Manuel Kleiner
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manuelkleiner.bsky.social
Manuel Kleiner
@manuelkleiner.bsky.social
Associate Professor, Microbiomes and Complex Microbial Communities, North Carolina State University
Microbiology, Host-Microbe Interactions, Microbial Ecophysiology, Proteomics and Metaproteomics
https://kleinerlab.cals.ncsu.edu/
If you are interested in #metaproteomics think about joining the 7th International Metaproteomics Symposium next June in Germany www.hs-anhalt.de/landingsites...
I have been at several and they were always awesome
Welcome
www.hs-anhalt.de
November 11, 2025 at 6:24 PM
Reposted by Manuel Kleiner
Our latest Research Briefing is out, on using metaproteomics and stable isotope labelling to uncover rare species involved in carbon turnover www.nature.com/articles/s41...
Identifying rare and active species that drive carbon turnover in complex microbiotas - Nature Microbiology
By combining bioorthogonal non-canonical amino acid tagging, metaproteomics and stable isotope probing, we identified a rare and uncharacterized bacterium with a glycine-mediated metabolism for syntro...
www.nature.com
October 23, 2025 at 1:02 PM
Reposted by Manuel Kleiner
Here's the link to the system, try it! qedscience.com
@qedscience.bsky.social
q.e.d Science
Critical Thinking AI for constructive criticism and science evaluation
qedscience.com
October 15, 2025 at 12:22 PM
Reposted by Manuel Kleiner
This is gaining traction in anti-COVID vax circles but none of them have clocked that it comes from a dataset spanning 2000-2020. The shots are so powerful they transcend time.
September 30, 2025 at 7:07 AM
Reposted by Manuel Kleiner
Congratulations to #YuzeLi and all co-authors with the publication of rhizoSMASH: a new tool to identify catabolic gene clusters involved in the metabolism of plant root exudates, thus driving rhizosphere colonization. 1/4

rdcu.be/eH8tr
Predicting rhizosphere-competence-related catabolic gene clusters in plant-associated bacteria with rhizoSMASH
Nature Communications - Rhizosphere microbiomes are shaped by root exudation of diverse organic compounds. Here, the rhizoSMASH algorithm is introduced, which maps microbial genes involved in their...
rdcu.be
September 25, 2025 at 12:07 PM
Plant root exudates are important in biotic and abiotic interactions that are critical for plant health. the focus so far is mostly on small molecules. Proteins are an understudied, abundant component of root exudates. Time to explore! www.sciencedirect.com/science/arti...
Root-secreted proteins: an underexplored component of root exudates
Proteins are integral components of root exudates that mediate plant–microbe interactions, nutrient mobilization, and stress responses. Despite their …
www.sciencedirect.com
August 28, 2025 at 1:13 PM
Reposted by Manuel Kleiner
Attention plant proteomics specialists: We are looking for a Head of our new Proteomics Unit at our international and dynamic Max Planck Institute of Molecular Plant Physiology. @mpi-mp-potsdam.bsky.social Applications are welcome until 30. Sept 2025: jobs.mpimp-golm.mpg.de/jobposting/8...
Head of Service Unit Proteomics
jobs.mpimp-golm.mpg.de
August 23, 2025 at 8:27 AM
I am really excited to see this article on basic experimental design principles published. Definitely will be mandatory reading for incoming graduate students in my lab. Maggie Wagner who led this article did an amazing job with the illustrations of basic principles www.nature.com/articles/s41...
How thoughtful experimental design can empower biologists in the omics era - Nature Communications
Here, the authors discuss principles of experimental design that are relevant for all biology research, along with special considerations for projects using -omics approaches, highlighting common expe...
www.nature.com
August 7, 2025 at 7:43 PM
Congratulations to Ayesha Awan in my lab to her first, first author paper from her PhD getting published.
Publishing it as a pre-print really helped as it took a whole year from first submission to publication
pubs.rsc.org/en/Content/A...
July 29, 2025 at 6:53 PM
Reposted by Manuel Kleiner
Bluesky CEO Jay Graber emphasized the importance of critical thinking and developing a generalist skillset for the AI era.
Bluesky's CEO warns you shouldn't 'fully outsource your thinking' to AI
Bluesky CEO Jay Graber emphasized the importance of critical thinking and developing a generalist skillset for the AI era.
www.businessinsider.com
July 29, 2025 at 9:32 AM
Reposted by Manuel Kleiner
Counting soil microbial communities: the impact of qPCR platform and mastermix on accuracy and precision

#FEMS Mcirobiol Ecol by @aoifeduff.bsky.social et al

academic.oup.com/femsec/artic...
Counting soil microbial communities: the impact of qPCR platform and mastermix on accuracy and precision
qPCR is used to measure soil microorganisms but can be affected by inhibitors, change in mastermix, and platform. This study measured precision and accurac
academic.oup.com
July 28, 2025 at 2:08 PM
Reposted by Manuel Kleiner
July issue of @cp-trendsmicrobiol.bsky.social is now online, with our review by @xinmingxu.bsky.social @neftalyl.bsky.social on Composing a microbial symphony and a matching cover illustration by @lizahaart.bsky.social

#SynCom #PlantMicrobiome #MicrobiomeEcology at #LeidenBiology
July 1, 2025 at 2:53 PM
Reposted by Manuel Kleiner
Two Postdoc Opportunities in Environmental Microbiology!

Join us and work on microbial key players exposed to oxygen depletion.

Please share widely!
Deadline: 25 July

More information can be found here:
tinyurl.com/4kwzzt6p
Two postdoc positions on the ecology and physiology of microbial key players exposed to oxygen depletion
Application deadline: 25 July 2025 at 23:59 hours local Danish time
tinyurl.com
June 16, 2025 at 8:02 PM
In this work driven by PhD student Anna Garrell in my lab we show key bacterial functions upregulated during colonization and persistence in in plants by 7 bacterial species using #metaproteomics and subsequent experimental validation of the generated hypotheses
www.biorxiv.org/content/10.1...
Differential metaproteomics of bacteria grown in vitro and in planta reveals functions used during growth on maize roots
Microbes are ubiquitous in the rhizosphere and play crucial roles in plant health, yet the metabolisms and physiologies of individual species in planta remain poorly understood. In this study, we exam...
www.biorxiv.org
June 3, 2025 at 3:10 PM
Reposted by Manuel Kleiner
I'm working on a quickstart guide for people who want to plant native plants, but don't know all the things to consider when choosing plants.

I think this is inherently challenging due to the absolute mountain of caveats that goes into doing this properly.

However.

Here's where we're at.
June 3, 2025 at 1:07 PM
Reposted by Manuel Kleiner
One of the most misleading specifications I've seen from a vendor this year.

The Excedion Pro is able to achieve the 70 Hz (2x faster than the 480) under defined conditions. Specifically, a brand new scan option to run at 3700 res at 200 m/z.

For those used to ppm, that's a 276 ppm mass error.
June 1, 2025 at 2:54 PM
Reposted by Manuel Kleiner
Great review from @garciakevin.bsky.social‬ -> Mutualistic Relationships Between Roots and Soil Microbes Facilitate Plant Potassium Acquisition
Mutualistic Relationships Between Roots and Soil Microbes Facilitate Plant Potassium Acquisition
Soil supports diverse life forms and ecosystems through complex interactions among its components, many of which remain poorly understood. Potassium (K) is a vital macronutrient that plays an essential role in plant growth, enzymatic activities, and stress tolerance, but its bioavailability in soils is limited due to complexation in minerals, such as muscovite, biotite, and feldspars. Therefore, only a small fraction is in exchangeable, plant-accessible form. While K fertilizers are commonly used in agriculture and agroforestry to address K deficiencies, the contributions of soil microbial communities to K solubilization and plant acquisition are increasingly recognized. Soil microbes, including K-solubilizing fungi and bacteria, enhance nutrient availability by decomposing soil components, facilitating K release and plant uptake. Despite early recognition of microbial K solubilization in the late nineteenth century, the role of microbes in supporting plant K acquisition remains underexplored, particularly in natural and agro-ecosystems. This chapter reviews current knowledge on plant K uptake mediated by soil microbes, including endophytic and K-solubilizing fungi, bacteria, and mycorrhizal fungi, to highlight their potential for improving soil K cycling, plant K-use-efficiency, and sustainable agricultural practices.
link.springer.com
May 27, 2025 at 7:40 PM
Reposted by Manuel Kleiner
Love today’s #xkcd comic on
#MassSpec
🤣
May 27, 2025 at 5:54 AM
Reposted by Manuel Kleiner
Exciting news: We have released #FragPipe 23, and it's one of our biggest updates ever. Windows installer, support for TMT on Astral and timsTOF, TMT35, PTM site reports for DIA, improved Astral data handling in #MSFragger, improved diaTracer for diaPASEF data, better Skyline integration, and more!
May 5, 2025 at 4:06 AM
Reposted by Manuel Kleiner
Hey y'all! My department is conducting a search for an assistant or associate professor in bacteriology. It's pretty broadly defined. If you know anyone who's looking for a great department that does awesome science - please send them our way! You can DM me with any questions you have 😀
May 9, 2025 at 7:22 PM
Reposted by Manuel Kleiner
Reposted by Manuel Kleiner
My latest @newscientist.com cartoon
April 28, 2025 at 10:09 AM
Reposted by Manuel Kleiner
This is important work for the exciting next stage of metagenomics #MicroSky
April 28, 2025 at 11:04 AM
Reposted by Manuel Kleiner
It's Thursday! #MattersMicrobial episode 87 is up. This time, Dr. Manuel Kleiner joins the #QualityQuorum to chat about how dietary protein interacts with the microbiome. Please share the #GoodMicrobialWord? @microbetv.bsky.social @asm.org @manuelkleiner.bsky.social

youtu.be/a4DmJ3Qvlnc?...
April 18, 2025 at 12:53 AM