Isletomics
@isletomics.bsky.social
Sugar stimulated and oozing goodness
Reposted by Isletomics
#SpatialTranscriptomics #Atherosclerosis
A first CosMx 1k plex single-cell map of👤coronary atherosclerosis😆
From Adaptive intimal thickening to Large Atheromatous plaque with intralesional fibrosis
bioRxiv 2025
www.biorxiv.org/content/10.1...
A first CosMx 1k plex single-cell map of👤coronary atherosclerosis😆
From Adaptive intimal thickening to Large Atheromatous plaque with intralesional fibrosis
bioRxiv 2025
www.biorxiv.org/content/10.1...
April 6, 2025 at 11:16 AM
#SpatialTranscriptomics #Atherosclerosis
A first CosMx 1k plex single-cell map of👤coronary atherosclerosis😆
From Adaptive intimal thickening to Large Atheromatous plaque with intralesional fibrosis
bioRxiv 2025
www.biorxiv.org/content/10.1...
A first CosMx 1k plex single-cell map of👤coronary atherosclerosis😆
From Adaptive intimal thickening to Large Atheromatous plaque with intralesional fibrosis
bioRxiv 2025
www.biorxiv.org/content/10.1...
Reposted by Isletomics
#CircularRNA #Atherosclerosis
Human Aortic plaque vs control intima
15⏫ 13⏬
circLARP1B-encoded 243 aa peptide
⏫PDE4c
⏬cAMP-PKA
⏫#SmoothMuscleCell proliferation migration
Can cAMP be detected via WB (Fig 4B 5A)???😛
#Atherosclerosis 2024
www.sciencedirect.com/science/arti...
Human Aortic plaque vs control intima
15⏫ 13⏬
circLARP1B-encoded 243 aa peptide
⏫PDE4c
⏬cAMP-PKA
⏫#SmoothMuscleCell proliferation migration
Can cAMP be detected via WB (Fig 4B 5A)???😛
#Atherosclerosis 2024
www.sciencedirect.com/science/arti...
April 4, 2025 at 7:31 PM
#CircularRNA #Atherosclerosis
Human Aortic plaque vs control intima
15⏫ 13⏬
circLARP1B-encoded 243 aa peptide
⏫PDE4c
⏬cAMP-PKA
⏫#SmoothMuscleCell proliferation migration
Can cAMP be detected via WB (Fig 4B 5A)???😛
#Atherosclerosis 2024
www.sciencedirect.com/science/arti...
Human Aortic plaque vs control intima
15⏫ 13⏬
circLARP1B-encoded 243 aa peptide
⏫PDE4c
⏬cAMP-PKA
⏫#SmoothMuscleCell proliferation migration
Can cAMP be detected via WB (Fig 4B 5A)???😛
#Atherosclerosis 2024
www.sciencedirect.com/science/arti...
Reposted by Isletomics
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data www.nature.com/articles/s4...
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data
Nature Protocols - CellPhoneDB combines a curated database of receptor–ligand interactions with methods to infer cell–cell communication from single-cell expression data. This protocol...
www.nature.com
April 3, 2025 at 1:45 PM
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data www.nature.com/articles/s4...
Reposted by Isletomics
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry: Cell www.cell.com/cell/fulltex...
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry
This work challenges a major assumption in cell biology by showing that lipid bilayers
can have drastically different phospholipid abundances between their two leaflets.
This lipid abundance asymmetry...
www.cell.com
April 3, 2025 at 1:33 PM
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry: Cell www.cell.com/cell/fulltex...
Reposted by Isletomics
Cdc42 coordinates PDGFRb+ Mural Cell migration proliferation morphogenesis (wrapping filopodia) during postanatal retinal #Angiogenesis👁️
PDGFRb+ mural cell Cdc42 KO🐭➡️
⏫Angiogenic front leakage
⏬Tip cell filopodia
Perturbed arterial patterning
#VasPharma 2025
www.sciencedirect.com/science/arti...
PDGFRb+ mural cell Cdc42 KO🐭➡️
⏫Angiogenic front leakage
⏬Tip cell filopodia
Perturbed arterial patterning
#VasPharma 2025
www.sciencedirect.com/science/arti...
March 25, 2025 at 8:11 PM
Cdc42 coordinates PDGFRb+ Mural Cell migration proliferation morphogenesis (wrapping filopodia) during postanatal retinal #Angiogenesis👁️
PDGFRb+ mural cell Cdc42 KO🐭➡️
⏫Angiogenic front leakage
⏬Tip cell filopodia
Perturbed arterial patterning
#VasPharma 2025
www.sciencedirect.com/science/arti...
PDGFRb+ mural cell Cdc42 KO🐭➡️
⏫Angiogenic front leakage
⏬Tip cell filopodia
Perturbed arterial patterning
#VasPharma 2025
www.sciencedirect.com/science/arti...
Reposted by Isletomics
"On October 8, 1931, a law went into effect requiring every Italian university professor to sign an oath pledging their loyalty to the government of Benito Mussolini. Out of over 1,200 professors in the country, only 12 refused. All of them were immediately fired." www.chronicle.com/article/what...
Opinion | What Autocrats Want From Academics: Servility
In 1931, Italian scholars were made to take loyalty oaths. Will that happen to us?
www.chronicle.com
March 21, 2025 at 10:45 PM
"On October 8, 1931, a law went into effect requiring every Italian university professor to sign an oath pledging their loyalty to the government of Benito Mussolini. Out of over 1,200 professors in the country, only 12 refused. All of them were immediately fired." www.chronicle.com/article/what...
Reposted by Isletomics
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
March 21, 2025 at 2:09 PM
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
Reposted by Isletomics
Happy #FluorescenceFriday! Here is one from the vault circa 2018. These are cultured mouse fibroblast cells making contact with their neighbors through long cellular extensions. Have a great weekend! 🧪 🔬
March 21, 2025 at 8:22 PM
Happy #FluorescenceFriday! Here is one from the vault circa 2018. These are cultured mouse fibroblast cells making contact with their neighbors through long cellular extensions. Have a great weekend! 🧪 🔬
Reposted by Isletomics
In-cell architecture of the mitochondrial respiratory chain | Science www.science.org/doi/10.1126/...
In-cell architecture of the mitochondrial respiratory chain
Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, workin...
www.science.org
March 21, 2025 at 9:33 AM
In-cell architecture of the mitochondrial respiratory chain | Science www.science.org/doi/10.1126/...
Reposted by Isletomics
A constitutive #SmoothMuscleCell specific Cre mice under precise control of endogenous Myh11 loci🤠
This time, we do not need to worry about off-targeting embryonic cardiomyocyte or male gamete😁
Jiliang Zhou lab bioRxiv 2024
www.biorxiv.org/content/10.1...
This time, we do not need to worry about off-targeting embryonic cardiomyocyte or male gamete😁
Jiliang Zhou lab bioRxiv 2024
www.biorxiv.org/content/10.1...
March 21, 2025 at 6:52 PM
A constitutive #SmoothMuscleCell specific Cre mice under precise control of endogenous Myh11 loci🤠
This time, we do not need to worry about off-targeting embryonic cardiomyocyte or male gamete😁
Jiliang Zhou lab bioRxiv 2024
www.biorxiv.org/content/10.1...
This time, we do not need to worry about off-targeting embryonic cardiomyocyte or male gamete😁
Jiliang Zhou lab bioRxiv 2024
www.biorxiv.org/content/10.1...
Reposted by Isletomics
3D #SpatialMultiomics on the way!😍
Cycle Hybridization Chain Reaction
14 bp split L+R DNA barcodes
3 color channels
Cellpose-based 3D nucleus segmentation
120 RNA probes + 8 antibodies
~50 µm hippocampal slice
44 imaging cycles over 11 days
@science.org 2025
www.science.org/doi/10.1126/...
Cycle Hybridization Chain Reaction
14 bp split L+R DNA barcodes
3 color channels
Cellpose-based 3D nucleus segmentation
120 RNA probes + 8 antibodies
~50 µm hippocampal slice
44 imaging cycles over 11 days
@science.org 2025
www.science.org/doi/10.1126/...
March 20, 2025 at 10:44 AM
3D #SpatialMultiomics on the way!😍
Cycle Hybridization Chain Reaction
14 bp split L+R DNA barcodes
3 color channels
Cellpose-based 3D nucleus segmentation
120 RNA probes + 8 antibodies
~50 µm hippocampal slice
44 imaging cycles over 11 days
@science.org 2025
www.science.org/doi/10.1126/...
Cycle Hybridization Chain Reaction
14 bp split L+R DNA barcodes
3 color channels
Cellpose-based 3D nucleus segmentation
120 RNA probes + 8 antibodies
~50 µm hippocampal slice
44 imaging cycles over 11 days
@science.org 2025
www.science.org/doi/10.1126/...
Reposted by Isletomics
How does sleep protect against neurodegenerative disease? A review, open-access
www.cell.com/neuron/fullt...
www.cell.com/neuron/fullt...
March 19, 2025 at 3:44 PM
How does sleep protect against neurodegenerative disease? A review, open-access
www.cell.com/neuron/fullt...
www.cell.com/neuron/fullt...
Reposted by Isletomics
Review: Decoding cell–cell communication using spatial transcriptomics www.nature.com/articles/s4...
Decoding cell–cell communication using spatial transcriptomics
Nature Reviews Genetics - In this Tools of the Trade article, Ankit Agrawal introduces the computational framework NiCo (Niche Covariation), which integrates spatial transcriptomics with...
www.nature.com
March 19, 2025 at 1:45 PM
Review: Decoding cell–cell communication using spatial transcriptomics www.nature.com/articles/s4...
Reposted by Isletomics
The thing that bugs me the most with the NIH is the lack of any public communication from any of its leaders about what is happening.
March 2, 2025 at 2:17 AM
The thing that bugs me the most with the NIH is the lack of any public communication from any of its leaders about what is happening.
Reposted by Isletomics
I mean what an encyclopaedia of a paper was that by @soleimanpourlab.bsky.social and colleagues www.science.org/doi/10.1126/... amazing work and a compendium for mitochondrial biology in metabolic tissues. Have you seen this @heikolickert.bsky.social ? #PancSky
Retrograde mitochondrial signaling governs the identity and maturity of metabolic tissues
Mitochondrial damage is a hallmark of metabolic diseases, including diabetes, yet the consequences of compromised mitochondria in metabolic tissues are often unclear. Here, we report that dysfunctiona...
www.science.org
February 22, 2025 at 8:31 AM
I mean what an encyclopaedia of a paper was that by @soleimanpourlab.bsky.social and colleagues www.science.org/doi/10.1126/... amazing work and a compendium for mitochondrial biology in metabolic tissues. Have you seen this @heikolickert.bsky.social ? #PancSky
Reposted by Isletomics
Goosebump producing news this week out of Oregon Health and Science University in the diagnosis of pancreatic CA.
They developed a groundbreaking blood test, called PAC-MANN. This new test can find pancreatic cancer in just 45 minutes, even in its earliest stages.
They developed a groundbreaking blood test, called PAC-MANN. This new test can find pancreatic cancer in just 45 minutes, even in its earliest stages.
New blood test identifies hard-to-detect pancreatic cancer with 85% accuracy
A new blood test could help doctors detect pancreatic cancer earlier, potentially improving survival rates for one of the deadliest cancers.
www.eurekalert.org
February 22, 2025 at 2:21 PM
Goosebump producing news this week out of Oregon Health and Science University in the diagnosis of pancreatic CA.
They developed a groundbreaking blood test, called PAC-MANN. This new test can find pancreatic cancer in just 45 minutes, even in its earliest stages.
They developed a groundbreaking blood test, called PAC-MANN. This new test can find pancreatic cancer in just 45 minutes, even in its earliest stages.
Reposted by Isletomics
reminder: fancy model will not salvage your crappy data;
neither will fancy figures.
neither will fancy figures.
February 23, 2025 at 3:15 PM
reminder: fancy model will not salvage your crappy data;
neither will fancy figures.
neither will fancy figures.
Reposted by Isletomics
The current landscape and emerging challenges of benchmarking single-cell methods www.biorxiv.org/content/10....
February 22, 2025 at 2:45 PM
The current landscape and emerging challenges of benchmarking single-cell methods www.biorxiv.org/content/10....
Reposted by Isletomics
The Devil in Genomic Coordinates: 0-Based vs. 1-Based Systems
1/ The #1 cause of bioinformatics errors? Misunderstanding 0-based vs. 1-based coordinate systems. Let's break it down!
1/ The #1 cause of bioinformatics errors? Misunderstanding 0-based vs. 1-based coordinate systems. Let's break it down!
February 21, 2025 at 3:15 PM
The Devil in Genomic Coordinates: 0-Based vs. 1-Based Systems
1/ The #1 cause of bioinformatics errors? Misunderstanding 0-based vs. 1-based coordinate systems. Let's break it down!
1/ The #1 cause of bioinformatics errors? Misunderstanding 0-based vs. 1-based coordinate systems. Let's break it down!
Reposted by Isletomics
And lead of this work, @theodoreds.bsky.social just joined Bluesky. Give him a follow!
We link molecular and functional phenotypes of islet cells from donors with type 1 diabetes to understand impaired insulin and glucagon secretion. Immune signalling, nuclear exclusion, mTOR and lysosomes in alpha cell dysfunction.
https://www.biorxiv.org/content/10.1101/2025.02.20.639325v1
https://www.biorxiv.org/content/10.1101/2025.02.20.639325v1
February 21, 2025 at 4:01 PM
And lead of this work, @theodoreds.bsky.social just joined Bluesky. Give him a follow!
Reposted by Isletomics
How cool is this?!
🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
January 30, 2025 at 12:03 PM
How cool is this?!
Reposted by Isletomics
"Genes are... the easy part" @philipcball.bsky.social argues biologists need to embrace complexity from the start, rather than beginning with oversimplified "one gene, one trait" models
+1
www.cell.com/cell-systems...
+1
www.cell.com/cell-systems...
Should biology put complexity first?
The dictum “Everything should be made as simple as possible, but no simpler” poses
a problem for biology. How simply can it be told without doing damage to its complex
nature? The answer might be foun...
www.cell.com
February 21, 2025 at 7:44 AM
"Genes are... the easy part" @philipcball.bsky.social argues biologists need to embrace complexity from the start, rather than beginning with oversimplified "one gene, one trait" models
+1
www.cell.com/cell-systems...
+1
www.cell.com/cell-systems...
Reposted by Isletomics
Spatial integration of multi-omics single-cell data with SIMO www.nature.com/articles/s4...
February 20, 2025 at 2:45 PM
Spatial integration of multi-omics single-cell data with SIMO www.nature.com/articles/s4...
Reposted by Isletomics
Proud to share a new preprint from my lab that is the product of hard work from several talented lab members and collaboration with valued colleagues: www.biorxiv.org/content/10.1...
IFN-α Induces Heterogenous ROS Production in Human β-Cells
Type 1 diabetes (T1D) is a multifactorial disease involving genetic and environmental factors, including viral infection. We investigated the impact of interferon alpha (IFN-α), a cytokine produced du...
www.biorxiv.org
February 21, 2025 at 2:03 PM
Proud to share a new preprint from my lab that is the product of hard work from several talented lab members and collaboration with valued colleagues: www.biorxiv.org/content/10.1...