Matthew McCullen
@nkcell.bsky.social
Reposted by Matthew McCullen
BIG NEWS!
The Innate Lymphoid Cell (ILC) 6 Meeting (ILC6) will take place on June 2nd-4th, 2026, in Vancouver!
Keynotes include:
Andrew McKenzie, Kazuyo Moro.
ilc6vancouver.com
#ILC6 #ILC6Vancouver #2026ILC6
(1/3)
The Innate Lymphoid Cell (ILC) 6 Meeting (ILC6) will take place on June 2nd-4th, 2026, in Vancouver!
Keynotes include:
Andrew McKenzie, Kazuyo Moro.
ilc6vancouver.com
#ILC6 #ILC6Vancouver #2026ILC6
(1/3)
November 3, 2025 at 5:58 PM
BIG NEWS!
The Innate Lymphoid Cell (ILC) 6 Meeting (ILC6) will take place on June 2nd-4th, 2026, in Vancouver!
Keynotes include:
Andrew McKenzie, Kazuyo Moro.
ilc6vancouver.com
#ILC6 #ILC6Vancouver #2026ILC6
(1/3)
The Innate Lymphoid Cell (ILC) 6 Meeting (ILC6) will take place on June 2nd-4th, 2026, in Vancouver!
Keynotes include:
Andrew McKenzie, Kazuyo Moro.
ilc6vancouver.com
#ILC6 #ILC6Vancouver #2026ILC6
(1/3)
Reposted by Matthew McCullen
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
October 28, 2025 at 8:06 AM
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Reposted by Matthew McCullen
Happy to share new work from #theonlylabever, lead by the phenomenal @sltai.bsky.social - who developed a tool to study the biology of ILC3s, identifying their role in controlling CSF2-dependent myeloid cells. 🤗
@mucosalimmunol.bsky.social @societymucosalimm.bsky.social
OA: doi.org/10.1016/j.mu...
@mucosalimmunol.bsky.social @societymucosalimm.bsky.social
OA: doi.org/10.1016/j.mu...
October 15, 2025 at 5:11 AM
Happy to share new work from #theonlylabever, lead by the phenomenal @sltai.bsky.social - who developed a tool to study the biology of ILC3s, identifying their role in controlling CSF2-dependent myeloid cells. 🤗
@mucosalimmunol.bsky.social @societymucosalimm.bsky.social
OA: doi.org/10.1016/j.mu...
@mucosalimmunol.bsky.social @societymucosalimm.bsky.social
OA: doi.org/10.1016/j.mu...
Reposted by Matthew McCullen
Asthma is a chronic disease characterized by acute, intermittent, recurrent episodes of airway inflammation. A decade of work developing the tools, techniques and collaborations needed to figure out how CD4+ T cells in the lungs propagate disease can be found here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Tertiary lymphoid structures support the development of allergen-specific progenitor CD4+ T cells
Tissue-resident memory CD4+ T cells (TRM) are key sentinels of the adaptive immune response that provide a rapid, robust inflammatory response upon reactivation in non-lymphoid tissues. While CD4+ TRM...
www.biorxiv.org
October 10, 2025 at 7:22 PM
Asthma is a chronic disease characterized by acute, intermittent, recurrent episodes of airway inflammation. A decade of work developing the tools, techniques and collaborations needed to figure out how CD4+ T cells in the lungs propagate disease can be found here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Matthew McCullen
We are currently in the process of recruiting a technician. 🥼🐭🔬
If you know anyone with enthusiasm to learn new skills and stamina to hear me go on and on about IFNs, please have them reach out to me for more details!
If you know anyone with enthusiasm to learn new skills and stamina to hear me go on and on about IFNs, please have them reach out to me for more details!
October 2, 2025 at 5:41 PM
We are currently in the process of recruiting a technician. 🥼🐭🔬
If you know anyone with enthusiasm to learn new skills and stamina to hear me go on and on about IFNs, please have them reach out to me for more details!
If you know anyone with enthusiasm to learn new skills and stamina to hear me go on and on about IFNs, please have them reach out to me for more details!
Reposted by Matthew McCullen
STAT3 operates as an inflammation-dependent transcriptional switch https://www.biorxiv.org/content/10.1101/2025.09.26.678857v1
September 29, 2025 at 3:16 AM
STAT3 operates as an inflammation-dependent transcriptional switch https://www.biorxiv.org/content/10.1101/2025.09.26.678857v1
Reposted by Matthew McCullen
Dear friends,
As have become a habit, I mark each #autumnequinox by sharing a small poem by Xin Qiji. I also start my translation cycle of Chinese and Japanese poems and prose that mark the twenty-four lunisolar micro-seasons. Translation notes blogged here:
#everynightapoem
As have become a habit, I mark each #autumnequinox by sharing a small poem by Xin Qiji. I also start my translation cycle of Chinese and Japanese poems and prose that mark the twenty-four lunisolar micro-seasons. Translation notes blogged here:
#everynightapoem
September 24, 2025 at 1:47 AM
Dear friends,
As have become a habit, I mark each #autumnequinox by sharing a small poem by Xin Qiji. I also start my translation cycle of Chinese and Japanese poems and prose that mark the twenty-four lunisolar micro-seasons. Translation notes blogged here:
#everynightapoem
As have become a habit, I mark each #autumnequinox by sharing a small poem by Xin Qiji. I also start my translation cycle of Chinese and Japanese poems and prose that mark the twenty-four lunisolar micro-seasons. Translation notes blogged here:
#everynightapoem
Reposted by Matthew McCullen
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com
September 19, 2025 at 2:39 PM
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Matthew McCullen
bioRxiv partners with Dryad to enable easier, better linking to curated datasets connect.biorxiv.org/news/2025/09...
Dryad partnership brings easier data sharing to bioRxiv
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
connect.biorxiv.org
September 18, 2025 at 7:43 PM
bioRxiv partners with Dryad to enable easier, better linking to curated datasets connect.biorxiv.org/news/2025/09...
Reposted by Matthew McCullen
Heads up: ignore samtools dot org, similarly minimap2 dot com and likely others. It's owned by a known phishing site and while the binaries they offer look valid currently (but note they may be serving us different binaries to others), that could change.
Ie: it's not us (Samtools team)! Be warned
Ie: it's not us (Samtools team)! Be warned
September 15, 2025 at 8:40 AM
Heads up: ignore samtools dot org, similarly minimap2 dot com and likely others. It's owned by a known phishing site and while the binaries they offer look valid currently (but note they may be serving us different binaries to others), that could change.
Ie: it's not us (Samtools team)! Be warned
Ie: it's not us (Samtools team)! Be warned
Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis doi.org/10.1038/s415...
Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis - Nature Immunology
Erlich et al. show that soluble LTα and membrane-bound LTα1β2 lymphotoxins expressed by B cells play distinct roles to attenuate the pathology observed in ileitis.
doi.org
September 8, 2025 at 3:52 PM
Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis doi.org/10.1038/s415...
Reposted by Matthew McCullen
IL-25-induced memory type 2 innate lymphoid cells enforce mucosal immunity
Cell www.cell.com/cell/fulltex...
Cell www.cell.com/cell/fulltex...
IL-25-induced memory type 2 innate lymphoid cells enforce mucosal immunity
IL-25 treatment induces persistent adaptation of the small intestine, which is maintained
by ILC2s with functional and epigenetic qualities of immune memory. Effector-memory
ILC2s arise in multiple ti...
www.cell.com
September 5, 2025 at 8:01 PM
IL-25-induced memory type 2 innate lymphoid cells enforce mucosal immunity
Cell www.cell.com/cell/fulltex...
Cell www.cell.com/cell/fulltex...
Reposted by Matthew McCullen
🧵How to write and manage your first research budgets
The point of funding is to convert it into quality research. A well-spent research budget should fund the idea it was raised on, plus revision experiments, plus preliminary data for the next grant. So you need to spend, while avoiding waste.
The point of funding is to convert it into quality research. A well-spent research budget should fund the idea it was raised on, plus revision experiments, plus preliminary data for the next grant. So you need to spend, while avoiding waste.
September 3, 2025 at 4:03 PM
🧵How to write and manage your first research budgets
The point of funding is to convert it into quality research. A well-spent research budget should fund the idea it was raised on, plus revision experiments, plus preliminary data for the next grant. So you need to spend, while avoiding waste.
The point of funding is to convert it into quality research. A well-spent research budget should fund the idea it was raised on, plus revision experiments, plus preliminary data for the next grant. So you need to spend, while avoiding waste.
Reposted by Matthew McCullen
I've got a PSA to the T cell receptor researchers of #immunosky to let you know about a new paper we've just got out: doi.org/10.1016/j.im...
This covers how our field is handling the sets of germline #TCR genes used in our research. Maybe not the most glamorous topic, but it’s kind of a big deal…
This covers how our field is handling the sets of germline #TCR genes used in our research. Maybe not the most glamorous topic, but it’s kind of a big deal…
September 1, 2025 at 3:18 PM
I've got a PSA to the T cell receptor researchers of #immunosky to let you know about a new paper we've just got out: doi.org/10.1016/j.im...
This covers how our field is handling the sets of germline #TCR genes used in our research. Maybe not the most glamorous topic, but it’s kind of a big deal…
This covers how our field is handling the sets of germline #TCR genes used in our research. Maybe not the most glamorous topic, but it’s kind of a big deal…
Reposted by Matthew McCullen
Targeting LAIR1-mediated immunosuppression adds a new weapon to our immunotherapy arsenal: buff.ly/aJxzdS4
Ezri P. Perrin, Hannah K. Dorando & Jacqueline E. Payton provide a Commentary on Tao et al. (buff.ly/uaGEIUb)
Ezri P. Perrin, Hannah K. Dorando & Jacqueline E. Payton provide a Commentary on Tao et al. (buff.ly/uaGEIUb)
August 29, 2025 at 1:20 PM
Targeting LAIR1-mediated immunosuppression adds a new weapon to our immunotherapy arsenal: buff.ly/aJxzdS4
Ezri P. Perrin, Hannah K. Dorando & Jacqueline E. Payton provide a Commentary on Tao et al. (buff.ly/uaGEIUb)
Ezri P. Perrin, Hannah K. Dorando & Jacqueline E. Payton provide a Commentary on Tao et al. (buff.ly/uaGEIUb)
Reposted by Matthew McCullen
Meanwhile in scientific news - our latest paper.
NKG2A-mediated immune modulation of natural killer cells by Staphylococcus aureus
Abstract. Natural killer (NK) cells are specialized lymphocytes that help protect against viruses and cancer. However, in the context of bacterial infectio
academic.oup.com
August 8, 2025 at 10:26 AM
Meanwhile in scientific news - our latest paper.
Reposted by Matthew McCullen
In his lecture on “The Generation and Modification of Lymphocyte Antigen Receptor Genes,” Eugene Oltz, @ohiostatemed.bsky.social, described how chromatin accessibility controls where V(D)J recombination occurs during immune cell development. #AAIcourses
July 28, 2025 at 7:32 PM
In his lecture on “The Generation and Modification of Lymphocyte Antigen Receptor Genes,” Eugene Oltz, @ohiostatemed.bsky.social, described how chromatin accessibility controls where V(D)J recombination occurs during immune cell development. #AAIcourses
Reposted by Matthew McCullen
🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
July 28, 2025 at 9:10 AM
🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Reposted by Matthew McCullen
Liu et al., 2025. An automated ATAC-seq method reveals sequence determinants of transcription factor dose response in the open chromatin www.biorxiv.org/content/10.1...
▶️RoboATAC - automated ATAC-seq
▶️Deep learning models: motif orientation, spacing, flanking bases
▶️Nucleosome positioning analysis
▶️RoboATAC - automated ATAC-seq
▶️Deep learning models: motif orientation, spacing, flanking bases
▶️Nucleosome positioning analysis
July 27, 2025 at 12:31 PM
Liu et al., 2025. An automated ATAC-seq method reveals sequence determinants of transcription factor dose response in the open chromatin www.biorxiv.org/content/10.1...
▶️RoboATAC - automated ATAC-seq
▶️Deep learning models: motif orientation, spacing, flanking bases
▶️Nucleosome positioning analysis
▶️RoboATAC - automated ATAC-seq
▶️Deep learning models: motif orientation, spacing, flanking bases
▶️Nucleosome positioning analysis
Reposted by Matthew McCullen
Shearwater is crowdfunding a new album, definitely art worth contributing to. www.indiegogo.com/projects/a-n...
A New Shearwater Album
Help beloved indie-rock band Shearwater produce, distribute, and publicize a new LP. | Check out 'A New Shearwater Album' on Indiegogo.
www.indiegogo.com
July 8, 2025 at 11:30 PM
Shearwater is crowdfunding a new album, definitely art worth contributing to. www.indiegogo.com/projects/a-n...
Reposted by Matthew McCullen
🚨 New #preprint alert!
We're excited to share the latest work from the brilliant Sora Yoon: "Inference of multi-enhancer interactions in T lymphocytes using Hi-Cociety"🔗 www.biorxiv.org/content/10.1...
We're excited to share the latest work from the brilliant Sora Yoon: "Inference of multi-enhancer interactions in T lymphocytes using Hi-Cociety"🔗 www.biorxiv.org/content/10.1...
June 26, 2025 at 3:43 PM
🚨 New #preprint alert!
We're excited to share the latest work from the brilliant Sora Yoon: "Inference of multi-enhancer interactions in T lymphocytes using Hi-Cociety"🔗 www.biorxiv.org/content/10.1...
We're excited to share the latest work from the brilliant Sora Yoon: "Inference of multi-enhancer interactions in T lymphocytes using Hi-Cociety"🔗 www.biorxiv.org/content/10.1...
Reposted by Matthew McCullen
Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8+ T cells https://www.biorxiv.org/content/10.1101/2025.06.11.659122v1
June 16, 2025 at 8:16 PM
Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8+ T cells https://www.biorxiv.org/content/10.1101/2025.06.11.659122v1
Reposted by Matthew McCullen
New additions in fellowships, industry, and academic jobs! If you've recently gotten a fellowship or a job (research, industry, PUI) please consider contributing your application materials. It's a helpful resource for those on the job market! github.com/RILAB/statem...
GitHub - RILAB/statements: Successful Job Applications and Grants
Successful Job Applications and Grants. Contribute to RILAB/statements development by creating an account on GitHub.
github.com
June 13, 2025 at 5:03 PM
New additions in fellowships, industry, and academic jobs! If you've recently gotten a fellowship or a job (research, industry, PUI) please consider contributing your application materials. It's a helpful resource for those on the job market! github.com/RILAB/statem...
Reposted by Matthew McCullen
mRNAs encoding adjuvant proteins boost immunity. A fantastic study from Hunter et al shows the value of IL12. Study validates original work on this concept, ref below.
Kudos to the authors on this exciting discovery!
www.science.org/doi/10.1126/...
Kudos to the authors on this exciting discovery!
www.science.org/doi/10.1126/...
An Il12 mRNA-LNP adjuvant enhances mRNA vaccine–induced CD8 T cell responses
IL-12 is dispensable for CD8 T cell responses to mRNA vaccination, but an Il12 mRNA-LNP enhances CD8 T cell memory and protection.
www.science.org
June 7, 2025 at 12:40 PM
mRNAs encoding adjuvant proteins boost immunity. A fantastic study from Hunter et al shows the value of IL12. Study validates original work on this concept, ref below.
Kudos to the authors on this exciting discovery!
www.science.org/doi/10.1126/...
Kudos to the authors on this exciting discovery!
www.science.org/doi/10.1126/...
Reposted by Matthew McCullen
🚨 New #preprint alert!
We're excited to share our latest work:
"Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells"🔗 tinyurl.com/4ve26tbd
We're excited to share our latest work:
"Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells"🔗 tinyurl.com/4ve26tbd
June 2, 2025 at 8:33 PM
🚨 New #preprint alert!
We're excited to share our latest work:
"Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells"🔗 tinyurl.com/4ve26tbd
We're excited to share our latest work:
"Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells"🔗 tinyurl.com/4ve26tbd