Hana Polasek-Sedlackova
@hanasedlackova.bsky.social
Group leader at the Institute of Biophysics, Czech Academy of Sciences | Czech Science Foundation Junior Star fellow | ERA & EMBO IG fellow | Deciphering DNA replication & genome integrity in normal & cancer development.
Truly delighted to host @agagambus.bsky.social next week in Brno for an IBP Guest Lecture! Her groundbreaking work and genuinely human approach to science have always been a great inspiration to me. Come and join us for her lecture at IBP 👇
November 14, 2025 at 8:00 PM
Truly delighted to host @agagambus.bsky.social next week in Brno for an IBP Guest Lecture! Her groundbreaking work and genuinely human approach to science have always been a great inspiration to me. Come and join us for her lecture at IBP 👇
📢 Excited to share that our study has just been published in Nature Communications! We uncover how CRL4-DCAF12 regulates the cellular levels of MCMBP, a chaperone essential for assembling nascent MCM2-7 complexes, to ensure accurate and error-free genome duplication.
October 28, 2025 at 3:04 PM
📢 Excited to share that our study has just been published in Nature Communications! We uncover how CRL4-DCAF12 regulates the cellular levels of MCMBP, a chaperone essential for assembling nascent MCM2-7 complexes, to ensure accurate and error-free genome duplication.
Reposted by Hana Polasek-Sedlackova
A comprehensive genetic catalog of human double-strand break repair | Science www.science.org/doi/10.1126/...
A comprehensive genetic catalog of human double-strand break repair
The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...
www.science.org
October 2, 2025 at 6:47 PM
A comprehensive genetic catalog of human double-strand break repair | Science www.science.org/doi/10.1126/...
Reposted by Hana Polasek-Sedlackova
@natsmb.nature.com has published three pieces on scientists returning to their country of origin after working abroad. www.nature.com/nsmb/article... Two more will come out. The most recent one by @hanasedlackova.bsky.social hits home for me, a very interesting read.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Returning home: there and back again in Czechia - Nature Structural & Molecular Biology
Returning to Central Europe was appealing and challenging. Here, I reflect on my part in rebuilding a research career in Czechia and fostering a collaborative scientific community across the region.
www.nature.com
August 12, 2025 at 11:54 AM
@natsmb.nature.com has published three pieces on scientists returning to their country of origin after working abroad. www.nature.com/nsmb/article... Two more will come out. The most recent one by @hanasedlackova.bsky.social hits home for me, a very interesting read.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Hana Polasek-Sedlackova
ICYMI: New online: Returning home: there and back again in Czechia
Returning home: there and back again in Czechia
Nature Structural & Molecular Biology, Published online: 07 August 2025; doi:10.1038/s41594-025-01642-1Returning to Central Europe was appealing and challenging. Here, I reflect on my part in rebuilding a research career in Czechia and fostering a collaborative scientific community across the region.
go.nature.com
August 8, 2025 at 11:01 AM
ICYMI: New online: Returning home: there and back again in Czechia
📢 Excited to share my World View article, just published in @NatureSMB, where I reflect on returning to Czechia and building research group grounded in collaboration🤝, resilience and curiosity💡. www.nature.com/articles/s41...
https://nature.com/articles/s4159…
August 7, 2025 at 5:28 PM
📢 Excited to share my World View article, just published in @NatureSMB, where I reflect on returning to Czechia and building research group grounded in collaboration🤝, resilience and curiosity💡. www.nature.com/articles/s41...
Reposted by Hana Polasek-Sedlackova
🧑🔬🎙️ When you publish with JCB, you can reach more of your community by submitting a short video! Want to promote your research in your own voice? 🎦 See our guidelines: rupress.org/jcb/pages/vi...
▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol
▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol
August 4, 2025 at 3:30 PM
🧑🔬🎙️ When you publish with JCB, you can reach more of your community by submitting a short video! Want to promote your research in your own voice? 🎦 See our guidelines: rupress.org/jcb/pages/vi...
▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol
▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol
Reposted by Hana Polasek-Sedlackova
Another reason to consider HaloTag as an alternative to GFP for tagging
A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/...
#Technology #Chromatin #epigenetics #CellCycle
#Technology #Chromatin #epigenetics #CellCycle
July 23, 2025 at 9:07 PM
Another reason to consider HaloTag as an alternative to GFP for tagging
Reposted by Hana Polasek-Sedlackova
We're very proud that our recent PhD graduate (and now postdoc) Rosie Wilkins' study on RNase H2 overexpression in human cells has been published in Oncogene.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress - Oncogene
Oncogene - Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress
www.nature.com
July 23, 2025 at 12:39 PM
We're very proud that our recent PhD graduate (and now postdoc) Rosie Wilkins' study on RNase H2 overexpression in human cells has been published in Oncogene.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Hana Polasek-Sedlackova
A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/...
#Technology #Chromatin #epigenetics #CellCycle
#Technology #Chromatin #epigenetics #CellCycle
July 23, 2025 at 1:59 PM
A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/...
#Technology #Chromatin #epigenetics #CellCycle
#Technology #Chromatin #epigenetics #CellCycle
Reposted by Hana Polasek-Sedlackova
Thank you, Melina, for the interview!
July 22, 2025 at 7:27 AM
Thank you, Melina, for the interview!
📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...
July 23, 2025 at 10:10 AM
📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...
Reposted by Hana Polasek-Sedlackova
Yadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
July 16, 2025 at 4:15 PM
Yadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
Reposted by Hana Polasek-Sedlackova
In @jcb.org, the @hanasedlackova.bsky.social lab presents a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
July 16, 2025 at 4:15 PM
In @jcb.org, the @hanasedlackova.bsky.social lab presents a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...
Reposted by Hana Polasek-Sedlackova
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS
Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic
academic.oup.com
July 10, 2025 at 2:55 PM
It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...
Reposted by Hana Polasek-Sedlackova
Apply to our PhD program!!
🧬 Looking for a PhD position in molecular or developmental biology? 🔬
✉️ The Hubrecht Institute now has its very own PhD program! Applications are open and will be accepted until September 15th. Read more here 👉 www.hubrecht.eu/hipp/
✉️ The Hubrecht Institute now has its very own PhD program! Applications are open and will be accepted until September 15th. Read more here 👉 www.hubrecht.eu/hipp/
July 10, 2025 at 6:56 PM
Apply to our PhD program!!
Reposted by Hana Polasek-Sedlackova
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted by Hana Polasek-Sedlackova
This review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs. doi.org/10.1038/s420...
Cellular parameters shaping pathways of targeted protein degradation - Communications Biology
This review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs.
doi.org
June 27, 2025 at 3:05 PM
This review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs. doi.org/10.1038/s420...
Reposted by Hana Polasek-Sedlackova
This editorial details why we think it's a good idea to standarise reporting of microscopy data and what we are doing towards that. I also feel that it is both important and a step towards helping the community.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reporting light microscopy data in our pages - Nature Structural & Molecular Biology
We outline a pilot starting at several Nature Portfolio journals aimed at improving and standardizing the reporting of light and fluorescence microscopy experiments.
www.nature.com
June 19, 2025 at 6:59 PM
This editorial details why we think it's a good idea to standarise reporting of microscopy data and what we are doing towards that. I also feel that it is both important and a step towards helping the community.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Hana Polasek-Sedlackova
Postdoc positions open in my lab (Francis Crick Institute) to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
June 20, 2025 at 9:38 AM
Postdoc positions open in my lab (Francis Crick Institute) to study
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
1. initiation of DNA replication.
2. chromatin replication/epigenetic inheritance.
Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.
Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
Reposted by Hana Polasek-Sedlackova
If you are interested in working on chromatin and oogenesis in the mouse in beautiful Copenhagen, we are recruiting a postdoc!
jobportal.ku.dk/videnskabeli...
Please share!
jobportal.ku.dk/videnskabeli...
Please share!
Beetween
app.beetween.com
June 17, 2025 at 1:24 PM
If you are interested in working on chromatin and oogenesis in the mouse in beautiful Copenhagen, we are recruiting a postdoc!
jobportal.ku.dk/videnskabeli...
Please share!
jobportal.ku.dk/videnskabeli...
Please share!
Reposted by Hana Polasek-Sedlackova
📣Check out our recent review (written with Martina Huranova lab) on converging mechanisms controlling #cilia #dynamics during #cell #cycle, #cell #signaling, and cross-talk of ciliary modules.
rdcu.be/epVDc
rdcu.be/epVDc
Cilia at the crossroad: convergence of regulatory mechanisms to govern cilia dynamics during cell signaling and the cell cycle
rdcu.be
June 10, 2025 at 6:35 AM
📣Check out our recent review (written with Martina Huranova lab) on converging mechanisms controlling #cilia #dynamics during #cell #cycle, #cell #signaling, and cross-talk of ciliary modules.
rdcu.be/epVDc
rdcu.be/epVDc
Reposted by Hana Polasek-Sedlackova
So excited to share that we @natsmb.nature.com are hiring a new editor! If you are interested about working with an intelligent, compassionate and funny team helping scientists publish great research, please consider applying. Deadline June 20th. springernature.wd3.myworkdayjobs.com/SpringerNatu...
Associate or Senior Editor (Nature Structural & Molecular Biology and Nature Communications)
Job Title: Associate or Senior Editor (Nature Structural & Molecular Biology and Nature Communications) Location: New York, Shanghai, Beijing, Madrid or Milan Closing date: June 20th About Springe...
springernature.wd3.myworkdayjobs.com
June 10, 2025 at 6:44 PM
So excited to share that we @natsmb.nature.com are hiring a new editor! If you are interested about working with an intelligent, compassionate and funny team helping scientists publish great research, please consider applying. Deadline June 20th. springernature.wd3.myworkdayjobs.com/SpringerNatu...
Reposted by Hana Polasek-Sedlackova
Next Tuesday's webinar will feature @agagambus.bsky.social and @chowdhurylab.bsky.social. Join us on June 3rd at 5PM Rome / 11AM NYC for a lively discussion on DNA replication and repair!
Zoom link: uniroma1.zoom.us/j/95149984403
Become part of our community: docs.google.com/forms/d/e/1F...
Zoom link: uniroma1.zoom.us/j/95149984403
Become part of our community: docs.google.com/forms/d/e/1F...
May 28, 2025 at 7:21 PM
Next Tuesday's webinar will feature @agagambus.bsky.social and @chowdhurylab.bsky.social. Join us on June 3rd at 5PM Rome / 11AM NYC for a lively discussion on DNA replication and repair!
Zoom link: uniroma1.zoom.us/j/95149984403
Become part of our community: docs.google.com/forms/d/e/1F...
Zoom link: uniroma1.zoom.us/j/95149984403
Become part of our community: docs.google.com/forms/d/e/1F...
Reposted by Hana Polasek-Sedlackova
Oh wow. Starting grant 0-10 years post PhD from 2027, Consolidator 5-15
Planning to apply for #research #funding from the ERC?
From the next application rounds, expect changes to the:
• proposal structure
• evaluation process
• extra funding you can request
• eligibility for Starting & Consolidator #Grants (from 2027)
More 👇 europa.eu/!RPHWvv
From the next application rounds, expect changes to the:
• proposal structure
• evaluation process
• extra funding you can request
• eligibility for Starting & Consolidator #Grants (from 2027)
More 👇 europa.eu/!RPHWvv
Changes to the 2026 and 2027 Work Programmes
With the launch of the competitions for grants under ERC Work Programme 2026 in July of this year, several changes to the submission of applications and the evaluation of proposals will apply. The mai...
europa.eu
June 2, 2025 at 12:17 PM
Oh wow. Starting grant 0-10 years post PhD from 2027, Consolidator 5-15