Jose Maldonado, PhD
@josemald.bsky.social
Research Assistant Professor, Molecular Physiology and Biophysics, Vanderbilt University
I help labs integrate lightsheet microscopy into their new and ongoing research. I like to play the drums.
VanderbiltNL.com
https://orcid.org/0000-0002-8008-7059
I help labs integrate lightsheet microscopy into their new and ongoing research. I like to play the drums.
VanderbiltNL.com
https://orcid.org/0000-0002-8008-7059
Pinned
Jose Maldonado, PhD
@josemald.bsky.social
· Apr 11
Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday
Reposted by Jose Maldonado, PhD
"Rising from Chaos" #MicroscopyMonday: an active system of microtubules, kinesin motors and actin filaments.
🔬: Resonant Scanning Nikon AX-R Confocal
🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social
🏛️: www.brandeis.edu/science/reso...
📩: info@nesmicroscopy.org
🔬: Resonant Scanning Nikon AX-R Confocal
🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social
🏛️: www.brandeis.edu/science/reso...
📩: info@nesmicroscopy.org
April 28, 2025 at 2:25 PM
"Rising from Chaos" #MicroscopyMonday: an active system of microtubules, kinesin motors and actin filaments.
🔬: Resonant Scanning Nikon AX-R Confocal
🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social
🏛️: www.brandeis.edu/science/reso...
📩: info@nesmicroscopy.org
🔬: Resonant Scanning Nikon AX-R Confocal
🥼: Quang Tran of the Fraden Lab, MRSEC, @brandeisuniversity.bsky.social
🏛️: www.brandeis.edu/science/reso...
📩: info@nesmicroscopy.org
Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday
April 11, 2025 at 3:20 PM
Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday
Thank you to the team at @syGlass for highlighting some of our data. If you haven’t tried working with your data in VR space, you really need to see what you are missing. Syglass is a powerful tool for visualizing and analysis. @vubasicsciences.bsky.social #microscopy #lightsheet #science 🧪
Happy #3DThursday! Today we'll explore a SUB(!!)-volume of 500GB from a cleared, intact mouse brain from Jose Maldonado at Vanderbilt. You can see how we render these enormous data on consumer grade GPUs. 🥽💻
🔎 @josemald.bsky.social, @vucellimaging.bsky.social
🔬 lifecanvastech.com
🥽 syglass.io
🔎 @josemald.bsky.social, @vucellimaging.bsky.social
🔬 lifecanvastech.com
🥽 syglass.io
March 20, 2025 at 12:43 PM
Thank you to the team at @syGlass for highlighting some of our data. If you haven’t tried working with your data in VR space, you really need to see what you are missing. Syglass is a powerful tool for visualizing and analysis. @vubasicsciences.bsky.social #microscopy #lightsheet #science 🧪
Reposted by Jose Maldonado, PhD
I'm pleased to report that the board has now heard from NIH that the appointments of these three investigators and similarly situated folks are being extended. Thank you NIH! Delighted that the stellar work there will be continuing!
Compelling pushback from @jmgrohneuro.bsky.social and the Board of Scientific Counselors at the National Institute on Mental Health (NIMH) regarding termination of tenure-track investigators driven by DOGE.
1/2
1/2
March 6, 2025 at 10:25 PM
I'm pleased to report that the board has now heard from NIH that the appointments of these three investigators and similarly situated folks are being extended. Thank you NIH! Delighted that the stellar work there will be continuing!
Here is a closeup on our survey of the #hippocampus from Joe Luchsinger’s mouse sample, using the 15x objective from LifeCanvas Technologies Inc. With #lightsheet imaging you can peel away layers and observe cellular anatomy with stunning detail. How can VNL help you image your sample?
February 5, 2025 at 6:58 PM
Here is a closeup on our survey of the #hippocampus from Joe Luchsinger’s mouse sample, using the 15x objective from LifeCanvas Technologies Inc. With #lightsheet imaging you can peel away layers and observe cellular anatomy with stunning detail. How can VNL help you image your sample?
Reposted by Jose Maldonado, PhD
For #FluorescenceFriday, a side-by-side comparison of 5xFAD hemibrains at 6 months showing how Aβ plaques can be modulated not just by sex but also by the transgene parentage.
More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇
More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇
January 31, 2025 at 3:27 PM
For #FluorescenceFriday, a side-by-side comparison of 5xFAD hemibrains at 6 months showing how Aβ plaques can be modulated not just by sex but also by the transgene parentage.
More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇
More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇
Just a gorgeous #Purkinje neuron all by itself in the cerebellum. Only with #lightsheet #microscopy on a whole #cleared sample could you ever hope to catch a lone reporter expressing cell in it’s entirety. #science 🧪
January 28, 2025 at 11:39 PM
Just a gorgeous #Purkinje neuron all by itself in the cerebellum. Only with #lightsheet #microscopy on a whole #cleared sample could you ever hope to catch a lone reporter expressing cell in it’s entirety. #science 🧪
Definitely need to try this. Has anyone here used this on lightsheet meso scale data? @microscopy 🧪
Microscopy Nodes is now up on bioRxiv! 🚀
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...
January 20, 2025 at 3:04 AM
Definitely need to try this. Has anyone here used this on lightsheet meso scale data? @microscopy 🧪
Ok here’s a first go at looking at the previously acquired sample in 3D space. Still some work to do before we have a final product. 🧪
January 17, 2025 at 8:44 PM
Ok here’s a first go at looking at the previously acquired sample in 3D space. Still some work to do before we have a final product. 🧪
Getting closer… 🧪
January 16, 2025 at 7:57 PM
Getting closer… 🧪
Image preprocessing. This is from the acquisition in my previous post. Final image will be gorgeous!
January 16, 2025 at 5:03 PM
Image preprocessing. This is from the acquisition in my previous post. Final image will be gorgeous!
Thai is going to be a good one! Transgenic expression of tdTomato always yields phenomenal signal to noise.
January 13, 2025 at 5:43 PM
Thai is going to be a good one! Transgenic expression of tdTomato always yields phenomenal signal to noise.
Proud to have my images incorporated into this showcase video
Vanderbilt School of Medicine Basic Sciences Past, Present, and Future
YouTube video by VU School of Medicine Basic Sciences
youtu.be
January 10, 2025 at 4:28 PM
Proud to have my images incorporated into this showcase video
Video games grow with their audience.
Apparently one of the Yakuza games has an Alzheimer's Disease sub plot
Structural MRI in the mainstream 🧪🧠
Structural MRI in the mainstream 🧪🧠
December 7, 2024 at 7:15 PM
Video games grow with their audience.
And the Rocky Mountains just shrug it off.
Always love these animations of up and down land movement from earthquakes. Here's yesterdays. Great job @earthscope-sci.bsky.social 🧪
Watch the waves from the M7.0 earthquake in Northern California roll across seismic stations in North America.
🧵 THREAD
🧵 THREAD
December 6, 2024 at 5:33 PM
And the Rocky Mountains just shrug it off.
All the paint is still fresh for sharks.
Sharks (~440 million years) have been around much longer than Saturn's rings (~100 million years). #Science #Astronomy #Evolution #Biology 🧪🔭
December 5, 2024 at 3:56 PM
All the paint is still fresh for sharks.
Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪
December 4, 2024 at 2:02 AM
Lightsheet microscopy is unparalleled in its ability to resolve whole circuits in the mouse brain. Michelle Bedebaugh let me image one of her Rabies MC3R Cre samples with the 15X objective on my smartSPIM and we are very happy with the results. 🧪
Reposted by Jose Maldonado, PhD
There has been enough interest that we are on to Microscopy Starter Pack #2. Still open to who to include, including yourself!
go.bsky.app/DA4LAjJ
go.bsky.app/DA4LAjJ
November 22, 2024 at 3:15 PM
There has been enough interest that we are on to Microscopy Starter Pack #2. Still open to who to include, including yourself!
go.bsky.app/DA4LAjJ
go.bsky.app/DA4LAjJ
Reposted by Jose Maldonado, PhD
Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵Your weekend read👇
@cellpress.bsky.social
cell.com/cell-host-mi...
@cellpress.bsky.social
cell.com/cell-host-mi...
November 29, 2024 at 4:01 PM
Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵Your weekend read👇
@cellpress.bsky.social
cell.com/cell-host-mi...
@cellpress.bsky.social
cell.com/cell-host-mi...
Big Brother says you have nothing to worry about.
Nearly half of US states grant law enforcement access to testosterone prescription data within Prescription Drug Monitoring Programs (PDMPs) without warrant or subpoena.
https://ja.ma/4eVwQvk
https://ja.ma/4eVwQvk
November 29, 2024 at 9:09 PM
Big Brother says you have nothing to worry about.
Reposted by Jose Maldonado, PhD
Here we show how #microglia contact #pericytes and #endothelialcells in the #venule end of the #capillary bed. Note also how a #primarycilium of an #interneuron invaginates the microglia. But why? 🤔 journals.sagepub.com/doi/full/10.... @gazz4science.bsky.social
November 26, 2024 at 5:44 PM
Here we show how #microglia contact #pericytes and #endothelialcells in the #venule end of the #capillary bed. Note also how a #primarycilium of an #interneuron invaginates the microglia. But why? 🤔 journals.sagepub.com/doi/full/10.... @gazz4science.bsky.social
Lightsheet for the win! 🧪
Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics - www.nature.com/articles/s44...
Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics - npj Imaging
npj Imaging - Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics
www.nature.com
November 25, 2024 at 8:13 PM
Lightsheet for the win! 🧪
I love good data visualization
NASA's visualization team created a preview of every phase of the Moon in 2025, hour by hour.
The best part: Watching how the Moon's appearance changes due to its inclination, oval orbit, and libration (wobble). 🔭🧪
svs.gsfc.nasa.gov/5415
The best part: Watching how the Moon's appearance changes due to its inclination, oval orbit, and libration (wobble). 🔭🧪
svs.gsfc.nasa.gov/5415
November 24, 2024 at 9:48 PM
I love good data visualization
Reposted by Jose Maldonado, PhD
Hard to overstate how pleased I am that the FlyWire consortium converted our FAFB ("Full Adult Fly Brain") EM volume into a connectome, and that the resulting science has been (and will be) so impactful. Thanks to everyone involved. #neuroscience www.nature.com/immersive/d4...
The FlyWire connectome: neuronal wiring diagram of a complete fly brain
Artificial intelligence and human expertise meet to generate a map of all the connections in the fly brain. The resource is already being used by experimentalists and theoreticians to further our unde...
www.nature.com
October 2, 2024 at 7:08 PM
Hard to overstate how pleased I am that the FlyWire consortium converted our FAFB ("Full Adult Fly Brain") EM volume into a connectome, and that the resulting science has been (and will be) so impactful. Thanks to everyone involved. #neuroscience www.nature.com/immersive/d4...