Filippov ADPr lab
@leidenadpr.bsky.social
Bio-organic synthetic lab with focus on the synthesis of ADP-ribose related biopolymers.
Reposted by Filippov ADPr lab
Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...
Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology
RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...
www.nature.com
July 9, 2025 at 12:00 PM
Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...
Reposted by Filippov ADPr lab
Excited to share my PhD work in the Kranzusch Lab published in @nature.com!
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
A DNA-gated molecular guard controls bacterial Hailong anti-phage defence - Nature
Nature - A DNA-gated molecular guard controls bacterial Hailong anti-phage defence
www.nature.com
April 30, 2025 at 6:57 PM
Excited to share my PhD work in the Kranzusch Lab published in @nature.com!
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
Two key discoveries:
- Nucleotides can act as negative regulators of antiviral immunity
- Ion channel activation is gated by DNA
Thank you to our all collaborators! @soreklab.bsky.social
www.nature.com/articles/s41...
Reposted by Filippov ADPr lab
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
April 30, 2025 at 6:44 PM
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Reposted by Filippov ADPr lab
We have a new article out in collaboration with the group of Michiel Vermeulen at @nkinl.bsky.social
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
February 11, 2025 at 9:46 AM
We have a new article out in collaboration with the group of Michiel Vermeulen at @nkinl.bsky.social
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Filippov ADPr lab
Interesting paper by the groups of Dima Filippov @leidenadpr.bsky.social @led3hub.bsky.social and @michielvermeulen.bsky.social in ChemBioChem. They developed a biotinylated photoaffinity probe of the PARP inhibitor Olaparib and used it to profile off-targets. chemistry-europe.onl...
March 19, 2025 at 7:00 AM
Interesting paper by the groups of Dima Filippov @leidenadpr.bsky.social @led3hub.bsky.social and @michielvermeulen.bsky.social in ChemBioChem. They developed a biotinylated photoaffinity probe of the PARP inhibitor Olaparib and used it to profile off-targets. chemistry-europe.onl...
Reposted by Filippov ADPr lab
Celebrating 14 years of science, teamwork, and resilience! To mark our lab’s anniversary, we traded pipettes for pottery—turns out shaping clay is just as therapeutic as shaping experiments. With NIH funding uncertainties, a little calm goes a long way. Grateful for this team! #LabLife
February 22, 2025 at 12:29 AM
Celebrating 14 years of science, teamwork, and resilience! To mark our lab’s anniversary, we traded pipettes for pottery—turns out shaping clay is just as therapeutic as shaping experiments. With NIH funding uncertainties, a little calm goes a long way. Grateful for this team! #LabLife
Reposted by Filippov ADPr lab
Check out our approach toward disciformycin and gulmirecin, spearheaded by Peter Ruehmann @nyuchemistry.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
February 15, 2025 at 12:47 PM
Check out our approach toward disciformycin and gulmirecin, spearheaded by Peter Ruehmann @nyuchemistry.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by Filippov ADPr lab
💯 👇
Many in biotech/private sector are boasting about their ways of funding and doing science
But they are staffed with PhDs and postdocs we train in our NIH and NSF funded labs in universities
With university labs lacking funds to train, the private sector will be profoundly harmed
But they are staffed with PhDs and postdocs we train in our NIH and NSF funded labs in universities
With university labs lacking funds to train, the private sector will be profoundly harmed
February 12, 2025 at 2:44 AM
💯 👇
Reposted by Filippov ADPr lab
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 12, 2025 at 6:28 AM
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Filippov ADPr lab
Excited to share our new review on biomolecular condensates mediated by PARPs and ADP-ribosylation @ Trends in Biochemical Sciences!
Despite the uncertainty, science must march forward. Grateful for the support that makes discovery possible. #FundScience #KeepScienceStrong
Despite the uncertainty, science must march forward. Grateful for the support that makes discovery possible. #FundScience #KeepScienceStrong
Available online - the Review "PARPs and ADP-ribosylation-mediated #biomolecularcondensates: determinants, dynamics, and disease implications" from @leunglab.bsky.social and colleagues.
#phaseseparation #proteincondensation #proteinaggregation
Free access at: authors.elsevier.com/a/1kZlO3S6Gf...
#phaseseparation #proteincondensation #proteinaggregation
Free access at: authors.elsevier.com/a/1kZlO3S6Gf...
February 12, 2025 at 5:11 AM
Excited to share our new review on biomolecular condensates mediated by PARPs and ADP-ribosylation @ Trends in Biochemical Sciences!
Despite the uncertainty, science must march forward. Grateful for the support that makes discovery possible. #FundScience #KeepScienceStrong
Despite the uncertainty, science must march forward. Grateful for the support that makes discovery possible. #FundScience #KeepScienceStrong
We have a new article out in collaboration with the group of Michiel Vermeulen at @nkinl.bsky.social
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
February 11, 2025 at 9:46 AM
We have a new article out in collaboration with the group of Michiel Vermeulen at @nkinl.bsky.social
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Photoaffinity probes based on olaparib for Proteomics profiling. Simple but powerful 💪
Read here: chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Filippov ADPr lab
Nice work from the Andexer laboratory on PPK2 enzymes and their use in nucleotide synthesis
doi.org/10.1002/cbic...
doi.org/10.1002/cbic...
Structural Insights into Broad‐Range Polyphosphate Kinase 2‐II Enzymes Applicable for Pyrimidine Nucleoside Diphosphate Synthesis
This study reports the first substrate-bound structures of polyphosphate kinase 2-II (PPK2-II) enzymes, offering insights into nucleoside diphosphate (NDP) synthesis. BcPPK2 (Bacillus cereus) and LfP....
doi.org
February 7, 2025 at 8:01 AM
Nice work from the Andexer laboratory on PPK2 enzymes and their use in nucleotide synthesis
doi.org/10.1002/cbic...
doi.org/10.1002/cbic...
Reposted by Filippov ADPr lab
great new insights from Ji Luo, Barry O´Keefe and Brice Wilson on ITPK1 inhibitors
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Biochemical and biophysical characterization of inositol-tetrakisphosphate 1-kinase inhibitors
Inositol phosphates (IPs) and inositol pyrophosphate play critical roles in many biological processes as signaling molecules in pathways responsible f…
www.sciencedirect.com
February 7, 2025 at 8:03 AM
great new insights from Ji Luo, Barry O´Keefe and Brice Wilson on ITPK1 inhibitors
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Filippov ADPr lab
It is that time of year again... our Christmas tree arriving today! 🎄
(photos of the fully decorated tree to follow!)
(photos of the fully decorated tree to follow!)
December 3, 2024 at 4:25 PM
It is that time of year again... our Christmas tree arriving today! 🎄
(photos of the fully decorated tree to follow!)
(photos of the fully decorated tree to follow!)
Reposted by Filippov ADPr lab
Our Synthesis Tag (SynTag) for the synthesis of aggregating peptides and proteins is now published in JACS 🥳 Congratulations to first authors Héloïse & Elyse and everyone else involved ✨✨
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
A Versatile “Synthesis Tag” (SynTag) for the Chemical Synthesis of Aggregating Peptides and Proteins
Solid-phase peptide synthesis (SPPS) and native chemical ligation (NCL) are powerful methods for obtaining peptides and proteins that are otherwise inaccessible. Nonetheless, numerous sequences are di...
pubs.acs.org
December 6, 2024 at 6:39 AM
Our Synthesis Tag (SynTag) for the synthesis of aggregating peptides and proteins is now published in JACS 🥳 Congratulations to first authors Héloïse & Elyse and everyone else involved ✨✨
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by Filippov ADPr lab
Interesting paper by the @roelfesgroup.bsky.social and the group of Daniel Sauer in @angewandtechemie.bsky.social. They used genetic code expansion to incorporate a thiophenol-containing amino acid into a protein and used it to make an artificial gold enzyme. onlinelibrary.wiley.com/doi/10.1002/...
Artificial Gold Enzymes Using a Genetically Encoded Thiophenol‐Based Noble‐Metal‐Binding Ligand
Incorporating noble metals in artificial metalloenzymes (ArMs) is challenging due to the lack of suitable soft coordinating ligands among natural amino acids. We present a new class of ArMs featuring...
onlinelibrary.wiley.com
December 6, 2024 at 10:54 AM
Interesting paper by the @roelfesgroup.bsky.social and the group of Daniel Sauer in @angewandtechemie.bsky.social. They used genetic code expansion to incorporate a thiophenol-containing amino acid into a protein and used it to make an artificial gold enzyme. onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Filippov ADPr lab
Interesting workshop organized by the Young Academy Leiden about public outreach including through publishing news articles for the general public e.g. with @uk.theconversation.com. Great insights, how to pitch an idea to a publisher. Thank you Mike Herd for the presentation. www.theconversation.com
December 6, 2024 at 5:07 PM
Interesting workshop organized by the Young Academy Leiden about public outreach including through publishing news articles for the general public e.g. with @uk.theconversation.com. Great insights, how to pitch an idea to a publisher. Thank you Mike Herd for the presentation. www.theconversation.com
Reposted by Filippov ADPr lab
Gut microbiota-derived GlcNAc-MurNAc is a TLR4 agonist that protects the host gut https://www.biorxiv.org/content/10.1101/2024.11.27.625627v1
Gut microbiota-derived GlcNAc-MurNAc is a TLR4 agonist that protects the host gut https://www.biorxiv.org/content/10.1101/2024.11.27.625627v1
Gut microbiota-derived peptidoglycan fragments (PGNs) are key signaling molecules that regulate mult
www.biorxiv.org
November 29, 2024 at 2:47 AM
Gut microbiota-derived GlcNAc-MurNAc is a TLR4 agonist that protects the host gut https://www.biorxiv.org/content/10.1101/2024.11.27.625627v1
Reposted by Filippov ADPr lab
It is great to have @aekiib.bsky.social from the group of @tbpoulsen.bsky.social as visiting PhD student in the lab. His stay in our lab allows us to exchange a lot of thoughts and technologies between the labs and learn so much from each other. #ChemBio onlinelibrary.wiley....
November 26, 2024 at 4:02 PM
It is great to have @aekiib.bsky.social from the group of @tbpoulsen.bsky.social as visiting PhD student in the lab. His stay in our lab allows us to exchange a lot of thoughts and technologies between the labs and learn so much from each other. #ChemBio onlinelibrary.wiley....
Reposted by Filippov ADPr lab
'Skeetorial' of our latest paper: link.springer.com/article/10.1...
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
November 24, 2024 at 12:18 PM
'Skeetorial' of our latest paper: link.springer.com/article/10.1...
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
Reposted by Filippov ADPr lab
Amazing to see, how this platform is growing. This post is only 9 days old and my number of followers has tripled since then to over 1500. Very excited that we have an active scientific community on this platform again. Follow me for updates on the scientific literature with a focus on #ChemBio.
Very excited to see the scientific community forming over here and to have reached 500 followers. I will try to ramp up my activities again posting on literature on #Science, #Chemistry, #Chemsky, #ChemicalBiology, #Antibiotics, #CovalentInhibitors, #ChemicalProteomics and #ABPP.
November 24, 2024 at 7:08 PM
Amazing to see, how this platform is growing. This post is only 9 days old and my number of followers has tripled since then to over 1500. Very excited that we have an active scientific community on this platform again. Follow me for updates on the scientific literature with a focus on #ChemBio.
Reposted by Filippov ADPr lab
Excellent study demonstrating that chronically low NMNAT2 levels in neurons leads to NMN-induced SARM1 activation and subsequent axon degeneration. Their findings provide critical insight into the pathogenesis of partial loss-of-function mutations in NMNAT2 in humans with early-onset neuropathy.
'Skeetorial' of our latest paper: link.springer.com/article/10.1...
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
So proud of first author Christina Antoniou for this, now a postdoc with @melissa-gammons1.bsky.social.
(1) NMNAT2 is a repressor of SARM1 NADase in axons. A few people have NMNAT2 LoF mutations. Many more have low expression.
November 24, 2024 at 4:49 PM
Excellent study demonstrating that chronically low NMNAT2 levels in neurons leads to NMN-induced SARM1 activation and subsequent axon degeneration. Their findings provide critical insight into the pathogenesis of partial loss-of-function mutations in NMNAT2 in humans with early-onset neuropathy.
Reposted by Filippov ADPr lab
This is such a cool and unexpected story.
Finally moved to #Bluesky
and happy to share our recent work about a new phage anti-defense system, based on NAD reconstitution by new enzymatic reactions.
www.nature.com/articles/s41...
and happy to share our recent work about a new phage anti-defense system, based on NAD reconstitution by new enzymatic reactions.
www.nature.com/articles/s41...
November 22, 2024 at 4:15 PM
This is such a cool and unexpected story.
Reposted by Filippov ADPr lab
Amazing talk by Thanja Lamberts at the #ReedijkSymposium. She talked about her work in the field of astrochemistry and the importance of surface chemistry, low temperature, low density, element availability, timescales and telescopes for her research. pubs.acs.org/doi/10.1021/...
November 22, 2024 at 1:26 PM
Amazing talk by Thanja Lamberts at the #ReedijkSymposium. She talked about her work in the field of astrochemistry and the importance of surface chemistry, low temperature, low density, element availability, timescales and telescopes for her research. pubs.acs.org/doi/10.1021/...
Illuminating talk by Zach Armstrong at #ReedijkSymposium in Leiden. Cryo-EM on human fucosidase doi.org/10.1016/j.st... and more fascinating glycoscience
November 22, 2024 at 12:52 PM
Illuminating talk by Zach Armstrong at #ReedijkSymposium in Leiden. Cryo-EM on human fucosidase doi.org/10.1016/j.st... and more fascinating glycoscience