Dave Shechner
@shechnerlab.bsky.social
The Shechner lab in UW Pharmacology. We study Noncoding RNAs and cellular architecture, and we build “democratized” RNA-focused chemical biology and genomics tools.
He/His/Him. Almost cartainly not D.B. Cooper
ShechnerLab.org
He/His/Him. Almost cartainly not D.B. Cooper
ShechnerLab.org
Pinned
Dave Shechner
@shechnerlab.bsky.social
· Nov 21
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
AWESOME CONFERENCE ALERT!!
I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun!
Talk abstract deadline: 10/17
Details👇
I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun!
Talk abstract deadline: 10/17
Details👇
September 19, 2025 at 11:44 PM
AWESOME CONFERENCE ALERT!!
I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun!
Talk abstract deadline: 10/17
Details👇
I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun!
Talk abstract deadline: 10/17
Details👇
I’m a bit late to the game here, but wanted to highlight this very clever technique from @paraspeckle.bsky.social’s group! Congrats to all authors!!
New method drop:
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
August 2, 2025 at 6:55 PM
I’m a bit late to the game here, but wanted to highlight this very clever technique from @paraspeckle.bsky.social’s group! Congrats to all authors!!
A day late but STILL IMPORTANT
August 2, 2025 at 4:14 PM
A day late but STILL IMPORTANT
Woohoo! So psyched to see this awesome work from the Jovanovic lab out in the world! Congrats to all the authors on this tour-de-force!
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stress
SPIDR, a massively multiplexed method that simultaneously maps dozens of RNA-binding
proteins to their RNA targets at single-nucleotide resolution, uncovers new RNA-protein
interactions and provides c...
www.cell.com
July 23, 2025 at 5:30 AM
Woohoo! So psyched to see this awesome work from the Jovanovic lab out in the world! Congrats to all the authors on this tour-de-force!
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit!
tinyurl.com/ydn6e3ac
tinyurl.com/ydn6e3ac
June 30, 2025 at 9:15 PM
Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit!
tinyurl.com/ydn6e3ac
tinyurl.com/ydn6e3ac
TOP FIVE METABOLIC PATHWAYS
…Based on whether they sound like the name of an old-school anime:
5. The Pentose-Phosphate Shunt
4. Peptidoglycan Degradation
3. Fatty Acid Beta-Oxidation
2. The Dark Reactions of the Calvin Cycle
1. Gluconeogenesis
…Based on whether they sound like the name of an old-school anime:
5. The Pentose-Phosphate Shunt
4. Peptidoglycan Degradation
3. Fatty Acid Beta-Oxidation
2. The Dark Reactions of the Calvin Cycle
1. Gluconeogenesis
December 31, 2024 at 6:38 PM
TOP FIVE METABOLIC PATHWAYS
…Based on whether they sound like the name of an old-school anime:
5. The Pentose-Phosphate Shunt
4. Peptidoglycan Degradation
3. Fatty Acid Beta-Oxidation
2. The Dark Reactions of the Calvin Cycle
1. Gluconeogenesis
…Based on whether they sound like the name of an old-school anime:
5. The Pentose-Phosphate Shunt
4. Peptidoglycan Degradation
3. Fatty Acid Beta-Oxidation
2. The Dark Reactions of the Calvin Cycle
1. Gluconeogenesis
Such awesome work from @nandangokhale.bsky.social, @ramlabuw.bsky.social, and colleagues! Fantastic seeing this paradigm-changing study out in the wild. Congrats, everyone!
Our work on cellular RNA interactions with MAVS that promote antiviral signaling is finally live. So grateful to our indispensable collaborators as well as reviewers who helped to strengthen our work. #RNA #innateimmunity #virology
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
Cellular RNA interacts with MAVS to promote antiviral signaling
Antiviral signaling downstream of RIG-I–like receptors (RLRs) proceeds through a multi-protein complex organized around the adaptor protein mitochondrial antiviral signaling protein (MAVS). Protein co...
www.science.org
December 20, 2024 at 10:22 PM
Such awesome work from @nandangokhale.bsky.social, @ramlabuw.bsky.social, and colleagues! Fantastic seeing this paradigm-changing study out in the wild. Congrats, everyone!
Aw, yeah! It’s en-dash time, people!
December 17, 2024 at 3:32 AM
Aw, yeah! It’s en-dash time, people!
Hallo! Does anyone have a robust protocol for coupling DNA oligos to proteins? The oligo will be fairly long, and so protocols that require lower mass inputs are preferred. We have free-rein over the oligo chemistry, but the protein is from a commercial source. Feel free to PM. Thanks in advance! 🙏
December 6, 2024 at 9:16 PM
Hallo! Does anyone have a robust protocol for coupling DNA oligos to proteins? The oligo will be fairly long, and so protocols that require lower mass inputs are preferred. We have free-rein over the oligo chemistry, but the protein is from a commercial source. Feel free to PM. Thanks in advance! 🙏
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..
December 5, 2024 at 2:20 AM
Just updated my phone OS, and apparently the calendar doubles as a karyotype now?
November 30, 2024 at 6:40 PM
Just updated my phone OS, and apparently the calendar doubles as a karyotype now?
Oh, it’s ON now. Welcome, welcome, @lippilab.bsky.social!
November 29, 2024 at 11:26 PM
Oh, it’s ON now. Welcome, welcome, @lippilab.bsky.social!
🤯🤯🤯
ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY.
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Ångström-resolution fluorescence microscopy - Nature
The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...
www.nature.com
November 29, 2024 at 5:05 AM
🤯🤯🤯
In a world where you can be anything, be Reviewer #1.
November 26, 2024 at 11:29 PM
In a world where you can be anything, be Reviewer #1.
Also, today I learned that the colloquial Turkish term for puberty is, “Delikanlık,” which literally translates to, “Crazy Blood-ness.”
I assert once again that Turkish is the perfect language and none of you will be able to convince me otherwise.
I assert once again that Turkish is the perfect language and none of you will be able to convince me otherwise.
November 24, 2024 at 7:33 PM
Also, today I learned that the colloquial Turkish term for puberty is, “Delikanlık,” which literally translates to, “Crazy Blood-ness.”
I assert once again that Turkish is the perfect language and none of you will be able to convince me otherwise.
I assert once again that Turkish is the perfect language and none of you will be able to convince me otherwise.
It’s national Teachers’ Appreciation Day in Türkiye, and to all the Turkish teachers out there, Öğretmenler Günü Çok Kutlu olsun!!!
November 24, 2024 at 7:30 PM
It’s national Teachers’ Appreciation Day in Türkiye, and to all the Turkish teachers out there, Öğretmenler Günü Çok Kutlu olsun!!!
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
November 21, 2024 at 1:27 AM
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
Woohooo! Fantastic seeing this out in print. Congrats to all the authors—it was an honor to play a small part in this exciting work!
The pSABER paper is now online!
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods
pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...
doi.org
November 15, 2024 at 10:29 PM
Woohooo! Fantastic seeing this out in print. Congrats to all the authors—it was an honor to play a small part in this exciting work!
This is an outrage, I tell you! AN OUTRAGE!!
November 15, 2024 at 6:00 PM
This is an outrage, I tell you! AN OUTRAGE!!
Life goal attained. 🥹
November 15, 2024 at 7:34 AM
Life goal attained. 🥹
Woot! Welcome to the party, @yasesancak.bsky.social !!
November 15, 2024 at 7:30 AM
Woot! Welcome to the party, @yasesancak.bsky.social !!
Woot! Welcome to the party, @aliceyting.bsky.social!
Great to see Stanford colleague and chemical biologist extraordaire @aliceyting.bsky.social here at 🦋 😀
November 14, 2024 at 7:04 PM
Woot! Welcome to the party, @aliceyting.bsky.social!
YES! More love for the HLB.
📣 New preprint! Pol II forms clusters, some of which are giant: Pol II clusters at fly histone genes could fill a yeast nucleus!👇How do these structures regulate transcription? This is what
Feiyue Lu, a joint postdoc w Ruth Lehmann, looked into 1/6 biorxiv.org/content/10.1...
Feiyue Lu, a joint postdoc w Ruth Lehmann, looked into 1/6 biorxiv.org/content/10.1...
November 14, 2024 at 7:34 AM
YES! More love for the HLB.