Ernest van der Wee
@evdwee.bsky.social
Microscopist | Biology Imaging Center | Utrecht University
Reposted by Ernest van der Wee
One of the perks of living in San Francisco is free visits to the Photonics West trade show. Sharing my notes for those missing out: nicost.github.io/microscope-t...
Photonics West 2026 impressions
The Photonics West conference includes a gigantic tradeshow spanning 2 halls of the Moscone Center and some of the space in between. I visited on two consecutive afternoon, trying to at least walk by ...
nicost.github.io
January 26, 2026 at 6:55 PM
One of the perks of living in San Francisco is free visits to the Photonics West trade show. Sharing my notes for those missing out: nicost.github.io/microscope-t...
Reposted by Ernest van der Wee
If you have time tomorrow, join my online talk about LLS/single molecule/microtubules and 3D data at @bioimagingna.bsky.social user group (18:00 CET for Europe).
I hope I manage to fit 25 movies into 20 cohesive slides and connection will be ok.
I hope I manage to fit 25 movies into 20 cohesive slides and connection will be ok.
#HappyMicroscpyMonday #microscopycommunity - Join us tomorrow, Tue Jan 6 for a talk by Eugene Katrukha on "Single molecule imaging using LLS7", hosted by the BINA Lattice Lightsheet User Group
Learn more & register here: buff.ly/US5Xd2q
Learn more & register here: buff.ly/US5Xd2q
January 5, 2026 at 7:36 PM
If you have time tomorrow, join my online talk about LLS/single molecule/microtubules and 3D data at @bioimagingna.bsky.social user group (18:00 CET for Europe).
I hope I manage to fit 25 movies into 20 cohesive slides and connection will be ok.
I hope I manage to fit 25 movies into 20 cohesive slides and connection will be ok.
Reposted by Ernest van der Wee
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
December 24, 2025 at 1:16 PM
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
Reposted by Ernest van der Wee
Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
December 24, 2025 at 7:18 AM
Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Reposted by Ernest van der Wee
Pfffffff. Chains of transformations are hard. It took me two releases to get the live deskew slider right and looking ok (hopefully) #bigvolumebrowser
December 22, 2025 at 10:40 PM
Pfffffff. Chains of transformations are hard. It took me two releases to get the live deskew slider right and looking ok (hopefully) #bigvolumebrowser
Reposted by Ernest van der Wee
My lab is hiring a postdoc (ERC funded)! If you're interested, get in touch!
December 18, 2025 at 8:36 AM
My lab is hiring a postdoc (ERC funded)! If you're interested, get in touch!
Reposted by Ernest van der Wee
Now published with peer-review-induced improvements in Biomedical Optics Express: doi.org/10.1364/BOE.579043
We continue to recommend the use of Sarstedt lumox dishes for ease of use and high-quality imaging performance: bsky.app/profile/jame...
We continue to recommend the use of Sarstedt lumox dishes for ease of use and high-quality imaging performance: bsky.app/profile/jame...
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
December 15, 2025 at 3:41 PM
Now published with peer-review-induced improvements in Biomedical Optics Express: doi.org/10.1364/BOE.579043
We continue to recommend the use of Sarstedt lumox dishes for ease of use and high-quality imaging performance: bsky.app/profile/jame...
We continue to recommend the use of Sarstedt lumox dishes for ease of use and high-quality imaging performance: bsky.app/profile/jame...
Reposted by Ernest van der Wee
The last bit is that it can also load and render lidar point cloud data. Not sure if it is beneficial for microscopy, but why not (4/4) bsky.app/profile/ekat...
Now with colors, the Dom Tower of Utrecht #BigVolumeBrowser @utrechtuniversity.bsky.social
December 12, 2025 at 10:19 AM
The last bit is that it can also load and render lidar point cloud data. Not sure if it is beneficial for microscopy, but why not (4/4) bsky.app/profile/ekat...
Reposted by Ernest van der Wee
For the (lattice) light sheet data, now there is an "on-the-fly" deskew mode with an arbitrary angle. So raw LLS7/OPM/Snouty data can be used. Gonna tag some folks, please try it @retof.bsky.social @kevin-dean.bsky.social @amsikking.bsky.social @microtubule.bsky.social @jsdaniel02.bsky.social (2/4)
December 12, 2025 at 10:13 AM
For the (lattice) light sheet data, now there is an "on-the-fly" deskew mode with an arbitrary angle. So raw LLS7/OPM/Snouty data can be used. Gonna tag some folks, please try it @retof.bsky.social @kevin-dean.bsky.social @amsikking.bsky.social @microtubule.bsky.social @jsdaniel02.bsky.social (2/4)
Reposted by Ernest van der Wee
New release of #BigVolumeBrowser. Many things were added: UI improvement (thanks to @kwolbachia.bsky.social feedback), pyramidization for BioFormats dataset, bug fixes.
Now there is an isosurface rendering mode (1/4)
Now there is an isosurface rendering mode (1/4)
December 12, 2025 at 10:10 AM
New release of #BigVolumeBrowser. Many things were added: UI improvement (thanks to @kwolbachia.bsky.social feedback), pyramidization for BioFormats dataset, bug fixes.
Now there is an isosurface rendering mode (1/4)
Now there is an isosurface rendering mode (1/4)
Reposted by Ernest van der Wee
Just look at those microtubules go! Happy to see our paper out in JCS ✨
Malina Iwanski @mkiwanski.bsky.social, Lukas Kapitein and colleagues discover that stable microtubules reverse their polarity during neuronal development.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
December 9, 2025 at 12:22 PM
Just look at those microtubules go! Happy to see our paper out in JCS ✨
Reposted by Ernest van der Wee
Malina Iwanski @mkiwanski.bsky.social, Lukas Kapitein and colleagues discover that stable microtubules reverse their polarity during neuronal development.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
December 8, 2025 at 3:51 PM
Malina Iwanski @mkiwanski.bsky.social, Lukas Kapitein and colleagues discover that stable microtubules reverse their polarity during neuronal development.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
Reposted by Ernest van der Wee
Pinpointing single molecules with a technique called motor-PAINT to analyse microtubule filament orientation & polarity at super resolution in developing neurons
📹 @mkiwanski.bsky.social et al Kapitein lab @utrechtuniversity.bsky.social in @jcellsci.bsky.social
➡️ bpod.org.uk/archive/2025...
📹 @mkiwanski.bsky.social et al Kapitein lab @utrechtuniversity.bsky.social in @jcellsci.bsky.social
➡️ bpod.org.uk/archive/2025...
December 3, 2025 at 9:26 AM
Pinpointing single molecules with a technique called motor-PAINT to analyse microtubule filament orientation & polarity at super resolution in developing neurons
📹 @mkiwanski.bsky.social et al Kapitein lab @utrechtuniversity.bsky.social in @jcellsci.bsky.social
➡️ bpod.org.uk/archive/2025...
📹 @mkiwanski.bsky.social et al Kapitein lab @utrechtuniversity.bsky.social in @jcellsci.bsky.social
➡️ bpod.org.uk/archive/2025...
Newest acquisition!
December 3, 2025 at 8:15 PM
Newest acquisition!
Reposted by Ernest van der Wee
Now with colors, the Dom Tower of Utrecht #BigVolumeBrowser @utrechtuniversity.bsky.social
December 3, 2025 at 3:13 PM
Now with colors, the Dom Tower of Utrecht #BigVolumeBrowser @utrechtuniversity.bsky.social
Reposted by Ernest van der Wee
Make your own sticker against #Fascism
Woody Guthrie used his music to fight in the 1940ies. Let his guitar be a reminder to us that we all have tools available to stand up.
As biologists, we are trained to See, Understand, Document, and Report - let's apply these today!
doi.org/10.6084/m9.f...
Woody Guthrie used his music to fight in the 1940ies. Let his guitar be a reminder to us that we all have tools available to stand up.
As biologists, we are trained to See, Understand, Document, and Report - let's apply these today!
doi.org/10.6084/m9.f...
November 25, 2025 at 3:26 PM
Make your own sticker against #Fascism
Woody Guthrie used his music to fight in the 1940ies. Let his guitar be a reminder to us that we all have tools available to stand up.
As biologists, we are trained to See, Understand, Document, and Report - let's apply these today!
doi.org/10.6084/m9.f...
Woody Guthrie used his music to fight in the 1940ies. Let his guitar be a reminder to us that we all have tools available to stand up.
As biologists, we are trained to See, Understand, Document, and Report - let's apply these today!
doi.org/10.6084/m9.f...
Reposted by Ernest van der Wee
Topical conclusion from @helenajambor.bsky.social
Scientists have tools to fight and make life better
#CBiAS2025
Scientists have tools to fight and make life better
#CBiAS2025
November 25, 2025 at 11:45 AM
Topical conclusion from @helenajambor.bsky.social
Scientists have tools to fight and make life better
#CBiAS2025
Scientists have tools to fight and make life better
#CBiAS2025
Highly recommended
Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2)
forum.image.sc/t/bigvolumeb...
forum.image.sc/t/bigvolumeb...
BigVolumeBrowser: a new 3D multi volume/mesh/point clould (SMLM) data viewer
Hello everyone, I’d like to share with you another 3D viewer for FIJI, BigVolumeBrowser (full documentation link). It‘s a first initial public release, so there is still space for improvements. Le...
forum.image.sc
November 21, 2025 at 10:27 AM
Highly recommended
Reposted by Ernest van der Wee
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
November 19, 2025 at 8:29 PM
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
We are planning the build of a light sheet setup and are considering a Powell lens to generate the sheet.
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
November 3, 2025 at 12:43 PM
We are planning the build of a light sheet setup and are considering a Powell lens to generate the sheet.
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
I've not been able to find a discussion of the pros and cons in literature, so if you ever used a Powell lens or have considered using one in your setup, I would love to hear your opinion. THX!
Reposted by Ernest van der Wee
We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!
www.sarstedt.com/en/products/...
www.sarstedt.com/en/products/...
October 28, 2025 at 8:27 PM
We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!
www.sarstedt.com/en/products/...
www.sarstedt.com/en/products/...
Reposted by Ernest van der Wee
Internet people, please help me to decide on the logo for #bigvolumebrowser, a multi-volume 3D viewer plugin for Fiji I'm working on. Please vote by replying with a number or liking one that was already mentioned. My favorites are 6, 7, 8 and 10 so far.
October 28, 2025 at 1:55 PM
Internet people, please help me to decide on the logo for #bigvolumebrowser, a multi-volume 3D viewer plugin for Fiji I'm working on. Please vote by replying with a number or liking one that was already mentioned. My favorites are 6, 7, 8 and 10 so far.
PhD opportunity at Utrecht University in the lab of @tingfenglin.bsky.social !
www.uu.nl/en/organisat...
If you are interested in neuroscience and building a microscope: don't hesitate to apply!
Please forward this to people who might be interested.
www.uu.nl/en/organisat...
If you are interested in neuroscience and building a microscope: don't hesitate to apply!
Please forward this to people who might be interested.
PhD position in Neuroscience
Join us in dissecting neuronal mechanisms underlying learning and memory through cutting-edge microscopy.
www.uu.nl
October 3, 2025 at 1:58 PM
PhD opportunity at Utrecht University in the lab of @tingfenglin.bsky.social !
www.uu.nl/en/organisat...
If you are interested in neuroscience and building a microscope: don't hesitate to apply!
Please forward this to people who might be interested.
www.uu.nl/en/organisat...
If you are interested in neuroscience and building a microscope: don't hesitate to apply!
Please forward this to people who might be interested.
Reposted by Ernest van der Wee
Hi #Neuroscience,
Exciting news—I’m recruiting a PhD student to join my lab and study neuroscience in larval zebrafish 🧠🐟
Microscopy expertise is a big plus!
Link: drive.google.com/file/d/1Wb4h...
Exciting news—I’m recruiting a PhD student to join my lab and study neuroscience in larval zebrafish 🧠🐟
Microscopy expertise is a big plus!
Link: drive.google.com/file/d/1Wb4h...
NeuroPhD vacancy FoS - TFL2025.pdf
drive.google.com
September 25, 2025 at 9:53 PM
Hi #Neuroscience,
Exciting news—I’m recruiting a PhD student to join my lab and study neuroscience in larval zebrafish 🧠🐟
Microscopy expertise is a big plus!
Link: drive.google.com/file/d/1Wb4h...
Exciting news—I’m recruiting a PhD student to join my lab and study neuroscience in larval zebrafish 🧠🐟
Microscopy expertise is a big plus!
Link: drive.google.com/file/d/1Wb4h...