Guido Puccetti
@guidopuccetti.bsky.social
Pinned
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen | mBio
Sustainable food production requires the management of disease agents attacking crops.
Application of antifungal compounds is among the key elements of pathogen containment;
however, resistance can ri...
journals.asm.org
Finally out!
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
Reposted by Guido Puccetti
k-mer-based GWAS reveals a candidate avirulence gene and structural variation in Puccinia triticina linked to gain of Lr20 virulence doi.org/10.1186/s128...
December 1, 2025 at 9:50 PM
k-mer-based GWAS reveals a candidate avirulence gene and structural variation in Puccinia triticina linked to gain of Lr20 virulence doi.org/10.1186/s128...
Reposted by Guido Puccetti
www.embopress.org/doi/full/10....
Our latest now online at EMBO Journal. Read if you are interested in how epimutations mediate antifungal resistance & how this might result in heteroresistance in human & cereal crop fungal pathogens. Big Thx & congrats to Andreas Fellas, Pin Tong & Alison Pidoux
Our latest now online at EMBO Journal. Read if you are interested in how epimutations mediate antifungal resistance & how this might result in heteroresistance in human & cereal crop fungal pathogens. Big Thx & congrats to Andreas Fellas, Pin Tong & Alison Pidoux
Heterochromatin epimutations impose mitochondrial dysfunction to confer antifungal resistance | The EMBO Journal
imageimageHeterochromatin-island epimutations can provide resistance to caffeine and antifungal
drugs in the fission yeast Schizosaccharomyces pombe. This study reveals that some epimutations cause re...
www.embopress.org
December 1, 2025 at 3:44 PM
www.embopress.org/doi/full/10....
Our latest now online at EMBO Journal. Read if you are interested in how epimutations mediate antifungal resistance & how this might result in heteroresistance in human & cereal crop fungal pathogens. Big Thx & congrats to Andreas Fellas, Pin Tong & Alison Pidoux
Our latest now online at EMBO Journal. Read if you are interested in how epimutations mediate antifungal resistance & how this might result in heteroresistance in human & cereal crop fungal pathogens. Big Thx & congrats to Andreas Fellas, Pin Tong & Alison Pidoux
Finally out!
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen | mBio
Sustainable food production requires the management of disease agents attacking crops.
Application of antifungal compounds is among the key elements of pathogen containment;
however, resistance can ri...
journals.asm.org
December 1, 2025 at 12:00 AM
Finally out!
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen
journals.asm.org/doi/10.1128/...
A big thanks to @danielcroll.bsky.social & @GabrielScalliet
#azole #DMI #GWAS #fungicide #resistance #zymoseptoria #Europe
Reposted by Guido Puccetti
Our paper on Zymoseptoria tritici 3D genome organization is officially out! @iglavincheska.bsky.social
link.springer.com/article/10.1...
link.springer.com/article/10.1...
Three-dimensional genome architecture connects chromatin structure and function in a major wheat pathogen - BMC Biology
Background Genome spatial organization plays a fundamental role in biological function across all domains of life. While the principles of nuclear architecture have been well-characterized in animals ...
link.springer.com
November 28, 2025 at 12:43 PM
Our paper on Zymoseptoria tritici 3D genome organization is officially out! @iglavincheska.bsky.social
link.springer.com/article/10.1...
link.springer.com/article/10.1...
Reposted by Guido Puccetti
Cross-kingdom RNA trafficking from bacteria to fungi enables plant protection against fungal pathogens #research #MolecularPlant cell.com/molecular-pl...
November 15, 2025 at 12:51 PM
Cross-kingdom RNA trafficking from bacteria to fungi enables plant protection against fungal pathogens #research #MolecularPlant cell.com/molecular-pl...
Reposted by Guido Puccetti
Over many years, and a pandemic, we have worked with an international team of researchers to answer the question through genomics. We believe the origin is recent (in the last few decades, in line with previous research) and spatially from Western Europe
November 13, 2025 at 12:45 PM
Over many years, and a pandemic, we have worked with an international team of researchers to answer the question through genomics. We believe the origin is recent (in the last few decades, in line with previous research) and spatially from Western Europe
Reposted by Guido Puccetti
TEs aren’t just genomic parasites, they’re also engines of genomic novelty.
Our new study with ~2,000 Z. tritici genomes shows repeated TE mobilization waves during global expansion.
With @danielcroll.bsky.social & @guidopuccetti.bsky.social
🧬 www.nature.com/articles/s41...
#TEworldwide
Our new study with ~2,000 Z. tritici genomes shows repeated TE mobilization waves during global expansion.
With @danielcroll.bsky.social & @guidopuccetti.bsky.social
🧬 www.nature.com/articles/s41...
#TEworldwide
Historic transposon mobilisation waves create distinct pools of adaptive variants in a major crop pathogen - Nature Communications
In this study, the authors analysed a large genomic dataset to trace how jumping genes shaped the global spread of a major wheat pathogen and reveal bursts of activity over decades that drove adaptati...
www.nature.com
November 12, 2025 at 1:17 PM
TEs aren’t just genomic parasites, they’re also engines of genomic novelty.
Our new study with ~2,000 Z. tritici genomes shows repeated TE mobilization waves during global expansion.
With @danielcroll.bsky.social & @guidopuccetti.bsky.social
🧬 www.nature.com/articles/s41...
#TEworldwide
Our new study with ~2,000 Z. tritici genomes shows repeated TE mobilization waves during global expansion.
With @danielcroll.bsky.social & @guidopuccetti.bsky.social
🧬 www.nature.com/articles/s41...
#TEworldwide
Reposted by Guido Puccetti
Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus journals.plos.org/plosgenetics...
Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus
Author summary Cryptococcus is a genus of fungi that includes both pathogenic species capable of causing life-threatening infections in humans and many environmental species that inhabit soil, fruit, ...
journals.plos.org
November 11, 2025 at 10:35 AM
Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus journals.plos.org/plosgenetics...
Reposted by Guido Puccetti
Lifestyles shape genome size and gene content in fungal pathogens.
buff.ly/tec1Tds
buff.ly/tec1Tds
November 2, 2025 at 11:01 AM
Lifestyles shape genome size and gene content in fungal pathogens.
buff.ly/tec1Tds
buff.ly/tec1Tds
Reposted by Guido Puccetti
Still using 16S/ITS profiling? You might want to reconsider👀
Our new paper presents pangenome-informed amplicons that provide up to 10× higher phylogenetic resolution than full-length ribosomal markers- while remaining cost effective and scalable!
microbiomejournal.biomedcentral.com/articles/10....
Our new paper presents pangenome-informed amplicons that provide up to 10× higher phylogenetic resolution than full-length ribosomal markers- while remaining cost effective and scalable!
microbiomejournal.biomedcentral.com/articles/10....
High-resolution profiling of bacterial and fungal communities using pangenome-informed taxon-specific long-read amplicons - Microbiome
Background High-throughput sequencing technologies have greatly advanced our understanding of microbiomes, but resolving microbial communities at species and strain levels remains challenging. Results...
microbiomejournal.biomedcentral.com
October 30, 2025 at 1:32 PM
Still using 16S/ITS profiling? You might want to reconsider👀
Our new paper presents pangenome-informed amplicons that provide up to 10× higher phylogenetic resolution than full-length ribosomal markers- while remaining cost effective and scalable!
microbiomejournal.biomedcentral.com/articles/10....
Our new paper presents pangenome-informed amplicons that provide up to 10× higher phylogenetic resolution than full-length ribosomal markers- while remaining cost effective and scalable!
microbiomejournal.biomedcentral.com/articles/10....
Reposted by Guido Puccetti
WHY USE #YEAST AS A TOOL WHEN YOU'VE GOT STAGO?😁
Out now on @biorxivpreprint.bsky.social, our collab @theccdm.bsky.social led by @labkatz.bsky.social and Fran Lopez-Ruiz developed Parastagonospora nodorum as a tool to study #fungicideresistance genes.
www.biorxiv.org/content/10.1...
Out now on @biorxivpreprint.bsky.social, our collab @theccdm.bsky.social led by @labkatz.bsky.social and Fran Lopez-Ruiz developed Parastagonospora nodorum as a tool to study #fungicideresistance genes.
www.biorxiv.org/content/10.1...
Dissecting allele-specific fungicide resistance mechanisms by heterologous expression of the demethylase inhibitor target gene Cyp51 in a phytopathogen model
BACKGROUND Fungicide resistance is a major concern both in agriculture and clinical disease control. Whilst several mechanisms of resistance have been elucidated, assigning phenotype to genotype is of...
www.biorxiv.org
October 22, 2025 at 1:37 AM
WHY USE #YEAST AS A TOOL WHEN YOU'VE GOT STAGO?😁
Out now on @biorxivpreprint.bsky.social, our collab @theccdm.bsky.social led by @labkatz.bsky.social and Fran Lopez-Ruiz developed Parastagonospora nodorum as a tool to study #fungicideresistance genes.
www.biorxiv.org/content/10.1...
Out now on @biorxivpreprint.bsky.social, our collab @theccdm.bsky.social led by @labkatz.bsky.social and Fran Lopez-Ruiz developed Parastagonospora nodorum as a tool to study #fungicideresistance genes.
www.biorxiv.org/content/10.1...
Reposted by Guido Puccetti
Fungal #GenomeSize variation is largely driven by TEs & lineage-specific genome defenses. @tbadet.bsky.social & @danielcroll.bsky.social show that presence/absence of RIP mutation systems influences #genome architecture, identifying a likely novel defense mechanism @plosbiology.org 🧪 plos.io/4hcC5tx
October 16, 2025 at 7:58 AM
Fungal #GenomeSize variation is largely driven by TEs & lineage-specific genome defenses. @tbadet.bsky.social & @danielcroll.bsky.social show that presence/absence of RIP mutation systems influences #genome architecture, identifying a likely novel defense mechanism @plosbiology.org 🧪 plos.io/4hcC5tx
Reposted by Guido Puccetti
New preprint reveals bacteria can't just collect all resistance genes like Pokemon cards.
We found mutually exclusive evolutionary pathways to multidrug resistance in E. coli & P. aeruginosa - some resistance mechanisms actively prevent others from coexisting www.biorxiv.org/content/10.1...
We found mutually exclusive evolutionary pathways to multidrug resistance in E. coli & P. aeruginosa - some resistance mechanisms actively prevent others from coexisting www.biorxiv.org/content/10.1...
Genomic constraints shape the evolution of alternative routes to drug resistance in prokaryotes
Background Variation within the prokaryotic pangenome is not random, and natural selection that favours particular combinations of genes appears to dominate over random drift. What is less clear is wh...
www.biorxiv.org
August 29, 2025 at 2:05 PM
New preprint reveals bacteria can't just collect all resistance genes like Pokemon cards.
We found mutually exclusive evolutionary pathways to multidrug resistance in E. coli & P. aeruginosa - some resistance mechanisms actively prevent others from coexisting www.biorxiv.org/content/10.1...
We found mutually exclusive evolutionary pathways to multidrug resistance in E. coli & P. aeruginosa - some resistance mechanisms actively prevent others from coexisting www.biorxiv.org/content/10.1...
Reposted by Guido Puccetti
I thought the Aspergillus fumigatus pan-genome was pretty wild when we started this project.
Aspergillus flavus said, "hold my beer..." Not only does it have a strong population structure with differences between environmental and clinical strains, only 58%of genes are conserved across the species!
Aspergillus flavus said, "hold my beer..." Not only does it have a strong population structure with differences between environmental and clinical strains, only 58%of genes are conserved across the species!
Thrilled to share that the final chapter of my PhD with @rokaslab.bsky.social is now out in @natcomms.nature.com 🎉
Read it to find out how population structure impacts pathogenicity in Aspergillus flavus, a fungus that infects humans 🧪
rdcu.be/eAToj
Read it to find out how population structure impacts pathogenicity in Aspergillus flavus, a fungus that infects humans 🧪
rdcu.be/eAToj
Population structure in a fungal human pathogen is potentially linked to pathogenicity
Nature Communications - Genetic diversity between clinical and environmental fungal isolates of Aspergillus flavus is poorly studied. Here, the authors analysed genomic data from a global set of...
rdcu.be
August 22, 2025 at 6:38 AM
I thought the Aspergillus fumigatus pan-genome was pretty wild when we started this project.
Aspergillus flavus said, "hold my beer..." Not only does it have a strong population structure with differences between environmental and clinical strains, only 58%of genes are conserved across the species!
Aspergillus flavus said, "hold my beer..." Not only does it have a strong population structure with differences between environmental and clinical strains, only 58%of genes are conserved across the species!
Reposted by Guido Puccetti
🚨 Fungi + viruses + mammalian lungs? Buckle up! Our new paper in
@natmicrobiol.nature.com
uncovers the story of a deadly fungus and its gnarly viral hitchhiker — and how this duo may change how we diagnose & treat fungal disease 🍄🫁🚨 doi.org/10.1038/s415... ⬇️
@natmicrobiol.nature.com
uncovers the story of a deadly fungus and its gnarly viral hitchhiker — and how this duo may change how we diagnose & treat fungal disease 🍄🫁🚨 doi.org/10.1038/s415... ⬇️
Aspergillus fumigatus dsRNA virus promotes fungal fitness and pathogenicity in the mammalian host - Nature Microbiology
A mycovirus infecting the pathogenic fungus Aspergillus fumigatus enhances its stress tolerance and virulence in mice.
doi.org
August 14, 2025 at 9:46 AM
🚨 Fungi + viruses + mammalian lungs? Buckle up! Our new paper in
@natmicrobiol.nature.com
uncovers the story of a deadly fungus and its gnarly viral hitchhiker — and how this duo may change how we diagnose & treat fungal disease 🍄🫁🚨 doi.org/10.1038/s415... ⬇️
@natmicrobiol.nature.com
uncovers the story of a deadly fungus and its gnarly viral hitchhiker — and how this duo may change how we diagnose & treat fungal disease 🍄🫁🚨 doi.org/10.1038/s415... ⬇️
Reposted by Guido Puccetti
A little holiday news:
Evolution of antifungal resistance in the environment www.nature.com/articles/s41...
This started out as a little thought way back in early 2024 between @normanvanrhijn.bsky.social and I, and turned into a full on review! We hope it proves useful to the community.
Evolution of antifungal resistance in the environment www.nature.com/articles/s41...
This started out as a little thought way back in early 2024 between @normanvanrhijn.bsky.social and I, and turned into a full on review! We hope it proves useful to the community.
Evolution of antifungal resistance in the environment - Nature Microbiology
This Review discusses the evolution, emergence and expansion of environmental fungicide and antifungal drug resistance.
www.nature.com
July 29, 2025 at 11:29 AM
A little holiday news:
Evolution of antifungal resistance in the environment www.nature.com/articles/s41...
This started out as a little thought way back in early 2024 between @normanvanrhijn.bsky.social and I, and turned into a full on review! We hope it proves useful to the community.
Evolution of antifungal resistance in the environment www.nature.com/articles/s41...
This started out as a little thought way back in early 2024 between @normanvanrhijn.bsky.social and I, and turned into a full on review! We hope it proves useful to the community.
Reposted by Guido Puccetti
A pangenomics-enabled platform for the high-throughput discovery of antifungal resistance factors in crop pathogens https://www.biorxiv.org/content/10.1101/2025.07.18.665620v1
July 20, 2025 at 4:17 AM
A pangenomics-enabled platform for the high-throughput discovery of antifungal resistance factors in crop pathogens https://www.biorxiv.org/content/10.1101/2025.07.18.665620v1
Reposted by Guido Puccetti
We dive into the dynamics of #starships 🚀 in a fungal pathogen to ask: how might these giant #transposons impact human health? We find they drive genome-wide variation, encode clinically-relevant traits and even transpose within the same strain! 🍄🧪 out today in mBio #goteam doi.org/10.1128/mbio...
Giant transposons promote strain heterogeneity in a major fungal pathogen | mBio
No “one size fits all” option exists for treating fungal infections in large part
due to genetic and phenotypic variability among strains. Accounting for strain heterogeneity
is thus fundamental for d...
doi.org
May 13, 2025 at 8:11 AM
We dive into the dynamics of #starships 🚀 in a fungal pathogen to ask: how might these giant #transposons impact human health? We find they drive genome-wide variation, encode clinically-relevant traits and even transpose within the same strain! 🍄🧪 out today in mBio #goteam doi.org/10.1128/mbio...
Reposted by Guido Puccetti
Super happy to see it out with all the hard work of everyone ! Such a nice story ! 🔥
Our preprint has now found a wonderful home in @molbioevol.bsky.social. Shout out to our collaborators in the Mozgova lab too 💚
New study with @petrollromy.bsky.social @borglab.bsky.social traces the evolution of epigenetic silencing in plants. Deep conservation of PRC2-mediated H3K27me3 marks across green algae suggest ancient, conserved networks of gene repression.
🔗 doi.org/10.1093/molb...
#evobio #molevol #plants
🔗 doi.org/10.1093/molb...
#evobio #molevol #plants
April 20, 2025 at 9:25 AM
Super happy to see it out with all the hard work of everyone ! Such a nice story ! 🔥
Reposted by Guido Puccetti
It's been so many months that I have been postponing the publication of my poster's comics since I wanted to translate it (which I did not officially...), so why not doing it on the Labour Day ? 😅 It is now on Zenodo (French): doi.org/10.5281/zeno... 🧪🐡🎨
(1/2)
(1/2)
Les Aventures de Zymo: un champignon mangeur de blé
Ce poster comprend quatre pages de bande dessinée, originalement créée pour ma famille, et n’a d’autres prétentions que d’ouvrir à la discussion sur ce champignon pathogène du blé, Zymoseptoria tritic...
doi.org
May 1, 2025 at 1:44 PM
It's been so many months that I have been postponing the publication of my poster's comics since I wanted to translate it (which I did not officially...), so why not doing it on the Labour Day ? 😅 It is now on Zenodo (French): doi.org/10.5281/zeno... 🧪🐡🎨
(1/2)
(1/2)
Reposted by Guido Puccetti
Very excited with @tobybarilbio.bsky.social to share this preprint about his latest work on TEs!
We knew that TEs were very pretty active in this global fungal pathogen of wheat.
Now with nearly 2000 Illumina genomes available, we could finally ask questions about historic TE activation waves.
We knew that TEs were very pretty active in this global fungal pathogen of wheat.
Now with nearly 2000 Illumina genomes available, we could finally ask questions about historic TE activation waves.
April 9, 2025 at 3:21 PM
Very excited with @tobybarilbio.bsky.social to share this preprint about his latest work on TEs!
We knew that TEs were very pretty active in this global fungal pathogen of wheat.
Now with nearly 2000 Illumina genomes available, we could finally ask questions about historic TE activation waves.
We knew that TEs were very pretty active in this global fungal pathogen of wheat.
Now with nearly 2000 Illumina genomes available, we could finally ask questions about historic TE activation waves.
Reposted by Guido Puccetti
🔥 Exciting news! The first Symposium on Repeat-Induced Point mutations is happening June 30 – July 1 at the University of Neuchâtel🇨🇭!
🔹 Want to join? Register here > forms.gle/XWTd4gJAXZSx...
🚨 Limited spots available! So first come, first served!
🔹 More info > crolllab.github.io/ripm2025/
🔹 Want to join? Register here > forms.gle/XWTd4gJAXZSx...
🚨 Limited spots available! So first come, first served!
🔹 More info > crolllab.github.io/ripm2025/
RIPM 2025
Symposium on Repeat-induced Point Mutation research, 30.6 - 1.7, Neuchatel, Switzerland
crolllab.github.io
March 28, 2025 at 9:47 AM
🔥 Exciting news! The first Symposium on Repeat-Induced Point mutations is happening June 30 – July 1 at the University of Neuchâtel🇨🇭!
🔹 Want to join? Register here > forms.gle/XWTd4gJAXZSx...
🚨 Limited spots available! So first come, first served!
🔹 More info > crolllab.github.io/ripm2025/
🔹 Want to join? Register here > forms.gle/XWTd4gJAXZSx...
🚨 Limited spots available! So first come, first served!
🔹 More info > crolllab.github.io/ripm2025/
Reposted by Guido Puccetti
@guidopuccetti.bsky.social, Gabriel Scalliet and I are excited to share our latest work.
Resistance in agriculture emerges fast, but we fail to grasp how the myriad mutations in pathogen populations contribute to this.
Please check out and share our latest preprint: www.biorxiv.org/content/10.1...
Resistance in agriculture emerges fast, but we fail to grasp how the myriad mutations in pathogen populations contribute to this.
Please check out and share our latest preprint: www.biorxiv.org/content/10.1...
March 25, 2025 at 10:05 PM
@guidopuccetti.bsky.social, Gabriel Scalliet and I are excited to share our latest work.
Resistance in agriculture emerges fast, but we fail to grasp how the myriad mutations in pathogen populations contribute to this.
Please check out and share our latest preprint: www.biorxiv.org/content/10.1...
Resistance in agriculture emerges fast, but we fail to grasp how the myriad mutations in pathogen populations contribute to this.
Please check out and share our latest preprint: www.biorxiv.org/content/10.1...
Reposted by Guido Puccetti
A large European diversity panel reveals complex azole fungicide resistance gains of a major wheat pathogen https://www.biorxiv.org/content/10.1101/2025.03.19.644200v1
March 20, 2025 at 8:14 AM
Reposted by Guido Puccetti
Our paper on the evolution of fungicide resistance in wheat powdery mildew is now published! Congrats to all coauthors! doi.org/10.1111/mpp....
Genomic Surveillance and Molecular Evolution of Fungicide Resistance in European Populations of Wheat Powdery Mildew
Mutations conferring fungicide resistance to wheat powdery mildew emerged multiple times and are characterised by distinct geographic ranges in Europe and neighbouring regions.
doi.org
March 20, 2025 at 8:51 AM
Our paper on the evolution of fungicide resistance in wheat powdery mildew is now published! Congrats to all coauthors! doi.org/10.1111/mpp....