I heard that they are working on an adapter to defeat the pressure sensor present in the Flex (but not the OT-2) that prevents it from working with the tips…..

Opentrons was supposed to present their adapter to make it work on the Flex at ASMS. They say it is delayed and beta-testing is only for their 96-channel pipette now. They say they are still working on a solution for the 8-channel Pipette.

Thank you!

Thank you!

Thank you!

Thank you!

Our study provides a first comprehensive overview of pTau-associated proteomes across tauopathies and uncovered several important findings. It is also a starting point for further ongoing ProPPr projects, where will put everything that we have learned during this long journey to good use.

Our further analysis demonstrated that infiltration of astrocytic plaques with FTL-positive microglia is a characteristic feature of CBD pathology. The observed complex co-localization patterns underscore the importance of using fluorescence microscopy to follow up on proximity proteomics findings.

To discover proteins with differential association with pTau lesions between AD, CBD, PiD and PSP, we performed statistical comparison of protein abundances with MiST. We found a strong pTau-association of ferritin light chain (FTL) for CBD but this did not identify cell types or pTau lesions.

We then selected a set of common or tauopathy-specific enriched proteins for further analysis with immunofluorescence and quantitative analysis. Represented in all tauopathies was the core retromer complex protein VPS35 that we found sequestered in several types of pTau lesions in all tauopathies.

We used AT8 phospho-tau (pTau) antibody as a probe to determine the composition of disease-specific pTau-associated proteomes in post-mortem frontal cortices of four major tauopathies in situ. We report 1,317 pTau associated proteins, with 409 being new, showing concordance with previous efforts.

To identify proteins that associate with tau pathology in these tauopathies, we developed an approach for in situ proximity labeling and isolation of aggregate-associated proteins using glass slides with standard 5μm thin formalin-fixed paraffin-embedded (FFPE) human brain tissue, termed ProPPr.

Here we addressed the great disease-specific morphologic and biochemical diversity of tau aggregates in Alzheimer’s disease (AD), corticobasal degeneration (CBD), Pick’s disease (PiD), and progressive supranuclear palsy (PSP), that remained to be explored with unbiased methods such as LC-MS/MS.