James Dewar
@jamesmdewar.bsky.social
Assistant professor, Vanderbilt University School of Medicine. Studying DNA replication & repair to understand biochemical mechanisms and environmental toxicology.
Pinned
James Dewar
@jamesmdewar.bsky.social
· Mar 10
Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
www.biorxiv.org
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Awesome resource and super proud of Dewar lab alum tatakavlashvili.bsky.social who co-led the work.
March 11, 2025 at 9:35 PM
Awesome resource and super proud of Dewar lab alum tatakavlashvili.bsky.social who co-led the work.
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
www.biorxiv.org
March 10, 2025 at 11:18 PM
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
In our latest preprint we analyzed how abasic (AP) sites affect DNA replication:
- AP sites stall DNA synthesis ⛓️💥
- Leading APs stall replisomes but lagging APs don’t 🛑/✅
- TLS is crucial for bypass of both leading and lagging strand AP sites 🛠️🧬
www.biorxiv.org/content/10.1...
- AP sites stall DNA synthesis ⛓️💥
- Leading APs stall replisomes but lagging APs don’t 🛑/✅
- TLS is crucial for bypass of both leading and lagging strand AP sites 🛠️🧬
www.biorxiv.org/content/10.1...
Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms
Abasic sites are one of the most frequent forms of DNA damage that interfere with DNA replication. However, abasic sites exhibit complex effects because they can be processed into other types of DNA d...
www.biorxiv.org
January 13, 2025 at 6:47 PM
In our latest preprint we analyzed how abasic (AP) sites affect DNA replication:
- AP sites stall DNA synthesis ⛓️💥
- Leading APs stall replisomes but lagging APs don’t 🛑/✅
- TLS is crucial for bypass of both leading and lagging strand AP sites 🛠️🧬
www.biorxiv.org/content/10.1...
- AP sites stall DNA synthesis ⛓️💥
- Leading APs stall replisomes but lagging APs don’t 🛑/✅
- TLS is crucial for bypass of both leading and lagging strand AP sites 🛠️🧬
www.biorxiv.org/content/10.1...