10, 2025 at 5:35 PM
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
November 10, 2025 at 3:52 PM
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
November 10, 2025 at 4:17 AM
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.
November 10, 2025 at 2:53 AM
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.
S-stock solution prepped 🤢
November 10, 2025 at 12:40 AM
S-stock solution prepped 🤢
Another reason petunia so good is that it can tell you the pollination worked in 24hrs or less. Flower starts to wilt if pollen took. So convenient for crossing.
November 9, 2025 at 9:14 PM
Another reason petunia so good is that it can tell you the pollination worked in 24hrs or less. Flower starts to wilt if pollen took. So convenient for crossing.
So many varieties out of this mutagenesis run!
November 9, 2025 at 8:57 PM
So many varieties out of this mutagenesis run!
Registering my home lab so I can grow my mutant plants for sale later next year! It's so nice working with non-gm plants since you can just go to market immediately. Gonna try to make some revenue to fund the harder projects I have baking.
Yay, bootstrapped flowers!
Yay, bootstrapped flowers!
November 9, 2025 at 6:28 PM
Registering my home lab so I can grow my mutant plants for sale later next year! It's so nice working with non-gm plants since you can just go to market immediately. Gonna try to make some revenue to fund the harder projects I have baking.
Yay, bootstrapped flowers!
Yay, bootstrapped flowers!
Designed a quick mod to my gene gun's petri dish caddy to hold my 60mm autoclavable bead box dishes. It also supports standard 100mm, with a modest 2mm rise in elevation. Yay, rapid prototyping!
November 9, 2025 at 4:50 PM
Designed a quick mod to my gene gun's petri dish caddy to hold my 60mm autoclavable bead box dishes. It also supports standard 100mm, with a modest 2mm rise in elevation. Yay, rapid prototyping!
Oooh, exciting results! Seems the whiskering and/or (most likely) vortexing caused significant trauma to the leaf disks. Its not the bleach bc disks from same leaf plated immediately are perfectly green. Cooooool, got something to optimize for. Next up is to see if vortex alone.
November 9, 2025 at 3:18 PM
Oooh, exciting results! Seems the whiskering and/or (most likely) vortexing caused significant trauma to the leaf disks. Its not the bleach bc disks from same leaf plated immediately are perfectly green. Cooooool, got something to optimize for. Next up is to see if vortex alone.
I'm gonna throw some tungsten at petunia leaf disks tomorrow! Will document and share. Shooting in pCAMBIA2300-RUBYRED, my standard candle for transformation.
November 9, 2025 at 5:10 AM
I'm gonna throw some tungsten at petunia leaf disks tomorrow! Will document and share. Shooting in pCAMBIA2300-RUBYRED, my standard candle for transformation.
November 9, 2025 at 4:26 AM
How much do y'all pay for syringe filters? I can't really find a supplier selling new sterile PES filters for less than ~$1/filter. Thoughts?
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
November 9, 2025 at 3:40 AM
How much do y'all pay for syringe filters? I can't really find a supplier selling new sterile PES filters for less than ~$1/filter. Thoughts?
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
Ideally 33mm, 0.22um, PES hydrophilic
TYIA!!!
So much gauche disrespect for a very serious medicine. So much spectacle and downplaying. Imagine doing this for a chemotheraputic or last line antibiotic. Psilocybin can rewire neurons. It ***changes*** you. Stop this techbro hype nonsense and respect the chemistry ffs.
November 8, 2025 at 4:36 PM
So much gauche disrespect for a very serious medicine. So much spectacle and downplaying. Imagine doing this for a chemotheraputic or last line antibiotic. Psilocybin can rewire neurons. It ***changes*** you. Stop this techbro hype nonsense and respect the chemistry ffs.
I switched agar to save some cash and the snapdragons hate it, lol. Too dense. Gonna try for 6g/L instead of 7, at pH 5.70.
November 8, 2025 at 4:26 PM
I switched agar to save some cash and the snapdragons hate it, lol. Too dense. Gonna try for 6g/L instead of 7, at pH 5.70.
My mutant tricot is bloomin!!!
November 8, 2025 at 3:37 PM
My mutant tricot is bloomin!!!
Although I respect Mendel, I much prefer a tweezer over a brush. Way more efficient. Just pluck off the anther and dab it on the stigma directly.
November 8, 2025 at 3:20 PM
Although I respect Mendel, I much prefer a tweezer over a brush. Way more efficient. Just pluck off the anther and dab it on the stigma directly.
My lab is a quiet storm of dragons.
November 8, 2025 at 3:52 AM
My lab is a quiet storm of dragons.
Super fast bloom! Currently at 41 days seed to bloom. Trying to shave that down to a flat 30 days over the next few generations. My lovely Alice is an F1 driver.
💚🌺🏎🏁
💚🌺🏎🏁
November 8, 2025 at 2:56 AM
Super fast bloom! Currently at 41 days seed to bloom. Trying to shave that down to a flat 30 days over the next few generations. My lovely Alice is an F1 driver.
💚🌺🏎🏁
💚🌺🏎🏁
Yes! It has some yellow in the gills and on the inside dorsal petal but no red so many chs mutant!
November 8, 2025 at 2:20 AM
Yes! It has some yellow in the gills and on the inside dorsal petal but no red so many chs mutant!
Such gentle creatures
November 8, 2025 at 2:01 AM
Such gentle creatures
I makes another pretty mutant! All the flowers are on the same node or at least extremely compacted internodal lengths.
November 7, 2025 at 11:57 PM
I makes another pretty mutant! All the flowers are on the same node or at least extremely compacted internodal lengths.