Phil Kyriakakis
@breakliquid.bsky.social
Senior Research Scientist at Stanford Bioengineering | Taking apart and building biology.🧪
Is there an easy way to get saw RNA-seq data and turn it into a table of differentially expressed genes?
Like, without installing 12 programs and taking a bioinformatics course?
Like, without installing 12 programs and taking a bioinformatics course?
January 16, 2026 at 6:59 AM
Is there an easy way to get saw RNA-seq data and turn it into a table of differentially expressed genes?
Like, without installing 12 programs and taking a bioinformatics course?
Like, without installing 12 programs and taking a bioinformatics course?
I hope their antibodies are better than their legos. The design is very flimsy, but I enjoyed the challenge.
January 7, 2026 at 3:59 AM
I hope their antibodies are better than their legos. The design is very flimsy, but I enjoyed the challenge.
Reposted by Phil Kyriakakis
Surprise mother$#@%er!
Q: What does this mean for neural systems to understand them?
A: We absolutely need the wiring diagram. All synapses, chemical and electrical.
#Connectomics
Q: What does this mean for neural systems to understand them?
A: We absolutely need the wiring diagram. All synapses, chemical and electrical.
#Connectomics
Evidence suggests that transcriptionally similar cells can be functionally diverse
go.nature.com/3N4lT2d
go.nature.com/3N4lT2d
Does a cell’s gene expression always reflect its function?
Transcriptionally similar neurons can be functionally diverse.
go.nature.com
January 1, 2026 at 6:55 AM
Surprise mother$#@%er!
Q: What does this mean for neural systems to understand them?
A: We absolutely need the wiring diagram. All synapses, chemical and electrical.
#Connectomics
Q: What does this mean for neural systems to understand them?
A: We absolutely need the wiring diagram. All synapses, chemical and electrical.
#Connectomics
They win for best use of parafilm: www.nature.com/articles/s41...
Fluorine-free binder-based dry thick electrodes with Parafilm® M toward sustainable and efficient battery manufacturing - Nature Communications
Fluorine-containing binders in battery dry electrode processing raise environmental concerns regarding restrictions on per- and polyfluoroalkyl substances. Here, authors show that a fluorine-free bind...
www.nature.com
December 23, 2025 at 9:10 AM
They win for best use of parafilm: www.nature.com/articles/s41...
👀
🧪 This will blow your mind
“A process which led from the amoeba to man appeared to the philosophers to be obviously a progress - though whether the amoeba would agree with this opinion is not known"
- Bertrand Russell, 1976.
Time-lapse video of Vampyrella lateritia eating Spirogyra algae from Science Source/Oliver Skibbe. 🦠
- Bertrand Russell, 1976.
Time-lapse video of Vampyrella lateritia eating Spirogyra algae from Science Source/Oliver Skibbe. 🦠
December 19, 2025 at 9:55 AM
👀
How to write an R01 type grant that isn’t “too high risk”, but is still exciting, novel?
December 19, 2025 at 5:57 AM
How to write an R01 type grant that isn’t “too high risk”, but is still exciting, novel?
Reposted by Phil Kyriakakis
This is VERY cool! 🧪
🗞️ New preprint from the lab, led by our postdoc Ana Garoña (not on here) in collab with @andreagiometto.bsky.social: “Experimental evolution of cellular miniaturization reveals a mechanism for cell size evolution”, aka: “honey, we shrank the yeasts!” 🎥
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
December 15, 2025 at 4:07 PM
This is VERY cool! 🧪
Son got sick (severe cold) in Taiwan, no insurance. Saw a doctor in 10min, $15 including the acetaminophen and ibuprofen the doctor gave us. How much would that be in the USA?
December 13, 2025 at 9:50 AM
Son got sick (severe cold) in Taiwan, no insurance. Saw a doctor in 10min, $15 including the acetaminophen and ibuprofen the doctor gave us. How much would that be in the USA?
This website was meant for scientist runners! They literally cut the shoes in 1/2 and do all sorts of testing on them!
runrepeat.com/saucony-ride...
runrepeat.com/saucony-ride...
Cut in half: Saucony Ride 17 Review (2024)
Saucony Ride 17 review: The Saucony Ride 17 is more than just a minor update—it's a significant overhaul, especially with the all-new midsole that enhances energy return and responsiveness. In our lab...
runrepeat.com
December 1, 2025 at 7:00 AM
This website was meant for scientist runners! They literally cut the shoes in 1/2 and do all sorts of testing on them!
runrepeat.com/saucony-ride...
runrepeat.com/saucony-ride...
Reposted by Phil Kyriakakis
PI sharing with his student a competitor's ERC that he's reviewing
November 27, 2025 at 6:31 PM
PI sharing with his student a competitor's ERC that he's reviewing
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
November 22, 2025 at 5:10 AM
Update, I grabbed 10 colonies and combined into one @plasmidsaurus.bsky.social reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
Reposted by Phil Kyriakakis
Boolean logic-gated protein cargo release! @coledeforest.bsky.social's group previously used the molecular topology with proteases as inputs to release fluorescent proteins from hydrogels, and they now extended the platform for other protein cargoes! 🧬
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
November 20, 2025 at 12:44 PM
Boolean logic-gated protein cargo release! @coledeforest.bsky.social's group previously used the molecular topology with proteases as inputs to release fluorescent proteins from hydrogels, and they now extended the platform for other protein cargoes! 🧬
onlinelibrary.wiley.com/doi/abs/10.1...
onlinelibrary.wiley.com/doi/abs/10.1...
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
November 19, 2025 at 6:27 AM
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
AI can mess things up real fast! 😭
November 15, 2025 at 1:22 AM
AI can mess things up real fast! 😭
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
November 6, 2025 at 6:33 AM
I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked!
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
~Protocol:
1) cut out band
2) poke hold in small tube, put gel in it, put in big tube
3) Spin for 2-3 seconds
4) sequence the liquid in the bottom tube
This may not always work, but cool! @plasmidsaurus.bsky.social
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
October 29, 2025 at 7:12 AM
iGEM is not your typical scientific conference! @igemcommunity.bsky.social
“Bang-Bang optimal light control for maximum protein
production in yeast” - love this title
hal.science/hal-05323963...
production in yeast” - love this title
hal.science/hal-05323963...
hal.science
October 26, 2025 at 8:48 PM
“Bang-Bang optimal light control for maximum protein
production in yeast” - love this title
hal.science/hal-05323963...
production in yeast” - love this title
hal.science/hal-05323963...
So many beautiful pictures and I love papers that benchmark a bunch of tools.
How can we see the cells that make up a living organism? Membrane-localising tags can drive fluorescent proteins to the cell's outer membrane, making their outlines visible. But the tags don't work well in all organisms. How do you find one for your species of interest? 🧵
Check our latest preprint
Check our latest preprint
A toolkit for testing membrane-localising tags across species
Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...
www.biorxiv.org
October 24, 2025 at 3:17 AM
So many beautiful pictures and I love papers that benchmark a bunch of tools.
If you want your research to sound “basic”, call it “basic research”. - I don’t think the general public will think it is very important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
better.today
October 20, 2025 at 3:01 PM
If you want your research to sound “basic”, call it “basic research”. - I don’t think the general public will think it is very important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
I like “fundamental science” better. Today I saw “frontier science”, which sounds cooler and more important.
Reposted by Phil Kyriakakis
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
October 15, 2025 at 12:22 PM
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
Reposted by Phil Kyriakakis
A paper in Nature Communication reports on a new method to produce strong, biodegradable plastic from bamboo. The bioplastic resembles oil-based plastics in strength, shapeability, and thermal stability but can biodegrade in soil within 50 days. go.nature.com/4h1xv0X 🧪
October 15, 2025 at 1:50 AM
A paper in Nature Communication reports on a new method to produce strong, biodegradable plastic from bamboo. The bioplastic resembles oil-based plastics in strength, shapeability, and thermal stability but can biodegrade in soil within 50 days. go.nature.com/4h1xv0X 🧪