Claudio Alfieri
@claudioalfieri.bsky.social
Reposted by Claudio Alfieri
Our lab @mrclmb.bsky.social in Cambridge is seeking a passionate PhD student to explore transcription-coupled splicing! Join our team for an exciting project to understand human gene regulation 🧬 Feel free to get in touch directly #PhD #cryoEM #biochemistry
www2.mrc-lmb.cam.ac.uk/students/int...
www2.mrc-lmb.cam.ac.uk/students/int...
October 23, 2025 at 10:32 AM
Our lab @mrclmb.bsky.social in Cambridge is seeking a passionate PhD student to explore transcription-coupled splicing! Join our team for an exciting project to understand human gene regulation 🧬 Feel free to get in touch directly #PhD #cryoEM #biochemistry
www2.mrc-lmb.cam.ac.uk/students/int...
www2.mrc-lmb.cam.ac.uk/students/int...
Reposted by Claudio Alfieri
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
October 22, 2025 at 4:41 PM
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Reposted by Claudio Alfieri
Interactions with the nucleosome’s acidic patch and DNA entry/exit site give the chromosomal passenger complex CPC a non-catalytic role in stabilizing #centromeric chromatin
#cryoEM and biochemical, cellular data by AA Jeyaprakash and coworkers
www.embopress.org/doi/full/10....
#cryoEM and biochemical, cellular data by AA Jeyaprakash and coworkers
www.embopress.org/doi/full/10....
October 27, 2025 at 2:58 PM
Interactions with the nucleosome’s acidic patch and DNA entry/exit site give the chromosomal passenger complex CPC a non-catalytic role in stabilizing #centromeric chromatin
#cryoEM and biochemical, cellular data by AA Jeyaprakash and coworkers
www.embopress.org/doi/full/10....
#cryoEM and biochemical, cellular data by AA Jeyaprakash and coworkers
www.embopress.org/doi/full/10....
Reposted by Claudio Alfieri
`Mix-it-up': accessible time-resolved cryo-EM on the millisecond timescale pubmed.ncbi.nlm.nih.gov/41140236/ #cryoem
October 29, 2025 at 1:35 AM
`Mix-it-up': accessible time-resolved cryo-EM on the millisecond timescale pubmed.ncbi.nlm.nih.gov/41140236/ #cryoem
Reposted by Claudio Alfieri
EMBL scientists have new detailed info about Asgard archaea DNA structures offering important clues to more complex cell evolution.
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
October 29, 2025 at 8:29 AM
EMBL scientists have new detailed info about Asgard archaea DNA structures offering important clues to more complex cell evolution.
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
With #cryoEM, scientists found both open and closed chomatin structures with a possible role in regulating chromatin.
doi.org/10.1016/j.mo...
Reposted by Claudio Alfieri
Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin-specific chaperones pubmed.ncbi.nlm.nih.gov/41166473/ #cryoem
October 31, 2025 at 2:22 PM
Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin-specific chaperones pubmed.ncbi.nlm.nih.gov/41166473/ #cryoem
Reposted by Claudio Alfieri
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
October 30, 2025 at 1:33 PM
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Reposted by Claudio Alfieri
I am excited to share our new preprint on our structural and biochemical analyses of human methionine synthase (MTR). This was the hard work of Douglas Ferreira who carried out this research as a significant part of his PhD. #CryoEM www.biorxiv.org/content/10.1...
Structural insights into cobalamin loading and reactivation of human methionine synthase
Human methionine synthase (MTR) is an essential enzyme of one carbon metabolism. Consisting of a catalytic N-half and a cobalamin binding C-half, MTR utilises this intricate organometallic cofactor in...
www.biorxiv.org
November 11, 2025 at 12:48 PM
I am excited to share our new preprint on our structural and biochemical analyses of human methionine synthase (MTR). This was the hard work of Douglas Ferreira who carried out this research as a significant part of his PhD. #CryoEM www.biorxiv.org/content/10.1...
Amazing work from my neighbour's lab! Many congrats to all the people involved! 👏👏👏👏
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
www.science.org
October 17, 2025 at 5:54 PM
Amazing work from my neighbour's lab! Many congrats to all the people involved! 👏👏👏👏
Reposted by Claudio Alfieri
Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures. #CryoEM
September 21, 2025 at 3:02 PM
Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures. #CryoEM
Reposted by Claudio Alfieri
This is super cool and great investigative work! Thanks Oli and co! #smallparticles #cryoEM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 9:30 PM
This is super cool and great investigative work! Thanks Oli and co! #smallparticles #cryoEM
Reposted by Claudio Alfieri
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications
Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...
www.nature.com
August 15, 2025 at 9:26 PM
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Claudio Alfieri
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
May 26, 2025 at 4:56 PM
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
Reposted by Claudio Alfieri
Our integrative approach using #MD and #cryoEM data to construct structural ensembles of #RNA just published on @natcomms.bsky.social doi.org/10.1038/s414... Lead by Elisa Posani, with @magistratolab.bsky.social @bonomimax.bsky.social @pjanos.bsky.social @navtejtoor.bsky.social and Daniel Haack 🎉
May 16, 2025 at 9:16 AM
Our integrative approach using #MD and #cryoEM data to construct structural ensembles of #RNA just published on @natcomms.bsky.social doi.org/10.1038/s414... Lead by Elisa Posani, with @magistratolab.bsky.social @bonomimax.bsky.social @pjanos.bsky.social @navtejtoor.bsky.social and Daniel Haack 🎉
Reposted by Claudio Alfieri
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...
@mpi-bio-fml.bsky.social
@mpi-bio-fml.bsky.social
May 12, 2025 at 9:17 AM
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...
@mpi-bio-fml.bsky.social
@mpi-bio-fml.bsky.social
Reposted by Claudio Alfieri
We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵
Determining the effects of pseudouridine incorporation on human tRNAs | The EMBO Journal
imageimagetRNAs harbour a complex set of modifications which are important for their function.
Here, single particle cryo-EM, biophysics, and computational analyses of the effects
of pseudouridine inc...
www.embopress.org
April 30, 2025 at 4:12 AM
We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵
Reposted by Claudio Alfieri
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
April 11, 2025 at 9:04 AM
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
Reposted by Claudio Alfieri
#CryoSPARC v4.7 is out! 🚀
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates
#cryoEM
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates
#cryoEM
April 9, 2025 at 2:14 PM
#CryoSPARC v4.7 is out! 🚀
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates
#cryoEM
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates
#cryoEM
Reposted by Claudio Alfieri
🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
March 28, 2025 at 3:44 PM
🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM
Reposted by Claudio Alfieri
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
March 24, 2025 at 3:56 PM
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
Reposted by Claudio Alfieri
How does MntR function as both repressor and activator with different Mn(II) sensitivity and cooperativity?
Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky #ChemSky #cryoEM
www.nature.com/articles/s41...
Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky #ChemSky #cryoEM
www.nature.com/articles/s41...
Structural basis for transcription activation through cooperative recruitment of MntR - Nature Communications
MntR is a protein that can repress the expression of Mn2+ uptake transporters and activate the expression of efflux proteins. Here, the authors use cryo-EM and other biochemical approaches to establis...
www.nature.com
March 5, 2025 at 3:48 PM
How does MntR function as both repressor and activator with different Mn(II) sensitivity and cooperativity?
Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky #ChemSky #cryoEM
www.nature.com/articles/s41...
Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky #ChemSky #cryoEM
www.nature.com/articles/s41...
Reposted by Claudio Alfieri
Sad to know about the passing of Béla Novák. This review from Tyson, Chen and Novak was such a revelation for me as an introduction to control theory in biological systems. A classic that will remain relevant for many years to come. www.sciencedirect.com/science/arti...
February 25, 2025 at 6:30 PM
Sad to know about the passing of Béla Novák. This review from Tyson, Chen and Novak was such a revelation for me as an introduction to control theory in biological systems. A classic that will remain relevant for many years to come. www.sciencedirect.com/science/arti...
Reposted by Claudio Alfieri
📢 We’re looking for a new Team Leader!
@enasequence.bsky.social is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources.
Interested?👇
@enasequence.bsky.social is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources.
Interested?👇
February 17, 2025 at 11:07 AM
📢 We’re looking for a new Team Leader!
@enasequence.bsky.social is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources.
Interested?👇
@enasequence.bsky.social is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources.
Interested?👇
Reposted by Claudio Alfieri
Today in @nature.com we share our back-to-back stories with Ning Zheng’s lab revealing chemical-genetic convergence between a molecular glue degrader & E3 ligase cancer mutations. 1/5
February 12, 2025 at 4:20 PM
Today in @nature.com we share our back-to-back stories with Ning Zheng’s lab revealing chemical-genetic convergence between a molecular glue degrader & E3 ligase cancer mutations. 1/5
Reposted by Claudio Alfieri
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 12, 2025 at 6:28 AM
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...