Allegretti Lab
LightNews LightNews
matteoall.bsky.social
Allegretti Lab
@matteoall.bsky.social
1.1K followers 920 following 11 posts
Cellular structural biology (cryo-EM/ET, CLEM, biophysics) | nature, society, sport and fatherhood | Group Leader at MRC_LMB | https://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/matteo-allegretti/
Posts Replies Media Videos
Reposted by Allegretti Lab
jamesbriscoe.bsky.social
Very sad news, John Gurdon has died.

A developmental biologist's developmental biologist, Nobel prize winner

His work is the foundation of much of today's dev & stem cell bio.

An inspiration to many, including me. Always asking questions & wanting the answers

www.magd.cam.ac.uk/news/profess...
Professor Sir John Gurdon FRS (1933-2025) | Magdalene College
Magdalene College is deeply saddened to announce the death of Professor Sir John Gurdon FRS, who served as Master of the College from 1995 to 2002.
www.magd.cam.ac.uk
October 7, 2025 at 4:02 PM
Reposted by Allegretti Lab
prelights.bsky.social
Another preprint highlight was posted by Kanishka Parashar!

Read how paclitaxel can target cancer cells by destabilising nuclear integrity in interphase - #preprint from @matteoall.bsky.social & team #preprint

#preLight ⬇️👀
prelights.biologists.com/highlights/p...
Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling - preLights
Paclitaxel destabilizes nuclear integrity in interphase by driving microtubule–vimentin aggregates towards the nucleus, leading to SUN2 dependent loss of Lamin A/C loss and enhanced cancer cell vulner...
prelights.biologists.com
September 18, 2025 at 1:47 PM
Reposted by Allegretti Lab
martinpacesa.bsky.social
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...
August 27, 2025 at 4:14 PM
Reposted by Allegretti Lab
mrclmb.bsky.social
We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches.
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs
August 11, 2025 at 2:43 PM
Reposted by Allegretti Lab
rezamehdipour.bsky.social
We discovered that the SUN5-Nesprin3 LINC complex forms a hexagonal lattice at the sperm HTCA, which radically reshapes the nuclear membrane locally!
Our findings also provide the structural basis for understanding the severe ASS (Acephalic Sperm Syndrome) male infertility phenotype.
August 5, 2025 at 12:41 PM
Reposted by Allegretti Lab
rezamehdipour.bsky.social
This work is the result of a fantastic collaboration with@matteoall.bsky.social!
@warred.bsky.social, @a-dsantos.bsky.social , @alisterburt.bsky.social , @tom-hale.bsky.social, @mrclmb.bsky.social,
August 5, 2025 at 12:41 PM
Reposted by Allegretti Lab
rezamehdipour.bsky.social
We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...
August 5, 2025 at 12:41 PM
Reposted by Allegretti Lab
sjorsscheres.bsky.social
Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...
In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells
In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...
www.biorxiv.org
August 5, 2025 at 6:14 AM
matteoall.bsky.social
Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...
Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2674 - Medical Research Council
Location: Cambridge. Vacancy: Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2674. Closing Date: 31/08/2025, 23:55
mrc.tal.net
August 1, 2025 at 11:48 AM
Reposted by Allegretti Lab
lifeonthewedge.bsky.social
Using #cryoET to investigate plant cell-cell communication 🤩🌿
madic.bsky.social Marcel Dickmanns | 🌿❄️🔬 @madic.bsky.social · Jul 26
New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
July 27, 2025 at 9:31 AM
Reposted by Allegretti Lab
tbharat-lab.bsky.social
Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi

Out in @pnas.org

Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social
@mrclmb.bsky.social @hopitalnecker.bsky.social

www.pnas.org/doi/10.1073/...
June 23, 2025 at 10:26 AM
Reposted by Allegretti Lab
deriverylab.bsky.social
🚨 JOB ALERT 🚨
We're hiring a Research Assistant – no PhD needed!
Join us to explore the molecular mechanisms of receptor sorting in endosomes.
Experience in protein expression/purification, cloning & cell culture desired 🧫🔬
Apply now 👉 www.nature.com/naturecareer...

#STEMCareers #ResearchJobs
A picture of 3 rows of people on steps at the MRC LMB. From left to right - top row: Akaash, Vic, Lara - Middle row: Kerrie, Manu, Paddy - bottom row: Meg and Julie
June 23, 2025 at 1:00 PM
Reposted by Allegretti Lab
mrclmb.bsky.social
Congratulations to @tbharat-lab.bsky.social, Group Leader in the LMB’s Structural Studies Division, who has received the Gold Medal from @embo.org for his research on prokaryotic surface molecules.

Read more here: www2.mrc-lmb.cam.ac.uk/tanmay-bhara...

#LMBNews
Portrait photo of Tanmay Bharat
June 5, 2025 at 12:08 PM
Reposted by Allegretti Lab
philholliger.bsky.social
New paper from my group rdcu.be/eoaRr "Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme" in Nature Chemistry — great collaboration with attwaterlab.uk.
Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme
Nature Chemistry - Models of abiotic RNA replication suffer from inherent product inhibition arising from the high stability of RNA duplexes. Now, it has been shown that RNA trinucleotide...
rdcu.be
May 29, 2025 at 3:55 PM
Reposted by Allegretti Lab
cellarchlab.com
“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬
phaips.vd.st Philippe Van der Stappen @phaips.vd.st · May 6
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!

tomoguide.github.io

Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 7, 2025 at 10:56 AM
Reposted by Allegretti Lab
gaiapigino.bsky.social
“Join the Pigino Lab! We’re hiring a PhD student to explore primary cilia biology using advanced imaging, spatial ‘omics, and AI. Apply now at www.cilia-ai.eu and be part of the Cilia-AI Doctoral Network!”
florianjug.bsky.social Florian Jug @florianjug.bsky.social · Jan 14
👀 Looking for a PhD position but you are torn between cilia biology and computational AI research? 😜
The Cilia-AI Doctoral Network has you covered! Lots of fantastic projects throughout Europe, also @humantechnopole.bsky.social with us or @gaiapigino.bsky.social - apply now! ‼️
www.cilia-ai.eu
February 19, 2025 at 8:41 PM
Reposted by Allegretti Lab
tbharat-lab.bsky.social
Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.
plosbiology.org PLOS Biology @plosbiology.org · Apr 22
The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw
Left: The atomic model of the PS2 lattice is shown in top and side views. PS2 hexamers are repeated in the 2D sheet with a lattice constant of 176 Å. Each PS2 monomer is colored and labeled separately within the hexamer, which repeats throughout the lattice (with pore dimensions highlighted). The side view of the lattice shows the smooth surface facing the extracellular space and rough surface with coiled-coil segments protruding towards the MM. Top right: Interactions at the hexameric and trimeric interfaces are stabilized by both hydrophilic and hydrophobic residues. Bottom right: A cell envelope tomogram was used to overlay the cryo-EM structure of the S-layer onto the in situ S-layer tomographic density. A single slice of the tomogram is shown, with the overlayed PS2 S-layer lattice in green and the tomogram in grayscale. The S-layer (surface layer), MM (mycomembrane), IM (inner membrane), and Cytosol are labeled.
April 23, 2025 at 2:07 PM
Reposted by Allegretti Lab
suyangzhang.bsky.social
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.

www.biorxiv.org/content/10.1...

#cryoEM #transcription #splicing
March 24, 2025 at 3:56 PM
Reposted by Allegretti Lab
svenklumpe.bsky.social
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
March 5, 2025 at 4:02 PM
Reposted by Allegretti Lab
gwgl.bsky.social
science.org/doi/10.1126/... Just published! We uncovered a molecular machine that connects the cell's two major structural networks revealing how cells accomplish precise internal reorganization - a process disrupted in diseases ranging from cancer to multiple sclerosis.
Active microtubule-actin cross-talk mediated by a nesprin-2G–kinesin complex
Nesprin-2 Giant links the nucleus to the cytoskeleton, enabling actin transport and nuclear movement through kinesin-1 activation.
science.org
February 21, 2025 at 8:35 PM
Reposted by Allegretti Lab
sjorsscheres.bsky.social
We have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳
Re-posts appreciated.

www.nature.com/naturecareer...
Research Group Leader Tenure Track - Structural Studies - LMB 2549 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12835196
Research Group Leader Tenure Track Starting Salary £65,940 to £74,448 per annum MRC Laboratory of Molecular Biology, Cambridge, UK
www.nature.com
January 27, 2025 at 9:38 AM
matteoall.bsky.social
Ever wondered how the chemotherapeutic drug paclitaxel can target slow-dividing tumours? Check out our preprint Hale et al! @tom-hale.bsky.social found a novel non-mitotic mechanism of action for paclitaxel involving nuclear-cytoskeletal coupling via the LINC complex!
www.biorxiv.org/content/10.1...
January 20, 2025 at 2:47 PM
Reposted by Allegretti Lab
landerlab.bsky.social
Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...
January 16, 2025 at 10:57 PM
Reposted by Allegretti Lab
cellarchlab.com
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇
January 6, 2025 at 11:19 AM
Reposted by Allegretti Lab
cellbiol-mrclmb.bsky.social
Wonderful to see that our colleague @marta-shahbazi.bsky.social is now an EMBO YIP. www.embo.org/press-releas...
Twenty-seven scientists become EMBO Young Investigators – Press releases – EMBO
The group leaders join an international network of nearly 800 life scientists and receive financial support
www.embo.org
December 3, 2024 at 4:36 PM