Wilfried Rossoll
@rossolllab.bsky.social
Translational Neuroproteomics Lab | Understanding mechanisms of protein (dis)aggregation in neurodegenerative diseases | Views = own
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Wilfried Rossoll
@rossolllab.bsky.social
· Mar 13
Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies - Molecular Neurodegeneration
Background Tauopathies represent a diverse group of neurodegenerative disorders characterized by the abnormal aggregation of the microtubule-associated protein tau. Despite extensive research, the mec...
molecularneurodegeneration.biomedcentral.com
Our proximity proteomics study introducing Probe‑dependent Proximity Profiling (ProPPr) and applying it for profiling tau pathology‑associated proteins in tauopathies + @drneurochic.bsky.social & others is out now! @cziscience.bsky.social
molecularneurodegeneration.biomedcentral.com/articles/10....
molecularneurodegeneration.biomedcentral.com/articles/10....
Reposted by Wilfried Rossoll
Direct interaction between TDP-43 and Tau promotes their co-condensation, while suppressing Tau fibril formation and seeding
@dormannlab.bsky.social
www.embopress.org/doi/full/10....
@dormannlab.bsky.social
www.embopress.org/doi/full/10....
October 20, 2025 at 11:40 AM
Direct interaction between TDP-43 and Tau promotes their co-condensation, while suppressing Tau fibril formation and seeding
@dormannlab.bsky.social
www.embopress.org/doi/full/10....
@dormannlab.bsky.social
www.embopress.org/doi/full/10....
Reposted by Wilfried Rossoll
We have developed an entirely new mechanism of action for gene knockdown -> trafficking of targeted mRNAs to the lysosomal, using lysosomal trafficking oligonucleotides (LyTONs)!
Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...
Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...
Engineering antisense oligonucleotides for targeted mRNA degradation through lysosomal trafficking
Antisense oligonucleotides (ASOs) can modulate gene expression at the mRNA level, providing the ability to tackle conventionally undruggable targets and usher in an era of personalized medicine. A key...
pubs.rsc.org
June 23, 2025 at 8:01 AM
We have developed an entirely new mechanism of action for gene knockdown -> trafficking of targeted mRNAs to the lysosomal, using lysosomal trafficking oligonucleotides (LyTONs)!
Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...
Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...
Reposted by Wilfried Rossoll
We are thrilled that Prof Philip Wong, Ph.D., of John Hopkins Medicine will be our next webinar speaker!
"An AAV gene therapy for #ALS-#FTD guided by a presymptomatic #biomarker"
May 29, 2025
1:30-2:30 PM (ET)
Host: @rossolllab.bsky.social
Register: bit.ly/4jRnCDa
"An AAV gene therapy for #ALS-#FTD guided by a presymptomatic #biomarker"
May 29, 2025
1:30-2:30 PM (ET)
Host: @rossolllab.bsky.social
Register: bit.ly/4jRnCDa
May 1, 2025 at 6:23 PM
We are thrilled that Prof Philip Wong, Ph.D., of John Hopkins Medicine will be our next webinar speaker!
"An AAV gene therapy for #ALS-#FTD guided by a presymptomatic #biomarker"
May 29, 2025
1:30-2:30 PM (ET)
Host: @rossolllab.bsky.social
Register: bit.ly/4jRnCDa
"An AAV gene therapy for #ALS-#FTD guided by a presymptomatic #biomarker"
May 29, 2025
1:30-2:30 PM (ET)
Host: @rossolllab.bsky.social
Register: bit.ly/4jRnCDa
Reposted by Wilfried Rossoll
Context-dependent Interactors Regulate TDP-43 Dysfunction in ALS/FTLD
TDP-43 APEX proximity labeling to identify interactors for cytoplasmic (ΔNLS) and RNA-binding deficient (5FL) TDP variants!
By Longxin Xie and @donnellylab.bsky.social.
www.biorxiv.org/content/10.1...
TDP-43 APEX proximity labeling to identify interactors for cytoplasmic (ΔNLS) and RNA-binding deficient (5FL) TDP variants!
By Longxin Xie and @donnellylab.bsky.social.
www.biorxiv.org/content/10.1...
April 12, 2025 at 1:25 PM
Context-dependent Interactors Regulate TDP-43 Dysfunction in ALS/FTLD
TDP-43 APEX proximity labeling to identify interactors for cytoplasmic (ΔNLS) and RNA-binding deficient (5FL) TDP variants!
By Longxin Xie and @donnellylab.bsky.social.
www.biorxiv.org/content/10.1...
TDP-43 APEX proximity labeling to identify interactors for cytoplasmic (ΔNLS) and RNA-binding deficient (5FL) TDP variants!
By Longxin Xie and @donnellylab.bsky.social.
www.biorxiv.org/content/10.1...
Reposted by Wilfried Rossoll
I was thrilled for the opportunity to present today #ADPD2025 the work @PrudencioLab, in the wonderful city of Vienna where I was lucky to get an awesome tour by Dr Wilfried Rossoll! @rossolllab.bsky.social
April 5, 2025 at 11:09 AM
I was thrilled for the opportunity to present today #ADPD2025 the work @PrudencioLab, in the wonderful city of Vienna where I was lucky to get an awesome tour by Dr Wilfried Rossoll! @rossolllab.bsky.social
Reposted by Wilfried Rossoll
Join Dr Grinberg’s team at Mayo Clinic exploring sleep dysfunction in neurodegenerative diseases, with advanced training and exceptional career prospects. Closing date: 25th May
www.dementiaresearcher.nihr.ac.uk/job/research...
www.dementiaresearcher.nihr.ac.uk/job/research...
Research Fellow: Neuroscience - DEMENTIA RESEARCHER
Join Dr Grinberg’s team at Mayo Clinic exploring sleep dysfunction in neurodegenerative diseases, with advanced training and exceptional career prospects.
www.dementiaresearcher.nihr.ac.uk
March 30, 2025 at 6:21 PM
Join Dr Grinberg’s team at Mayo Clinic exploring sleep dysfunction in neurodegenerative diseases, with advanced training and exceptional career prospects. Closing date: 25th May
www.dementiaresearcher.nihr.ac.uk/job/research...
www.dementiaresearcher.nihr.ac.uk/job/research...
Our proximity proteomics study introducing Probe‑dependent Proximity Profiling (ProPPr) and applying it for profiling tau pathology‑associated proteins in tauopathies + @drneurochic.bsky.social & others is out now! @cziscience.bsky.social
molecularneurodegeneration.biomedcentral.com/articles/10....
molecularneurodegeneration.biomedcentral.com/articles/10....
Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies - Molecular Neurodegeneration
Background Tauopathies represent a diverse group of neurodegenerative disorders characterized by the abnormal aggregation of the microtubule-associated protein tau. Despite extensive research, the mec...
molecularneurodegeneration.biomedcentral.com
March 13, 2025 at 10:56 PM
Our proximity proteomics study introducing Probe‑dependent Proximity Profiling (ProPPr) and applying it for profiling tau pathology‑associated proteins in tauopathies + @drneurochic.bsky.social & others is out now! @cziscience.bsky.social
molecularneurodegeneration.biomedcentral.com/articles/10....
molecularneurodegeneration.biomedcentral.com/articles/10....
Reposted by Wilfried Rossoll
“In neurons, RiboExM revealed a functional dichotomy between monosomes and polysomes. Monosomes were enriched in distal neurites at baseline, whereas polysomes formed dynamically in response to external stimuli…”
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
A subcellular map of translational machinery composition and regulation at the single-molecule level
Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. In this study, we present ribosome expansion micros...
www.science.org
March 7, 2025 at 7:59 PM
“In neurons, RiboExM revealed a functional dichotomy between monosomes and polysomes. Monosomes were enriched in distal neurites at baseline, whereas polysomes formed dynamically in response to external stimuli…”
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Wilfried Rossoll
A new understanding of tissue biology from MS-based proteomics at single-cell resolution www.nature.com/artic...
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#proteomics #prot-paper
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#proteomics #prot-paper
December 6, 2024 at 7:20 PM
A new understanding of tissue biology from MS-based proteomics at single-cell resolution www.nature.com/artic...
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#proteomics #prot-paper
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#proteomics #prot-paper
Reposted by Wilfried Rossoll
The nano splits paper is out! Single cell seq and single cell proteomics on the same single (one) normal sized human cell! proteomicsnews.blogspot.com/2024/12/the-...
THE (real) single cell proteomics technique scSeq people love - NanoSplits- is out!
Check out one of my favorite techniques of the last few years - the NanoSplits paper here! The first preprint of this study is somewhere...
proteomicsnews.blogspot.com
December 6, 2024 at 11:51 AM
The nano splits paper is out! Single cell seq and single cell proteomics on the same single (one) normal sized human cell! proteomicsnews.blogspot.com/2024/12/the-...
Reposted by Wilfried Rossoll
Final episode of “what is a proteomics?” on #THEProteomicsShow with me and @proteomicsnews.bsky.social. This week we had Dorte Bekker-Jensen on talking about who can do proteomics. Obviously anyone, right?! Get it wherever you get fine podcasts.
November 29, 2024 at 1:58 PM
Final episode of “what is a proteomics?” on #THEProteomicsShow with me and @proteomicsnews.bsky.social. This week we had Dorte Bekker-Jensen on talking about who can do proteomics. Obviously anyone, right?! Get it wherever you get fine podcasts.
Reposted by Wilfried Rossoll
Benjamin Ryskeldi-Falcon’s @brfalcon.bsky.social group in the LMB’s Neurobiology Division, with Karen Duff's group at the UK DRI, have discovered that tau filaments are tethered to the membranes of extracellular vesicles in #Alzheimers
Read more: www2.mrc-lmb.cam.ac.uk/cryo-et-reve...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/cryo-et-reve...
#LMBResearch
November 26, 2024 at 3:54 PM
Benjamin Ryskeldi-Falcon’s @brfalcon.bsky.social group in the LMB’s Neurobiology Division, with Karen Duff's group at the UK DRI, have discovered that tau filaments are tethered to the membranes of extracellular vesicles in #Alzheimers
Read more: www2.mrc-lmb.cam.ac.uk/cryo-et-reve...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/cryo-et-reve...
#LMBResearch
Reposted by Wilfried Rossoll
Reposted by Wilfried Rossoll
An impressive display of technologies was used to discover how cryptic #splicing causes #celldeath and leads to development of human #diseases.
www.science.org/doi/10.1126/...
It was a real joy to work together with @prieto-garcia.bsky.social and all other collaborators. Many thx 👍😀
www.science.org/doi/10.1126/...
It was a real joy to work together with @prieto-garcia.bsky.social and all other collaborators. Many thx 👍😀
November 17, 2024 at 10:42 PM
An impressive display of technologies was used to discover how cryptic #splicing causes #celldeath and leads to development of human #diseases.
www.science.org/doi/10.1126/...
It was a real joy to work together with @prieto-garcia.bsky.social and all other collaborators. Many thx 👍😀
www.science.org/doi/10.1126/...
It was a real joy to work together with @prieto-garcia.bsky.social and all other collaborators. Many thx 👍😀
Looks like PPS Silent Surfactant, Progenta anionic acid labile surfactant (AALS) I, AALS II, cationic acid labile surfactant (CALS) I, and CALS II are not commercially available anymore
Can someone please point us to a source for mass spectrometry compatible surfactants less pricey than Invitrosol, ProteaseMax, and RapiGest SF? DDM did not work that well in our hands for FFPE tissue extraction and proteomics.
November 21, 2024 at 2:53 PM
Looks like PPS Silent Surfactant, Progenta anionic acid labile surfactant (AALS) I, AALS II, cationic acid labile surfactant (CALS) I, and CALS II are not commercially available anymore
Can someone please point us to a source for mass spectrometry compatible surfactants less pricey than Invitrosol, ProteaseMax, and RapiGest SF? DDM did not work that well in our hands for FFPE tissue extraction and proteomics.
November 21, 2024 at 2:53 PM
Can someone please point us to a source for mass spectrometry compatible surfactants less pricey than Invitrosol, ProteaseMax, and RapiGest SF? DDM did not work that well in our hands for FFPE tissue extraction and proteomics.
2nd biennial conference on TDP-43 function and dysfunction in disease"
9-11 September 2025 | Trieste, Italy www.icgeb.org/tdp43-confer...
9-11 September 2025 | Trieste, Italy www.icgeb.org/tdp43-confer...
Arturo Falaschi Conference “2nd biennial conference on TDP-43 function and dysfunction in disease”
Trieste, Italy 9 – 11 September 2025
www.icgeb.org
November 19, 2024 at 2:03 PM
2nd biennial conference on TDP-43 function and dysfunction in disease"
9-11 September 2025 | Trieste, Italy www.icgeb.org/tdp43-confer...
9-11 September 2025 | Trieste, Italy www.icgeb.org/tdp43-confer...
Reposted by Wilfried Rossoll
Download our database of 492 (!) funding opportunities for EARLY-CAREER FACULTY AND RESEARCHERS.
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download freely here: research.jhu.edu/rdt/funding-...
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download freely here: research.jhu.edu/rdt/funding-...
November 17, 2024 at 4:38 PM
Download our database of 492 (!) funding opportunities for EARLY-CAREER FACULTY AND RESEARCHERS.
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download freely here: research.jhu.edu/rdt/funding-...
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download freely here: research.jhu.edu/rdt/funding-...
Reposted by Wilfried Rossoll
News in Proteomics Research | Astral vs TIMSTOF Ultra on real single cells? Just about evenly unreal numbers! proteomicsnews.blogs...
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#proteomics #prot-other
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#proteomics #prot-other
November 17, 2024 at 10:00 PM
News in Proteomics Research | Astral vs TIMSTOF Ultra on real single cells? Just about evenly unreal numbers! proteomicsnews.blogs...
---
#proteomics #prot-other
---
#proteomics #prot-other
Reposted by Wilfried Rossoll
Download our database of 313 different postdoctoral fellowships.
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download our database here: research.jhu.edu/rdt/funding-...
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download our database here: research.jhu.edu/rdt/funding-...
November 16, 2024 at 7:05 PM
Download our database of 313 different postdoctoral fellowships.
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download our database here: research.jhu.edu/rdt/funding-...
For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).
Download our database here: research.jhu.edu/rdt/funding-...
Reposted by Wilfried Rossoll
FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5 https://www.biorxiv.org/content/10.1101/2024.09.18.613669v1
FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5 https://www.biorxiv.org/content/10.1101/2024.09.18.613669v1
FUS is an RNA binding protein mutated in amyotrophic lateral sclerosis (ALS), a neurodegenerative di
www.biorxiv.org
September 19, 2024 at 11:15 AM
FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5 https://www.biorxiv.org/content/10.1101/2024.09.18.613669v1
Reposted by Wilfried Rossoll
Tau filaments with the Alzheimer fold in cases with MAPT mutations V337M and R406W https://www.biorxiv.org/content/10.1101/2024.04.29.591661v1
Tau filaments with the Alzheimer fold in cases with MAPT mutations V337M and R406W https://www.biorxiv.org/content/10.1101/2024.04.29.591661v1
Frontotemporal dementia (FTD) and Alzheimer's disease are the most common forms of early-onset demen
www.biorxiv.org
May 1, 2024 at 5:52 AM
Tau filaments with the Alzheimer fold in cases with MAPT mutations V337M and R406W https://www.biorxiv.org/content/10.1101/2024.04.29.591661v1
Reposted by Wilfried Rossoll
Interesting MAPT model x w/ APP KI
Single MAPT knock-in mouse models of frontotemporal dementia for sharing with the neurodegenerative research community https://www.biorxiv.org/content/10.1101/2024.11.15.623736v1
Single MAPT knock-in mouse models of frontotemporal dementia for sharing with the neurodegenerative research community https://www.biorxiv.org/content/10.1101/2024.11.15.623736v1
We recently reported development of human MAPT knock-in mice that carry single or double pathogenic
www.biorxiv.org
November 15, 2024 at 11:37 PM
Interesting MAPT model x w/ APP KI
Reposted by Wilfried Rossoll
Exhilarated to share the first original study from my lab published online @ImmunityCP. A great team endeavor spearheaded by the fantastic Sofia P. das Neves 😁
“Meningeal lymphatic function promotes oligodendrocyte survival and brain myelination”
www.cell.com/immunity/ful...
“Meningeal lymphatic function promotes oligodendrocyte survival and brain myelination”
www.cell.com/immunity/ful...
Meningeal lymphatic function promotes oligodendrocyte survival and brain myelination
The neurophysiological processes regulated by meningeal lymphatic function remain
unclear. das Neves et al. showed that meningeal lymphatic dysfunction leads to loss
of mature oligodendrocytes and dem...
www.cell.com
November 14, 2024 at 12:57 PM
Exhilarated to share the first original study from my lab published online @ImmunityCP. A great team endeavor spearheaded by the fantastic Sofia P. das Neves 😁
“Meningeal lymphatic function promotes oligodendrocyte survival and brain myelination”
www.cell.com/immunity/ful...
“Meningeal lymphatic function promotes oligodendrocyte survival and brain myelination”
www.cell.com/immunity/ful...
Reposted by Wilfried Rossoll
Excited to share our latest work! In it, we develop a new method for studying RNA localization via proximity labeling: OINC-seq! In contrast to other proximity-based methods, labels deposited on RNAs are read directly by sequencing without the need for biotinylation. biorxiv.org/cgi/content/...
November 13, 2024 at 4:46 PM
Excited to share our latest work! In it, we develop a new method for studying RNA localization via proximity labeling: OINC-seq! In contrast to other proximity-based methods, labels deposited on RNAs are read directly by sequencing without the need for biotinylation. biorxiv.org/cgi/content/...