Lucien Hinderling
@lhinderling.bsky.social
tries to make microscopes smarter · bioimage analysis, optogenetics, ml, 3d printing, open science · phd student in cellular signalling dynamics @PertzLab
Pinned
Automated optogenetic control of hundreds of cells in parallel. Each cell is individually steered, collectively acting as a "tissue printer". Preprint & code out! www.biorxiv.org/content/10.1...
Reposted by Lucien Hinderling
Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2)
forum.image.sc/t/bigvolumeb...
forum.image.sc/t/bigvolumeb...
BigVolumeBrowser: a new 3D multi volume/mesh/point clould (SMLM) data viewer
Hello everyone, I’d like to share with you another 3D viewer for FIJI, BigVolumeBrowser (full documentation link). It‘s a first initial public release, so there is still space for improvements. Le...
forum.image.sc
November 21, 2025 at 9:29 AM
Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2)
forum.image.sc/t/bigvolumeb...
forum.image.sc/t/bigvolumeb...
Reposted by Lucien Hinderling
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
Reposted by Lucien Hinderling
Excited to share a new preprint! Wireless devices use FM modulation to transmit multiplexed noise-resistant data. Led by @born2raisecell.bsky.social, we create a biochemical analogue of this paradigm using genetically encoded oscillators (GEOs) for single-cell FM streaming tinyurl.com/nbs8rw42 🧵
March 4, 2025 at 4:28 PM
Excited to share a new preprint! Wireless devices use FM modulation to transmit multiplexed noise-resistant data. Led by @born2raisecell.bsky.social, we create a biochemical analogue of this paradigm using genetically encoded oscillators (GEOs) for single-cell FM streaming tinyurl.com/nbs8rw42 🧵
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
November 19, 2025 at 8:15 PM
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
Reposted by Lucien Hinderling
The new open source syringe pump control boards arrived. Exciting!
November 18, 2025 at 12:51 PM
The new open source syringe pump control boards arrived. Exciting!
Reposted by Lucien Hinderling
The first meeting was a blast and the second one promises to be even better - if you submit your abstract and join us!
Join us for the second edition of 'AI and biology'! 🤖🧬
Explore how AI transforms biological research – from foundation models for biology to generative modelling.
10 – 13 March 2026
EMBL Heidelberg and Virtual
Submit your abstract by 2 Dec: s.embl.org/ees26-02-bl
#EESAIBio #AI
Explore how AI transforms biological research – from foundation models for biology to generative modelling.
10 – 13 March 2026
EMBL Heidelberg and Virtual
Submit your abstract by 2 Dec: s.embl.org/ees26-02-bl
#EESAIBio #AI
November 18, 2025 at 12:43 PM
The first meeting was a blast and the second one promises to be even better - if you submit your abstract and join us!
Last-minute advert for the Convpaint workshop at I2K by Roman Schwob! Interactive segmentation in @napari.org. Today 2:00 PM CET. Free registration:
www.i2kconference.org/workshops
www.i2kconference.org/workshops
November 18, 2025 at 12:13 PM
Last-minute advert for the Convpaint workshop at I2K by Roman Schwob! Interactive segmentation in @napari.org. Today 2:00 PM CET. Free registration:
www.i2kconference.org/workshops
www.i2kconference.org/workshops
Reposted by Lucien Hinderling
🚨New paper! 🚨
@jasminealqassar.bsky.social led this work on the silk glands of the pantry moth.
These two long tubes inside the caterpillar continuously make a ton of silk
How does this special organ work?
www.cell.com/iscience/ful...
@cp-iscience.bsky.social
🧵THREAD🧵
@jasminealqassar.bsky.social led this work on the silk glands of the pantry moth.
These two long tubes inside the caterpillar continuously make a ton of silk
How does this special organ work?
www.cell.com/iscience/ful...
@cp-iscience.bsky.social
🧵THREAD🧵
November 16, 2025 at 2:06 AM
🚨New paper! 🚨
@jasminealqassar.bsky.social led this work on the silk glands of the pantry moth.
These two long tubes inside the caterpillar continuously make a ton of silk
How does this special organ work?
www.cell.com/iscience/ful...
@cp-iscience.bsky.social
🧵THREAD🧵
@jasminealqassar.bsky.social led this work on the silk glands of the pantry moth.
These two long tubes inside the caterpillar continuously make a ton of silk
How does this special organ work?
www.cell.com/iscience/ful...
@cp-iscience.bsky.social
🧵THREAD🧵
Reposted by Lucien Hinderling
We had an amazing OME-NGFF hackathon last week, Wednesday to Friday, following the inspiring OME-NGFF symposium. What a fantastic week! It’s hard to describe how motivating it is to be surrounded by so many talented, curious & friendly people who care deeply about open, FAIR bioimaging data.
1/14 🧵
1/14 🧵
November 17, 2025 at 1:24 PM
We had an amazing OME-NGFF hackathon last week, Wednesday to Friday, following the inspiring OME-NGFF symposium. What a fantastic week! It’s hard to describe how motivating it is to be surrounded by so many talented, curious & friendly people who care deeply about open, FAIR bioimaging data.
1/14 🧵
1/14 🧵
Reposted by Lucien Hinderling
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Lucien Hinderling
Dish soap. Seriously. 😆
November 12, 2025 at 7:06 AM
Dish soap. Seriously. 😆
Reposted by Lucien Hinderling
Felix Zhou's u-Segment3D is out:
www.nature.com/articles/s41...
It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.
It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.
www.nature.com/articles/s41...
It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.
It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.
November 12, 2025 at 2:41 AM
Felix Zhou's u-Segment3D is out:
www.nature.com/articles/s41...
It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.
It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.
www.nature.com/articles/s41...
It leverages 2D cell segmentations from orthoviews to generate a ‘consensus’ 3D segmentation.
It does a great job on segmenting densely packed samples, and we have used the algorithm in many applications.
Reposted by Lucien Hinderling
Check out this sneaky lamin A knockdown cell (pink dot) swimming against the tide (migration into gap) & slithering between its sisters.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
November 7, 2025 at 4:29 PM
Check out this sneaky lamin A knockdown cell (pink dot) swimming against the tide (migration into gap) & slithering between its sisters.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
Reposted by Lucien Hinderling
Hi Fans of Feedback Microscopy,
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
November 7, 2025 at 7:23 PM
Hi Fans of Feedback Microscopy,
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
what would you do when you have access to the pixel stream from @zeiss-microscopy.bsky.social inside @napari.org?
Btw, it works as well in Fiji&ImageJ :-)
Sidenote: Experiments started from "outside" do not update the ZEN UI. But they are executed by ZENservice.
Reposted by Lucien Hinderling
Intestinal villus cells extrude living, following a calcium pulse. Unlike from extruding apoptotic cells, Ca2+ messaging stops at the first neighbors! #FluorescenceFriday
Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...
Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social
Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...
Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social
November 7, 2025 at 2:40 PM
Intestinal villus cells extrude living, following a calcium pulse. Unlike from extruding apoptotic cells, Ca2+ messaging stops at the first neighbors! #FluorescenceFriday
Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...
Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social
Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...
Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social
Reposted by Lucien Hinderling
A bit delayed, but excited to still share our latest paper, showing that intercellular forces transduced by E-cadherin activate EGFR-ERK signaling in epithelia by inducing EGFR ligand shedding! Mechanical and biochemical signals can act together within a single, linear cascade! tinyurl.com/mr9mj9j2
E-cadherin mechanotransduction activates EGFR-ERK signaling in epithelial monolayers by inducing ADAM-mediated ligand shedding
Epithelial stretching promotes the release of EGF receptor ligands that stimulate ERK activation.
tinyurl.com
September 13, 2025 at 6:05 PM
A bit delayed, but excited to still share our latest paper, showing that intercellular forces transduced by E-cadherin activate EGFR-ERK signaling in epithelia by inducing EGFR ligand shedding! Mechanical and biochemical signals can act together within a single, linear cascade! tinyurl.com/mr9mj9j2
Reposted by Lucien Hinderling
We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!
www.sarstedt.com/en/products/...
www.sarstedt.com/en/products/...
October 28, 2025 at 8:27 PM
We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!
www.sarstedt.com/en/products/...
www.sarstedt.com/en/products/...
Reposted by Lucien Hinderling
We are planning the build of a light sheet setup and are considering a Powell lens to generate the sheet.
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
November 3, 2025 at 12:43 PM
We are planning the build of a light sheet setup and are considering a Powell lens to generate the sheet.
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
Reposted by Lucien Hinderling
What a gem from @dudinlab.bsky.social @gautamdey.bsky.social @centriolelab.bsky.social in Cell! Expansion microscopy atlas of >200 eukaryotes comparing cytoskeletal architectures revealing structures not seen before. Stunning visualisation! Exactly the kind of transformative cell biology we need.
October 31, 2025 at 5:45 PM
What a gem from @dudinlab.bsky.social @gautamdey.bsky.social @centriolelab.bsky.social in Cell! Expansion microscopy atlas of >200 eukaryotes comparing cytoskeletal architectures revealing structures not seen before. Stunning visualisation! Exactly the kind of transformative cell biology we need.
Reposted by Lucien Hinderling
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
October 31, 2025 at 2:42 PM
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
microfabricated stamps, fresh off the printer :)
October 26, 2025 at 4:24 PM
microfabricated stamps, fresh off the printer :)
Reposted by Lucien Hinderling
Reposted by Lucien Hinderling
Low-Cost 3D Printed Optics for Super-Resolution Multifocal Structured Illumination Microscopy https://www.biorxiv.org/content/10.1101/2025.10.09.680962v1
October 10, 2025 at 7:48 PM
Low-Cost 3D Printed Optics for Super-Resolution Multifocal Structured Illumination Microscopy https://www.biorxiv.org/content/10.1101/2025.10.09.680962v1
Reposted by Lucien Hinderling
Un-frigging-believable. I have waited for something like this for so long!
October 5, 2025 at 8:01 AM
Un-frigging-believable. I have waited for something like this for so long!