Callie Glynn
@glynnca.bsky.social
Senior postdoc at the Rosalind Franklin Institute, Oxfordshire UK. Structural Biology. Interests cryo-EM, cryoET, amyloid structure, tau, prion, iced tea. (She/her)
Reposted by Callie Glynn
Visualizing influenza A virus assembly by in situ cryo-electron tomography
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Visualizing influenza A virus assembly by in situ cryo-electron tomography - Nature Communications
Influenza A virus must package eight separate genomic segments, called viral ribonucleoproteins (vRNPs). Using in situ cryo-electron tomography, the authors visualize how vRNPs are clustered on cell m...
www.nature.com
October 23, 2025 at 2:01 PM
Visualizing influenza A virus assembly by in situ cryo-electron tomography
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Callie Glynn
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
October 16, 2025 at 10:34 PM
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
Reposted by Callie Glynn
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
October 16, 2025 at 6:23 AM
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Reposted by Callie Glynn
Excited to share our preprint on cytoskeletal organization in astrocytes!
biorxiv.org/content/10.110…
Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.
Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓
1/x
biorxiv.org/content/10.110…
Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.
Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓
1/x
https://biorxiv.org/content/10.110…
October 13, 2025 at 8:44 PM
Excited to share our preprint on cytoskeletal organization in astrocytes!
biorxiv.org/content/10.110…
Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.
Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓
1/x
biorxiv.org/content/10.110…
Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.
Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓
1/x
Reposted by Callie Glynn
Huntingtin binds and bundles F-actin!
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
September 25, 2025 at 3:01 PM
Huntingtin binds and bundles F-actin!
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery.
We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead!
#TeamTomo #CryoET #HTT #actin
doi.org/10.1126/scia...
Reposted by Callie Glynn
An international research team, including the @schurlab.bsky.social at ISTA and partners from the University of Grenoble Alpes, KAIST, @sorbonne-universite.fr, Hanyang University, and EPFL, just released a new paper in Science Advances.
September 22, 2025 at 1:57 PM
An international research team, including the @schurlab.bsky.social at ISTA and partners from the University of Grenoble Alpes, KAIST, @sorbonne-universite.fr, Hanyang University, and EPFL, just released a new paper in Science Advances.
Reposted by Callie Glynn
Structure of ATTRv-F64S fibrils isolated from skin tissue of a living patient www.biorxiv.org/content/10.1101/2025.07.29.667442v1 #cryoem
July 31, 2025 at 9:16 AM
Structure of ATTRv-F64S fibrils isolated from skin tissue of a living patient www.biorxiv.org/content/10.1101/2025.07.29.667442v1 #cryoem
Reposted by Callie Glynn
Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV
Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…
www.sciencedirect.com
July 30, 2025 at 9:50 PM
Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Callie Glynn
New preprint: In situ Architecture of #Plasmodesmata.
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
July 26, 2025 at 3:38 PM
New preprint: In situ Architecture of #Plasmodesmata.
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.
🔗 doi.org/10.1101/2025...
🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
Reposted by Callie Glynn
July 21, 2025 at 7:15 PM
Reposted by Callie Glynn
Delighted to see our work in press! 🙌🔬🧠
Thanks to @glynnca.bsky.social, @michaelgrange.bsky.social and the rest of the fabulous co-authors for all your contributions.
Excited to see where in tissue structural cell biology goes next 👀!
#liftlaughlove #teamtomo #whopper
Thanks to @glynnca.bsky.social, @michaelgrange.bsky.social and the rest of the fabulous co-authors for all your contributions.
Excited to see where in tissue structural cell biology goes next 👀!
#liftlaughlove #teamtomo #whopper
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
June 19, 2025 at 9:23 AM
Delighted to see our work in press! 🙌🔬🧠
Thanks to @glynnca.bsky.social, @michaelgrange.bsky.social and the rest of the fabulous co-authors for all your contributions.
Excited to see where in tissue structural cell biology goes next 👀!
#liftlaughlove #teamtomo #whopper
Thanks to @glynnca.bsky.social, @michaelgrange.bsky.social and the rest of the fabulous co-authors for all your contributions.
Excited to see where in tissue structural cell biology goes next 👀!
#liftlaughlove #teamtomo #whopper
Reposted by Callie Glynn
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
June 19, 2025 at 8:58 AM
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Reposted by Callie Glynn
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
June 17, 2025 at 10:59 AM
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Reposted by Callie Glynn
CoVET: Cryo-Electron Tomography guided by optical electrophysiology www.biorxiv.org/content/10.1101/2025.05.14.653951v1 #cryoem
May 18, 2025 at 7:10 AM
CoVET: Cryo-Electron Tomography guided by optical electrophysiology www.biorxiv.org/content/10.1101/2025.05.14.653951v1 #cryoem
Reposted by Callie Glynn
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
April 25, 2025 at 7:28 AM
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
Reposted by Callie Glynn
New lab preprint! Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy (Cryo-ExM). A fantastic collaboration with Dr. Benita Wolf. Congratulations to all authors, especially Florent Lemaitre, for leading this amazing work! www.biorxiv.org/content/10.1...
April 23, 2025 at 2:04 PM
New lab preprint! Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy (Cryo-ExM). A fantastic collaboration with Dr. Benita Wolf. Congratulations to all authors, especially Florent Lemaitre, for leading this amazing work! www.biorxiv.org/content/10.1...
Reposted by Callie Glynn
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is
radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
April 23, 2025 at 4:23 AM
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reposted by Callie Glynn
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
April 11, 2025 at 9:04 AM
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
Reposted by Callie Glynn
Exciting News! We’re thrilled to announce the first Functional Amyloid Meeting at DESY!
Join us for a deep dive into the latest breakthroughs in functional fibrils, with a spotlight on amyloid molecular and cellular mechanisms and their biomedical applications.
landau-cssb.de/meeting-of-f...
Join us for a deep dive into the latest breakthroughs in functional fibrils, with a spotlight on amyloid molecular and cellular mechanisms and their biomedical applications.
landau-cssb.de/meeting-of-f...
March 20, 2025 at 7:56 AM
Exciting News! We’re thrilled to announce the first Functional Amyloid Meeting at DESY!
Join us for a deep dive into the latest breakthroughs in functional fibrils, with a spotlight on amyloid molecular and cellular mechanisms and their biomedical applications.
landau-cssb.de/meeting-of-f...
Join us for a deep dive into the latest breakthroughs in functional fibrils, with a spotlight on amyloid molecular and cellular mechanisms and their biomedical applications.
landau-cssb.de/meeting-of-f...
Reposted by Callie Glynn
Happy to share that the final version of record for our (
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
March 7, 2025 at 12:32 AM
Happy to share that the final version of record for our (
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
@nanigrotjahn.bsky.social) #teamtomo mitochondrial cotranslation story is online at @jcb.org! We combined STA with surface morphometrics to learn how ribosomes bind to and alter mitochondrial membranes in yeast.
rupress.org/jcb/article/...
Reposted by Callie Glynn
New Title Alert: napari-tomoslice- a plugin for napari to aid in tomogram visualisation and annotation.
Learn more here: https://buff.ly/3Eq8cGP
#SBGrid #Software #StructuralBiology
Learn more here: https://buff.ly/3Eq8cGP
#SBGrid #Software #StructuralBiology
GitHub - alisterburt/napari-tomoslice: tomogram visualisation and annotation in napari
tomogram visualisation and annotation in napari. Contribute to alisterburt/napari-tomoslice development by creating an account on GitHub.
buff.ly
February 23, 2025 at 4:31 PM
New Title Alert: napari-tomoslice- a plugin for napari to aid in tomogram visualisation and annotation.
Learn more here: https://buff.ly/3Eq8cGP
#SBGrid #Software #StructuralBiology
Learn more here: https://buff.ly/3Eq8cGP
#SBGrid #Software #StructuralBiology
Reposted by Callie Glynn
Interested in learning to process cryo-ET data in the latest version of Warp? I'll be running a workshop on April 22nd in central London, UK
details+registration: forms.gle/FLDr3ZWtVGXs...
Hope to see you there!
details+registration: forms.gle/FLDr3ZWtVGXs...
Hope to see you there!
February 11, 2025 at 9:59 PM
Interested in learning to process cryo-ET data in the latest version of Warp? I'll be running a workshop on April 22nd in central London, UK
details+registration: forms.gle/FLDr3ZWtVGXs...
Hope to see you there!
details+registration: forms.gle/FLDr3ZWtVGXs...
Hope to see you there!
Reposted by Callie Glynn
It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.
February 8, 2025 at 9:21 PM
It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.
Reposted by Callie Glynn
Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:
February 6, 2025 at 11:02 AM
Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:
Reposted by Callie Glynn
Looking forward to talking about MemBrain-seg tomorrow and giving a sneak peek into other MemBrain v2 functionalities 👀🚀
On January 14th, our software webinar will feature @lorenzlamm.bsky.social , MUDS - Munich School for Data Science Fellow at Boehringer Ingelheim, on MemBrain v2.
MemBrain v2
Tuesday, January 14, 2025 at 12:00pm EDT
Lorenz Lamm
Register here: https://buff.ly/36OEPwR
#structuralbiology #webinars
MemBrain v2
Tuesday, January 14, 2025 at 12:00pm EDT
Lorenz Lamm
Register here: https://buff.ly/36OEPwR
#structuralbiology #webinars
Webinars
The SBGrid Consortium is an innovative global research computing group operated out of Harvard Medical School. SBGrid provides the global structural biology community with support for research…
buff.ly
January 13, 2025 at 5:33 PM
Looking forward to talking about MemBrain-seg tomorrow and giving a sneak peek into other MemBrain v2 functionalities 👀🚀