4/4 By integrating:
✔️ Life cycle assessment (low-carbon substrates)
✔️ Techno-economic insights (from the start)
…we provide a path toward efficient, sustainable, scalable C1 biomanufacturing.

Great insight by @giusifav.bsky.social and @oscarpuiggene.bsky.social!

3/4 Our perspective lays out:
1️⃣ A blueprint for designing next-gen synthetic C1 microbes
2️⃣ A roadmap for engineering P. putida into an autotrophic chassis
3️⃣ A circular carbon economy vision, revalorizing CO₂ + waste streams
📊 Together, these steps connect design → engineering → sustainability.

2/4 Most work on synthetic C1 assimilation has focused on model microbes. But:
🧬 Non-canonical hosts bring untapped metabolic advantages
⚡ They tolerate diverse substrates & have unique enzymes
🌍 They could make C1-based processes more scalable & sustainable

A discovery journey of almost 4 years initiated by J. Turlin, and then continued by M. G. Alván, @oscarpuiggene.bsky.social with the precious collaboration of @stefanodonati.bsky.social and @sebwenk.bsky.social!
5/5

✅ The combination of these strategies enabled strict formatotrophy and could be applied to establish also methylotrophy in P. putida.
A step toward sustainable bioproduction from C1 substrates! 🌱
4/5

🧩 In the current study, we went further:
– Rational engineering
– Pathway modularization
– Growth-coupled selection
– Adaptive laboratory evolution (ALE)

3/5

🔬 Starting point: P. putida engineered with rGlyP could assimilate formate under mixotrophic conditions—using acetate for energy conservation.

We previously described more in detail this endeavor:
www.sciencedirect.com/science/arti...
2/5

Are you interested in:
• Getting to know about the latest research in the field
• Establishing new collaborations
• Discussing tools/technologies for engineering & analyzing Pseudomonas
• Showing your Peers what you are doing
Then reach out to us (chdavo@dtu.dk).
Participation is free of charge.