Rob Mettelman
@rmettelman.bsky.social
SJCRH Postdoc | Thomas Lab | T cell Immunity | Influenza | Virus Enthusiast | Incoming Asst Prof, Pitt CVR | New Dad
Reposted by Rob Mettelman
Excited to see our TIRTL-seq method published in Nature Methods www.nature.com/articles/s41...! Some updates after the revision: (1/4)
TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing - Nature Methods
TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.
www.nature.com
November 25, 2025 at 10:38 PM
Excited to see our TIRTL-seq method published in Nature Methods www.nature.com/articles/s41...! Some updates after the revision: (1/4)
Reposted by Rob Mettelman
We are #absolutely #thrilled to #present @cp-immunity.bsky.social - Deep profiling of human T cells defines compartmentalized clones and phenotypic trajectories across blood and tonsils: Immunity www.cell.com/immunity/ful...
Deep profiling of human T cells defines compartmentalized clones and phenotypic trajectories across blood and tonsils
Human T cell responses are often studied using blood, yet most T cells reside in tissues.
By comparing T cell receptor repertoires from millions of T cells from autologous
tonsils and blood, Sureshcha...
www.cell.com
November 26, 2025 at 3:47 PM
We are #absolutely #thrilled to #present @cp-immunity.bsky.social - Deep profiling of human T cells defines compartmentalized clones and phenotypic trajectories across blood and tonsils: Immunity www.cell.com/immunity/ful...
Reposted by Rob Mettelman
@natmethods.nature.com TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing
www.nature.com/articles/s41...
@pogorely.bsky.social @fredhutch.org @stjude.bsky.social @pgtimmune.bsky.social
www.nature.com/articles/s41...
@pogorely.bsky.social @fredhutch.org @stjude.bsky.social @pgtimmune.bsky.social
November 25, 2025 at 7:18 PM
@natmethods.nature.com TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing
www.nature.com/articles/s41...
@pogorely.bsky.social @fredhutch.org @stjude.bsky.social @pgtimmune.bsky.social
www.nature.com/articles/s41...
@pogorely.bsky.social @fredhutch.org @stjude.bsky.social @pgtimmune.bsky.social
Reposted by Rob Mettelman
High expression of IL-18 receptor alpha correlates with severe respiratory viral disease and defines T cells with reduced cytotoxic signatures
www.nature.com/articles/s41... @natcomms.nature.com @katherinekedz.bsky.social
@thedohertyinst.bsky.social @unimelb.bsky.social
www.nature.com/articles/s41... @natcomms.nature.com @katherinekedz.bsky.social
@thedohertyinst.bsky.social @unimelb.bsky.social
November 25, 2025 at 7:26 PM
High expression of IL-18 receptor alpha correlates with severe respiratory viral disease and defines T cells with reduced cytotoxic signatures
www.nature.com/articles/s41... @natcomms.nature.com @katherinekedz.bsky.social
@thedohertyinst.bsky.social @unimelb.bsky.social
www.nature.com/articles/s41... @natcomms.nature.com @katherinekedz.bsky.social
@thedohertyinst.bsky.social @unimelb.bsky.social
Reposted by Rob Mettelman
FLC is a rare, aggressive liver cancer lacking effective systemic therapies. Our vaccine targets the recurrent DNAJB1::PRKACA fusion, offering a universal neoantigen to drive anti-tumor immunity. www.nature.com/articles/s41...
A therapeutic peptide vaccine for fibrolamellar hepatocellular carcinoma: a phase 1 trial - Nature Medicine
In this phase 1 trial, treatment of patients with fibrolamellar hepatocellular carcinoma with a therapeutic peptide vaccine targeting the fusion kinase DNAJB1–PRKACA, which is the driver of the diseas...
www.nature.com
November 24, 2025 at 9:38 PM
FLC is a rare, aggressive liver cancer lacking effective systemic therapies. Our vaccine targets the recurrent DNAJB1::PRKACA fusion, offering a universal neoantigen to drive anti-tumor immunity. www.nature.com/articles/s41...
Reposted by Rob Mettelman
Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @markyarchoan.bsky.social & @pogorely.bsky.social www.nature.com/articles/s41...
TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing - Nature Methods
TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.
www.nature.com
November 24, 2025 at 9:38 PM
Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @markyarchoan.bsky.social & @pogorely.bsky.social www.nature.com/articles/s41...
Reposted by Rob Mettelman
After many rewarding years at @StJudeResearch surrounded by exceptional colleagues & groundbreaking science, I’m delighted to join @fredhutch to push boundaries in viral & cancer immunology. Grateful to St. Jude and looking forward to what’s next at FH! More here: www.fredhutch.org/en/news/cent...
Viral immunologist taps ‘incredible potential’ of immune system
New Fred Hutch Cancer Center viral immunologist Dr. Paul Thomas is working to turn the "incredible potential" of the immune system into real-life diagnostic and therapeutic applications. He studies ho...
www.fredhutch.org
November 7, 2025 at 9:12 PM
After many rewarding years at @StJudeResearch surrounded by exceptional colleagues & groundbreaking science, I’m delighted to join @fredhutch to push boundaries in viral & cancer immunology. Grateful to St. Jude and looking forward to what’s next at FH! More here: www.fredhutch.org/en/news/cent...
Reposted by Rob Mettelman
Multisystem inflammatory syndrome in children is a SARS-CoV-2 triggered Kawasaki disease
Like post acute infection syndrome, tihis is not new. But, we now have identified a trigger, and have more early event data.
www.nature.com/artic...
1/4
Like post acute infection syndrome, tihis is not new. But, we now have identified a trigger, and have more early event data.
www.nature.com/artic...
1/4
Multisystem inflammatory syndrome in children is a SARS-CoV-2 triggered Kawasaki disease
Pediatric Research - Multisystem inflammatory syndrome in children is a SARS-CoV-2 triggered Kawasaki disease
www.nature.com
October 28, 2025 at 4:23 PM
Multisystem inflammatory syndrome in children is a SARS-CoV-2 triggered Kawasaki disease
Like post acute infection syndrome, tihis is not new. But, we now have identified a trigger, and have more early event data.
www.nature.com/artic...
1/4
Like post acute infection syndrome, tihis is not new. But, we now have identified a trigger, and have more early event data.
www.nature.com/artic...
1/4
Reposted by Rob Mettelman
Antiviral MX1 transiently associates in the cytoplasm with neosynthesized viral ribonucleoproteins and, using dynein, reroutes them toward the microtubule organizing center where they remain sequestered
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
October 9, 2025 at 12:23 AM
Antiviral MX1 transiently associates in the cytoplasm with neosynthesized viral ribonucleoproteins and, using dynein, reroutes them toward the microtubule organizing center where they remain sequestered
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Reposted by Rob Mettelman
New study reveals pasteurized milk containing H5N1 influenza viral components poses minimal health risks and doesn’t alter influenza immunity. Regularly drinking contaminated pasteurized milk doesn’t increase flu susceptibility in model systems. ow.ly/stPC50X32eB
September 26, 2025 at 6:05 PM
New study reveals pasteurized milk containing H5N1 influenza viral components poses minimal health risks and doesn’t alter influenza immunity. Regularly drinking contaminated pasteurized milk doesn’t increase flu susceptibility in model systems. ow.ly/stPC50X32eB
Reposted by Rob Mettelman
Very pleased to publish this new review with Farah Wahbeh on the role of #EBV in #Multiplesclerosis. I learned a lot writing this, I hope others find it useful too
www.neurology.org/doi/10.1212/...
www.neurology.org/doi/10.1212/...
Epstein-Barr Virus in Multiple Sclerosis | Neurology Neuroimmunology & Neuroinflammation
Epstein-Barr virus (EBV) is a very common herpesvirus that infects more than 90% of
the general population. Epidemiologic data indicate that EBV is a requisite risk factor
for the development of multi...
www.neurology.org
September 16, 2025 at 1:51 PM
Very pleased to publish this new review with Farah Wahbeh on the role of #EBV in #Multiplesclerosis. I learned a lot writing this, I hope others find it useful too
www.neurology.org/doi/10.1212/...
www.neurology.org/doi/10.1212/...
Reposted by Rob Mettelman
We are hiring! The Dept of Microbiology and Molecular Genetics is looking for faculty at the Assistant or Associate professor level (tenure track). Please consider joining our vibrant microbiology and immunology community at the University of Pittsburgh School of medicine
August 20, 2025 at 7:41 PM
We are hiring! The Dept of Microbiology and Molecular Genetics is looking for faculty at the Assistant or Associate professor level (tenure track). Please consider joining our vibrant microbiology and immunology community at the University of Pittsburgh School of medicine
Reposted by Rob Mettelman
What's the difference between Trm and exhausted CD8 T cells? Simone Park's new work dissects this important question. These cell types are often confused, but are ontologically and mechanistically distinct with implications for immunotherapy responses. www.biorxiv.org/content/10.1...
Divergent ontogeny of Tissue Resident Memory and Tissue Resident Exhausted CD8+ T cells underlies distinct functional potential
Persistent antigen stimulation promotes differentiation of exhausted CD8+ T (TEX) cells. TEX cells are distinct from circulating memory T (TCIRCM) cells but share many features with tissue-resident me...
www.biorxiv.org
August 14, 2025 at 5:30 PM
What's the difference between Trm and exhausted CD8 T cells? Simone Park's new work dissects this important question. These cell types are often confused, but are ontologically and mechanistically distinct with implications for immunotherapy responses. www.biorxiv.org/content/10.1...
Reposted by Rob Mettelman
Just out on @cp-cell.bsky.social! Focusing on Conserved T-cell Epitope Regions (CTERs) increases the ability of T cells to recognize Coronaviruses of pandemic concern. This strategy is broadly applicable to other viral families @tertuapn.bsky.social @lji.org
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Highly conserved Betacoronavirus sequences are broadly recognized by human T cells
The COVID-19 pandemic highlighted the critical need for vaccine strategies capable of addressing emerging viral threats. Betacoronaviruses, including …
www.sciencedirect.com
August 6, 2025 at 5:38 PM
Just out on @cp-cell.bsky.social! Focusing on Conserved T-cell Epitope Regions (CTERs) increases the ability of T cells to recognize Coronaviruses of pandemic concern. This strategy is broadly applicable to other viral families @tertuapn.bsky.social @lji.org
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Rob Mettelman
Wanted to flag this interesting study led by our collaborators
@kinglabipd.bsky.social suggesting that at least in some contexts, the immunogenicity of H5 influenza vaccines can be improved by mutations that stabilize the HA protein.
@kinglabipd.bsky.social suggesting that at least in some contexts, the immunogenicity of H5 influenza vaccines can be improved by mutations that stabilize the HA protein.
🚨 New paper alert! 🚨
Here we show that stabilized influenza hemagglutinin HPAI H5 immunogens improve the quality of elicited antibody responses. Thank you to @open_phil for funding this work! bit.ly/4lgr0rM
Here we show that stabilized influenza hemagglutinin HPAI H5 immunogens improve the quality of elicited antibody responses. Thank you to @open_phil for funding this work! bit.ly/4lgr0rM
Stabilization of H5 highly pathogenic avian influenza hemagglutinin improves vaccine-elicited neutralizing antibody responses
Transmission of highly pathogenic avian influenza from H5 clade 2.3.4.4b has expanded in recent years to infect large populations of birds and mammals, heightening the risk of a human pandemic. Influe...
bit.ly
August 4, 2025 at 7:14 PM
Wanted to flag this interesting study led by our collaborators
@kinglabipd.bsky.social suggesting that at least in some contexts, the immunogenicity of H5 influenza vaccines can be improved by mutations that stabilize the HA protein.
@kinglabipd.bsky.social suggesting that at least in some contexts, the immunogenicity of H5 influenza vaccines can be improved by mutations that stabilize the HA protein.
Reposted by Rob Mettelman
Early-life human CD8+ T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscape @lucasite-lab.bsky.social
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
July 31, 2025 at 6:44 PM
Early-life human CD8+ T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscape @lucasite-lab.bsky.social
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Reposted by Rob Mettelman
Multisystem inflammatory syndrome (MISC) in children involve inflammation caused by SARS-CoV-2 proteins leaking from the gut into the bloodstream
Larazotide, a zonulin antagonist, promotes rapid recovery @laelyonker.bsky.social
www.science.org/doi/10.1126/...
Larazotide, a zonulin antagonist, promotes rapid recovery @laelyonker.bsky.social
www.science.org/doi/10.1126/...
July 31, 2025 at 2:28 AM
Multisystem inflammatory syndrome (MISC) in children involve inflammation caused by SARS-CoV-2 proteins leaking from the gut into the bloodstream
Larazotide, a zonulin antagonist, promotes rapid recovery @laelyonker.bsky.social
www.science.org/doi/10.1126/...
Larazotide, a zonulin antagonist, promotes rapid recovery @laelyonker.bsky.social
www.science.org/doi/10.1126/...
Reposted by Rob Mettelman
How much of the TCR repertoire can we make sense of? Can your TCR and GEX data be put in the context of other conditions/tissues? How many varieties of T cells are in the repertoire zoo? All these questions (& more) addressed in our latest preprint: MetaCoNGA www.biorxiv.org/content/10.1... . a 🧵
Diverse modes of T cell receptor sequence convergence define unique functional and cellular phenotypes
Single-cell techniques allow concurrent study of gene activity and T cell receptor (TCR) sequences, identifying connections between TCR structure and cell traits. Expanding on our CoNGA software, we p...
www.biorxiv.org
July 14, 2025 at 11:52 PM
How much of the TCR repertoire can we make sense of? Can your TCR and GEX data be put in the context of other conditions/tissues? How many varieties of T cells are in the repertoire zoo? All these questions (& more) addressed in our latest preprint: MetaCoNGA www.biorxiv.org/content/10.1... . a 🧵
Reposted by Rob Mettelman
Sang Woo (Daniel) Park and I are excited to share a new preprint, "Susceptible host dynamics explain pathogen resilience to perturbations" [1/8]
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
June 23, 2025 at 9:07 PM
Sang Woo (Daniel) Park and I are excited to share a new preprint, "Susceptible host dynamics explain pathogen resilience to perturbations" [1/8]
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Rob Mettelman
We have updated our database of funding opportunities for Early-Career Researchers (private foundations, federal, international)
We have 456 entries, for which we provide $ amount, deadline, eligibility criteria, etc.
Download this massive database here for free: research.jhu.edu/rdt/funding-...
We have 456 entries, for which we provide $ amount, deadline, eligibility criteria, etc.
Download this massive database here for free: research.jhu.edu/rdt/funding-...
May 7, 2025 at 4:46 PM
We have updated our database of funding opportunities for Early-Career Researchers (private foundations, federal, international)
We have 456 entries, for which we provide $ amount, deadline, eligibility criteria, etc.
Download this massive database here for free: research.jhu.edu/rdt/funding-...
We have 456 entries, for which we provide $ amount, deadline, eligibility criteria, etc.
Download this massive database here for free: research.jhu.edu/rdt/funding-...
Reposted by Rob Mettelman
Impaired Aire-dependent IFN signaling in the thymus precedes the protective autoantibodies to IFNα, say Artur Stoljar, Maksym Zarodniuk, Pärt Peterson and colleagues @unitartu: rupress.org/jem/article/...
#Autoimmunity #Tolerance
#Autoimmunity #Tolerance
May 7, 2025 at 1:42 PM
Impaired Aire-dependent IFN signaling in the thymus precedes the protective autoantibodies to IFNα, say Artur Stoljar, Maksym Zarodniuk, Pärt Peterson and colleagues @unitartu: rupress.org/jem/article/...
#Autoimmunity #Tolerance
#Autoimmunity #Tolerance
Reposted by Rob Mettelman
Join us on the topic of an aberrant immune-epithelial niche driving respiratory long COVID at the ATS online journal club. Please register below here. site.thoracic.org/events/an-ab...
American Thoracic Society | An Aberrant Immune–Epithelial Progenitor…
The American Thoracic Society is the world's leading medical society dedicated to accelerating the advancement of global respiratory health through…
site.thoracic.org
March 20, 2025 at 12:59 AM
Join us on the topic of an aberrant immune-epithelial niche driving respiratory long COVID at the ATS online journal club. Please register below here. site.thoracic.org/events/an-ab...
Reposted by Rob Mettelman
A study in Nature Microbiology finds that the FDA-approved antiviral baloxavir improves disease outcomes in mice infected with avian influenza A (H5N1) virus, compared with the current standard treatment of oseltamivir. https://go.nature.com/4iB93Dy 🧪
March 17, 2025 at 7:17 PM
A study in Nature Microbiology finds that the FDA-approved antiviral baloxavir improves disease outcomes in mice infected with avian influenza A (H5N1) virus, compared with the current standard treatment of oseltamivir. https://go.nature.com/4iB93Dy 🧪
Reposted by Rob Mettelman
Meeting with staff from Senator @slotkin.senate.gov (D-MI) office with Cherie Butts, Insha Zahoor and Mark Brimble.
You can amplify their impact to #FundNIH by sending your letter now: www.aai.org/Public-Affai....
#PPFPHillDay #SciPol #AAIadvocacy
You can amplify their impact to #FundNIH by sending your letter now: www.aai.org/Public-Affai....
#PPFPHillDay #SciPol #AAIadvocacy
March 5, 2025 at 4:53 PM
Meeting with staff from Senator @slotkin.senate.gov (D-MI) office with Cherie Butts, Insha Zahoor and Mark Brimble.
You can amplify their impact to #FundNIH by sending your letter now: www.aai.org/Public-Affai....
#PPFPHillDay #SciPol #AAIadvocacy
You can amplify their impact to #FundNIH by sending your letter now: www.aai.org/Public-Affai....
#PPFPHillDay #SciPol #AAIadvocacy
Reposted by Rob Mettelman
The entire business model of American R1 universities is not viable without restoration of federal funding, & every individual institution pretending it can solve this problem alone is also not viable. University presidents need to be speaking out, collectively & loudly about this, in DC, right now.
The University of Pittsburgh confirmed Friday that there would be no new Ph.D. offers of admission while Pitt works to understand how reduced federal aid could impact the institution.
The University of Pittsburgh pauses its Ph.D. admissions process amid research funding uncertainty
A spokesperson for the University told WESA Friday that the school has "temporarily paused additional Ph.D. offers of admission," while Pitt works to understand how proposed federal funding cuts could...
www.wesa.fm
February 22, 2025 at 5:14 AM
The entire business model of American R1 universities is not viable without restoration of federal funding, & every individual institution pretending it can solve this problem alone is also not viable. University presidents need to be speaking out, collectively & loudly about this, in DC, right now.