At long last, the Chlamy chloroplast ribosome hat 🎩 (now renamed the arm 💪) is here to say hello👋! We first spotted this extended structure in green algae a decade ago, and @florentwaltz.bsky.social did beautiful work bringing it into focus! #CryoEM ❄️ #TeamTomo 🔬 #PlantScience 🌾 #ProtistsOnSky 🧪
Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
February 10, 2026 at 1:53 PM
At long last, the Chlamy chloroplast ribosome hat 🎩 (now renamed the arm 💪) is here to say hello👋! We first spotted this extended structure in green algae a decade ago, and @florentwaltz.bsky.social did beautiful work bringing it into focus! #CryoEM ❄️ #TeamTomo 🔬 #PlantScience 🌾 #ProtistsOnSky 🧪
🧪❄️🔬
We found that the proteins Nono and SFPQ assemble into long filaments. Curious to know whether these filaments also exist in paraspeckles.
Very enjoyable collaboration with Dietmar Geiger at @uni-wuerzburg.de . We used #cryoEM for structure determination rdcu.be/e06t7 .
We found that the proteins Nono and SFPQ assemble into long filaments. Curious to know whether these filaments also exist in paraspeckles.
Very enjoyable collaboration with Dietmar Geiger at @uni-wuerzburg.de . We used #cryoEM for structure determination rdcu.be/e06t7 .
January 28, 2026 at 11:01 AM
🧪❄️🔬
We found that the proteins Nono and SFPQ assemble into long filaments. Curious to know whether these filaments also exist in paraspeckles.
Very enjoyable collaboration with Dietmar Geiger at @uni-wuerzburg.de . We used #cryoEM for structure determination rdcu.be/e06t7 .
We found that the proteins Nono and SFPQ assemble into long filaments. Curious to know whether these filaments also exist in paraspeckles.
Very enjoyable collaboration with Dietmar Geiger at @uni-wuerzburg.de . We used #cryoEM for structure determination rdcu.be/e06t7 .
A tailed archaeal virus 🦠 in all its glory: amazing #cryoEM ❄️🔬study by @daumlab.bsky.social and colleagues. Also, look at HFTV1 doing a backflip to infect its host…
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail https://www.biorxiv.org/content/10.1101/2024.12.09.627619v1
December 10, 2024 at 7:32 AM
A tailed archaeal virus 🦠 in all its glory: amazing #cryoEM ❄️🔬study by @daumlab.bsky.social and colleagues. Also, look at HFTV1 doing a backflip to infect its host…
🫣 this nucleosome review makes me feel both dumb and grateful. Should've known all of this, knew <30%. And had to read less than 6 pages to learn a lot! 🧬🧪
Bonus: lots of cryoEM ❄️🔬 references to dig into...
Bonus: lots of cryoEM ❄️🔬 references to dig into...
Structural diversity of noncanonical nucleosomes: Functions in chromatin pubmed.ncbi.nlm.nih.gov/40311546/ #cryoem
May 2, 2025 at 8:53 PM
🫣 this nucleosome review makes me feel both dumb and grateful. Should've known all of this, knew <30%. And had to read less than 6 pages to learn a lot! 🧬🧪
Bonus: lots of cryoEM ❄️🔬 references to dig into...
Bonus: lots of cryoEM ❄️🔬 references to dig into...
For those at #BPS2025 come to the #cryoem subgroup symposium Saturday 8:30-12:30am Room 515B. 🔬❄️There are also tickets available for the happy hour!
Can't wait to see you all at #BPS2025 for the #cryoem subgroup symposium!
8:30am-12:30pm, Sat Feb 15, Room 515B
See detailed program at the link below. We have some superb science lined up, so please join us for what I hope will be a fantastic morning of science!
8:30am-12:30pm, Sat Feb 15, Room 515B
See detailed program at the link below. We have some superb science lined up, so please join us for what I hope will be a fantastic morning of science!
www.dropbox.com
February 15, 2025 at 4:05 PM
If you care about how to use cryoEM to estimate conformational ensembles and populations of different states you should read this from @pilarcossio.bsky.social and team ❄️ 🔬
Counting particles could give wrong probabilities in Cryo-Electron Microscopy https://www.biorxiv.org/content/10.1101/2025.03.27.644168v1
March 29, 2025 at 4:01 PM
If you care about how to use cryoEM to estimate conformational ensembles and populations of different states you should read this from @pilarcossio.bsky.social and team ❄️ 🔬
Welcome @oraxdrf.bsky.social! Following him = news about the latest and greatest in #cryoEM ❄️🔬and #cryoET applications 🙂
April 22, 2025 at 3:35 PM
Welcome @oraxdrf.bsky.social! Following him = news about the latest and greatest in #cryoEM ❄️🔬and #cryoET applications 🙂
Thrilled to see another chapter of my PhD at Universität Osnabrück published at Nature Structural & Molecular Biology! Read more about how to make Ceramides and the Ping-Pong-Mechanism here - rdcu.be/dZZUF Thanks to all from the groups of Arne Moeller and Florian Fröhlich! #cryoEM 🔬❄️
November 14, 2024 at 3:36 PM
Thrilled to see another chapter of my PhD at Universität Osnabrück published at Nature Structural & Molecular Biology! Read more about how to make Ceramides and the Ping-Pong-Mechanism here - rdcu.be/dZZUF Thanks to all from the groups of Arne Moeller and Florian Fröhlich! #cryoEM 🔬❄️
Continuing this thread of important #CryoEM papers. ❄️ 🔬 📰 🧵
I finally read: Henderson R (1995) The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Quarterly Reviews of Biophysics 28: 171–193 […]
I finally read: Henderson R (1995) The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Quarterly Reviews of Biophysics 28: 171–193 […]
Original post on fediscience.org
fediscience.org
May 19, 2025 at 10:30 PM
Continuing this thread of important #CryoEM papers. ❄️ 🔬 📰 🧵
I finally read: Henderson R (1995) The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Quarterly Reviews of Biophysics 28: 171–193 […]
I finally read: Henderson R (1995) The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Quarterly Reviews of Biophysics 28: 171–193 […]
Today was the last day of a practical course on #CryoEM for PhD students that I helped teaching (first time for me, but this was the 3rd or 4th edition of the course). It was a lot of fun! ❄️ 🔬 🤓
It is an ambitious course. A few lectures about the theory, then mostly practicals all the way […]
It is an ambitious course. A few lectures about the theory, then mostly practicals all the way […]
Original post on fediscience.org
fediscience.org
June 3, 2025 at 11:51 AM
Today was the last day of a practical course on #CryoEM for PhD students that I helped teaching (first time for me, but this was the 3rd or 4th edition of the course). It was a lot of fun! ❄️ 🔬 🤓
It is an ambitious course. A few lectures about the theory, then mostly practicals all the way […]
It is an ambitious course. A few lectures about the theory, then mostly practicals all the way […]
Great. I tend to use 🧲 (NMR), ❄️🔬 (cryoEM) and ⚡ (anything with x-rays) for the experiments. Clearly protein is too broad as it tends to be used for 🥩🥜 etc—in fact that was my original reason to ask for a protein emoji
September 25, 2023 at 9:42 AM
Great. I tend to use 🧲 (NMR), ❄️🔬 (cryoEM) and ⚡ (anything with x-rays) for the experiments. Clearly protein is too broad as it tends to be used for 🥩🥜 etc—in fact that was my original reason to ask for a protein emoji
Our joined effort with the the Maric Lab @hmariclab.bsky.social @uniwuerzburg.bsky.social on how to aggregate capsids of Hepatitis B virus inside cells is now out in Elife doi.org/10.7554/eLif... . #cryoEM 🧪❄️🔬
January 24, 2025 at 3:43 PM
Our joined effort with the the Maric Lab @hmariclab.bsky.social @uniwuerzburg.bsky.social on how to aggregate capsids of Hepatitis B virus inside cells is now out in Elife doi.org/10.7554/eLif... . #cryoEM 🧪❄️🔬
May 13, 2025 at 4:27 PM
If you want to work in #cryoEM & #cryoET as a lab technician, at the heart of methods development and at one of the best places-to-be: please consider this opportunity within the next 3 weeks. ❄️🔬. Oh, it's close to the Alps, too. 🏔️ www.mpg.de/21854111/tec...
Technical Assistant / Lab Technician (m/f/d)
Technical assistant (m/f/div) / Lab technician (m/f/div) for electron microscopy in the Department of Cell and Virus Structure / CryoEM Technology to work with John Briggs and Juergen Plitzko:
We are ...
www.mpg.de
April 25, 2024 at 3:43 PM
If you want to work in #cryoEM & #cryoET as a lab technician, at the heart of methods development and at one of the best places-to-be: please consider this opportunity within the next 3 weeks. ❄️🔬. Oh, it's close to the Alps, too. 🏔️ www.mpg.de/21854111/tec...
Online today @science.org Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription
by @jonmarkert.bsky.social @lucas.farnunglab.com et al. at @harvardcellbio.bsky.social #cryoEM
#chromatin #transcription #cryoEM #Pol_II🧪🧬❄️🔬
www.science.org/doi/10.1126/...
by @jonmarkert.bsky.social @lucas.farnunglab.com et al. at @harvardcellbio.bsky.social #cryoEM
#chromatin #transcription #cryoEM #Pol_II🧪🧬❄️🔬
www.science.org/doi/10.1126/...
December 13, 2024 at 2:23 AM
Online today @science.org Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription
by @jonmarkert.bsky.social @lucas.farnunglab.com et al. at @harvardcellbio.bsky.social #cryoEM
#chromatin #transcription #cryoEM #Pol_II🧪🧬❄️🔬
www.science.org/doi/10.1126/...
by @jonmarkert.bsky.social @lucas.farnunglab.com et al. at @harvardcellbio.bsky.social #cryoEM
#chromatin #transcription #cryoEM #Pol_II🧪🧬❄️🔬
www.science.org/doi/10.1126/...
I'm going to keep appending to this thread when I catch up with reading important #CryoEM papers. ❄️ 🔬 📰 🧵
I recently read: Marques MA, Purdy MD & Yeager M (2019) CryoEM maps are full of potential. Current Opinion in Structural Biology 58: 214–223 https://doi.org10.1016/j.sbi.2019.04.006
It's […]
I recently read: Marques MA, Purdy MD & Yeager M (2019) CryoEM maps are full of potential. Current Opinion in Structural Biology 58: 214–223 https://doi.org10.1016/j.sbi.2019.04.006
It's […]
Original post on fediscience.org
fediscience.org
May 2, 2025 at 1:37 PM
I'm going to keep appending to this thread when I catch up with reading important #CryoEM papers. ❄️ 🔬 📰 🧵
I recently read: Marques MA, Purdy MD & Yeager M (2019) CryoEM maps are full of potential. Current Opinion in Structural Biology 58: 214–223 https://doi.org10.1016/j.sbi.2019.04.006
It's […]
I recently read: Marques MA, Purdy MD & Yeager M (2019) CryoEM maps are full of potential. Current Opinion in Structural Biology 58: 214–223 https://doi.org10.1016/j.sbi.2019.04.006
It's […]
What an incredibly useful tool to make the best possible use of “metadata” & workflow info in #cryoEM experiments and link that to data quality 😀. 🧪🔬❄️
EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition. https://www.biorxiv.org/content/10.1101/2024.02.12.579963v1
EMinsight: a tool to capture cryoEM microscope configuration and experimental outcomes for analysis and deposition. https://www.biorxiv.org/content/10.1101/2024.02.12.579963v1
The widespread adoption of cryoEM technologies for structural biology has pushed the discipline to n
www.biorxiv.org
February 14, 2024 at 3:17 PM
What an incredibly useful tool to make the best possible use of “metadata” & workflow info in #cryoEM experiments and link that to data quality 😀. 🧪🔬❄️
Not so! 😁 I combined the hashtags and keywords listed at the bottom all into the “#CryoEM” Feed. @bronwynalucas.bsky.social and @bengeliscious.bsky.social
Do you like it short as “#CryoEM”?
#cryoEM|cryoEM|cryo-EM|#cryoet|❄️🔬|#teamtomo|#insitu|#cryo-ET|cryoET|cryo-ET|#cryofib|cryofib|cryo-fibsem
Do you like it short as “#CryoEM”?
#cryoEM|cryoEM|cryo-EM|#cryoet|❄️🔬|#teamtomo|#insitu|#cryo-ET|cryoET|cryo-ET|#cryofib|cryofib|cryo-fibsem
October 1, 2023 at 7:49 PM
Not so! 😁 I combined the hashtags and keywords listed at the bottom all into the “#CryoEM” Feed. @bronwynalucas.bsky.social and @bengeliscious.bsky.social
Do you like it short as “#CryoEM”?
#cryoEM|cryoEM|cryo-EM|#cryoet|❄️🔬|#teamtomo|#insitu|#cryo-ET|cryoET|cryo-ET|#cryofib|cryofib|cryo-fibsem
Do you like it short as “#CryoEM”?
#cryoEM|cryoEM|cryo-EM|#cryoet|❄️🔬|#teamtomo|#insitu|#cryo-ET|cryoET|cryo-ET|#cryofib|cryofib|cryo-fibsem
Interesting to see myself age so much in one click of a slide 😅. Thanks a lot @ribo_rob for dropping by the @biozentrum to share your beautiful mitoribosome research and to tell us about your new company @cryocloud_io. Providing cloud processing for #CryoEM & #TeamTomo!❄️🔬☁️💻
November 23, 2024 at 6:01 AM
Cool a #TeamTomo feed! Thanks for setting this up! I was wondering if it might be useful to have all of #CryoEM❄️🔬 in one feed, but that’s the joy of Bluesky— you can always have more feeds!🫶 Also check out the Protein Cosmos feed 🧶🧬
@bengeliscious.bsky.social Thanks for using the #TeamTomo hashtag. That helped this post get scooped up by a #CryoET #TeamTomo Feed that I’m hoping will keep the #teamtomo hashtag going over here on Bluesky:
bsky.app/profile/did:...
bsky.app/profile/did:...
October 1, 2023 at 1:45 PM
Cool a #TeamTomo feed! Thanks for setting this up! I was wondering if it might be useful to have all of #CryoEM❄️🔬 in one feed, but that’s the joy of Bluesky— you can always have more feeds!🫶 Also check out the Protein Cosmos feed 🧶🧬
#Training 👨🏫 🧑🏫
#CryoEM ❄️ 🔬
#Science 🧬 🦠
#Education 📚
Huge shoutout to our SPoCs for making this a grand success! 👏
Marzia Miletto
Marcelo Alexandre de Farias
Omar Davulcu
#CryoEM ❄️ 🔬
#Science 🧬 🦠
#Education 📚
Huge shoutout to our SPoCs for making this a grand success! 👏
Marzia Miletto
Marcelo Alexandre de Farias
Omar Davulcu
September 23, 2025 at 9:59 PM
#Training 👨🏫 🧑🏫
#CryoEM ❄️ 🔬
#Science 🧬 🦠
#Education 📚
Huge shoutout to our SPoCs for making this a grand success! 👏
Marzia Miletto
Marcelo Alexandre de Farias
Omar Davulcu
#CryoEM ❄️ 🔬
#Science 🧬 🦠
#Education 📚
Huge shoutout to our SPoCs for making this a grand success! 👏
Marzia Miletto
Marcelo Alexandre de Farias
Omar Davulcu
11 days left to apply for this virtual cryoEM ❄️🔬 course 👇!
In Feb 2025 we will host a Virtual Wellcome - Advanced SPA Cryo-EM Course.
The course will be for PhD student, postdoc, staff scientist or young group leaders.
If you think it will benefit you go to the website for more info: astbury.leeds.ac.uk/facilities/w...
Sharing will be appreciated :-)
The course will be for PhD student, postdoc, staff scientist or young group leaders.
If you think it will benefit you go to the website for more info: astbury.leeds.ac.uk/facilities/w...
Sharing will be appreciated :-)
Advanced Single Particle Cryo-EM Course – February 2025 : The Astbury Centre for Structural Molecular Biology
Course overview The course is a part of the Wellcome Trust and MRC funded Cryo-EM Training Program that offers an intensive learning opportunity tailored to PhD students, postdocs, and early-career sc...
astbury.leeds.ac.uk
December 24, 2024 at 1:46 AM
11 days left to apply for this virtual cryoEM ❄️🔬 course 👇!
Finally, a direct electron detector for #cryoEM that is optimised for 100-200keV. Thank you Georg Ramm and colleagues from Monash University for exploring its capabilities! 🔬❄️ 🏔️👁️
Obtaining high-resolution cryo-EM structures using a common LaB6, 120-keV electron microscope equipped with a sub 200-keV optimised direct electron detector. https://www.biorxiv.org/content/10.1101/2024.05.26.595910v1
Obtaining high-resolution cryo-EM structures using a common LaB6, 120-keV electron microscope equipped with a sub 200-keV optimised direct electron detector. https://www.biorxiv.org/content/10.1101/2024.05.26.595910v1
Cryo-electron microscopy (Cryo-EM) single particle analysis (SPA) has become a major structural biol
www.biorxiv.org
May 28, 2024 at 9:13 PM
Finally, a direct electron detector for #cryoEM that is optimised for 100-200keV. Thank you Georg Ramm and colleagues from Monash University for exploring its capabilities! 🔬❄️ 🏔️👁️
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪
November 27, 2024 at 12:15 PM
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪