RNA localization and local translation are widespread phenomena that play key roles in a plethora of cellular processes ranging from embryo patterning to general cellular functions. The traditional paradigm assigns localization elements to cis-acting RNA sequences which assemble into complexes that regulate mRNA transport and translation, and the mRNA is generally transported while remaining translationally silent. However, recent evidence has shown that the nascent protein can also play an essential role in RNA localization and can enable polysomes to control their own transport and be delivered where and when they are needed. Two such examples are reviewed: translation factories and centrosomal mRNAs. Their comparison highlights the key role of cotranslational interactions in the spatiotemporal control of protein synthesis and protein fate.

The nucleolus is the largest membraneless nuclear organelle and a critical regulator of growth and stress responses, comprised of over 600 proteins involved in ribosome biogenesis. However, how the nu...

Gene position in the nuclear space plays an important role in gene regulation. This is exemplified b

The self-organized nucleolus is where the pre-ribosomal RNA (pre-rRNA) processing and pre-ribosomal

Spatially segregated rRNA processing dictates nucleolar morphology and drives outward progression of pre-ribosomal RNA through nucleolar phases.

Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Human Technopole (HT) is an interdisciplinary life science research institute, created and supported by the Italian Government, with the aim of developing innovative strategies to improve human health