Alexandria Shaw
@alexlouiseshaw.bsky.social
PhD Candidate at UBC Biochemistry with John Burke and Calvin Yip | CIHR Doctoral Scholar | Structural Biology | Cryo-EM | HDX-MS | Kinases and Phospholipids | 🇨🇦 | Seeking a postdoctoral position
Reposted by Alexandria Shaw
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted by Alexandria Shaw
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA pubmed.ncbi.nlm.nih.gov/41197736/ #cryoem
November 7, 2025 at 7:10 PM
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA pubmed.ncbi.nlm.nih.gov/41197736/ #cryoem
Reposted by Alexandria Shaw
Sushant and Damilola in my group were running the BLI instrument nonstop to generate all of these curves. Alex was able to solve the cryo EM structure of this nanobody bound to PI4KA-TTC7B
November 5, 2025 at 10:35 PM
Sushant and Damilola in my group were running the BLI instrument nonstop to generate all of these curves. Alex was able to solve the cryo EM structure of this nanobody bound to PI4KA-TTC7B
Reposted by Alexandria Shaw
Cool new paper (www.jbc.org/article/S002... from my lab spearheaded by graduate students Sushant @sushantsuresh.bsky.social and Alex @alexlouiseshaw.bsky.social . This was a very cool collaboration with the Scott Hansen, Jennifer Cobb, Tamas Balla labs
www.jbc.org
November 5, 2025 at 10:31 PM
Cool new paper (www.jbc.org/article/S002... from my lab spearheaded by graduate students Sushant @sushantsuresh.bsky.social and Alex @alexlouiseshaw.bsky.social . This was a very cool collaboration with the Scott Hansen, Jennifer Cobb, Tamas Balla labs
Reposted by Alexandria Shaw
HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC!
We are proud to have provided the electron microscopy for this work!
Read more:
doi.org/10.1016/j.st...
We are proud to have provided the electron microscopy for this work!
Read more:
doi.org/10.1016/j.st...
Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A
Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane.…
www.sciencedirect.com
May 14, 2025 at 6:30 PM
HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC!
We are proud to have provided the electron microscopy for this work!
Read more:
doi.org/10.1016/j.st...
We are proud to have provided the electron microscopy for this work!
Read more:
doi.org/10.1016/j.st...
Reposted by Alexandria Shaw
New review out from my lab on class I PI3Ks www.sciencedirect.com/science/arti..., was fun to write where we are and where we are going in PI3K research
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social
Molecular basis for regulation of the class I phosphoinositide 3-kinases (PI3Ks), and their targeting in human disease
The class I phosphoinositide 3-kinase pathway (PI3K) is a master regulator of cellular growth, and plays essential roles in controlling immune cell fu…
www.sciencedirect.com
September 10, 2025 at 10:07 PM
New review out from my lab on class I PI3Ks www.sciencedirect.com/science/arti..., was fun to write where we are and where we are going in PI3K research
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social
Reposted by Alexandria Shaw
Really just had something like this feeling this afternoon. It’s the high that keeps you coming back for more
August 8, 2025 at 1:27 AM
Really just had something like this feeling this afternoon. It’s the high that keeps you coming back for more
Reposted by Alexandria Shaw
Great collaborative work where we developed a nanobody based tool to block EFR3 mediated recruitment of PI4KA. A potential therapeutic window maybe?
Thanks @alexlouiseshaw.bsky.social for the assist!
Thanks @alexlouiseshaw.bsky.social for the assist!
New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA https://www.biorxiv.org/content/10.1101/2025.07.28.667261v1
August 7, 2025 at 5:13 AM
Great collaborative work where we developed a nanobody based tool to block EFR3 mediated recruitment of PI4KA. A potential therapeutic window maybe?
Thanks @alexlouiseshaw.bsky.social for the assist!
Thanks @alexlouiseshaw.bsky.social for the assist!
New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA https://www.biorxiv.org/content/10.1101/2025.07.28.667261v1
August 6, 2025 at 4:19 AM
New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
New review out with @jburkevic.bsky.social!
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides
portlandpress.com/biochemsoctr...
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides
portlandpress.com/biochemsoctr...
Molecular insight on the role of the phosphoinositide PIP3 in regulating the protein kinases Akt, PDK1, and BTK
Protein kinases are master regulators of myriad processes in eukaryotic cells playing critical roles in growth, metabolism, cellular differentiation, and motility. A subclass of protein kinases is reg...
portlandpress.com
July 7, 2025 at 10:15 PM
New review out with @jburkevic.bsky.social!
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides
portlandpress.com/biochemsoctr...
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides
portlandpress.com/biochemsoctr...
Reposted by Alexandria Shaw
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is
radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
April 23, 2025 at 4:23 AM
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
🔗 www.pnas.org/doi/10.1073/...
Reposted by Alexandria Shaw
New work out from my lab by my graduate student Isobel in collaboration with Josh Pemberton and Tamas Balla. Provides some new cool tools to manipulate PI3,5P2 levels, as well as some new insight into PIKfyve regulation.
rupress.org/jcb/article-...
rupress.org/jcb/article-...
An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate
In this paper, Pemberton et al. describe the generation and characterization of an engineered active fragment of the PIKfyve enzyme and its use to generate
rupress.org
March 31, 2025 at 4:22 PM
New work out from my lab by my graduate student Isobel in collaboration with Josh Pemberton and Tamas Balla. Provides some new cool tools to manipulate PI3,5P2 levels, as well as some new insight into PIKfyve regulation.
rupress.org/jcb/article-...
rupress.org/jcb/article-...
Reposted by Alexandria Shaw
An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. New study from Joshua Pemberton, John Burke, Tamas Balla (National Institute of Child Health and Human Development) and colleagues: rupress.org/jcb/article/...
#Biochemistry #CellSignaling #lipid
#Biochemistry #CellSignaling #lipid
April 2, 2025 at 3:12 PM
An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. New study from Joshua Pemberton, John Burke, Tamas Balla (National Institute of Child Health and Human Development) and colleagues: rupress.org/jcb/article/...
#Biochemistry #CellSignaling #lipid
#Biochemistry #CellSignaling #lipid
Reposted by Alexandria Shaw
Back from vacation and really glad to share some work from my PhD research. Great collaborative effort @alexlouiseshaw.bsky.social Check out this post from my PI to learn more about Lipid kinase PI4KA and its regulation!
First post up, sharing some cool work from my lab by @alexlouiseshaw.bsky.social and @sushantsuresh.bsky.social out today in Science Advances (www.science.org/doi/10.1126/...)
Molecular basis for plasma membrane recruitment of PI4KA by EFR3
The structure of the EFR3A-PI4KA complex reveals insight into PI4KA membrane localization and its role in human disease.
www.science.org
January 6, 2025 at 5:08 PM
Back from vacation and really glad to share some work from my PhD research. Great collaborative effort @alexlouiseshaw.bsky.social Check out this post from my PI to learn more about Lipid kinase PI4KA and its regulation!
No better way to start the holidays then being able to share that some of my PhD research is live on Science Advances today!😄 A great collaborative effort! Check out this thread from my PI!
First post up, sharing some cool work from my lab by @alexlouiseshaw.bsky.social and @sushantsuresh.bsky.social out today in Science Advances (www.science.org/doi/10.1126/...)
Molecular basis for plasma membrane recruitment of PI4KA by EFR3
The structure of the EFR3A-PI4KA complex reveals insight into PI4KA membrane localization and its role in human disease.
www.science.org
December 21, 2024 at 12:01 AM
No better way to start the holidays then being able to share that some of my PhD research is live on Science Advances today!😄 A great collaborative effort! Check out this thread from my PI!