Grigory Tagiltsev
grigorytagiltsev.bsky.social
Grigory Tagiltsev
@grigorytagiltsev.bsky.social
Currently postdoc @briggsgroup.bsky.social @mpibiochem.bsky.social | PhD @ScheuringLab @weillcornell.bsky.social | #biophysics, #cryEM, #cryoET, #AFM
Pinned
Finally out! Working with this team was a fantastic learning experience. Also I was excited to determine the in situ clathrin structure for this project! That’s my first EM-structure upload to EMDB. Can I tell my mom I am a structural biologist now? 😁 #cryoET
Reposted by Grigory Tagiltsev
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
October 28, 2025 at 3:07 PM
Reposted by Grigory Tagiltsev
Hey #TeamTomo …would you like to try some 🍦ICECREAM!? Our @unibas.ch colleagues over at the @ivandokmanic.bsky.social lab have developed a stunningly effective tool to de-noise and de-wedge your tomograms. The contrast and details will give you brain freeze! 🍨❄️🤯

Please try, we need feedback 🧪🧶🧬🔬
October 23, 2025 at 5:57 PM
Reposted by Grigory Tagiltsev
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 11:13 AM
Reposted by Grigory Tagiltsev
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
The mechanistic basis of cargo selection during Golgi maturation
Resident proteins of the Golgi recycle in vesicles and the protein GOLPH3 enables the COPI vesicle coat to accomplish this.
www.science.org
October 6, 2025 at 11:39 AM
Reposted by Grigory Tagiltsev
Providing order amongst constant traffic: #LMBResearch from Sean Munro (@cellbiol-mrclmb.bsky.social) with John Briggs (@mpibiochem.bsky.social) reveals how GOLPH3 allows COPI vesicles to distinguish between Golgi residents & ER-bound proteins.
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
October 6, 2025 at 8:07 AM
Reposted by Grigory Tagiltsev
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
September 23, 2025 at 5:08 PM
Reposted by Grigory Tagiltsev
A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾
🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture.
buff.ly/j3TSIkn
September 20, 2025 at 7:24 PM
Reposted by Grigory Tagiltsev
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters - Nature Structural & Molecular Biology
Reddy et al. used ancestral protein reconstruction, cryo-electron microscopy and functional assays to elucidate how a secondary active transporter evolved to harness the energy of sodium gradients to ...
go.nature.com
September 12, 2025 at 12:08 AM
Reposted by Grigory Tagiltsev
In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria
www.cell.com/cell-reports...
#cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social
September 14, 2025 at 8:52 AM
Reposted by Grigory Tagiltsev
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Reposted by Grigory Tagiltsev
Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇

For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!

www.mdc-berlin.de/postdocs#t-g...
www.mdc-berlin.de
August 4, 2025 at 10:23 AM
Reposted by Grigory Tagiltsev
Director at Max Planck - a unique position! The Open Call for Expressions of Interest in Max Planck Directorships is open now and can be submitted by the 31st of October 2025. ➡️ mpg.de/directors - Please share the Open Call among potential candidates.
August 1, 2025 at 9:06 AM
Reposted by Grigory Tagiltsev
New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
July 26, 2025 at 3:38 PM
Reposted by Grigory Tagiltsev
And here it is now in print! www.cell.com/cell-reports...

With a front cover splash 😍
July 21, 2025 at 7:15 PM
Reposted by Grigory Tagiltsev
Our entrance into protein design! Inverse folding steered by external sources of information, and for multiple conformations. By the amazing @kaiyi94.bsky.social and @kjamali.bsky.social!
Download our code and try it yourself. 🥳
Excited to share our paper at #ICML: All-atom inverse protein folding through discrete flow matching with @kjamali.bsky.social and @sjorsscheres.bsky.social : openreview.net/forum?id=8tQdw…. If you are at ICML, let’s connect and talk generative models&protein design!
OpenReview
Promoting openness in scientific communication and the peer-review process
https://openreview.net/forum?id=8tQdw…
July 15, 2025 at 7:18 AM
Reposted by Grigory Tagiltsev
This is cool. CryoEM showing COPI coat containing GOLPH3, together with functional data suggests the mechanism for cargo retention/transport.

The mechanistic basis of cargo selection during Golgi maturation

Taylor, Zubkov, Ciazynska et al.

doi.org/10.1101/2025...
The mechanistic basis of cargo selection during Golgi maturation
The multiple cisternae of the Golgi apparatus contain resident membrane proteins crucial for lipid and protein glycosylation. How Golgi residents remain in their designated compartments despite a cons...
doi.org
June 27, 2025 at 8:16 AM
Reposted by Grigory Tagiltsev
Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi

Out in @pnas.org

Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social
@mrclmb.bsky.social @hopitalnecker.bsky.social

www.pnas.org/doi/10.1073/...
June 23, 2025 at 10:26 AM
Reposted by Grigory Tagiltsev
Applications close this Friday, 20th June.
June 17, 2025 at 8:39 AM
Reposted by Grigory Tagiltsev
Congratulations! The #ShawPrize in Life Science & Medicine 2025 is awarded to Wolfgang Baumeister for his pioneering development & use of cryo-ET.

❕https://www.shawprize.org/laureates/2025-life-science-medicine/

#CryoEM #cryoET #Award
May 27, 2025 at 2:15 PM
Reposted by Grigory Tagiltsev
We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation.

Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.

cryotud.github.io/locscale/
May 22, 2025 at 10:10 AM
Reposted by Grigory Tagiltsev
Super happy to announce that together with @stefvass.bsky.social , we will have the honor to join 1st authors Florian Wernert and @moparthisk.bsky.social to present our recent work (www.science.org/doi/10.1126/...) to the French Academy of Sciences @academiesciences.bsky.social!
The actin-spectrin submembrane scaffold restricts endocytosis along proximal axons
Clathrin-mediated endocytosis has characteristic features in neuronal dendrites and presynapses, but how membrane proteins are internalized along the axon shaft remains unclear. We focused on clathrin...
www.science.org
May 23, 2025 at 2:42 PM
Reposted by Grigory Tagiltsev
Congratulations to LMB Group Leaders Leo James, Greg Jefferis & Marta Zlatic who have been elected Fellows of the @royalsociety.org!

Congratulations also to the #LMBAlumni joining them: John Briggs, Graham Hatfull & Baljit Khakh.

Read more: www2.mrc-lmb.cam.ac.uk/leo-james-gr...

#LMBNews
May 20, 2025 at 9:09 AM
Reposted by Grigory Tagiltsev
John Briggs has been elected to the Fellowship of the Royal Society, the UK’s national academy of sciences. Congratulations to him and the other outstanding researchers from across the world!

❕https://royalsociety.org/news/2025/05/new-fellows/

@briggsgroup.bsky.social ‪@royalsociety.org
May 20, 2025 at 12:48 PM
Reposted by Grigory Tagiltsev
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!

tomoguide.github.io

Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 6, 2025 at 4:27 PM
Reposted by Grigory Tagiltsev
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
April 25, 2025 at 7:28 AM