Darko Barisic
@darkobar.bsky.social
Assistant Professor of Cancer Biology at Weill Cornell Medicine — NIH NCI R00 Funded — Chromatin & Epigenetics in Cancer and Immunity.
Pinned
Darko Barisic
@darkobar.bsky.social
· Nov 14
Thrilled to share we are @theNCI R00-funded. 🥳 Looking for computational biologists or bioinformaticians. 👩💻👨💻 We have many exciting projects on the role of chromatin remodelers in blood cancers. 🩸🦀 Please share/DM/email if relevant. Our latest story: www.cell.com/cancer-cell/...
Building a network of lymphoma researchers on @bsky.app. Let us know who we’re missing! 🔬
go.bsky.app/KbjoCuC
go.bsky.app/KbjoCuC
August 30, 2025 at 2:58 PM
Building a network of lymphoma researchers on @bsky.app. Let us know who we’re missing! 🔬
go.bsky.app/KbjoCuC
go.bsky.app/KbjoCuC
Lab night out on Broadway! 🎭 A well-earned pause from experiments. 🧬
July 13, 2025 at 2:27 PM
Lab night out on Broadway! 🎭 A well-earned pause from experiments. 🧬
Huge congratulations to my postdoc Jiali Yu 👏 who has been awarded a fellowship from the National Cancer Center. She’ll be investigating how chromatin remodelers contribute to the onset and progression of blood cancers. So proud and excited for what’s ahead. 🚀
June 14, 2025 at 1:59 PM
Huge congratulations to my postdoc Jiali Yu 👏 who has been awarded a fellowship from the National Cancer Center. She’ll be investigating how chromatin remodelers contribute to the onset and progression of blood cancers. So proud and excited for what’s ahead. 🚀
Huge congrats to *Ari Melnick* on being appointed the Director of the Josep Carreras Leukemia Research Institute in Barcelona! 🙌 👏
We're pleased to announce that Dr Ari Melnick has been appointed new Director of the Josep Carreras Institute.
Dr Melnick will lead IJC in our mission to advance #research in #leukaemia and other malignant blood diseases.
Read the full release here: www.carrerasresearch.org/en/news/dr-a...
Dr Melnick will lead IJC in our mission to advance #research in #leukaemia and other malignant blood diseases.
Read the full release here: www.carrerasresearch.org/en/news/dr-a...
April 22, 2025 at 3:28 PM
Huge congrats to *Ari Melnick* on being appointed the Director of the Josep Carreras Leukemia Research Institute in Barcelona! 🙌 👏
Reposted by Darko Barisic
🎉 Excited to share my postdoc work in the Bernstein lab now online @cp-molcell.bsky.social!
🔎We explored the unique functions of the SWI/SNF PBAF complex and its relationship with TFs, and unveiled a PBAF/REST-controlled neuronal-like signature with potential implications in #melanoma brain mets🧬
🔎We explored the unique functions of the SWI/SNF PBAF complex and its relationship with TFs, and unveiled a PBAF/REST-controlled neuronal-like signature with potential implications in #melanoma brain mets🧬
The SWI/SNF PBAF complex facilitates REST occupancy at repressive chromatin
By mapping SWI/SNF complex subfamilies in the melanocytic lineage, Grossi and Nguyen
et al. find that PBAF-only sites harbor PRC1/2 modifications and the repressive transcription
factor REST. PBAF is ...
www.cell.com
April 21, 2025 at 12:38 PM
🎉 Excited to share my postdoc work in the Bernstein lab now online @cp-molcell.bsky.social!
🔎We explored the unique functions of the SWI/SNF PBAF complex and its relationship with TFs, and unveiled a PBAF/REST-controlled neuronal-like signature with potential implications in #melanoma brain mets🧬
🔎We explored the unique functions of the SWI/SNF PBAF complex and its relationship with TFs, and unveiled a PBAF/REST-controlled neuronal-like signature with potential implications in #melanoma brain mets🧬
Reposted by Darko Barisic
🚨Our new prepint 🚨
▶️ When a bromodomain goes rogue
Rsc1 bromodomain 2 doesn’t bind acetyl-lysines.
It grabs DNA instead — and yeast cells die 💀 without it.
Plot twist? Read our preprint:
👉 www.biorxiv.org/content/10.1...
#chromatin #bromodrama #DNAlover
And a 🧵👇 1/5
▶️ When a bromodomain goes rogue
Rsc1 bromodomain 2 doesn’t bind acetyl-lysines.
It grabs DNA instead — and yeast cells die 💀 without it.
Plot twist? Read our preprint:
👉 www.biorxiv.org/content/10.1...
#chromatin #bromodrama #DNAlover
And a 🧵👇 1/5
March 28, 2025 at 10:47 AM
🚨Our new prepint 🚨
▶️ When a bromodomain goes rogue
Rsc1 bromodomain 2 doesn’t bind acetyl-lysines.
It grabs DNA instead — and yeast cells die 💀 without it.
Plot twist? Read our preprint:
👉 www.biorxiv.org/content/10.1...
#chromatin #bromodrama #DNAlover
And a 🧵👇 1/5
▶️ When a bromodomain goes rogue
Rsc1 bromodomain 2 doesn’t bind acetyl-lysines.
It grabs DNA instead — and yeast cells die 💀 without it.
Plot twist? Read our preprint:
👉 www.biorxiv.org/content/10.1...
#chromatin #bromodrama #DNAlover
And a 🧵👇 1/5
Absolutely fantastic work from the magnificent @davidlabmsk.bsky.social lab! Incredible science and amazing out-of-the-box approach. Congrats to all the authors and highly recommend the read. 🙌
I am extremely proud to see our work about Hepatitis B now published in @cellpress.bsky.social
From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵
www.cell.com/cell/fulltex...
From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵
www.cell.com/cell/fulltex...
A nucleosome switch primes hepatitis B virus infection
A reconstituted cccDNA platform facilitated the discovery of an early chromatinization
event that promotes transcription of the hepatitis B virus (HBV) regulatory X gene.
The use of a small-molecule c...
www.cell.com
February 24, 2025 at 2:12 AM
Absolutely fantastic work from the magnificent @davidlabmsk.bsky.social lab! Incredible science and amazing out-of-the-box approach. Congrats to all the authors and highly recommend the read. 🙌
Reposted by Darko Barisic
Nearly every medicine we benefit from started with NIH-funded research. Early discovery work starts in academia.
Immunotherapies?
Gene therapy for rare diseases?
Alzheimer’s and Parkinson’s medicines?
Vaccines?
Novel cancer treatments?
Psychiatric medicines?
You betcha.
1/
Immunotherapies?
Gene therapy for rare diseases?
Alzheimer’s and Parkinson’s medicines?
Vaccines?
Novel cancer treatments?
Psychiatric medicines?
You betcha.
1/
February 12, 2025 at 5:35 PM
Nearly every medicine we benefit from started with NIH-funded research. Early discovery work starts in academia.
Immunotherapies?
Gene therapy for rare diseases?
Alzheimer’s and Parkinson’s medicines?
Vaccines?
Novel cancer treatments?
Psychiatric medicines?
You betcha.
1/
Immunotherapies?
Gene therapy for rare diseases?
Alzheimer’s and Parkinson’s medicines?
Vaccines?
Novel cancer treatments?
Psychiatric medicines?
You betcha.
1/
Impressed by Genome-Shuffle-seq. 🔀 “Multiplex generation and single-cell analysis of structural variants in mammalian genomes”.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Multiplex generation and single-cell analysis of structural variants in mammalian genomes
Studying the functional consequences of structural variants (SVs) in mammalian genomes is challenging because (i) SVs arise much less commonly than single-nucleotide variants or small indels and (ii) ...
www.science.org
February 7, 2025 at 3:43 AM
Impressed by Genome-Shuffle-seq. 🔀 “Multiplex generation and single-cell analysis of structural variants in mammalian genomes”.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Long-read sequencing just got an upgrade. 📢 Genome, methylome, epigenome & transcriptome in one go—haplotype-resolved & at single-molecule resolution. 🔥
The "kitchen sink" of omics to solve the basis for an undiagnosed disease: long read genome , transcriptome, methytome, epigenome, all synchronized (a first)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Synchronized long-read genome, methylome, epigenome and transcriptome profiling resolve a Mendelian condition - Nature Genetics
Simultaneous profiling of the genome, methylome, epigenome and transcriptome using single-molecule chromatin fiber sequencing and multiplexed arrays isoform sequencing identifies the genetic and molec...
www.nature.com
January 30, 2025 at 5:00 AM
Long-read sequencing just got an upgrade. 📢 Genome, methylome, epigenome & transcriptome in one go—haplotype-resolved & at single-molecule resolution. 🔥
PRINT is finally out. 🖨️ Highly recommend this paper! Congrats to Buenrostro group and all the authors. 🙌 “Multiscale footprints reveal the organization of cis-regulatory elements.”
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Multiscale footprints reveal the organization of cis-regulatory elements - Nature
We developed PRINT, a computational method that identifies footprints of DNA–protein interactions from bulk and single-cell chromatin accessibility data across multiple scales of protein size.
www.nature.com
January 27, 2025 at 10:04 PM
PRINT is finally out. 🖨️ Highly recommend this paper! Congrats to Buenrostro group and all the authors. 🙌 “Multiscale footprints reveal the organization of cis-regulatory elements.”
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Darko Barisic
🧬 We’re excited to introduce D&D-seq, a single-cell technology that maps DNA:Protein interactions through molecular footprinting. Check it out here: biorxiv.org/content/10.1... #Genomics #Epigenetics
Single-cell mapping of regulatory DNA:Protein interactions
Gene expression is coordinated by a multitude of transcription factors (TFs), whose binding to the genome is directed through multiple interconnected epigenetic signals, including chromatin accessibil...
biorxiv.org
January 2, 2025 at 9:10 PM
🧬 We’re excited to introduce D&D-seq, a single-cell technology that maps DNA:Protein interactions through molecular footprinting. Check it out here: biorxiv.org/content/10.1... #Genomics #Epigenetics
#ASH2024 Highly recommend this place for lunch. Also, highly recommend the company @jchellmuth.bsky.social!
December 8, 2024 at 8:25 PM
#ASH2024 Highly recommend this place for lunch. Also, highly recommend the company @jchellmuth.bsky.social!
Reposted by Darko Barisic
December 8, 2024 at 3:33 AM
Huge congrats to Béguelin lab at Weill Cornell. Fantastic new work showing how PRC2 inhibition boosts T cell immunotherapies. Out now in Cancer Cell.
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...
EZH2 inhibition enhances T cell immunotherapies by inducing lymphoma immunogenicity and improving T cell function
Isshiki et al. demonstrate that EZH2 inhibitors re-activate immune synapse functions between B cell lymphomas and T cells, sensitizing lymphomas to T cell immunotherapies. EZH2 inhibition enhances killing activity by promoting T cell recruitment, prolonging binding and increasing contact surface with lymphoma cells, and prevents T cell exhaustion. This strategy is currently under clinical evaluation.
www.cell.com
December 5, 2024 at 4:23 PM
Huge congrats to Béguelin lab at Weill Cornell. Fantastic new work showing how PRC2 inhibition boosts T cell immunotherapies. Out now in Cancer Cell.
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...
Reposted by Darko Barisic
Good news, Altmetric has now started watching BlueSky for mentions of publications. And by the way, provides an easy comparison between this and the old site for a recent preprint of mine which I posted simultaneousl at both. Numbers speak by themselves !
December 2, 2024 at 6:35 PM
Good news, Altmetric has now started watching BlueSky for mentions of publications. And by the way, provides an easy comparison between this and the old site for a recent preprint of mine which I posted simultaneousl at both. Numbers speak by themselves !
Reposted by Darko Barisic
H3-H4 histone dimers act as copper reductase. This is important because ETC and SOD1 require bio-usable Cu+ for function. This article shows a new mechanism by which chromatin histones can affect the cell metabolism via a new role in metal processing #redoxepigenetics www.science.org/doi/10.1126/...
The histone H3-H4 tetramer is a copper reductase enzyme
A eukaryotic histone complex can package DNA but is also a cupric reductase that provides biousable Cu1+ for the cell.
www.science.org
December 1, 2024 at 1:34 PM
H3-H4 histone dimers act as copper reductase. This is important because ETC and SOD1 require bio-usable Cu+ for function. This article shows a new mechanism by which chromatin histones can affect the cell metabolism via a new role in metal processing #redoxepigenetics www.science.org/doi/10.1126/...
Characterizing the evolutionary dynamics of cancer proliferation in single-cell clones with SPRINTER.
doi.org/10.1038/s415...
doi.org/10.1038/s415...
Characterizing the evolutionary dynamics of cancer proliferation in single-cell clones with SPRINTER - Nature Genetics
Single-cell Proliferation Rate Inference in Non-homogeneous Tumors through Evolutionary Routes (SPRINTER) allows users to infer proliferation rates of individual clones within a tumor from single-cell...
doi.org
November 30, 2024 at 6:10 PM
Characterizing the evolutionary dynamics of cancer proliferation in single-cell clones with SPRINTER.
doi.org/10.1038/s415...
doi.org/10.1038/s415...
Happy Thanksgiving from Weill Cornell fourteen floor groups! 🦃 🇮🇹 🇨🇳 🇦🇷 🇺🇸 🇭🇷 🇷🇺 🇵🇱 🇺🇿 🇰🇷 🇫🇷 🇬🇷
November 28, 2024 at 7:38 PM
Happy Thanksgiving from Weill Cornell fourteen floor groups! 🦃 🇮🇹 🇨🇳 🇦🇷 🇺🇸 🇭🇷 🇷🇺 🇵🇱 🇺🇿 🇰🇷 🇫🇷 🇬🇷
Please help me curate a Lymphoma Research Starter Pack by suggesting folks to add or messaging me to be added yourself. Looking forward to more lymphoma topics on BlueSky. 🙌
November 26, 2024 at 6:00 PM
Please help me curate a Lymphoma Research Starter Pack by suggesting folks to add or messaging me to be added yourself. Looking forward to more lymphoma topics on BlueSky. 🙌
Reposted by Darko Barisic
Very interesting paper from @mitchguttman.bsky.social previously in @biorxivpreprint.bsky.social out in Nature Genetics. "ChIP-DIP generates context-specific protein localization maps at consortium scale"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements - Nature Genetics
ChIP-DIP (ChIP done in parallel) is a highly multiplex assay for protein–DNA binding, scalable to hundreds of proteins including modified histones, chromatin regulators and transcription factors, offe...
www.nature.com
November 25, 2024 at 4:59 PM
Very interesting paper from @mitchguttman.bsky.social previously in @biorxivpreprint.bsky.social out in Nature Genetics. "ChIP-DIP generates context-specific protein localization maps at consortium scale"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Darko Barisic
starter pack 2 for all things #chromatin, #transcription, and adjacent: go.bsky.app/Q5sXc6E - reply to self-nominate or suggest colleagues!
check out pack 1: go.bsky.app/5zgpZfg and @sproullab.bsky.social's pack: go.bsky.app/TKp9YAn for more people to follow
check out pack 1: go.bsky.app/5zgpZfg and @sproullab.bsky.social's pack: go.bsky.app/TKp9YAn for more people to follow
November 13, 2024 at 2:56 PM
starter pack 2 for all things #chromatin, #transcription, and adjacent: go.bsky.app/Q5sXc6E - reply to self-nominate or suggest colleagues!
check out pack 1: go.bsky.app/5zgpZfg and @sproullab.bsky.social's pack: go.bsky.app/TKp9YAn for more people to follow
check out pack 1: go.bsky.app/5zgpZfg and @sproullab.bsky.social's pack: go.bsky.app/TKp9YAn for more people to follow
Reposted by Darko Barisic
www.nature.com/articles/s41...
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression - Nature
A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor bind...
www.nature.com
November 21, 2024 at 4:15 PM
www.nature.com/articles/s41...
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers - Nature Structural & Molecular Biology
Here, the authors show that the canonical BRG/BRM-associated factor (SWI/SNF) chromatin remodeler generates subnucleosomes containing 50–80 bp of DNA associated with the four core histones. These hemi...
www.nature.com
November 20, 2024 at 6:05 PM
cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers.
www.nature.com/articles/s41...
www.nature.com/articles/s41...