Pawel Lorkiewicz
@pklork01.bsky.social
Mass Spec nerd and Associate Professor at University of Louisville
I create extensive exposomics and stable isotope resolved metabolomics workflows to link environmental exposures with health outcomes. Current focus: Phase II metabolomics
I create extensive exposomics and stable isotope resolved metabolomics workflows to link environmental exposures with health outcomes. Current focus: Phase II metabolomics
Really looking forward to reading this paper
Charting unknown metabolic reactions by mass spectrometry-resolved stable-isotope tracing metabolomics #NatCommun www.nature.com/articles/s41...
Charting unknown metabolic reactions by mass spectrometry-resolved stable-isotope tracing metabolomics - Nature Communications
IsoNet, an isotopologue similarity networking strategy based on mass spectrometry-resolved stable-isotope tracing metabolomics, enables ab initio elucidation of unknown metabolites and their reactions...
www.nature.com
June 1, 2025 at 11:29 PM
Really looking forward to reading this paper
Reposted by Pawel Lorkiewicz
HAPPY PRIDE 🏳️🌈
Under Trump, research using terms like “Trans,” “LGBTQ,” & “Gender identity” is being defunded. These terms reflect real people who deserve science that includes them.
Stand with us to protect LGBTQ+ science from political erasure.
grant-watch.us/nih-data.html
@rcmedphys.bsky.social
Under Trump, research using terms like “Trans,” “LGBTQ,” & “Gender identity” is being defunded. These terms reflect real people who deserve science that includes them.
Stand with us to protect LGBTQ+ science from political erasure.
grant-watch.us/nih-data.html
@rcmedphys.bsky.social
June 1, 2025 at 3:22 PM
HAPPY PRIDE 🏳️🌈
Under Trump, research using terms like “Trans,” “LGBTQ,” & “Gender identity” is being defunded. These terms reflect real people who deserve science that includes them.
Stand with us to protect LGBTQ+ science from political erasure.
grant-watch.us/nih-data.html
@rcmedphys.bsky.social
Under Trump, research using terms like “Trans,” “LGBTQ,” & “Gender identity” is being defunded. These terms reflect real people who deserve science that includes them.
Stand with us to protect LGBTQ+ science from political erasure.
grant-watch.us/nih-data.html
@rcmedphys.bsky.social
Really impressed to see applied side of #massspec analysis. Just learned about a forensic assay for ~290 drugs with over 800 transitions - all in a 5 minute chromatographic run. Running on Agilent Technologies 6495D triple quad system with an amazing sensitivity. Pretty impressive stuff.
#ASMS2025
#ASMS2025
June 1, 2025 at 10:12 PM
Starting my #ASMS2025 adventure with Waters users meeting. The TQ Absolute XR is definitely a highlight (we have the Absolute and we like it a lot; an increase in robustness is definitely a welcome feature).
Decent talks. Getting almost too technical at times but I've learned a lot.
#TeamMassSpec
Decent talks. Getting almost too technical at times but I've learned a lot.
#TeamMassSpec
June 1, 2025 at 7:31 PM
Starting my #ASMS2025 adventure with Waters users meeting. The TQ Absolute XR is definitely a highlight (we have the Absolute and we like it a lot; an increase in robustness is definitely a welcome feature).
Decent talks. Getting almost too technical at times but I've learned a lot.
#TeamMassSpec
Decent talks. Getting almost too technical at times but I've learned a lot.
#TeamMassSpec
#ASMS2025 is just around the corner. This year we will have two presentations (poster and oral) discussing our approach to #LC-MS profiling and analysis of Phase II metabolites in the urine. #teammassspec
May 23, 2025 at 4:25 PM
#ASMS2025 is just around the corner. This year we will have two presentations (poster and oral) discussing our approach to #LC-MS profiling and analysis of Phase II metabolites in the urine. #teammassspec
Reposted by Pawel Lorkiewicz
We stand with you in this time of loss and recovery.
Please consider donating to the Team Kentucky Storm Relief Fund to support those affected: secure.kentucky.gov/formservices...
#TeamKentucky #KentuckyStrong
Please consider donating to the Team Kentucky Storm Relief Fund to support those affected: secure.kentucky.gov/formservices...
#TeamKentucky #KentuckyStrong
May 19, 2025 at 1:37 PM
We stand with you in this time of loss and recovery.
Please consider donating to the Team Kentucky Storm Relief Fund to support those affected: secure.kentucky.gov/formservices...
#TeamKentucky #KentuckyStrong
Please consider donating to the Team Kentucky Storm Relief Fund to support those affected: secure.kentucky.gov/formservices...
#TeamKentucky #KentuckyStrong
Our latest manuscript describes an #LCMS platform profiling Phase II metabolites. We annotated 424 mercapturates, glucuronides, sulfates linked to over 200 environmental chemicals. This demonstrates ILGA's value for advancing #Exposomics
Link doi.org/10.1016/j.en...
#MassSpectrometry #Metabolomics
Link doi.org/10.1016/j.en...
#MassSpectrometry #Metabolomics
May 8, 2025 at 6:55 PM
Our latest manuscript describes an #LCMS platform profiling Phase II metabolites. We annotated 424 mercapturates, glucuronides, sulfates linked to over 200 environmental chemicals. This demonstrates ILGA's value for advancing #Exposomics
Link doi.org/10.1016/j.en...
#MassSpectrometry #Metabolomics
Link doi.org/10.1016/j.en...
#MassSpectrometry #Metabolomics
Reposted by Pawel Lorkiewicz
For my latest, I wrote about my HHS RIF tracking doc and why it felt important to cut through the fog of terror to capture the data:
Tracking the HHS April Fools’ Massacre
The Handbasket has created a document to capture the destruction.
www.thehandbasket.co
April 3, 2025 at 12:54 AM
For my latest, I wrote about my HHS RIF tracking doc and why it felt important to cut through the fog of terror to capture the data:
Reposted by Pawel Lorkiewicz
I created a Metabolomics starter pack. A list of researchers from the wonderful world of #metabolomics. If you would like to be added (or removed) just let me know. go.bsky.app/J3VPYKm
November 15, 2024 at 12:48 AM
I created a Metabolomics starter pack. A list of researchers from the wonderful world of #metabolomics. If you would like to be added (or removed) just let me know. go.bsky.app/J3VPYKm
When I began in analytical chemistry, I explored organic and inorganic compounds as matrices for MALDI imaging of lipids in the negative ion mode.
MALDI-MS and MS-based lipidomics were so new that my early presentations fell under OTHER → other.
Things have changed a lot since then.
MALDI-MS and MS-based lipidomics were so new that my early presentations fell under OTHER → other.
Things have changed a lot since then.
March 3, 2025 at 4:01 PM
When I began in analytical chemistry, I explored organic and inorganic compounds as matrices for MALDI imaging of lipids in the negative ion mode.
MALDI-MS and MS-based lipidomics were so new that my early presentations fell under OTHER → other.
Things have changed a lot since then.
MALDI-MS and MS-based lipidomics were so new that my early presentations fell under OTHER → other.
Things have changed a lot since then.
That will affect KY universities
Source tells me that in at least one state, universities that get INBRE (IDeA Networks of Biomedical Research Excellence) money have been told not to expect any of those funds to come in “for at least six months.”
That will basically lock out poorer students from grad/med school.
INBRE states:
That will basically lock out poorer students from grad/med school.
INBRE states:
February 20, 2025 at 6:42 PM
That will affect KY universities
Reposted by Pawel Lorkiewicz
NEW: NSF confirmed that they fired 168 employees today, out of their staff of ~1,500 feds.
This includes some people who'd finished their 1-year probationary periods, which were extended to 2-years last month without explanation. More to come.
This includes some people who'd finished their 1-year probationary periods, which were extended to 2-years last month without explanation. More to come.
February 18, 2025 at 4:16 PM
NEW: NSF confirmed that they fired 168 employees today, out of their staff of ~1,500 feds.
This includes some people who'd finished their 1-year probationary periods, which were extended to 2-years last month without explanation. More to come.
This includes some people who'd finished their 1-year probationary periods, which were extended to 2-years last month without explanation. More to come.
Reposted by Pawel Lorkiewicz
Taking Omega3s and VitD over three years slowed biological aging by 3-4 months when combined with exercise.
@ciehs.bsky.social @uoflenvirome.bsky.social
@ciehs.bsky.social @uoflenvirome.bsky.social
Taking omega-3 and vitamin D supplements over a three-year period slowed biological ageing by three to four months, particularly when combined with exercise.
https://go.nature.com/4hjjGdO
https://go.nature.com/4hjjGdO
Omega-3 supplements slow biological ageing
The anti-ageing effect was even greater when combined with vitamin D and exercise.
go.nature.com
February 3, 2025 at 6:52 PM
Taking Omega3s and VitD over three years slowed biological aging by 3-4 months when combined with exercise.
@ciehs.bsky.social @uoflenvirome.bsky.social
@ciehs.bsky.social @uoflenvirome.bsky.social
Reposted by Pawel Lorkiewicz
“Because they wouldn’t let me give it away”
my answer when people ask why did I not publish AMMS with a publishing house.
Free PDF at Scripps Research:
masspec.scripps.edu/about/mass-s...
Royalty-free print version at Amazon ($15.08):
a.co/d/8dEWO8a
my answer when people ask why did I not publish AMMS with a publishing house.
Free PDF at Scripps Research:
masspec.scripps.edu/about/mass-s...
Royalty-free print version at Amazon ($15.08):
a.co/d/8dEWO8a
February 3, 2025 at 12:17 AM
“Because they wouldn’t let me give it away”
my answer when people ask why did I not publish AMMS with a publishing house.
Free PDF at Scripps Research:
masspec.scripps.edu/about/mass-s...
Royalty-free print version at Amazon ($15.08):
a.co/d/8dEWO8a
my answer when people ask why did I not publish AMMS with a publishing house.
Free PDF at Scripps Research:
masspec.scripps.edu/about/mass-s...
Royalty-free print version at Amazon ($15.08):
a.co/d/8dEWO8a
Reposted by Pawel Lorkiewicz
So, *very* unconfirmed, but I've heard from inside a fed agency that there is a chance a significant chunk of gov websites, in particular healthcare and energy-related ones, might just go dark today at 5 pm because it's impossible to scrub the DEI stuff by then.
January 31, 2025 at 4:50 PM
So, *very* unconfirmed, but I've heard from inside a fed agency that there is a chance a significant chunk of gov websites, in particular healthcare and energy-related ones, might just go dark today at 5 pm because it's impossible to scrub the DEI stuff by then.
OK @uoflenvirome.bsky.social 🤩
Thanks for sharing my posts!!!
I want to see Bluesky becoming a livelier platform.
Thanks for sharing my posts!!!
I want to see Bluesky becoming a livelier platform.
January 31, 2025 at 5:11 PM
OK @uoflenvirome.bsky.social 🤩
Thanks for sharing my posts!!!
I want to see Bluesky becoming a livelier platform.
Thanks for sharing my posts!!!
I want to see Bluesky becoming a livelier platform.
Reposted by Pawel Lorkiewicz
I’ve heard from people inside NIH, EPA, the National Cancer Institute, the National Parks Service, and more. Signal info is in bio if you are at any federal agency and want to chat.
'Insulted and Confused': Inside Agencies, Federal Employees React to Mass Resignation Offer
Splinter is your home for news and opinions that challenge power in our political and economic system that's becoming more unhinged each and every day.
www.splinter.com
January 30, 2025 at 1:50 AM
I’ve heard from people inside NIH, EPA, the National Cancer Institute, the National Parks Service, and more. Signal info is in bio if you are at any federal agency and want to chat.
OK, we've seen it before—use glucuronidase & sulfatase, hydrolyze conjugates, release parent chemical (or phase I met) & measure that.
Call us crazy, but we thought "What if we just identify and measure all the conjugates instead?"..
So we did. papers.ssrn.com/sol3/papers....
#Massspec #metabolomics
Call us crazy, but we thought "What if we just identify and measure all the conjugates instead?"..
So we did. papers.ssrn.com/sol3/papers....
#Massspec #metabolomics
Mapping of Phase Ii Human Urinary Metabolome
Humans are constantly exposed to both natural-occurring and anthropogenic chemicals. Targeted mass spectrometry approaches are frequently used to measure a smal
papers.ssrn.com
January 29, 2025 at 4:12 PM
OK, we've seen it before—use glucuronidase & sulfatase, hydrolyze conjugates, release parent chemical (or phase I met) & measure that.
Call us crazy, but we thought "What if we just identify and measure all the conjugates instead?"..
So we did. papers.ssrn.com/sol3/papers....
#Massspec #metabolomics
Call us crazy, but we thought "What if we just identify and measure all the conjugates instead?"..
So we did. papers.ssrn.com/sol3/papers....
#Massspec #metabolomics
Reposted by Pawel Lorkiewicz
New #mzmine release out now! Matching millions of reference spectra against thousands of samples was never faster. Go and test it today.
Download: github.com/mzmine/mzmin...
youtu.be/AFv55sUn9Lg
Download: github.com/mzmine/mzmin...
youtu.be/AFv55sUn9Lg
New features in mzmine 4.5
YouTube video by mzio GmbH
youtu.be
January 28, 2025 at 1:11 PM
New #mzmine release out now! Matching millions of reference spectra against thousands of samples was never faster. Go and test it today.
Download: github.com/mzmine/mzmin...
youtu.be/AFv55sUn9Lg
Download: github.com/mzmine/mzmin...
youtu.be/AFv55sUn9Lg
Thank you for sharing this resource for free @siuzdak.bsky.social
AMMS 2025 book is live, the PDF just became available, and you can freely download the entire book here...
masspec.scripps.edu/about/mass-s...
Two new chapters, new sections, and refinements throughout.
The royalty-free print version of #AMMS 2025 will be available on Amazon next week.
masspec.scripps.edu/about/mass-s...
Two new chapters, new sections, and refinements throughout.
The royalty-free print version of #AMMS 2025 will be available on Amazon next week.
January 29, 2025 at 3:51 PM
Thank you for sharing this resource for free @siuzdak.bsky.social
Reposted by Pawel Lorkiewicz
My understanding is that clinical trials should not be interrupted, so hopefully the reports about canceled appointments are done... but it's hard to say. If you are a participant in a clinical trial (or at NIH clinical center) and have seen disruptions, I'd love to hear from you—Signal in bio.
The latest on what I've heard from inside NIH and NCI and elsewhere. It's not great!
'The Office Feels Like a Tomb': Even With Clarification, NIH Chaos Continues
Splinter is your home for news and opinions that challenge power in our political and economic system that's becoming more unhinged each and every day.
www.splinter.com
January 28, 2025 at 8:28 PM
My understanding is that clinical trials should not be interrupted, so hopefully the reports about canceled appointments are done... but it's hard to say. If you are a participant in a clinical trial (or at NIH clinical center) and have seen disruptions, I'd love to hear from you—Signal in bio.
Reposted by Pawel Lorkiewicz
I have heard some internal confirmation that NIH study sections are being suspended theoretically under the Federal Advisory Committee Act. Which is insane.
Okay maybe I'm missing it but I don't see much independent confirmation of this, but if it IS happening it is *wild*. It would mean they are literally trying to stop biomedical research from happening.
All NIH study sections canceled indefinitely. This will halt science and devastate research budgets in universities.
January 23, 2025 at 1:47 AM
I have heard some internal confirmation that NIH study sections are being suspended theoretically under the Federal Advisory Committee Act. Which is insane.
This looks like a very extensive and useful resource. The authors put a lot of work into this manuscript. Can't wait to read it.
🚨 New Publication Alert 🚨
Effective Visualization Strategies in Untargeted Metabolomics - a comprehensive review!
This was a real team effort and combined various disciplines!
Very #ProudPI in #CompMetabolomics 😊😎
Please find the link and read more about it here:
www.linkedin.com/posts/jjjvan...
Effective Visualization Strategies in Untargeted Metabolomics - a comprehensive review!
This was a real team effort and combined various disciplines!
Very #ProudPI in #CompMetabolomics 😊😎
Please find the link and read more about it here:
www.linkedin.com/posts/jjjvan...
Justin J.J. van der Hooft on LinkedIn: #visualization #metabolomics #visualization #metabolomics…
🚨 New Publication Alert 🚨
Oh, kom er eens kijken wat ik in mijn schoentje vind.... 👟🎅🤶(for the non-Dutchies - our Sinterklaas is around these days to…
www.linkedin.com
January 21, 2025 at 3:32 PM
This looks like a very extensive and useful resource. The authors put a lot of work into this manuscript. Can't wait to read it.
Our paper describing a dilute-and-shoot targeted LC-MS/MS method for measuring 35 urinary metabolites of #VOCs
and #tobacco alkaloids is now published in Journal of Chromatography B. The article is available here: www.sciencedirect.com/science/arti...
#MassSpecRSS #teamMassSpec #LC-MS #exposomics
and #tobacco alkaloids is now published in Journal of Chromatography B. The article is available here: www.sciencedirect.com/science/arti...
#MassSpecRSS #teamMassSpec #LC-MS #exposomics
January 20, 2025 at 7:48 PM
Our paper describing a dilute-and-shoot targeted LC-MS/MS method for measuring 35 urinary metabolites of #VOCs
and #tobacco alkaloids is now published in Journal of Chromatography B. The article is available here: www.sciencedirect.com/science/arti...
#MassSpecRSS #teamMassSpec #LC-MS #exposomics
and #tobacco alkaloids is now published in Journal of Chromatography B. The article is available here: www.sciencedirect.com/science/arti...
#MassSpecRSS #teamMassSpec #LC-MS #exposomics
Reposted by Pawel Lorkiewicz
Wildfire smoke is now the top source of harmful PM2.5, microscopic particulate matter that can penetrate into the lungs, in the western U.S.. An environmental toxicologist examines the impact: https://buff.ly/4gPXmbk (Luke Montrose, Colorado State University) 🌎 #climatesky #california #LAfires
LA fires: Long-term exposure to wildfire smoke is poorly understood − and a growing risk
Human bodies aren’t prepared for the toxic chemicals in smoke, and the effects can be harmful in the short term and longterm.
buff.ly
January 16, 2025 at 3:30 AM
Wildfire smoke is now the top source of harmful PM2.5, microscopic particulate matter that can penetrate into the lungs, in the western U.S.. An environmental toxicologist examines the impact: https://buff.ly/4gPXmbk (Luke Montrose, Colorado State University) 🌎 #climatesky #california #LAfires