Manuel Kleiner
@manuelkleiner.bsky.social
Associate Professor, Microbiomes and Complex Microbial Communities, North Carolina State University
Microbiology, Host-Microbe Interactions, Microbial Ecophysiology, Proteomics and Metaproteomics
https://kleinerlab.cals.ncsu.edu/
Microbiology, Host-Microbe Interactions, Microbial Ecophysiology, Proteomics and Metaproteomics
https://kleinerlab.cals.ncsu.edu/
Reposted by Manuel Kleiner
As I browse around, I keep an eye out for (what I think) are surprising facts.
This 2021 paper estimated that the USA, China and the UK spend USD 2.5 billion on peer review, based on the contribution made by researchers.
🧵
This 2021 paper estimated that the USA, China and the UK spend USD 2.5 billion on peer review, based on the contribution made by researchers.
🧵
January 6, 2026 at 4:09 PM
As I browse around, I keep an eye out for (what I think) are surprising facts.
This 2021 paper estimated that the USA, China and the UK spend USD 2.5 billion on peer review, based on the contribution made by researchers.
🧵
This 2021 paper estimated that the USA, China and the UK spend USD 2.5 billion on peer review, based on the contribution made by researchers.
🧵
Reposted by Manuel Kleiner
If you work on Dark Oxygen --> come to #AbSciCon2026 this year. 17-22 May, Madison
Join our session on ""Dark Oxygen" Production: where does it happen, how does it work, and what does it mean for astrobiology?"
Submit your abstract until January 14
More info here: agu.confex.com/agu/abscicon...
Join our session on ""Dark Oxygen" Production: where does it happen, how does it work, and what does it mean for astrobiology?"
Submit your abstract until January 14
More info here: agu.confex.com/agu/abscicon...
2026 Astrobiology Science Conference
Molecular oxygen (O₂) is often regarded as a strong biosignature in the search for life. Traditionally the production of O2 has been linked to photosynthesis, yet non-photosynthetic (“dark”) pathways ...
agu.confex.com
January 6, 2026 at 5:14 PM
If you work on Dark Oxygen --> come to #AbSciCon2026 this year. 17-22 May, Madison
Join our session on ""Dark Oxygen" Production: where does it happen, how does it work, and what does it mean for astrobiology?"
Submit your abstract until January 14
More info here: agu.confex.com/agu/abscicon...
Join our session on ""Dark Oxygen" Production: where does it happen, how does it work, and what does it mean for astrobiology?"
Submit your abstract until January 14
More info here: agu.confex.com/agu/abscicon...
Reposted by Manuel Kleiner
This column in a word: boss-up, y’all. It is not a warm and fuzzy hug, or an outrage sing-along. It’s a clear reflection of the present, a wake up call, and a preview of the future. We aren’t going to complain Trump away. It’s survival time. www.nature.com/articles/d41...
Dear US researchers: break the outrage addiction. I survived the besieging of science. So can you
As I watch US researchers respond to threats against science, I’m reminded of when scientists in Brazil navigated a similar storm.
www.nature.com
January 4, 2026 at 5:03 PM
This column in a word: boss-up, y’all. It is not a warm and fuzzy hug, or an outrage sing-along. It’s a clear reflection of the present, a wake up call, and a preview of the future. We aren’t going to complain Trump away. It’s survival time. www.nature.com/articles/d41...
Reposted by Manuel Kleiner
Troubleshooting common errors in assemblies of long-read metagenomes - @merenbey.bsky.social @banfieldlab.bsky.social go.nature.com/44P7nSm
Troubleshooting common errors in assemblies of long-read metagenomes - Nature Biotechnology
Long-read sequence assemblies from metagenomes contain frequent errors.
go.nature.com
January 2, 2026 at 4:39 PM
Troubleshooting common errors in assemblies of long-read metagenomes - @merenbey.bsky.social @banfieldlab.bsky.social go.nature.com/44P7nSm
December 30, 2025 at 6:20 PM
Interested in learning how #metaproteomics workflows work and what you can use them for. I am part of a team giving a three day workshop on metaproteomics at Ben Gurion University in late January 2026. Details and registration here lifewp.bgu.ac.il/wp/psme/
Home
lifewp.bgu.ac.il
December 29, 2025 at 8:55 PM
Interested in learning how #metaproteomics workflows work and what you can use them for. I am part of a team giving a three day workshop on metaproteomics at Ben Gurion University in late January 2026. Details and registration here lifewp.bgu.ac.il/wp/psme/
Reposted by Manuel Kleiner
Bacteriophages are normally classified as either virulent or temperate. Reality is, of course, more complicated!
Here we show many bacterial isolates contain non-temperate phages that can persist through restreaking.
Thanks to all co-authors for such a great collaboration!
Here we show many bacterial isolates contain non-temperate phages that can persist through restreaking.
Thanks to all co-authors for such a great collaboration!
[1/3] Is your favorite bacterial isolate hiding a surprise lytic virus with a VERY different lifestyle? Finally I can proudly say that our paper on persistent phages is out in Nature Microbiology. rdcu.be/eWJEp. Well done @peterdoug.bsky.social
Persistent virulent phages exist across bacterial isolates
Nature Microbiology - The long-term existence of diverse virulent phages within cultures of Escherichia coli and others challenges the virulent–temperate dichotomy and points to non-canonical...
rdcu.be
December 29, 2025 at 3:18 PM
Bacteriophages are normally classified as either virulent or temperate. Reality is, of course, more complicated!
Here we show many bacterial isolates contain non-temperate phages that can persist through restreaking.
Thanks to all co-authors for such a great collaboration!
Here we show many bacterial isolates contain non-temperate phages that can persist through restreaking.
Thanks to all co-authors for such a great collaboration!
Reposted by Manuel Kleiner
I’m old enough to remember when CBS News would never have surrendered to a demagogic president or any other politician. Remember Edward R. Murrow?
December 22, 2025 at 6:01 PM
I’m old enough to remember when CBS News would never have surrendered to a demagogic president or any other politician. Remember Edward R. Murrow?
We are recruiting a Lab Manager/Research Specialist
jobs.ncsu.edu/postings/225...
Please re-post, thank you
jobs.ncsu.edu/postings/225...
Please re-post, thank you
Research and Lab Specialist
The Kleiner Lab at North Carolina State University (www.kleinerlab.org) is seeking a Research Specialist to support ongoing research on complex microbial communities, microbial symbioses, and metaprot...
jobs.ncsu.edu
December 12, 2025 at 7:12 PM
We are recruiting a Lab Manager/Research Specialist
jobs.ncsu.edu/postings/225...
Please re-post, thank you
jobs.ncsu.edu/postings/225...
Please re-post, thank you
Super excited to have this paper out. We developed a complex set of ground truth samples to test more than 110 statistical approaches for differential #metaproteomics
We can now give clear guidance on what tests perform well. Thank you Tjorven Hinzke and
@benoitkunath.bsky.social for the leadership
We can now give clear guidance on what tests perform well. Thank you Tjorven Hinzke and
@benoitkunath.bsky.social for the leadership
Evaluation of statistical approaches for differential metaproteomics
Metaproteomics characterizes and compares molecular phenotypes of organisms in communities by comprehensively analyzing their protein expression profiles using statistical methods. However, not all st...
www.biorxiv.org
December 11, 2025 at 8:39 PM
Super excited to have this paper out. We developed a complex set of ground truth samples to test more than 110 statistical approaches for differential #metaproteomics
We can now give clear guidance on what tests perform well. Thank you Tjorven Hinzke and
@benoitkunath.bsky.social for the leadership
We can now give clear guidance on what tests perform well. Thank you Tjorven Hinzke and
@benoitkunath.bsky.social for the leadership
Reposted by Manuel Kleiner
The direct causal evidence of ultra-processed foods for adverse health outcomes is accruing
nature.com/articles/s41...
nature.com/articles/s41...
December 5, 2025 at 9:34 PM
The direct causal evidence of ultra-processed foods for adverse health outcomes is accruing
nature.com/articles/s41...
nature.com/articles/s41...
Reposted by Manuel Kleiner
The most precious commodity you have is your attention. You don’t have to waste it on poor-faith debates or arguments with strangers if you don’t think they’ll be productive. You can prioritize the things that matter to you and make your life richer.
November 30, 2025 at 8:00 PM
The most precious commodity you have is your attention. You don’t have to waste it on poor-faith debates or arguments with strangers if you don’t think they’ll be productive. You can prioritize the things that matter to you and make your life richer.
In fermented foods microbes convert some of the food substrate into microbial protein. We found that in some fermented foods up to 10% of the total protein is microbial and that some of these microbial proteins have functions that might explain some of the beneficial effects of fermented foods.
Assessing the diversity and functional profile of the “microbial proteome” in fermented foods
Fermented foods are staples in diets worldwide and are known for their health benefits. Microorganisms are the key to fermented food production as they convert raw substrates into digestible, nutritio...
www.biorxiv.org
November 23, 2025 at 8:09 PM
In fermented foods microbes convert some of the food substrate into microbial protein. We found that in some fermented foods up to 10% of the total protein is microbial and that some of these microbial proteins have functions that might explain some of the beneficial effects of fermented foods.
Reposted by Manuel Kleiner
"essays produced by ChatGPT are soulless, boring abominations. Words, phrases and punctuation rarely used by the average college student are pervasive." www.huffpost.com/entry/histor...
I Set A Trap To Catch My Students Cheating With AI. The Results Were Shocking.
"Students are not just undermining their ability to learn, but to someday lead."
www.huffpost.com
November 21, 2025 at 12:39 PM
"essays produced by ChatGPT are soulless, boring abominations. Words, phrases and punctuation rarely used by the average college student are pervasive." www.huffpost.com/entry/histor...
Reposted by Manuel Kleiner
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
doi.org
July 2, 2025 at 10:07 AM
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Reposted by Manuel Kleiner
Diving through the purple sulfur bacteria layer of Fayetteville Green Lake with our ROV last month. This is the most intense density of PSB that I've seen in many years!
November 8, 2025 at 6:30 PM
Diving through the purple sulfur bacteria layer of Fayetteville Green Lake with our ROV last month. This is the most intense density of PSB that I've seen in many years!
If you are interested in #metaproteomics think about joining the 7th International Metaproteomics Symposium next June in Germany www.hs-anhalt.de/landingsites...
I have been at several and they were always awesome
I have been at several and they were always awesome
Welcome
www.hs-anhalt.de
November 11, 2025 at 6:24 PM
If you are interested in #metaproteomics think about joining the 7th International Metaproteomics Symposium next June in Germany www.hs-anhalt.de/landingsites...
I have been at several and they were always awesome
I have been at several and they were always awesome
Reposted by Manuel Kleiner
Our latest Research Briefing is out, on using metaproteomics and stable isotope labelling to uncover rare species involved in carbon turnover www.nature.com/articles/s41...
Identifying rare and active species that drive carbon turnover in complex microbiotas - Nature Microbiology
By combining bioorthogonal non-canonical amino acid tagging, metaproteomics and stable isotope probing, we identified a rare and uncharacterized bacterium with a glycine-mediated metabolism for syntro...
www.nature.com
October 23, 2025 at 1:02 PM
Our latest Research Briefing is out, on using metaproteomics and stable isotope labelling to uncover rare species involved in carbon turnover www.nature.com/articles/s41...
Reposted by Manuel Kleiner
q.e.d Science
Critical Thinking AI for constructive criticism and science evaluation
qedscience.com
October 15, 2025 at 12:22 PM
Reposted by Manuel Kleiner
This is gaining traction in anti-COVID vax circles but none of them have clocked that it comes from a dataset spanning 2000-2020. The shots are so powerful they transcend time.
September 30, 2025 at 7:07 AM
This is gaining traction in anti-COVID vax circles but none of them have clocked that it comes from a dataset spanning 2000-2020. The shots are so powerful they transcend time.
Reposted by Manuel Kleiner
Congratulations to #YuzeLi and all co-authors with the publication of rhizoSMASH: a new tool to identify catabolic gene clusters involved in the metabolism of plant root exudates, thus driving rhizosphere colonization. 1/4
rdcu.be/eH8tr
rdcu.be/eH8tr
Predicting rhizosphere-competence-related catabolic gene clusters in plant-associated bacteria with rhizoSMASH
Nature Communications - Rhizosphere microbiomes are shaped by root exudation of diverse organic compounds. Here, the rhizoSMASH algorithm is introduced, which maps microbial genes involved in their...
rdcu.be
September 25, 2025 at 12:07 PM
Congratulations to #YuzeLi and all co-authors with the publication of rhizoSMASH: a new tool to identify catabolic gene clusters involved in the metabolism of plant root exudates, thus driving rhizosphere colonization. 1/4
rdcu.be/eH8tr
rdcu.be/eH8tr
Plant root exudates are important in biotic and abiotic interactions that are critical for plant health. the focus so far is mostly on small molecules. Proteins are an understudied, abundant component of root exudates. Time to explore! www.sciencedirect.com/science/arti...
Root-secreted proteins: an underexplored component of root exudates
Proteins are integral components of root exudates that mediate plant–microbe interactions, nutrient mobilization, and stress responses. Despite their …
www.sciencedirect.com
August 28, 2025 at 1:13 PM
Plant root exudates are important in biotic and abiotic interactions that are critical for plant health. the focus so far is mostly on small molecules. Proteins are an understudied, abundant component of root exudates. Time to explore! www.sciencedirect.com/science/arti...
Reposted by Manuel Kleiner
Attention plant proteomics specialists: We are looking for a Head of our new Proteomics Unit at our international and dynamic Max Planck Institute of Molecular Plant Physiology. @mpi-mp-potsdam.bsky.social Applications are welcome until 30. Sept 2025: jobs.mpimp-golm.mpg.de/jobposting/8...
Head of Service Unit Proteomics
jobs.mpimp-golm.mpg.de
August 23, 2025 at 8:27 AM
Attention plant proteomics specialists: We are looking for a Head of our new Proteomics Unit at our international and dynamic Max Planck Institute of Molecular Plant Physiology. @mpi-mp-potsdam.bsky.social Applications are welcome until 30. Sept 2025: jobs.mpimp-golm.mpg.de/jobposting/8...
I am really excited to see this article on basic experimental design principles published. Definitely will be mandatory reading for incoming graduate students in my lab. Maggie Wagner who led this article did an amazing job with the illustrations of basic principles www.nature.com/articles/s41...
How thoughtful experimental design can empower biologists in the omics era - Nature Communications
Here, the authors discuss principles of experimental design that are relevant for all biology research, along with special considerations for projects using -omics approaches, highlighting common expe...
www.nature.com
August 7, 2025 at 7:43 PM
I am really excited to see this article on basic experimental design principles published. Definitely will be mandatory reading for incoming graduate students in my lab. Maggie Wagner who led this article did an amazing job with the illustrations of basic principles www.nature.com/articles/s41...
Congratulations to Ayesha Awan in my lab to her first, first author paper from her PhD getting published.
Publishing it as a pre-print really helped as it took a whole year from first submission to publication
pubs.rsc.org/en/Content/A...
Publishing it as a pre-print really helped as it took a whole year from first submission to publication
pubs.rsc.org/en/Content/A...
July 29, 2025 at 6:53 PM
Congratulations to Ayesha Awan in my lab to her first, first author paper from her PhD getting published.
Publishing it as a pre-print really helped as it took a whole year from first submission to publication
pubs.rsc.org/en/Content/A...
Publishing it as a pre-print really helped as it took a whole year from first submission to publication
pubs.rsc.org/en/Content/A...