@labnikel.bsky.social
Pinned
Integrated control of redox and energy metabolism by the membrane-bound and soluble transhydrogenases of Pseudomonas putida across metabolic regimes
Redox homeostasis is central to microbial physiology and stress adaptation, yet the functional roles of transhydrogenases remain poorly understood beyond a few organisms. In this study, we systematica...
www.biorxiv.org
How does Pseudomonas putida keep its redox balance steady across different metabolic states?
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Reposted
🤝🌍 Last week, the #TOLERATE EU project consortium meeting took place in Groningen, The Netherlands. Partners came together for two intensive and productive days to share updates, reflect on progress, and align on next steps across all work packages.
December 15, 2025 at 10:19 AM
🤝🌍 Last week, the #TOLERATE EU project consortium meeting took place in Groningen, The Netherlands. Partners came together for two intensive and productive days to share updates, reflect on progress, and align on next steps across all work packages.
Reposted
For all PhD candidates interested in #syntheticbiology consider our #EMBOSynBio course! Applications open!
Apply for #EMBOSynBio! 🥼🧪
The course offers hands-on experience with cutting-edge SynBio tools, supported by lectures covering the whole engineering cycle and its convergence toward a deeper understanding of biology.
30 Jun – 6 Jul 2026
EMBL Heidelberg
s.embl.org/syn26-01-bl
The course offers hands-on experience with cutting-edge SynBio tools, supported by lectures covering the whole engineering cycle and its convergence toward a deeper understanding of biology.
30 Jun – 6 Jul 2026
EMBL Heidelberg
s.embl.org/syn26-01-bl
December 6, 2025 at 7:23 AM
For all PhD candidates interested in #syntheticbiology consider our #EMBOSynBio course! Applications open!
How does Pseudomonas putida keep its redox balance steady across different metabolic states?
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Integrated control of redox and energy metabolism by the membrane-bound and soluble transhydrogenases of Pseudomonas putida across metabolic regimes
Redox homeostasis is central to microbial physiology and stress adaptation, yet the functional roles of transhydrogenases remain poorly understood beyond a few organisms. In this study, we systematica...
www.biorxiv.org
November 9, 2025 at 7:59 PM
How does Pseudomonas putida keep its redox balance steady across different metabolic states?
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Reposted
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin — Led by Leah Bushin, featuring M. Gracia Alvan, @danielvolke.bsky.social @oscarpuiggene.bsky.social in a fantastic collaboration w/Brad Moore @labnikel.bsky.social @natbiotech.nature.com 🦑
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 3, 2025 at 12:49 PM
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin — Led by Leah Bushin, featuring M. Gracia Alvan, @danielvolke.bsky.social @oscarpuiggene.bsky.social in a fantastic collaboration w/Brad Moore @labnikel.bsky.social @natbiotech.nature.com 🦑
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
🔥🔥🔥 Friday Evening, Paper Out, Feierabend 🔥🔥🔥
When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy.
doi.org/10.1016/j.ym...
When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy.
doi.org/10.1016/j.ym...
Redirecting
doi.org
October 24, 2025 at 5:00 PM
🔥🔥🔥 Friday Evening, Paper Out, Feierabend 🔥🔥🔥
When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy.
doi.org/10.1016/j.ym...
When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy.
doi.org/10.1016/j.ym...
🚀 Thrilled to share our new Nature Communications article:
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
A blueprint for designing the next-generation of synthetic C1 microbes - Nature Communications
Synthetic one-carbon assimilation could contribute to a more sustainable and circular carbon economy, but much work in this field has focused on model microorganisms. Here the authors provide their perspective on the potential value of non-model microbes, and how that potential could be realised.
www.nature.com
October 8, 2025 at 9:10 AM
🚀 Thrilled to share our new Nature Communications article:
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
Reposted
From the microbial networks behind kombucha fermentation to engineered strains powering sustainable fuels, #FEMSYeastRes brings you high-quality studies with major relevance across the field. 📊 Read the journal's most-read papers for the first half of 2025 buff.ly/NLKSc4N
August 19, 2025 at 10:02 AM
From the microbial networks behind kombucha fermentation to engineered strains powering sustainable fuels, #FEMSYeastRes brings you high-quality studies with major relevance across the field. 📊 Read the journal's most-read papers for the first half of 2025 buff.ly/NLKSc4N
🚨 New paper in mBio!
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
Synthetic C1 metabolism in Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway | mBio
Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...
journals.asm.org
August 22, 2025 at 10:19 AM
🚨 New paper in mBio!
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
Reposted
#Synthetic C1 #metabolism in #Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway—led by J. Turlin, M. G. Alván, @oscarpuiggene.bsky.social w/@stefanodonati.bsky.social & @sebwenk.bsky.social @mbio.bsky.social #SynBio
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Synthetic C1 metabolism in Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway | mBio
Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...
journals.asm.org
August 21, 2025 at 3:45 PM
#Synthetic C1 #metabolism in #Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway—led by J. Turlin, M. G. Alván, @oscarpuiggene.bsky.social w/@stefanodonati.bsky.social & @sebwenk.bsky.social @mbio.bsky.social #SynBio
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted
🌱💧 Can #bioaugmentation help crops beat drought?
Discover how the #TOLERATEproject is exploring microbial solutions to boost drought resilience in EU agriculture, sustainably and at scale.
👏 Great insights by Afnan Suleiman-Bioclear earth-
🔗 Read more: tolerate-eu-project.com/how-eu-toler...
Discover how the #TOLERATEproject is exploring microbial solutions to boost drought resilience in EU agriculture, sustainably and at scale.
👏 Great insights by Afnan Suleiman-Bioclear earth-
🔗 Read more: tolerate-eu-project.com/how-eu-toler...
How EU TOLERATE Project explores bioaugmentation to combat drought stress in agriculture - Cyprus Institute of Neurology & Genetics
Climate change is intensifying drought events, putting global food production under pressure. Within the EU-funded TOLERATE project, Work Package 3 (WP3), led by Bioclear earth, and in collaboration w...
tolerate-eu-project.com
August 5, 2025 at 8:38 AM
🌱💧 Can #bioaugmentation help crops beat drought?
Discover how the #TOLERATEproject is exploring microbial solutions to boost drought resilience in EU agriculture, sustainably and at scale.
👏 Great insights by Afnan Suleiman-Bioclear earth-
🔗 Read more: tolerate-eu-project.com/how-eu-toler...
Discover how the #TOLERATEproject is exploring microbial solutions to boost drought resilience in EU agriculture, sustainably and at scale.
👏 Great insights by Afnan Suleiman-Bioclear earth-
🔗 Read more: tolerate-eu-project.com/how-eu-toler...
Reposted
After a long sleep, environmental remediation with live biologics is back to the mainstream R&D agenda at this side of the pond. Wanna join and EU-wide on line conversation with bioremediation stakeholders this Nov 10? Express interest and register here 👉 forms.cloud.microsoft/e/b7xVz7dJcu
July 22, 2025 at 10:45 AM
After a long sleep, environmental remediation with live biologics is back to the mainstream R&D agenda at this side of the pond. Wanna join and EU-wide on line conversation with bioremediation stakeholders this Nov 10? Express interest and register here 👉 forms.cloud.microsoft/e/b7xVz7dJcu
Reposted
🌍 At #FEMSMicrobiology 2025, the TOLERATE team had the great pleasure of sharing insights from the TOLERATE project!
🧬 Our session sparked great discussions around ancient DNA and how we can revive, through synthetic biology, lost functions beneficial for future agriculture.
🧬 Our session sparked great discussions around ancient DNA and how we can revive, through synthetic biology, lost functions beneficial for future agriculture.
July 22, 2025 at 11:29 AM
🌍 At #FEMSMicrobiology 2025, the TOLERATE team had the great pleasure of sharing insights from the TOLERATE project!
🧬 Our session sparked great discussions around ancient DNA and how we can revive, through synthetic biology, lost functions beneficial for future agriculture.
🧬 Our session sparked great discussions around ancient DNA and how we can revive, through synthetic biology, lost functions beneficial for future agriculture.
Reposted
📍 Milan | TODAY!
Join Amedea Perfumo & @pabnik.bsky.social at #FEMS 2025 for a dynamic session🧬 "Something (very) old, something new: Innovative microbial solutions for sustainability challenges
🕦 15 July | 11:30–13:00 | Tower Lounge
🔗 femsmicro.org
▶️ youtu.be/0-bhcMpjJ_c
#TolerateEUProject
Join Amedea Perfumo & @pabnik.bsky.social at #FEMS 2025 for a dynamic session🧬 "Something (very) old, something new: Innovative microbial solutions for sustainability challenges
🕦 15 July | 11:30–13:00 | Tower Lounge
🔗 femsmicro.org
▶️ youtu.be/0-bhcMpjJ_c
#TolerateEUProject
July 15, 2025 at 5:51 AM
📍 Milan | TODAY!
Join Amedea Perfumo & @pabnik.bsky.social at #FEMS 2025 for a dynamic session🧬 "Something (very) old, something new: Innovative microbial solutions for sustainability challenges
🕦 15 July | 11:30–13:00 | Tower Lounge
🔗 femsmicro.org
▶️ youtu.be/0-bhcMpjJ_c
#TolerateEUProject
Join Amedea Perfumo & @pabnik.bsky.social at #FEMS 2025 for a dynamic session🧬 "Something (very) old, something new: Innovative microbial solutions for sustainability challenges
🕦 15 July | 11:30–13:00 | Tower Lounge
🔗 femsmicro.org
▶️ youtu.be/0-bhcMpjJ_c
#TolerateEUProject
Preprint finally published on @cp-trendsbiotech.bsky.social! Check out the word led by @oscarpuiggene.bsky.social on methanol assimilation in P. putida via enhanced serine–threonine cycle on www.cell.com/trends/biote...
Below, the original thread🧵scratching the surface of a long yet satisfying work!
Below, the original thread🧵scratching the surface of a long yet satisfying work!
July 13, 2025 at 3:04 PM
Preprint finally published on @cp-trendsbiotech.bsky.social! Check out the word led by @oscarpuiggene.bsky.social on methanol assimilation in P. putida via enhanced serine–threonine cycle on www.cell.com/trends/biote...
Below, the original thread🧵scratching the surface of a long yet satisfying work!
Below, the original thread🧵scratching the surface of a long yet satisfying work!
Reposted
Online now @ Cell is the yeast multicellular engineering paper from Fankang Meng - the fruits of his productive PhD in our group. He developed modular synthetic biology tools to bring multicellular behaviours to yeast - specific adhesion, juxtacrine signalling and more. www.cell.com/cell/fulltex...
Engineering yeast multicellular behaviors via synthetic adhesion and contact signaling
By designing synthetic toolkits for contact-based signaling (MARS) and cell-cell adhesion
(SATURN), we program yeast to form multicellular structures and perform complex tasks,
like building logic cir...
www.cell.com
July 11, 2025 at 3:01 PM
Online now @ Cell is the yeast multicellular engineering paper from Fankang Meng - the fruits of his productive PhD in our group. He developed modular synthetic biology tools to bring multicellular behaviours to yeast - specific adhesion, juxtacrine signalling and more. www.cell.com/cell/fulltex...
The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇
July 9, 2025 at 11:21 AM
The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇
Check out our newest article led by @oscarpuiggene.bsky.social !
Implementing synthetic serine cycles in #Pseudomonas putida unlock methanol assimilation in our favorite bug w/carbon conservation, led by ÒscarPuiggené @labnikel.bsky.social · #SynBio #MetabolicEngineering #Methanol published in @cp-trendsbiotech.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topologies for methanol assimilation
The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic…
www.sciencedirect.com
July 9, 2025 at 11:15 AM
Check out our newest article led by @oscarpuiggene.bsky.social !
Reposted
🧫 @josephinegiard.bsky.social (HWU) presented #TOLERATE at the #MicrobiologySociety Early Career Conference 2025 in Newcastle 🇬🇧. Flash talk + poster led to great discussions on antifungal resistance with >10 peers & >50 attendees! 🌱
#TOLERATE #HorizonEurope #Microbiology
#TOLERATE #HorizonEurope #Microbiology
July 3, 2025 at 1:48 PM
🧫 @josephinegiard.bsky.social (HWU) presented #TOLERATE at the #MicrobiologySociety Early Career Conference 2025 in Newcastle 🇬🇧. Flash talk + poster led to great discussions on antifungal resistance with >10 peers & >50 attendees! 🌱
#TOLERATE #HorizonEurope #Microbiology
#TOLERATE #HorizonEurope #Microbiology
Reposted
New phi15-based expression system in Pseudomonas putida uses #SEVA plasmids for high-yield gene expression. The work by @pabnik.bsky.social shows how single-copy vectors minimise toxicity, achieve 200×induction, and outperform T7 systems. 👉 www.nature.com/articles/s42...
Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida - Communications Biology
The development of a modular, phage-based expression system optimized for Pseudomonas putida enables stringent, tunable and high yield expressions with minimal cytotoxicity.
www.nature.com
July 3, 2025 at 10:02 AM
New phi15-based expression system in Pseudomonas putida uses #SEVA plasmids for high-yield gene expression. The work by @pabnik.bsky.social shows how single-copy vectors minimise toxicity, achieve 200×induction, and outperform T7 systems. 👉 www.nature.com/articles/s42...
Reposted
Today, the #TOLERATEProject held its 1st Review Meeting (M1–M18) with PO & PM highlighting our progress, innovation & first results. A key step to shape the road ahead! 🔬🌱💦
Learn more: https://tolerate-eu-project.com/
#HorizonEurope #EUProjects #Biotechnology #SustainableAgriculture
Learn more: https://tolerate-eu-project.com/
#HorizonEurope #EUProjects #Biotechnology #SustainableAgriculture
June 30, 2025 at 1:02 PM
Today, the #TOLERATEProject held its 1st Review Meeting (M1–M18) with PO & PM highlighting our progress, innovation & first results. A key step to shape the road ahead! 🔬🌱💦
Learn more: https://tolerate-eu-project.com/
#HorizonEurope #EUProjects #Biotechnology #SustainableAgriculture
Learn more: https://tolerate-eu-project.com/
#HorizonEurope #EUProjects #Biotechnology #SustainableAgriculture
Reposted
Ethylene glycol is widely used as monomer of the plastic PET. It is therefore highly relevant as sustainable feedstock for microbial biotechnology. In our new paper in @natcomms.nature.com, we characterize efficient enzymes for ethylene glycol oxidation: www.nature.com/articles/s41...
July 1, 2025 at 2:57 PM
Ethylene glycol is widely used as monomer of the plastic PET. It is therefore highly relevant as sustainable feedstock for microbial biotechnology. In our new paper in @natcomms.nature.com, we characterize efficient enzymes for ethylene glycol oxidation: www.nature.com/articles/s41...
Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
June 23, 2025 at 8:14 AM
Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Reposted
Happy to host @kunjapur.bsky.social as a sabbatical #professor at DTU Biosustain, supported by Otto Mønsteds Fond! We share a passion for using #SynBio to engineer #microbes with non-natural building blocks for advanced #biotechology. He’s in #Europe — drop a line to connect & discuss cool #Science!
June 9, 2025 at 9:00 AM
Happy to host @kunjapur.bsky.social as a sabbatical #professor at DTU Biosustain, supported by Otto Mønsteds Fond! We share a passion for using #SynBio to engineer #microbes with non-natural building blocks for advanced #biotechology. He’s in #Europe — drop a line to connect & discuss cool #Science!
Reposted
🎉 Excited to share that I’ve received an Emerging Investigator Grant from the Novo Nordisk Foundation! I’ll start my research group in Jan 2026 to develop universal cell factories for P2X & CO₂-based biomanufacturing 🌱🔬
👉 researchleaderprogramme.com/recipients/e...
👉 researchleaderprogramme.com/recipients/e...
June 3, 2025 at 1:56 PM
🎉 Excited to share that I’ve received an Emerging Investigator Grant from the Novo Nordisk Foundation! I’ll start my research group in Jan 2026 to develop universal cell factories for P2X & CO₂-based biomanufacturing 🌱🔬
👉 researchleaderprogramme.com/recipients/e...
👉 researchleaderprogramme.com/recipients/e...
Reposted
The TOLERATE team met at DTU (Denmark) for a productive General Assembly.
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
June 2, 2025 at 4:33 PM
The TOLERATE team met at DTU (Denmark) for a productive General Assembly.
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects