Sebastian S. Cocioba
@atinygreencell.bsky.social
Amateur 🌿 Biologist • Aspiring 🌻 Designer
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
Independent Researcher at Binomica Labs
Degreeless Heathen • #diybio • #homelab
Open Lab Notebook: tinyurl.com/ATGCFFE
YouTube Channel: https://youtube.com/@atinygreencell
Pinned
Sebastian's Biotech Bazaar
Designer of fine, hand-crafted, bespoke, and fully open source biotechnology tools for educators and researchers. Plasmids, lab equipment, cell lines, and seeds. An ever-growing collection of high qua...
ATinyGreenCell.com
Excited to announce some new plasmid offerings in my shop at atinygreencell.com! Here's a thread of the current catalog. All proceeds directly support my lab and my continued effort to build useful open-source tools. Feel free to get in touch if you want to know more. Please share! 💚
1/7
1/7
I'm slowly becoming a plant GMO deregulation sherpa, lol. Been neck deep in the repeal documents; that activist lawsuit set plant biotech back a decade and folks entering this industrial space should read the gudience the USDA put out to help. The game is back to Ultra Hard Mode.
snoop dogg wearing sunglasses and a ronin camera
ALT: snoop dogg wearing sunglasses and a ronin camera
media.tenor.com
November 15, 2025 at 12:57 AM
I'm slowly becoming a plant GMO deregulation sherpa, lol. Been neck deep in the repeal documents; that activist lawsuit set plant biotech back a decade and folks entering this industrial space should read the gudience the USDA put out to help. The game is back to Ultra Hard Mode.
I love the industrial atmo and string synergy in Nigel's work. One of my favorite albums for deep work.
November 14, 2025 at 3:55 PM
I love the industrial atmo and string synergy in Nigel's work. One of my favorite albums for deep work.
Oooooh, so it seems the plasmolysis buffer + vortexing, even without any whiskers or binding buffer, seems to be causing some necrosis.
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
November 14, 2025 at 3:12 PM
Oooooh, so it seems the plasmolysis buffer + vortexing, even without any whiskers or binding buffer, seems to be causing some necrosis.
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
MS NEG = MS media, no vortex
MS POS = MS media, yes vortex
PLAS NEG = plasmolysis, no vortex
PLAS POS = plasmolysis, yes vortex
Plot thickens!
Reposted by Sebastian S. Cocioba
Just spent a sesh going over my lab student's PhD app personal statement, and omg how she's grown as a writer and scientist. My heart is absolutely singing for stuff like this.
November 13, 2025 at 3:09 AM
Just spent a sesh going over my lab student's PhD app personal statement, and omg how she's grown as a writer and scientist. My heart is absolutely singing for stuff like this.
Reposted by Sebastian S. Cocioba
NSF is open again!
A few comments:
*Please be patient.
During a shutdown NSF employees cannot open computers or respond to emails.
*Merit review will continue. However panels won’t resume until after Dec 8th.
*POs remain excited and committed to advancing science and the scientific workforce.
A few comments:
*Please be patient.
During a shutdown NSF employees cannot open computers or respond to emails.
*Merit review will continue. However panels won’t resume until after Dec 8th.
*POs remain excited and committed to advancing science and the scientific workforce.
November 13, 2025 at 5:58 PM
NSF is open again!
A few comments:
*Please be patient.
During a shutdown NSF employees cannot open computers or respond to emails.
*Merit review will continue. However panels won’t resume until after Dec 8th.
*POs remain excited and committed to advancing science and the scientific workforce.
A few comments:
*Please be patient.
During a shutdown NSF employees cannot open computers or respond to emails.
*Merit review will continue. However panels won’t resume until after Dec 8th.
*POs remain excited and committed to advancing science and the scientific workforce.
Reposted by Sebastian S. Cocioba
“Bin Chicken” is now published in Nature Methods! It substantially improves genome recovery through rational coassembly 🧬🖥️. Applied to public 🌍 metagenomes, we recovered 24,000 novel species 🦠, including 6 new phyla.
doi.org/10.1038/s415...
@benjwoodcroft.bsky.social @rhysnewell.bsky.social
🧵1/6
doi.org/10.1038/s415...
@benjwoodcroft.bsky.social @rhysnewell.bsky.social
🧵1/6
November 13, 2025 at 10:09 AM
“Bin Chicken” is now published in Nature Methods! It substantially improves genome recovery through rational coassembly 🧬🖥️. Applied to public 🌍 metagenomes, we recovered 24,000 novel species 🦠, including 6 new phyla.
doi.org/10.1038/s415...
@benjwoodcroft.bsky.social @rhysnewell.bsky.social
🧵1/6
doi.org/10.1038/s415...
@benjwoodcroft.bsky.social @rhysnewell.bsky.social
🧵1/6
Precious lil petunia paws
November 14, 2025 at 4:45 AM
Precious lil petunia paws
I got the flower design job at Neoplants !!!! Gonna make very cool plants with da crew again. So very thankful for these folks believing in me, and my crazy ideas. What a huge relief in these so incredibly uncertain times. Gonna have real health insurance for once, lol.
🇫🇷🧬🌺
🇫🇷🧬🌺
November 13, 2025 at 11:20 PM
I got the flower design job at Neoplants !!!! Gonna make very cool plants with da crew again. So very thankful for these folks believing in me, and my crazy ideas. What a huge relief in these so incredibly uncertain times. Gonna have real health insurance for once, lol.
🇫🇷🧬🌺
🇫🇷🧬🌺
Reposted by Sebastian S. Cocioba
🌱 From PNAS: Meristem layers mutate at different rates, with the germline-forming L2 protected and the L1 evolving faster. (Kirk R. Amundson, Mohan P. A. Marimuthu, Oanh Nguyen, Konsam Sarika, Isabelle J. DeMarco, Angelina Phan, Isabelle M. Henry, and Luca Comai)
▶️ www.pnas.org/doi/10.1073/...
▶️ www.pnas.org/doi/10.1073/...
Spatial variation in the mutation rate within the plant shoot apical meristem | PNAS
The potential impact of new mutations on organismal function and evolution depends
on the developmental fate of the affected cells. Stem cells in t...
www.pnas.org
November 13, 2025 at 9:46 PM
OMG MY ACYANIC SNAPDRAGON MUTANT IS SELF COMPATIBLE!!!
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
November 12, 2025 at 8:09 PM
OMG MY ACYANIC SNAPDRAGON MUTANT IS SELF COMPATIBLE!!!
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
All my other ones need to be crossed since they have an s-locus self incompatibility.
THIS ONE DOESN'T????
Reference:
academic.oup.com/mbe/article/...
Carbide Whisker Experiment #2 underway!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
November 12, 2025 at 4:55 PM
Carbide Whisker Experiment #2 underway!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Exploring the effects of plasmolysis and/or vortexing on necrosis in petunia leaf disks. I want to systematically go through the variables and attempt to link cause to the necrosis I saw in the first experiment. 24hrs in and disks healthy!
Blarg, I needed a positive control for my whacky tungsten gene gun experiments so I caved and bought some premade gold. Enough for about 100 shots. Expensive but adds a critical control to my experiments so I'm not literally shooting in the dark. My wallet tho 😭
November 12, 2025 at 12:39 AM
Blarg, I needed a positive control for my whacky tungsten gene gun experiments so I caved and bought some premade gold. Enough for about 100 shots. Expensive but adds a critical control to my experiments so I'm not literally shooting in the dark. My wallet tho 😭
Big scam's afoot. Block and report for clear ToS violation. Fly safe, friends!
November 11, 2025 at 11:51 PM
Big scam's afoot. Block and report for clear ToS violation. Fly safe, friends!
Decent minimalist vortexer with touch activation. $25 on Amazon. Fixed speed but it can sling a 50mL to a full tornado and thats enough for me. Force needed low enough to touch vortex an open eppi tube with one hand (my very specific genegun prep needs). 12v generic barrel jack. Nice
a.co/d/bkrYeEz
a.co/d/bkrYeEz
November 11, 2025 at 11:16 PM
Decent minimalist vortexer with touch activation. $25 on Amazon. Fixed speed but it can sling a 50mL to a full tornado and thats enough for me. Force needed low enough to touch vortex an open eppi tube with one hand (my very specific genegun prep needs). 12v generic barrel jack. Nice
a.co/d/bkrYeEz
a.co/d/bkrYeEz
Crossing blooms is my happy place 💚🌺
November 11, 2025 at 11:04 PM
Crossing blooms is my happy place 💚🌺
Reposted by Sebastian S. Cocioba
Antirrhinum flower shape: unravelling gene expression across developmental axes and boundaries https://www.biorxiv.org/content/10.1101/2025.11.10.687484v1
November 11, 2025 at 5:11 PM
Antirrhinum flower shape: unravelling gene expression across developmental axes and boundaries https://www.biorxiv.org/content/10.1101/2025.11.10.687484v1
Reposted by Sebastian S. Cocioba
I USED AVENGERS FONT IN A RAINBOW FIGURE ONCE!!!
And THREE puns in one sentence.
We really need to have a lot more fun and joy in our scholarly papers!
And THREE puns in one sentence.
We really need to have a lot more fun and joy in our scholarly papers!
November 11, 2025 at 5:30 PM
I USED AVENGERS FONT IN A RAINBOW FIGURE ONCE!!!
And THREE puns in one sentence.
We really need to have a lot more fun and joy in our scholarly papers!
And THREE puns in one sentence.
We really need to have a lot more fun and joy in our scholarly papers!
Some very cheap new old stock rupture disks arrived this morning. A bit too strong for my needs but I can simply lower the petri dish away from the barrel of the gene gun. Its just thick-ass kapton. Time to Cricut my own disks!
November 11, 2025 at 4:42 PM
Some very cheap new old stock rupture disks arrived this morning. A bit too strong for my needs but I can simply lower the petri dish away from the barrel of the gene gun. Its just thick-ass kapton. Time to Cricut my own disks!
I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
November 11, 2025 at 5:33 AM
I love the aesthetic of this process. Very steampunk. I wish there were more models of gene guns and a healthy ecosystem of industrial designs. The DIY ones have charm, but also ample room for really cool innovations in this space, esp now that regulations are back to pre-2019.
Reposted by Sebastian S. Cocioba
Excited to share this perspective article out now! Come read about Streptocarpus and other great model systems!
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration
ABSTRACT. The ability of organisms to replace and regenerate anatomical structures following their loss or damage has piqued the curiosity of biologists for centuries. In addition to Development's ‘Mo...
journals.biologists.com
October 28, 2025 at 1:23 PM
Excited to share this perspective article out now! Come read about Streptocarpus and other great model systems!
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
More extraordinary model systems for regeneration journals.biologists.com/dev/article/...
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
November 10, 2025 at 6:20 PM
trying to be a real human on linkedIn is gonna be an interesting experiment. that site is a unique hellscape so lets see what happens when I post my usual way on there too. prolly gonna backfire spectacularly but worth a try lol
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
November 10, 2025 at 5:35 PM
My breeding ledger is getting elaborate, lol. Link if anyone wants to browse:
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
November 10, 2025 at 3:52 PM
These autoclavable polypropylene fly fishing bead boxes (TidyCrafts) have a convenient injection molding scar dead center which helps me orient leaf tissue for gene gun shots.
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
November 10, 2025 at 4:17 AM
Kapowwwww 💥
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Gene gun go pop and tungsten go zooooom 🧬🔫💥🌱
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.
November 10, 2025 at 2:53 AM
Exploratory shots with RUBY to track distance to target as a variable. Constant pressure (1100psi) and same shot stock.