Highlights
• Metabolic state remodels mitoribosome structure and composition
• Remodeling tunes mitoribosome translation activity to match metabolic demands
• Mitoribosome activity signals to nucleus to regulate mitochondrial biogenesis
• Metabolic state remodels mitoribosome structure and composition
• Remodeling tunes mitoribosome translation activity to match metabolic demands
• Mitoribosome activity signals to nucleus to regulate mitochondrial biogenesis
November 6, 2025 at 6:54 PM
Highlights
• Metabolic state remodels mitoribosome structure and composition
• Remodeling tunes mitoribosome translation activity to match metabolic demands
• Mitoribosome activity signals to nucleus to regulate mitochondrial biogenesis
• Metabolic state remodels mitoribosome structure and composition
• Remodeling tunes mitoribosome translation activity to match metabolic demands
• Mitoribosome activity signals to nucleus to regulate mitochondrial biogenesis
This was coupled to a clearly perturbed mitoribosome assembly/translational profile, again from a low heteroplasmy (38%). If you're not seeing it immediately don't sweat - it took @mitogene.bsky.social ~5 years to teach me how to interpret these things
November 3, 2025 at 11:15 AM
This was coupled to a clearly perturbed mitoribosome assembly/translational profile, again from a low heteroplasmy (38%). If you're not seeing it immediately don't sweat - it took @mitogene.bsky.social ~5 years to teach me how to interpret these things
📚 On top of your reading list
🆕Review: Mitochondrial ribosome biogenesis and redox sensing
🔓https://buff.ly/4eG5haI
@brischigliaro.bsky.social
#Mitochondria #RedoxBiology #Ribosomes #Mitoribosome
🆕Review: Mitochondrial ribosome biogenesis and redox sensing
🔓https://buff.ly/4eG5haI
@brischigliaro.bsky.social
#Mitochondria #RedoxBiology #Ribosomes #Mitoribosome
September 10, 2025 at 9:58 AM
📚 On top of your reading list
🆕Review: Mitochondrial ribosome biogenesis and redox sensing
🔓https://buff.ly/4eG5haI
@brischigliaro.bsky.social
#Mitochondria #RedoxBiology #Ribosomes #Mitoribosome
🆕Review: Mitochondrial ribosome biogenesis and redox sensing
🔓https://buff.ly/4eG5haI
@brischigliaro.bsky.social
#Mitochondria #RedoxBiology #Ribosomes #Mitoribosome
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation www.biorxiv.org/content/10.1...
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation
Protein aggregation in various cellular compartments is a hallmark of proteostasis impairment linked to aging and numerous pathologies. Mitochondrial function depends on a balanced interplay of protei...
www.biorxiv.org
September 6, 2025 at 11:35 PM
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation www.biorxiv.org/content/10.1...
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation https://www.biorxiv.org/content/10.1101/2025.09.05.673478v1
September 6, 2025 at 10:30 PM
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation https://www.biorxiv.org/content/10.1101/2025.09.05.673478v1
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation https://www.biorxiv.org/content/10.1101/2025.09.05.673478v1
September 6, 2025 at 10:30 PM
Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation https://www.biorxiv.org/content/10.1101/2025.09.05.673478v1
Angelica Zamudio-Ochoa, Aldo García-Guerrero, Xochitl Pérez-Martínez & colleagues interrogate the translational activation mechanisms of the COX1 mRNA in yeast mitochondria.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess
September 5, 2025 at 9:04 AM
Angelica Zamudio-Ochoa, Aldo García-Guerrero, Xochitl Pérez-Martínez & colleagues interrogate the translational activation mechanisms of the COX1 mRNA in yeast mitochondria.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess
Article: The late stages of yeast mitoribosome large subunit biogenesis
#research supported by Knut and Alice Wallenberg Foundation
#science #biochemistry @mitolab.bsky.social
tinyurl.com/3444zu6x
#research supported by Knut and Alice Wallenberg Foundation
#science #biochemistry @mitolab.bsky.social
tinyurl.com/3444zu6x
The late stages of yeast mitoribosome large subunit biogenesis
The Saccharomyces cerevisiae mitoribosome synthesizes eight mitochondrial DNA-encoded proteins essential for oxidative phosphorylation. Mitoribosome l…
tinyurl.com
September 4, 2025 at 7:33 AM
Article: The late stages of yeast mitoribosome large subunit biogenesis
#research supported by Knut and Alice Wallenberg Foundation
#science #biochemistry @mitolab.bsky.social
tinyurl.com/3444zu6x
#research supported by Knut and Alice Wallenberg Foundation
#science #biochemistry @mitolab.bsky.social
tinyurl.com/3444zu6x
Our new study in collaboration with the labs of Toni Barrientos (Miami) and Henning Urlaub (Göttingen) on late stages of mitoribosome assembly in yeast. Cryo-EM by our former PhD student Sorbhi and Julian from scilifelab. Funding by
@kawresearch.bsky.social
authors.elsevier.com/c/1liOmc5IDu...
@kawresearch.bsky.social
authors.elsevier.com/c/1liOmc5IDu...
authors.elsevier.com
September 2, 2025 at 11:02 AM
Our new study in collaboration with the labs of Toni Barrientos (Miami) and Henning Urlaub (Göttingen) on late stages of mitoribosome assembly in yeast. Cryo-EM by our former PhD student Sorbhi and Julian from scilifelab. Funding by
@kawresearch.bsky.social
authors.elsevier.com/c/1liOmc5IDu...
@kawresearch.bsky.social
authors.elsevier.com/c/1liOmc5IDu...
Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome PubMed
Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome
The biogenesis of the mitoribosomal small subunit involves a dynamic network of assembly factors. Conserved methyltransferases Mettl15 and Mettl17 act on the solvent-exposed surface of rRNA. Binding of Mettl17 is associated with the early assembly stage, whereas Mettl15 is involved in the late stage. Here, we integrate structural data from Trypanosoma brucei with mammalian homologs and molecular dynamics simulations. We reveal how the interplay of Mettl15 and Mettl17 in intermediate steps links...
dlvr.it
August 30, 2025 at 8:34 PM
Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome PubMed
Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome pubmed.ncbi.nlm.nih.gov/40882632/
August 30, 2025 at 7:16 AM
Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome pubmed.ncbi.nlm.nih.gov/40882632/
The late stages of yeast mitoribosome large subunit biogenesis pubmed.ncbi.nlm.nih.gov/40865570/ #cryoem
August 28, 2025 at 12:56 PM
The late stages of yeast mitoribosome large subunit biogenesis pubmed.ncbi.nlm.nih.gov/40865570/ #cryoem
Thank you very much for highlighting @beatascienceart.bsky.social illustration of our study on mitoribosome evolution and for explaining the work behind it. It was a wonderful collaboration, and still my favourite cover! @molbioevol.bsky.social
Exciting to see how art and science come together
Exciting to see how art and science come together
July 14, 2025 at 7:40 PM
Thank you very much for highlighting @beatascienceart.bsky.social illustration of our study on mitoribosome evolution and for explaining the work behind it. It was a wonderful collaboration, and still my favourite cover! @molbioevol.bsky.social
Exciting to see how art and science come together
Exciting to see how art and science come together
Excited to contribute to the Mitoribosome collaborative study led by @wredenberglab.bsky.social in @natcomms.nature.com🎉 "The mitochondrial methylation potential gates mitoribosome assembly"
The assembly starts before the completion of the rRNA gene cluster processing🧵
www.nature.com/articles/s41...
The assembly starts before the completion of the rRNA gene cluster processing🧵
www.nature.com/articles/s41...
June 30, 2025 at 4:22 AM
Excited to contribute to the Mitoribosome collaborative study led by @wredenberglab.bsky.social in @natcomms.nature.com🎉 "The mitochondrial methylation potential gates mitoribosome assembly"
The assembly starts before the completion of the rRNA gene cluster processing🧵
www.nature.com/articles/s41...
The assembly starts before the completion of the rRNA gene cluster processing🧵
www.nature.com/articles/s41...
Weekly selection of papers on #mitochondrialdisease!
biomed.news/bims-mitdis/...
@biomednews.bsky.social @gavinmcstay.bsky.social @mitoscientist.bsky.social
The mitochondrial methylation potential gates mitoribosome assembly @wredenberglab.bsky.social
pmc.ncbi.nlm.nih.gov/articles/PMC...
biomed.news/bims-mitdis/...
@biomednews.bsky.social @gavinmcstay.bsky.social @mitoscientist.bsky.social
The mitochondrial methylation potential gates mitoribosome assembly @wredenberglab.bsky.social
pmc.ncbi.nlm.nih.gov/articles/PMC...
June 29, 2025 at 11:17 AM
Weekly selection of papers on #mitochondrialdisease!
biomed.news/bims-mitdis/...
@biomednews.bsky.social @gavinmcstay.bsky.social @mitoscientist.bsky.social
The mitochondrial methylation potential gates mitoribosome assembly @wredenberglab.bsky.social
pmc.ncbi.nlm.nih.gov/articles/PMC...
biomed.news/bims-mitdis/...
@biomednews.bsky.social @gavinmcstay.bsky.social @mitoscientist.bsky.social
The mitochondrial methylation potential gates mitoribosome assembly @wredenberglab.bsky.social
pmc.ncbi.nlm.nih.gov/articles/PMC...
@wredenberglab.bsky.social @ruthicglasgow.bsky.social
No SAM? Mitoribosome assembly stalls. Cryo-EM reveals methylation is essential for peptidyl center formation.
Congrats to authors 🎉
Stay tuned to hear more about mitoSAM role in different tissues
#Mitochondria #RibosomeBiogenesis
rdcu.be/etStQ
No SAM? Mitoribosome assembly stalls. Cryo-EM reveals methylation is essential for peptidyl center formation.
Congrats to authors 🎉
Stay tuned to hear more about mitoSAM role in different tissues
#Mitochondria #RibosomeBiogenesis
rdcu.be/etStQ
The mitochondrial methylation potential gates mitoribosome assembly
Nature Communications - Here, using long-read RNA sequencing, SILAC proteomics, and cryo-EM, the authors show that loss of mitochondrial methylation impairs rRNA processing and ribosome maturation,...
rdcu.be
June 28, 2025 at 12:16 PM
@wredenberglab.bsky.social @ruthicglasgow.bsky.social
No SAM? Mitoribosome assembly stalls. Cryo-EM reveals methylation is essential for peptidyl center formation.
Congrats to authors 🎉
Stay tuned to hear more about mitoSAM role in different tissues
#Mitochondria #RibosomeBiogenesis
rdcu.be/etStQ
No SAM? Mitoribosome assembly stalls. Cryo-EM reveals methylation is essential for peptidyl center formation.
Congrats to authors 🎉
Stay tuned to hear more about mitoSAM role in different tissues
#Mitochondria #RibosomeBiogenesis
rdcu.be/etStQ
Article in @natcomms.nature.com: The mitochondrial methylation potentialgates mitoribosome assembly
#research supported by Knut and Alice Wallenberg Foundations #mitochondria
tinyurl.com/mryeuf9w
#research supported by Knut and Alice Wallenberg Foundations #mitochondria
tinyurl.com/mryeuf9w
June 26, 2025 at 9:50 AM
Article in @natcomms.nature.com: The mitochondrial methylation potentialgates mitoribosome assembly
#research supported by Knut and Alice Wallenberg Foundations #mitochondria
tinyurl.com/mryeuf9w
#research supported by Knut and Alice Wallenberg Foundations #mitochondria
tinyurl.com/mryeuf9w
The mitochondrial methylation potential gates mitoribosome assembly pubmed.ncbi.nlm.nih.gov/40562754/ #cryoem
June 26, 2025 at 9:28 AM
The mitochondrial methylation potential gates mitoribosome assembly pubmed.ncbi.nlm.nih.gov/40562754/ #cryoem
Methylation is essential not only for RNA stability — we show it’s structurally required for forming the peptidyl transferase center (PTC). Without it, the mitoribosome stalls at an immature stage, unable to build its catalytic core.
June 25, 2025 at 2:17 PM
Methylation is essential not only for RNA stability — we show it’s structurally required for forming the peptidyl transferase center (PTC). Without it, the mitoribosome stalls at an immature stage, unable to build its catalytic core.
Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss
➡️ buff.ly/3h5b9Rx
#Mitochondria #MitoMonday #Mitoribosome #ModelOrganisms
➡️ buff.ly/3h5b9Rx
#Mitochondria #MitoMonday #Mitoribosome #ModelOrganisms
June 2, 2025 at 10:08 AM
Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss
➡️ buff.ly/3h5b9Rx
#Mitochondria #MitoMonday #Mitoribosome #ModelOrganisms
➡️ buff.ly/3h5b9Rx
#Mitochondria #MitoMonday #Mitoribosome #ModelOrganisms
Available now - the Feature Review "Emerging mechanisms of human #MitochondrialTranslation regulation" from Antoni Barrientos and co (@brischigliaro.bsky.social).
#Mitoribosome #ProgrammedFrameshifting #RibosomeStalling #RibosomeRecycling
Read it here: authors.elsevier.com/a/1kv-f3S6Gf...
#Mitoribosome #ProgrammedFrameshifting #RibosomeStalling #RibosomeRecycling
Read it here: authors.elsevier.com/a/1kv-f3S6Gf...
April 17, 2025 at 3:33 PM
Available now - the Feature Review "Emerging mechanisms of human #MitochondrialTranslation regulation" from Antoni Barrientos and co (@brischigliaro.bsky.social).
#Mitoribosome #ProgrammedFrameshifting #RibosomeStalling #RibosomeRecycling
Read it here: authors.elsevier.com/a/1kv-f3S6Gf...
#Mitoribosome #ProgrammedFrameshifting #RibosomeStalling #RibosomeRecycling
Read it here: authors.elsevier.com/a/1kv-f3S6Gf...
Our updated CryFold preprint shows that compared to DeepMainmast, which uses additional constraints, CryFold achieves higher accuracy, greater stability and remains significantly faster. We also tested CryFold on the mitoribosome, successfully modelling 78 out of 87 chains with >80% completeness.
March 11, 2025 at 5:28 PM
Our updated CryFold preprint shows that compared to DeepMainmast, which uses additional constraints, CryFold achieves higher accuracy, greater stability and remains significantly faster. We also tested CryFold on the mitoribosome, successfully modelling 78 out of 87 chains with >80% completeness.
Another mitoribosome structure hits the stage, this time from Danica and @fraserlab.bsky.social labs! The field is growing, expanding into antibiotics research with new groups and young talented scientists joining. Great to see fresh perspectives shaping the field! 👏
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Context-specific inhibition of mitochondrial ribosomes by phenicol and oxazolidinone antibiotics
Abstract. The antibiotics chloramphenicol (CHL) and oxazolidinones, including linezolid (LZD), are known to inhibit mitochondrial translation. This can res
academic.oup.com
February 17, 2025 at 4:12 AM
Another mitoribosome structure hits the stage, this time from Danica and @fraserlab.bsky.social labs! The field is growing, expanding into antibiotics research with new groups and young talented scientists joining. Great to see fresh perspectives shaping the field! 👏
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Key takeaway 1: Mitoribosome profiling shows that CHL and LZD inhibit human mitoribosomes in a context-dependent manner 26/n
February 16, 2025 at 2:25 PM
Key takeaway 1: Mitoribosome profiling shows that CHL and LZD inhibit human mitoribosomes in a context-dependent manner 26/n
The conformational difference can in part be attributed to increased solvation in the PTC that we observed in our LZD-mitoribosome complex. 24/n
February 16, 2025 at 2:25 PM
The conformational difference can in part be attributed to increased solvation in the PTC that we observed in our LZD-mitoribosome complex. 24/n