Juan C. Landoni
@jclandoni.bsky.social
Mitochondria | Translational Metabolism | Quantitative Imaging 🔬 (he/él)
jclandoni.github.io
jclandoni.github.io
Reposted by Juan C. Landoni
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Organelles harbour pH gradients https://www.biorxiv.org/content/10.64898/2025.12.12.694065v1
December 17, 2025 at 12:46 AM
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Reposted by Juan C. Landoni
🎸Why this is exciting!!🦖
Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.
This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.
This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
January 20, 2026 at 4:27 PM
🎸Why this is exciting!!🦖
Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.
This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.
This opens HUGE opportunities to count in tissues, organisms, or clinical samples!
Reposted by Juan C. Landoni
We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ!
We measured the abundances for Complex I, III, and IV.
How they differ in absolute abundances in metabolically distinct subpopulations
And even how T-cell activation changes supercomplex density
We measured the abundances for Complex I, III, and IV.
How they differ in absolute abundances in metabolically distinct subpopulations
And even how T-cell activation changes supercomplex density
January 20, 2026 at 4:24 PM
We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ!
We measured the abundances for Complex I, III, and IV.
How they differ in absolute abundances in metabolically distinct subpopulations
And even how T-cell activation changes supercomplex density
We measured the abundances for Complex I, III, and IV.
How they differ in absolute abundances in metabolically distinct subpopulations
And even how T-cell activation changes supercomplex density
Reposted by Juan C. Landoni
How does qExM work?
We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.
The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.
The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
January 20, 2026 at 4:15 PM
How does qExM work?
We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.
The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.
The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!
Reposted by Juan C. Landoni
We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge.
We achieved a mean error of just 9.4% 😍🔬🎸
With these tools in place we moved to tackle protein complex abundances that were unknown....
We achieved a mean error of just 9.4% 😍🔬🎸
With these tools in place we moved to tackle protein complex abundances that were unknown....
January 20, 2026 at 4:20 PM
We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge.
We achieved a mean error of just 9.4% 😍🔬🎸
With these tools in place we moved to tackle protein complex abundances that were unknown....
We achieved a mean error of just 9.4% 😍🔬🎸
With these tools in place we moved to tackle protein complex abundances that were unknown....
Reposted by Juan C. Landoni
🔬🚨New preprint alert! 🚨🔬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
www.biorxiv.org
January 20, 2026 at 4:12 PM
🔬🚨New preprint alert! 🚨🔬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
Reposted by Juan C. Landoni
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
November 4, 2025 at 10:40 PM
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬
December 27, 2025 at 11:57 AM
Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬
Reposted by Juan C. Landoni
The PIs of Tomorrow session is part of the LS² Annual Meeting 2026. We have now chosen our 4 finalists:
Theodora Constantin
Juan Cruz Landoni
Olimpia Bompadre
Alaz Özcan
More info: annual-meeting.ls2.ch/2026/special...
Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...
Theodora Constantin
Juan Cruz Landoni
Olimpia Bompadre
Alaz Özcan
More info: annual-meeting.ls2.ch/2026/special...
Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...
December 16, 2025 at 7:53 AM
The PIs of Tomorrow session is part of the LS² Annual Meeting 2026. We have now chosen our 4 finalists:
Theodora Constantin
Juan Cruz Landoni
Olimpia Bompadre
Alaz Özcan
More info: annual-meeting.ls2.ch/2026/special...
Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...
Theodora Constantin
Juan Cruz Landoni
Olimpia Bompadre
Alaz Özcan
More info: annual-meeting.ls2.ch/2026/special...
Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...
Reposted by Juan C. Landoni
🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
November 17, 2024 at 4:46 PM
🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
www.annualreviews.org/content/jour...
#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA
Reposted by Juan C. Landoni
#StarterPack for the #Mitochondria research community! Very much a work in progress, comment below/reach out to be added!
#Mito #Mitochondriac
go.bsky.app/J6Bu5fL
#Mito #Mitochondriac
go.bsky.app/J6Bu5fL
November 12, 2024 at 6:35 PM
#StarterPack for the #Mitochondria research community! Very much a work in progress, comment below/reach out to be added!
#Mito #Mitochondriac
go.bsky.app/J6Bu5fL
#Mito #Mitochondriac
go.bsky.app/J6Bu5fL
Reposted by Juan C. Landoni
Co-led with @jclandoni.bsky.social and fantastic support from Keaton Holt, Elena Koslover, @tatjanakleele.bsky.social , @sulianamanley.bsky.social , Sheda Ben Nejma, and Berna Durmus, you can find the preprint here: www.biorxiv.org/content/10.6...
It's wonderful to see this project out in the wild
It's wonderful to see this project out in the wild
Asymmetric mitochondrial trafficking maintains network morphology by balancing perinuclear biogenesis
In functionally polarized cells, mitochondria can form distinct subpopulations, positioned at sites of varying metabolic and energetic demands. Thus far, the potential presence of such subpopulations ...
www.biorxiv.org
December 11, 2025 at 1:02 PM
Co-led with @jclandoni.bsky.social and fantastic support from Keaton Holt, Elena Koslover, @tatjanakleele.bsky.social , @sulianamanley.bsky.social , Sheda Ben Nejma, and Berna Durmus, you can find the preprint here: www.biorxiv.org/content/10.6...
It's wonderful to see this project out in the wild
It's wonderful to see this project out in the wild
Reposted by Juan C. Landoni
Using photoconversion, volumetric imaging, mitochondrial segmentation, and network simulations, we identified a distinct, proliferative mitochondrial subpopulation positioned around the nucleus. From there, single mitochondria scatter new material across the cell, maintaining network in balance
December 11, 2025 at 1:02 PM
Using photoconversion, volumetric imaging, mitochondrial segmentation, and network simulations, we identified a distinct, proliferative mitochondrial subpopulation positioned around the nucleus. From there, single mitochondria scatter new material across the cell, maintaining network in balance
Reposted by Juan C. Landoni
🔬🥟 What do empanadas and #mitochondria have in common?
More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?
More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?
December 11, 2025 at 1:02 PM
🔬🥟 What do empanadas and #mitochondria have in common?
More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?
More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?
Preprint alert 🚨 Ever wondered what role fast #mitochondria trafficking plays in the simple cells we mostly use? In @julius-w.bsky.social et al. we use mitodynamics + simulations to identify the importance of outwards transport to distribute new material and keep network balance! Link below!
December 11, 2025 at 8:54 AM
Preprint alert 🚨 Ever wondered what role fast #mitochondria trafficking plays in the simple cells we mostly use? In @julius-w.bsky.social et al. we use mitodynamics + simulations to identify the importance of outwards transport to distribute new material and keep network balance! Link below!
Tune in! 🔬
At this week's #VirtualPub, we welcome Juan Cruz Landoni, EPFL, on "Pearling drives mitochondrial DNA nucleoid homeostasis."
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
December 10, 2025 at 1:40 PM
Tune in! 🔬
Reposted by Juan C. Landoni
At this week's #VirtualPub, we welcome Juan Cruz Landoni, EPFL, on "Pearling drives mitochondrial DNA nucleoid homeostasis."
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
December 10, 2025 at 1:32 PM
At this week's #VirtualPub, we welcome Juan Cruz Landoni, EPFL, on "Pearling drives mitochondrial DNA nucleoid homeostasis."
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...
Reposted by Juan C. Landoni
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
November 24, 2025 at 4:57 PM
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
It's not #FluorescenceFriday but unexpectedly beautiful data should always be celebrated 💚 #PKMO -stained #mitochondria cristae in #STED
November 25, 2025 at 12:56 PM
It's not #FluorescenceFriday but unexpectedly beautiful data should always be celebrated 💚 #PKMO -stained #mitochondria cristae in #STED
Another year and another group of brilliant young students preparing for the International Biology Olympiad #IBO 💪☘️
Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!
Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!
November 21, 2025 at 10:01 AM
Another year and another group of brilliant young students preparing for the International Biology Olympiad #IBO 💪☘️
Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!
Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!
Smartest booth luring strategy I've seen: a pro barista on site 🤯
Drop the free pens. Science runs on espresso ☕️
Drop the free pens. Science runs on espresso ☕️
November 6, 2025 at 12:24 PM
Smartest booth luring strategy I've seen: a pro barista on site 🤯
Drop the free pens. Science runs on espresso ☕️
Drop the free pens. Science runs on espresso ☕️
That's a wrap on a much-needed family vacay, thank you Costa Rica for your incredible nature 🌋🦥🏞️🐠🐡🐒🦈, now back to our regularly scheduled programming 🔬
October 27, 2025 at 3:31 PM
That's a wrap on a much-needed family vacay, thank you Costa Rica for your incredible nature 🌋🦥🏞️🐠🐡🐒🦈, now back to our regularly scheduled programming 🔬
Reposted by Juan C. Landoni
New paper - MAPL strikes again! Interested in mitochondrial signalling, inflammation, lysosome biology, pyroptosis, and Parkinson's disease? Have a look, there's something for everyone! Feeling grateful! @mitocollier.bsky.social Funded by #CIHR, @asapresearch.parkinsonsroadmap.org.
rdcu.be/eKKz1 🇨🇦
rdcu.be/eKKz1 🇨🇦
October 14, 2025 at 11:50 AM
New paper - MAPL strikes again! Interested in mitochondrial signalling, inflammation, lysosome biology, pyroptosis, and Parkinson's disease? Have a look, there's something for everyone! Feeling grateful! @mitocollier.bsky.social Funded by #CIHR, @asapresearch.parkinsonsroadmap.org.
rdcu.be/eKKz1 🇨🇦
rdcu.be/eKKz1 🇨🇦