Max Koppers
@maxkoppers.bsky.social
Axonal mRNA trafficking and local translation | Group leader
FGA/CNCR/VU Amsterdam | father of two
FGA/CNCR/VU Amsterdam | father of two
Reposted by Max Koppers
Secretome translation shaped by lysosomes and lunapark-marked ER junctions
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature
Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...
www.nature.com
November 5, 2025 at 6:27 PM
Secretome translation shaped by lysosomes and lunapark-marked ER junctions
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Max Koppers
Neuronal compartmentalization results in impoverished axonal mitochondria https://www.biorxiv.org/content/10.1101/2025.10.27.684882v1
October 29, 2025 at 7:15 PM
Neuronal compartmentalization results in impoverished axonal mitochondria https://www.biorxiv.org/content/10.1101/2025.10.27.684882v1
Reposted by Max Koppers
I am thrilled to share our latest story led by the incredibly talented Brooke D’Arcy and Camila Musso. We discover a rich world of local gene expression in radial glia, essential neural and glial precursors, and develop a new method for sub-cellular mRNA manipulation. www.biorxiv.org/content/10.1...
October 10, 2025 at 10:28 PM
I am thrilled to share our latest story led by the incredibly talented Brooke D’Arcy and Camila Musso. We discover a rich world of local gene expression in radial glia, essential neural and glial precursors, and develop a new method for sub-cellular mRNA manipulation. www.biorxiv.org/content/10.1...
Reposted by Max Koppers
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Max Koppers
Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...
September 14, 2025 at 6:12 PM
Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...
Reposted by Max Koppers
✨ Post-doc opportunity in axon biology, funded by #ATIP-Avenir!
👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches.
Apply at: emploi.cnrs.fr/Offres/CDD/U...
Thank you for sharing! 👋 🙏
👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches.
Apply at: emploi.cnrs.fr/Offres/CDD/U...
Thank you for sharing! 👋 🙏
Portail Emploi CNRS - Offre d'emploi - Post-doctoral researcher in axon biology (M/F)
emploi.cnrs.fr
September 11, 2025 at 12:24 PM
✨ Post-doc opportunity in axon biology, funded by #ATIP-Avenir!
👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches.
Apply at: emploi.cnrs.fr/Offres/CDD/U...
Thank you for sharing! 👋 🙏
👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches.
Apply at: emploi.cnrs.fr/Offres/CDD/U...
Thank you for sharing! 👋 🙏
Reposted by Max Koppers
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
September 10, 2025 at 7:18 AM
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
Reposted by Max Koppers
I'm thrilled to share our work from @weissmanlab.bsky.social (www.sciencedirect.com/science/arti...). We developed LOCL-TL, an optogenetic approach for monitoring localized translation in mammalian cells. LOCL-TL revealed two distinct strategies for mitochondrially localized translation.
August 27, 2025 at 9:44 PM
I'm thrilled to share our work from @weissmanlab.bsky.social (www.sciencedirect.com/science/arti...). We developed LOCL-TL, an optogenetic approach for monitoring localized translation in mammalian cells. LOCL-TL revealed two distinct strategies for mitochondrially localized translation.
Reposted by Max Koppers
Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization https://www.biorxiv.org/content/10.1101/2025.08.02.668268v1
August 2, 2025 at 2:30 PM
Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization https://www.biorxiv.org/content/10.1101/2025.08.02.668268v1
Reposted by Max Koppers
If you’ve ever wondered how cells tune positioning of organelles in response to cell shape change, and what happens when this goes wrong, please check out our new manuscript – mRNA trafficking controls cell-size-scaled organelle dynamics! www.nature.com/articles/s41...
mRNA trafficking directs cell-size-scaling of mitochondria distribution and function - Nature Communications
How mitochondria positioning dynamically adapts to changes in cell shape is poorly understood. Here, Bradbury et al. show that cell-size-dependent trafficking of TRAK2 mRNA underpins the cell-size-sca...
www.nature.com
August 1, 2025 at 6:30 AM
If you’ve ever wondered how cells tune positioning of organelles in response to cell shape change, and what happens when this goes wrong, please check out our new manuscript – mRNA trafficking controls cell-size-scaled organelle dynamics! www.nature.com/articles/s41...
Reposted by Max Koppers
RNA can control cell migration by acting at focal adhesions www.lifescienceeditors.com/2025/06/24/r...
RNA can control cell migration by acting at focal adhesions - Scientific editing and writing experts - Life Science Editors
Scientific editing and writing experts for manuscripts and grants
www.lifescienceeditors.com
July 26, 2025 at 8:09 PM
RNA can control cell migration by acting at focal adhesions www.lifescienceeditors.com/2025/06/24/r...
Reposted by Max Koppers
📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...
July 23, 2025 at 10:10 AM
📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...
Reposted by Max Koppers
Online Now: FMRP drives mRNP targets into translationally silenced complexes Online now:
FMRP drives mRNP targets into translationally silenced complexes
Translational repression by FMRP has been attributed primarily to ribosome stalling. We show that FMRP sequesters its target mRNAs from translation initiation. FMRP binds not only to the transcript body but also directly to cap-bound eIF4E and poly(A)-tail-bound PABPC1. Through binding these two proteins, FMRP protects its target mRNAs from translation and decay.
dlvr.it
July 10, 2025 at 3:20 PM
Online Now: FMRP drives mRNP targets into translationally silenced complexes Online now:
Reposted by Max Koppers
Kicking off the lab BlueSky with this picture of a happy hairy growing #axon!
Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨
Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨
July 12, 2025 at 10:05 AM
Kicking off the lab BlueSky with this picture of a happy hairy growing #axon!
Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨
Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨
Reposted by Max Koppers
Optogenetic storage and release of protein and mRNA in live cells and animals | Nature Communications share.google/gz8nYXZ3hUVh...
#spatialbiology #imagingtools #fluorescencemicroscopy
#spatialbiology #imagingtools #fluorescencemicroscopy
Optogenetic storage and release of protein and mRNA in live cells and animals - Nature Communications
Spatiotemporal control of biomolecules is a key challenge in cell biology. Here, authors introduce RELISR, an optogenetic condensate system enabling reversible, light-dependent sequestration and relea...
share.google
July 8, 2025 at 7:13 PM
Optogenetic storage and release of protein and mRNA in live cells and animals | Nature Communications share.google/gz8nYXZ3hUVh...
#spatialbiology #imagingtools #fluorescencemicroscopy
#spatialbiology #imagingtools #fluorescencemicroscopy
Reposted by Max Koppers
Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit!
tinyurl.com/ydn6e3ac
tinyurl.com/ydn6e3ac
June 30, 2025 at 9:15 PM
Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit!
tinyurl.com/ydn6e3ac
tinyurl.com/ydn6e3ac
Reposted by Max Koppers
Researchers have found that turning down a single gene, EMC10, can boost memory and brain function in mice with a genetic condition linked to #schizophrenia. The findings offer hope for future treatments for 22q11.2 deletion syndrome.
Targeting 22q11.2 deletion syndrome
Gene-targeting molecules known as antisense oligonucleotides help improve cognitive function in mouse models of a severe chromosomal disorder.
buff.ly
June 4, 2025 at 10:03 AM
Researchers have found that turning down a single gene, EMC10, can boost memory and brain function in mice with a genetic condition linked to #schizophrenia. The findings offer hope for future treatments for 22q11.2 deletion syndrome.
Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators
Activated Slack potassium channels bind translation regulators to stimulate translation of cortical β-actin mRNA.
www.science.org
June 1, 2025 at 8:22 AM
Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Programmable control of spatial transcriptome in live cells and neurons.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Programmable control of spatial transcriptome in live cells and neurons - Nature
CRISPR-TO, a system for programmable control of spatial localization of cellular RNAs, is presented and enables functional investigation of endogenous RNA localization in diverse living cells.
www.nature.com
May 22, 2025 at 2:55 PM
Programmable control of spatial transcriptome in live cells and neurons.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Max Koppers
A 🧵 to walk those interested through this paper...
Online now at @cp-cell.bsky.social , the culmination of a 12-year effort. 🧵 to follow later this evening
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Repeat-element RNAs integrate a neuronal growth circuit
SINEs of recovery: A subset of small RNAs encoded by repetitive genomic elements are
induced by nerve lesion to regulate protein synthesis and enhance axonal growth.
www.cell.com
May 16, 2025 at 4:50 PM
A 🧵 to walk those interested through this paper...
Reposted by Max Koppers
Murphy et al. @amsterdamumc.bsky.social show that MUNC13 is essential for dense core vesicle secretion, while RIM prevents MUNC13 degradation. Key membrane-binding domains of RIM & MUNC13 play differing roles, revealing distinct mechanisms from synaptic vesicle release. rupress.org/jcb/article/...
May 12, 2025 at 4:19 PM
Murphy et al. @amsterdamumc.bsky.social show that MUNC13 is essential for dense core vesicle secretion, while RIM prevents MUNC13 degradation. Key membrane-binding domains of RIM & MUNC13 play differing roles, revealing distinct mechanisms from synaptic vesicle release. rupress.org/jcb/article/...
Reposted by Max Koppers
What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity, revealing impact of encoded protein function. Fantastic work from @lindsayabecker.bsky.social @sofiquinodoz.bsky.social @davidaknowles.bsky.social www.biorxiv.org/content/10.1...
Genome-wide mapping of mesoscale neuronal RNA organization and condensation
Subcellular RNA organization can affect critical cellular functions. However, our understanding of RNA microenvironments, particularly biomolecular condensates, remains limited, largely due to a lack ...
www.biorxiv.org
April 21, 2025 at 1:48 PM
What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity, revealing impact of encoded protein function. Fantastic work from @lindsayabecker.bsky.social @sofiquinodoz.bsky.social @davidaknowles.bsky.social www.biorxiv.org/content/10.1...
Reposted by Max Koppers
Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...
Nanobinders for Synaptotagmin 1 enable the analysis of synaptic vesicle dynamics in rodent and human models.
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes to be affected in neural diseases. Monitoring this process, and in particular synaptic vesicle recycl...
www.biorxiv.org
April 22, 2025 at 9:27 AM
Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...
Reposted by Max Koppers
In getting too excited about the front cover, I forgot to link to the actual paper 🤦♂️ Here it is: Dynamic modulation of the motor neuron translatome during developmental synapse elimination | Science Signaling www.science.org/doi/10.1126/...
Dynamic modulation of the motor neuron translatome during developmental synapse elimination
Superfluous axons are removed from muscle fibers through temporal changes in mRNA translation.
www.science.org
April 15, 2025 at 6:24 PM
In getting too excited about the front cover, I forgot to link to the actual paper 🤦♂️ Here it is: Dynamic modulation of the motor neuron translatome during developmental synapse elimination | Science Signaling www.science.org/doi/10.1126/...