Satpal (Sati) Jhujh
@drjhujh.bsky.social
Interested in all things DNA Replication, DNA repair and human disease!
Pinned
Satpal (Sati) Jhujh
@drjhujh.bsky.social
· Nov 15
Reposted by Satpal (Sati) Jhujh
How do SMC complexes capture DNA? 🧪
Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.
Read more: tinyurl.com/4s8nrrkk
#LMBResearch
Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.
Read more: tinyurl.com/4s8nrrkk
#LMBResearch
March 31, 2025 at 2:40 PM
How do SMC complexes capture DNA? 🧪
Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.
Read more: tinyurl.com/4s8nrrkk
#LMBResearch
Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.
Read more: tinyurl.com/4s8nrrkk
#LMBResearch
Reposted by Satpal (Sati) Jhujh
Excited to share our discovery of the RAD51 filament cap, built by a newly identified RAD51 paralog complex 🔬🧬
‼️ Excited to share our new paper out now in @science.org ‼️
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
We describe a new tetrameric RAD51 paralog complex – XRCC3-RAD51C-RAD51D-XRCC2 – which caps the end of RAD51 filaments.
Link: www.science.org/doi/epdf/10....
Thread ⬇️ (1/8)
November 7, 2025 at 10:22 AM
Excited to share our discovery of the RAD51 filament cap, built by a newly identified RAD51 paralog complex 🔬🧬
Reposted by Satpal (Sati) Jhujh
New preprint from the lab for any DNA repair or replication stress geeks out there!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A nuclease-driven mechanism of post-replicative ssDNA gap suppression.
The persistence of post-replicative ssDNA gaps following PRIMPOL-mediated replication repriming is linked to chemosensitivity, and in all models reported to date the nuclease MRE11 has been implicated...
www.biorxiv.org
October 22, 2025 at 6:56 PM
New preprint from the lab for any DNA repair or replication stress geeks out there!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Satpal (Sati) Jhujh
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
www.science.org
October 16, 2025 at 7:17 PM
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Satpal (Sati) Jhujh
🧬 Preprint alert!
"Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity."
Read the manuscript here 👉 www.biorxiv.org/content/10.1...
"Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity."
Read the manuscript here 👉 www.biorxiv.org/content/10.1...
Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Mutations in the ATRX chromatin remodeller predispose to a developmental genetic disorder and cancer, but how it safeguards genome and telomere stability remains unresolved. Here, we uncover critical ...
www.biorxiv.org
October 7, 2025 at 9:49 PM
🧬 Preprint alert!
"Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity."
Read the manuscript here 👉 www.biorxiv.org/content/10.1...
"Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity."
Read the manuscript here 👉 www.biorxiv.org/content/10.1...
Reposted by Satpal (Sati) Jhujh
"… as I helped this bright young scholar rediscover hope in her journey, I rediscovered my own." #ScienceWorkingLife https://scim.ag/473CI43
September 30, 2025 at 1:29 PM
"… as I helped this bright young scholar rediscover hope in her journey, I rediscovered my own." #ScienceWorkingLife https://scim.ag/473CI43
Reposted by Satpal (Sati) Jhujh
Our preprint on Pol-η usage during genome replication has been uploaded. It addresses the biased use of Pol-η in the lagging strand and late-replicating regions, which may be correlated with cancer mutagenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Strand- and replication timing-dependent functions of DNA polymerase η in human DNA replication and mutagenesis.
Efficient eukaryotic DNA replication relies on the coordinated actions of replicative and error-prone polymerases, with the latter providing flexibility at the cost of mutagenesis. DNA polymerase η (P...
www.biorxiv.org
September 12, 2025 at 8:46 AM
Our preprint on Pol-η usage during genome replication has been uploaded. It addresses the biased use of Pol-η in the lagging strand and late-replicating regions, which may be correlated with cancer mutagenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Satpal (Sati) Jhujh
DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis https://www.biorxiv.org/content/10.1101/2025.09.09.675193v1
September 10, 2025 at 1:17 PM
DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis https://www.biorxiv.org/content/10.1101/2025.09.09.675193v1
Reposted by Satpal (Sati) Jhujh
New preprint with @gfudenberg.bsky.social
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
NIPBL dosage shapes genome folding by tuning the rate of cohesin loop extrusion
Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...
www.biorxiv.org
August 16, 2025 at 3:03 AM
New preprint with @gfudenberg.bsky.social
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
Reposted by Satpal (Sati) Jhujh
My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social
DNA2 enables growth by restricting recombination-restarted replication - Nature
DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...
www.nature.com
September 3, 2025 at 3:59 PM
My first post on bsky to share our latest research in @nature.com: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators & @ukri.org @acmedsci.bsky.social
Reposted by Satpal (Sati) Jhujh
Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...
May 15, 2025 at 12:55 AM
Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...
Reposted by Satpal (Sati) Jhujh
Slow RNAPII elongation enhances naive-pluripotency rewiring while preserving replication fork speed https://www.biorxiv.org/content/10.1101/2025.05.12.653265v1
May 15, 2025 at 9:18 PM
Slow RNAPII elongation enhances naive-pluripotency rewiring while preserving replication fork speed https://www.biorxiv.org/content/10.1101/2025.05.12.653265v1
Reposted by Satpal (Sati) Jhujh
3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴
www.biochemistry.org/events-and-t...
www.biochemistry.org/events-and-t...
UK DNA Replication Meeting 2026
Research in the DNA replication field is progressing at a remarkable rate. Technological innovations in biochemical reconstitution, structural biology, proteomics and DNA sequencing are helping to rap...
www.biochemistry.org
April 15, 2025 at 10:30 AM
3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴
www.biochemistry.org/events-and-t...
www.biochemistry.org/events-and-t...
🎉 Congratulations! Great to see this out in print! 👏
📢 Just published! Excited to share my latest research, now published in Nature Communications! Grateful to my collaborators and mentors who made this work possible.
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
@profstewartlab.bsky.social @Jo Morris, @Aneika C. Leney, @drjhujh.bsky.social
www.nature.com/articles/s41...
PIN1-SUMO2/3 motif suppresses excessive RNF168 chromatin accumulation and ubiquitin signaling to promote IR resistance - Nature Communications
The ubiquitin ligase, RNF168, promotes DNA break repair but must be regulated to prevent run-away ubiquitin signaling. Here, the authors identify a three-step post-translational cascade regulating RNF...
www.nature.com
April 14, 2025 at 2:40 PM
🎉 Congratulations! Great to see this out in print! 👏
Reposted by Satpal (Sati) Jhujh
Pls repost. Postdoc role on post-replicative ssDNA gap processing. Interesting models that should float replication aficionados boats. DM or c.staples@bangor.ac.uk for info. Until Dec26 but will strive for funding to provide continuity.
Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...
Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...
March 24, 2025 at 6:28 PM
Pls repost. Postdoc role on post-replicative ssDNA gap processing. Interesting models that should float replication aficionados boats. DM or c.staples@bangor.ac.uk for info. Until Dec26 but will strive for funding to provide continuity.
Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...
Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...
Reposted by Satpal (Sati) Jhujh
How are chromosome breaks repaired and how is their repair influenced and facilitated by nuclear and genome dynamics? Fantastic initiative and collaboration with @karimmekhail.bsky.social, @chiololab.bsky.social, and @lablegube.bsky.social. @natrevmcb.bsky.social Link to article: rdcu.be/edViW
Nuclear and genome dynamics underlying DNA double-strand break repair
Nature Reviews Molecular Cell Biology - Changes in nuclear and genome organization promote the repair of DNA double-strand breaks and genome stability. Processes that are involved include the...
rdcu.be
March 18, 2025 at 4:32 PM
How are chromosome breaks repaired and how is their repair influenced and facilitated by nuclear and genome dynamics? Fantastic initiative and collaboration with @karimmekhail.bsky.social, @chiololab.bsky.social, and @lablegube.bsky.social. @natrevmcb.bsky.social Link to article: rdcu.be/edViW
Reposted by Satpal (Sati) Jhujh
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
www.biorxiv.org
March 10, 2025 at 11:18 PM
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Satpal (Sati) Jhujh
TRIP13 protects pancreatic cancer cells against intrinsic and therapy-induced DNA replication stress https://www.biorxiv.org/content/10.1101/2025.01.26.634889v1
January 28, 2025 at 12:52 AM
TRIP13 protects pancreatic cancer cells against intrinsic and therapy-induced DNA replication stress https://www.biorxiv.org/content/10.1101/2025.01.26.634889v1
Reposted by Satpal (Sati) Jhujh
Telomeric SUMO level influences the choices of APB formation pathways and ALT efficiency https://www.biorxiv.org/content/10.1101/2025.01.16.633463v1
January 20, 2025 at 9:28 AM
Telomeric SUMO level influences the choices of APB formation pathways and ALT efficiency https://www.biorxiv.org/content/10.1101/2025.01.16.633463v1
Reposted by Satpal (Sati) Jhujh
Okay, the list is far from complete, but it is a first attempt.☺️Here you can find our starter pack on #research organizations and #academies, in #Germany and worldwide. Who else do you think should be included? go.bsky.app/DGGtVu6
January 16, 2025 at 1:56 PM
Okay, the list is far from complete, but it is a first attempt.☺️Here you can find our starter pack on #research organizations and #academies, in #Germany and worldwide. Who else do you think should be included? go.bsky.app/DGGtVu6
Reposted by Satpal (Sati) Jhujh
New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today:
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
January 16, 2025 at 4:11 PM
New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today:
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
1/
Reposted by Satpal (Sati) Jhujh
The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain https://www.biorxiv.org/content/10.1101/2025.01.10.632395v1
January 13, 2025 at 1:32 PM
The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain https://www.biorxiv.org/content/10.1101/2025.01.10.632395v1
Reposted by Satpal (Sati) Jhujh
Identification of critical factors of the replication stress response in human cells https://www.biorxiv.org/content/10.1101/2025.01.12.632149v1
January 12, 2025 at 10:30 PM
Identification of critical factors of the replication stress response in human cells https://www.biorxiv.org/content/10.1101/2025.01.12.632149v1
Reposted by Satpal (Sati) Jhujh
Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Cell cycle progression of under-replicated cells
Abstract. Cell cycle checkpoints are the regulatory mechanisms that secure the strict order of cellular events for cell division that ensure genome integri
academic.oup.com
January 10, 2025 at 5:42 PM
Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Reposted by Satpal (Sati) Jhujh
Great start to 2025. Tumor hypoxia promotes immune evasion by autophagy-mediated downregulation of MHC class I expression leading to reduced antigen presentation. Great work from @halaestephan.bsky.social www.embopress.org/doi/full/10....
Hypoxia promotes tumor immune evasion by suppressing MHC-I expression and antigen presentation | The EMBO Journal
imageimageHypoxia contributes to cancer resistance to both standard therapies and immunotherapy.
This work demonstrates that tumor hypoxia promotes immune evasion by autophagy-mediated
downregulation ...
www.embopress.org
January 3, 2025 at 9:30 AM
Great start to 2025. Tumor hypoxia promotes immune evasion by autophagy-mediated downregulation of MHC class I expression leading to reduced antigen presentation. Great work from @halaestephan.bsky.social www.embopress.org/doi/full/10....